Streptococcus phage Javan271

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6A5N7|A0A4D6A5N7_9CAUD Uncharacterized protein OS=Streptococcus phage Javan271 OX=2548086 GN=Javan271_0021 PE=4 SV=1
MM1 pKa = 7.53NDD3 pKa = 3.02KK4 pKa = 11.18GLFGTFDD11 pKa = 3.58YY12 pKa = 11.12DD13 pKa = 3.64RR14 pKa = 11.84DD15 pKa = 3.83VMQPPIEE22 pKa = 4.54KK23 pKa = 10.63EE24 pKa = 3.9EE25 pKa = 4.95LDD27 pKa = 5.25DD28 pKa = 3.46NHH30 pKa = 6.4YY31 pKa = 10.71WNGHH35 pKa = 4.05SWEE38 pKa = 4.7KK39 pKa = 11.22NDD41 pKa = 5.09DD42 pKa = 3.55

Molecular weight: 5.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6A6T4|A0A4D6A6T4_9CAUD HTH_17 domain-containing protein OS=Streptococcus phage Javan271 OX=2548086 GN=Javan271_0009 PE=4 SV=1
MM1 pKa = 7.69KK2 pKa = 9.39FTIKK6 pKa = 9.71GTEE9 pKa = 3.79KK10 pKa = 10.49LEE12 pKa = 3.91EE13 pKa = 3.97RR14 pKa = 11.84FRR16 pKa = 11.84AMNAIRR22 pKa = 11.84WDD24 pKa = 3.66GVVNKK29 pKa = 10.52SLTQMFNRR37 pKa = 11.84GARR40 pKa = 11.84PPGTPVGKK48 pKa = 7.82QTKK51 pKa = 8.21NHH53 pKa = 6.19SAGEE57 pKa = 4.07LRR59 pKa = 11.84RR60 pKa = 11.84SRR62 pKa = 11.84RR63 pKa = 11.84KK64 pKa = 9.85QEE66 pKa = 3.58ISSKK70 pKa = 10.22DD71 pKa = 3.32GSYY74 pKa = 9.97TGSFYY79 pKa = 9.74YY80 pKa = 10.33TKK82 pKa = 10.39DD83 pKa = 3.48YY84 pKa = 10.82APHH87 pKa = 6.32VEE89 pKa = 3.85YY90 pKa = 10.84GRR92 pKa = 11.84RR93 pKa = 11.84IVRR96 pKa = 11.84KK97 pKa = 8.37GKK99 pKa = 9.65QVGYY103 pKa = 11.11VNGKK107 pKa = 9.81KK108 pKa = 10.37YY109 pKa = 10.92LFNNVQKK116 pKa = 10.47QRR118 pKa = 11.84QIYY121 pKa = 8.74FKK123 pKa = 11.51DD124 pKa = 3.12MLAEE128 pKa = 3.99LRR130 pKa = 11.84KK131 pKa = 10.03KK132 pKa = 10.47RR133 pKa = 3.7

Molecular weight: 15.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

11607

42

964

187.2

21.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.272 ± 0.371

0.526 ± 0.098

6.186 ± 0.247

7.323 ± 0.506

3.946 ± 0.187

6.341 ± 0.409

1.378 ± 0.146

7.28 ± 0.287

8.917 ± 0.431

8.202 ± 0.346

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.438 ± 0.191

6.539 ± 0.24

2.447 ± 0.156

3.42 ± 0.171

3.55 ± 0.231

6.608 ± 0.334

7.047 ± 0.462

6.031 ± 0.252

1.439 ± 0.113

4.11 ± 0.213
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski