Amino acid dipepetide frequency for Lactococcus phage AM5

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.187AlaAla: 0.187 ± 0.099
0.241AlaCys: 0.241 ± 0.079
2.594AlaAsp: 2.594 ± 0.273
3.529AlaGlu: 3.529 ± 0.31
1.765AlaPhe: 1.765 ± 0.25
3.663AlaGly: 3.663 ± 0.625
0.802AlaHis: 0.802 ± 0.144
3.663AlaIle: 3.663 ± 0.405
5.107AlaLys: 5.107 ± 0.59
4.572AlaLeu: 4.572 ± 0.522
1.176AlaMet: 1.176 ± 0.176
3.289AlaAsn: 3.289 ± 0.3
0.963AlaPro: 0.963 ± 0.207
2.406AlaGln: 2.406 ± 0.434
1.765AlaArg: 1.765 ± 0.246
3.69AlaSer: 3.69 ± 0.624
3.182AlaThr: 3.182 ± 0.518
3.69AlaVal: 3.69 ± 0.479
0.535AlaTrp: 0.535 ± 0.138
2.888AlaTyr: 2.888 ± 0.301
0.0AlaXaa: 0.0 ± 0.0
Cys
0.16CysAla: 0.16 ± 0.061
0.08CysCys: 0.08 ± 0.042
0.428CysAsp: 0.428 ± 0.112
0.481CysGlu: 0.481 ± 0.127
0.348CysPhe: 0.348 ± 0.112
0.508CysGly: 0.508 ± 0.144
0.027CysHis: 0.027 ± 0.027
0.481CysIle: 0.481 ± 0.12
0.722CysLys: 0.722 ± 0.174
0.374CysLeu: 0.374 ± 0.088
0.107CysMet: 0.107 ± 0.049
0.508CysAsn: 0.508 ± 0.129
0.374CysPro: 0.374 ± 0.169
0.214CysGln: 0.214 ± 0.09
0.428CysArg: 0.428 ± 0.103
0.588CysSer: 0.588 ± 0.126
0.374CysThr: 0.374 ± 0.117
0.508CysVal: 0.508 ± 0.137
0.08CysTrp: 0.08 ± 0.047
0.267CysTyr: 0.267 ± 0.089
0.0CysXaa: 0.0 ± 0.0
Asp
2.834AspAla: 2.834 ± 0.463
0.267AspCys: 0.267 ± 0.084
4.439AspAsp: 4.439 ± 0.321
6.096AspGlu: 6.096 ± 0.439
4.305AspPhe: 4.305 ± 0.4
4.706AspGly: 4.706 ± 0.392
0.481AspHis: 0.481 ± 0.13
5.856AspIle: 5.856 ± 0.412
7.005AspLys: 7.005 ± 0.486
5.642AspLeu: 5.642 ± 0.456
1.658AspMet: 1.658 ± 0.216
4.759AspAsn: 4.759 ± 0.35
0.829AspPro: 0.829 ± 0.133
0.963AspGln: 0.963 ± 0.2
1.979AspArg: 1.979 ± 0.286
4.278AspSer: 4.278 ± 0.446
4.064AspThr: 4.064 ± 0.343
3.85AspVal: 3.85 ± 0.3
0.802AspTrp: 0.802 ± 0.144
2.995AspTyr: 2.995 ± 0.318
0.0AspXaa: 0.0 ± 0.0
Glu
4.037GluAla: 4.037 ± 0.401
0.455GluCys: 0.455 ± 0.11
5.722GluAsp: 5.722 ± 0.567
6.925GluGlu: 6.925 ± 0.588
3.396GluPhe: 3.396 ± 0.431
3.663GluGly: 3.663 ± 0.26
1.283GluHis: 1.283 ± 0.213
7.193GluIle: 7.193 ± 0.491
7.326GluLys: 7.326 ± 0.489
7.834GluLeu: 7.834 ± 0.457
2.112GluMet: 2.112 ± 0.219
4.652GluAsn: 4.652 ± 0.359
1.096GluPro: 1.096 ± 0.221
2.594GluGln: 2.594 ± 0.261
2.487GluArg: 2.487 ± 0.299
5.16GluSer: 5.16 ± 0.326
3.77GluThr: 3.77 ± 0.318
4.786GluVal: 4.786 ± 0.408
0.722GluTrp: 0.722 ± 0.137
4.358GluTyr: 4.358 ± 0.504
0.0GluXaa: 0.0 ± 0.0
Phe
2.219PheAla: 2.219 ± 0.257
0.401PheCys: 0.401 ± 0.119
3.77PheAsp: 3.77 ± 0.314
3.85PheGlu: 3.85 ± 0.367
1.497PhePhe: 1.497 ± 0.255
2.781PheGly: 2.781 ± 0.249
0.642PheHis: 0.642 ± 0.169
3.075PheIle: 3.075 ± 0.287
4.144PheLys: 4.144 ± 0.385
3.289PheLeu: 3.289 ± 0.384
1.364PheMet: 1.364 ± 0.177
2.995PheAsn: 2.995 ± 0.315
0.722PhePro: 0.722 ± 0.146
1.31PheGln: 1.31 ± 0.181
1.604PheArg: 1.604 ± 0.263
2.914PheSer: 2.914 ± 0.363
2.995PheThr: 2.995 ± 0.252
2.46PheVal: 2.46 ± 0.272
0.481PheTrp: 0.481 ± 0.101
2.005PheTyr: 2.005 ± 0.328
0.0PheXaa: 0.0 ± 0.0
Gly
2.674GlyAla: 2.674 ± 0.413
0.374GlyCys: 0.374 ± 0.102
3.61GlyAsp: 3.61 ± 0.281
3.957GlyGlu: 3.957 ± 0.314
3.128GlyPhe: 3.128 ± 0.267
3.422GlyGly: 3.422 ± 0.52
0.909GlyHis: 0.909 ± 0.155
4.92GlyIle: 4.92 ± 0.359
4.947GlyLys: 4.947 ± 0.342
4.545GlyLeu: 4.545 ± 0.381
1.497GlyMet: 1.497 ± 0.2
3.957GlyAsn: 3.957 ± 0.433
0.134GlyPro: 0.134 ± 0.059
1.497GlyGln: 1.497 ± 0.184
2.219GlyArg: 2.219 ± 0.249
3.663GlySer: 3.663 ± 0.345
3.957GlyThr: 3.957 ± 0.361
3.904GlyVal: 3.904 ± 0.237
0.802GlyTrp: 0.802 ± 0.161
3.262GlyTyr: 3.262 ± 0.297
0.0GlyXaa: 0.0 ± 0.0
His
0.695HisAla: 0.695 ± 0.132
0.08HisCys: 0.08 ± 0.046
1.07HisAsp: 1.07 ± 0.145
1.096HisGlu: 1.096 ± 0.211
0.642HisPhe: 0.642 ± 0.149
1.07HisGly: 1.07 ± 0.208
0.214HisHis: 0.214 ± 0.077
1.23HisIle: 1.23 ± 0.208
1.15HisLys: 1.15 ± 0.17
1.123HisLeu: 1.123 ± 0.196
0.401HisMet: 0.401 ± 0.101
0.989HisAsn: 0.989 ± 0.225
0.348HisPro: 0.348 ± 0.111
0.535HisGln: 0.535 ± 0.129
0.508HisArg: 0.508 ± 0.121
0.989HisSer: 0.989 ± 0.177
0.775HisThr: 0.775 ± 0.116
0.588HisVal: 0.588 ± 0.114
0.187HisTrp: 0.187 ± 0.073
0.722HisTyr: 0.722 ± 0.154
0.0HisXaa: 0.0 ± 0.0
Ile
3.743IleAla: 3.743 ± 0.36
0.481IleCys: 0.481 ± 0.123
5.775IleAsp: 5.775 ± 0.326
6.123IleGlu: 6.123 ± 0.497
3.102IlePhe: 3.102 ± 0.387
3.636IleGly: 3.636 ± 0.416
1.016IleHis: 1.016 ± 0.174
5.401IleIle: 5.401 ± 0.519
7.674IleLys: 7.674 ± 0.456
5.642IleLeu: 5.642 ± 0.525
1.471IleMet: 1.471 ± 0.193
5.749IleAsn: 5.749 ± 0.363
2.513IlePro: 2.513 ± 0.347
2.513IleGln: 2.513 ± 0.372
2.273IleArg: 2.273 ± 0.245
5.535IleSer: 5.535 ± 0.334
4.626IleThr: 4.626 ± 0.432
5.214IleVal: 5.214 ± 0.467
0.642IleTrp: 0.642 ± 0.121
3.075IleTyr: 3.075 ± 0.36
0.0IleXaa: 0.0 ± 0.0
Lys
5.08LysAla: 5.08 ± 0.618
0.829LysCys: 0.829 ± 0.234
6.31LysAsp: 6.31 ± 0.417
9.679LysGlu: 9.679 ± 0.518
3.877LysPhe: 3.877 ± 0.395
4.278LysGly: 4.278 ± 0.286
1.604LysHis: 1.604 ± 0.273
6.872LysIle: 6.872 ± 0.349
8.048LysLys: 8.048 ± 0.512
8.369LysLeu: 8.369 ± 0.464
2.995LysMet: 2.995 ± 0.282
5.936LysAsn: 5.936 ± 0.383
2.086LysPro: 2.086 ± 0.229
3.235LysGln: 3.235 ± 0.376
3.69LysArg: 3.69 ± 0.346
4.786LysSer: 4.786 ± 0.523
5.267LysThr: 5.267 ± 0.357
5.08LysVal: 5.08 ± 0.302
1.096LysTrp: 1.096 ± 0.162
3.824LysTyr: 3.824 ± 0.319
0.0LysXaa: 0.0 ± 0.0
Leu
4.92LeuAla: 4.92 ± 0.385
0.749LeuCys: 0.749 ± 0.176
5.642LeuAsp: 5.642 ± 0.384
6.417LeuGlu: 6.417 ± 0.479
3.503LeuPhe: 3.503 ± 0.441
5.134LeuGly: 5.134 ± 0.466
1.043LeuHis: 1.043 ± 0.179
5.829LeuIle: 5.829 ± 0.471
8.342LeuLys: 8.342 ± 0.596
6.283LeuLeu: 6.283 ± 0.486
1.925LeuMet: 1.925 ± 0.201
5.561LeuAsn: 5.561 ± 0.369
2.166LeuPro: 2.166 ± 0.22
2.701LeuGln: 2.701 ± 0.383
2.594LeuArg: 2.594 ± 0.292
6.176LeuSer: 6.176 ± 0.498
5.428LeuThr: 5.428 ± 0.406
4.572LeuVal: 4.572 ± 0.39
0.615LeuTrp: 0.615 ± 0.118
3.209LeuTyr: 3.209 ± 0.377
0.0LeuXaa: 0.0 ± 0.0
Met
1.658MetAla: 1.658 ± 0.215
0.134MetCys: 0.134 ± 0.064
1.631MetAsp: 1.631 ± 0.249
1.684MetGlu: 1.684 ± 0.236
0.936MetPhe: 0.936 ± 0.166
1.283MetGly: 1.283 ± 0.224
0.134MetHis: 0.134 ± 0.056
1.631MetIle: 1.631 ± 0.18
2.594MetLys: 2.594 ± 0.235
1.711MetLeu: 1.711 ± 0.233
0.695MetMet: 0.695 ± 0.115
2.086MetAsn: 2.086 ± 0.197
0.802MetPro: 0.802 ± 0.152
0.909MetGln: 0.909 ± 0.244
0.856MetArg: 0.856 ± 0.156
1.925MetSer: 1.925 ± 0.299
1.791MetThr: 1.791 ± 0.217
1.497MetVal: 1.497 ± 0.164
0.267MetTrp: 0.267 ± 0.088
1.123MetTyr: 1.123 ± 0.203
0.0MetXaa: 0.0 ± 0.0
Asn
3.235AsnAla: 3.235 ± 0.433
0.374AsnCys: 0.374 ± 0.099
3.61AsnAsp: 3.61 ± 0.289
5.16AsnGlu: 5.16 ± 0.396
2.433AsnPhe: 2.433 ± 0.287
4.305AsnGly: 4.305 ± 0.326
0.936AsnHis: 0.936 ± 0.182
5.16AsnIle: 5.16 ± 0.369
6.176AsnLys: 6.176 ± 0.443
5.722AsnLeu: 5.722 ± 0.46
1.31AsnMet: 1.31 ± 0.157
3.69AsnAsn: 3.69 ± 0.348
2.032AsnPro: 2.032 ± 0.199
2.888AsnGln: 2.888 ± 0.398
2.193AsnArg: 2.193 ± 0.296
4.519AsnSer: 4.519 ± 0.361
3.717AsnThr: 3.717 ± 0.285
4.037AsnVal: 4.037 ± 0.349
0.588AsnTrp: 0.588 ± 0.121
2.968AsnTyr: 2.968 ± 0.248
0.0AsnXaa: 0.0 ± 0.0
Pro
1.043ProAla: 1.043 ± 0.152
0.08ProCys: 0.08 ± 0.046
1.765ProAsp: 1.765 ± 0.221
1.471ProGlu: 1.471 ± 0.153
1.096ProPhe: 1.096 ± 0.205
0.08ProGly: 0.08 ± 0.061
0.374ProHis: 0.374 ± 0.097
1.604ProIle: 1.604 ± 0.188
2.193ProLys: 2.193 ± 0.346
1.952ProLeu: 1.952 ± 0.223
0.695ProMet: 0.695 ± 0.14
1.658ProAsn: 1.658 ± 0.293
0.321ProPro: 0.321 ± 0.093
0.668ProGln: 0.668 ± 0.129
0.561ProArg: 0.561 ± 0.139
1.471ProSer: 1.471 ± 0.236
1.684ProThr: 1.684 ± 0.268
1.952ProVal: 1.952 ± 0.262
0.134ProTrp: 0.134 ± 0.071
1.39ProTyr: 1.39 ± 0.228
0.0ProXaa: 0.0 ± 0.0
Gln
1.979GlnAla: 1.979 ± 0.419
0.187GlnCys: 0.187 ± 0.065
1.738GlnAsp: 1.738 ± 0.177
2.193GlnGlu: 2.193 ± 0.224
1.39GlnPhe: 1.39 ± 0.2
1.925GlnGly: 1.925 ± 0.256
0.829GlnHis: 0.829 ± 0.161
2.406GlnIle: 2.406 ± 0.242
2.807GlnLys: 2.807 ± 0.382
3.262GlnLeu: 3.262 ± 0.448
1.257GlnMet: 1.257 ± 0.324
1.952GlnAsn: 1.952 ± 0.242
0.749GlnPro: 0.749 ± 0.157
1.524GlnGln: 1.524 ± 0.519
1.578GlnArg: 1.578 ± 0.193
1.791GlnSer: 1.791 ± 0.283
2.406GlnThr: 2.406 ± 0.402
1.39GlnVal: 1.39 ± 0.199
0.348GlnTrp: 0.348 ± 0.105
1.684GlnTyr: 1.684 ± 0.21
0.0GlnXaa: 0.0 ± 0.0
Arg
1.31ArgAla: 1.31 ± 0.173
0.401ArgCys: 0.401 ± 0.177
2.567ArgAsp: 2.567 ± 0.232
2.941ArgGlu: 2.941 ± 0.265
1.631ArgPhe: 1.631 ± 0.183
2.166ArgGly: 2.166 ± 0.249
0.535ArgHis: 0.535 ± 0.13
2.513ArgIle: 2.513 ± 0.305
3.128ArgLys: 3.128 ± 0.341
2.513ArgLeu: 2.513 ± 0.289
0.802ArgMet: 0.802 ± 0.134
2.513ArgAsn: 2.513 ± 0.253
0.561ArgPro: 0.561 ± 0.136
1.203ArgGln: 1.203 ± 0.205
1.043ArgArg: 1.043 ± 0.173
1.791ArgSer: 1.791 ± 0.25
1.979ArgThr: 1.979 ± 0.242
2.353ArgVal: 2.353 ± 0.272
0.535ArgTrp: 0.535 ± 0.121
2.166ArgTyr: 2.166 ± 0.19
0.0ArgXaa: 0.0 ± 0.0
Ser
3.717SerAla: 3.717 ± 0.56
0.455SerCys: 0.455 ± 0.123
4.572SerAsp: 4.572 ± 0.453
5.053SerGlu: 5.053 ± 0.313
3.396SerPhe: 3.396 ± 0.299
4.305SerGly: 4.305 ± 0.482
0.856SerHis: 0.856 ± 0.201
5.508SerIle: 5.508 ± 0.509
5.989SerLys: 5.989 ± 0.417
5.134SerLeu: 5.134 ± 0.603
1.444SerMet: 1.444 ± 0.167
3.476SerAsn: 3.476 ± 0.419
1.578SerPro: 1.578 ± 0.2
2.032SerGln: 2.032 ± 0.239
2.487SerArg: 2.487 ± 0.241
5.08SerSer: 5.08 ± 0.702
4.011SerThr: 4.011 ± 0.359
4.064SerVal: 4.064 ± 0.307
0.695SerTrp: 0.695 ± 0.167
2.406SerTyr: 2.406 ± 0.247
0.0SerXaa: 0.0 ± 0.0
Thr
3.663ThrAla: 3.663 ± 0.59
0.401ThrCys: 0.401 ± 0.152
4.011ThrAsp: 4.011 ± 0.284
3.636ThrGlu: 3.636 ± 0.387
2.807ThrPhe: 2.807 ± 0.326
3.61ThrGly: 3.61 ± 0.461
1.016ThrHis: 1.016 ± 0.197
4.599ThrIle: 4.599 ± 0.416
5.428ThrLys: 5.428 ± 0.348
5.481ThrLeu: 5.481 ± 0.361
1.497ThrMet: 1.497 ± 0.198
3.69ThrAsn: 3.69 ± 0.301
2.166ThrPro: 2.166 ± 0.241
1.925ThrGln: 1.925 ± 0.201
2.193ThrArg: 2.193 ± 0.258
4.091ThrSer: 4.091 ± 0.378
3.396ThrThr: 3.396 ± 0.453
4.84ThrVal: 4.84 ± 0.371
0.695ThrTrp: 0.695 ± 0.164
2.567ThrTyr: 2.567 ± 0.275
0.0ThrXaa: 0.0 ± 0.0
Val
3.556ValAla: 3.556 ± 0.471
0.588ValCys: 0.588 ± 0.152
4.572ValAsp: 4.572 ± 0.382
5.0ValGlu: 5.0 ± 0.481
2.914ValPhe: 2.914 ± 0.266
3.422ValGly: 3.422 ± 0.325
0.775ValHis: 0.775 ± 0.157
4.064ValIle: 4.064 ± 0.429
5.535ValLys: 5.535 ± 0.362
4.519ValLeu: 4.519 ± 0.348
1.471ValMet: 1.471 ± 0.233
3.877ValAsn: 3.877 ± 0.361
1.524ValPro: 1.524 ± 0.179
2.193ValGln: 2.193 ± 0.27
2.112ValArg: 2.112 ± 0.233
4.011ValSer: 4.011 ± 0.367
4.305ValThr: 4.305 ± 0.35
4.198ValVal: 4.198 ± 0.327
0.963ValTrp: 0.963 ± 0.172
2.834ValTyr: 2.834 ± 0.356
0.0ValXaa: 0.0 ± 0.0
Trp
0.481TrpAla: 0.481 ± 0.115
0.134TrpCys: 0.134 ± 0.061
0.722TrpAsp: 0.722 ± 0.119
0.829TrpGlu: 0.829 ± 0.178
0.455TrpPhe: 0.455 ± 0.118
0.535TrpGly: 0.535 ± 0.117
0.214TrpHis: 0.214 ± 0.073
0.829TrpIle: 0.829 ± 0.154
0.695TrpLys: 0.695 ± 0.125
0.909TrpLeu: 0.909 ± 0.166
0.508TrpMet: 0.508 ± 0.082
1.096TrpAsn: 1.096 ± 0.195
0.027TrpPro: 0.027 ± 0.029
0.321TrpGln: 0.321 ± 0.095
0.428TrpArg: 0.428 ± 0.104
0.695TrpSer: 0.695 ± 0.185
0.749TrpThr: 0.749 ± 0.148
0.615TrpVal: 0.615 ± 0.131
0.107TrpTrp: 0.107 ± 0.052
0.214TrpTyr: 0.214 ± 0.091
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.513TyrAla: 2.513 ± 0.252
0.348TyrCys: 0.348 ± 0.093
3.262TyrAsp: 3.262 ± 0.291
3.449TyrGlu: 3.449 ± 0.407
1.898TyrPhe: 1.898 ± 0.333
2.888TyrGly: 2.888 ± 0.32
0.722TyrHis: 0.722 ± 0.151
3.369TyrIle: 3.369 ± 0.379
3.93TyrLys: 3.93 ± 0.315
3.93TyrLeu: 3.93 ± 0.346
0.936TyrMet: 0.936 ± 0.153
2.62TyrAsn: 2.62 ± 0.268
1.203TyrPro: 1.203 ± 0.263
1.765TyrGln: 1.765 ± 0.182
1.658TyrArg: 1.658 ± 0.237
3.102TyrSer: 3.102 ± 0.283
3.235TyrThr: 3.235 ± 0.406
2.861TyrVal: 2.861 ± 0.282
0.267TyrTrp: 0.267 ± 0.08
2.032TyrTyr: 2.032 ± 0.225
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 182 proteins (37401 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski