Roseibium sp. RKSG952

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Stappiaceae; Roseibium; unclassified Roseibium

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9448 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6N7ZS53|A0A6N7ZS53_9RHOB Phage portal protein OS=Roseibium sp. RKSG952 OX=2529384 GN=E1297_06985 PE=4 SV=1
MM1 pKa = 7.21HH2 pKa = 7.26VSTGLEE8 pKa = 3.8GLDD11 pKa = 3.69AKK13 pKa = 10.92LEE15 pKa = 4.26HH16 pKa = 6.85LSSEE20 pKa = 4.33DD21 pKa = 3.74LQLLLNFVAGNTLFTLYY38 pKa = 10.66HH39 pKa = 6.29EE40 pKa = 5.08AGHH43 pKa = 5.6MLISEE48 pKa = 4.93LGLPVLGQEE57 pKa = 4.22EE58 pKa = 4.38DD59 pKa = 3.78AVDD62 pKa = 3.71NLATVMMLSDD72 pKa = 4.42DD73 pKa = 4.1TRR75 pKa = 11.84DD76 pKa = 3.34MDD78 pKa = 4.21QLLSQAMIGWFLIASQNHH96 pKa = 6.77DD97 pKa = 3.35NLVFYY102 pKa = 10.95GEE104 pKa = 4.99HH105 pKa = 7.34DD106 pKa = 4.3LDD108 pKa = 3.95LQRR111 pKa = 11.84GYY113 pKa = 11.74QMLCLMVGADD123 pKa = 3.65PDD125 pKa = 3.94AFEE128 pKa = 6.02DD129 pKa = 4.42LANDD133 pKa = 3.94LDD135 pKa = 4.12LPEE138 pKa = 5.1DD139 pKa = 3.7RR140 pKa = 11.84RR141 pKa = 11.84EE142 pKa = 4.05TCAFDD147 pKa = 3.84YY148 pKa = 9.95EE149 pKa = 4.31QSADD153 pKa = 3.35SWDD156 pKa = 3.98LVTDD160 pKa = 4.14PFLRR164 pKa = 11.84PEE166 pKa = 3.86GHH168 pKa = 6.42RR169 pKa = 11.84GDD171 pKa = 5.16RR172 pKa = 11.84ITVAYY177 pKa = 8.45AQPEE181 pKa = 4.12PGQASMALFLQEE193 pKa = 4.24SEE195 pKa = 4.13LMEE198 pKa = 4.35MVAEE202 pKa = 4.76DD203 pKa = 4.97LDD205 pKa = 4.04GLYY208 pKa = 10.84ALPDD212 pKa = 4.4KK213 pKa = 10.58ITFQAMACGEE223 pKa = 4.22EE224 pKa = 3.88NAYY227 pKa = 9.45WDD229 pKa = 3.62PQARR233 pKa = 11.84EE234 pKa = 3.66MTLCYY239 pKa = 10.49EE240 pKa = 4.3LMAGFSEE247 pKa = 4.84IFLDD251 pKa = 5.43LLGTDD256 pKa = 3.87EE257 pKa = 4.43

Molecular weight: 28.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6N7ZVZ3|A0A6N7ZVZ3_9RHOB Glutamine--fructose-6-phosphate aminotransferase [isomerizing] OS=Roseibium sp. RKSG952 OX=2529384 GN=glmS PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.2QPSRR9 pKa = 11.84LVRR12 pKa = 11.84KK13 pKa = 9.21RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.48GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.93GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9448

0

9448

2960375

24

5708

313.3

34.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.041 ± 0.03

0.929 ± 0.009

6.119 ± 0.025

6.118 ± 0.022

3.943 ± 0.016

8.41 ± 0.03

2.068 ± 0.011

5.424 ± 0.019

3.66 ± 0.019

9.913 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.599 ± 0.013

3.003 ± 0.015

4.904 ± 0.017

3.348 ± 0.013

6.255 ± 0.029

5.805 ± 0.019

5.469 ± 0.019

7.23 ± 0.021

1.36 ± 0.01

2.4 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski