Streptococcus virus Sfi19

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Moineauvirus

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 43 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|O64288|O64288_9CAUD Orf116 gp OS=Streptococcus virus Sfi19 OX=72638 GN=orf116 PE=4 SV=1
MM1 pKa = 7.31SVSKK5 pKa = 9.71TSIMQTLNLDD15 pKa = 3.8EE16 pKa = 4.97TDD18 pKa = 3.78DD19 pKa = 3.8TALIPAYY26 pKa = 9.69IEE28 pKa = 4.01SAKK31 pKa = 10.51QYY33 pKa = 10.21IINAVGSDD41 pKa = 3.36SKK43 pKa = 11.26FYY45 pKa = 11.16DD46 pKa = 3.78LDD48 pKa = 3.69SVRR51 pKa = 11.84ALFDD55 pKa = 3.34TAVIALTSSYY65 pKa = 8.66FTYY68 pKa = 10.07RR69 pKa = 11.84VALTDD74 pKa = 3.37TATYY78 pKa = 9.41PVNLTLNSIIGQLRR92 pKa = 11.84GLYY95 pKa = 8.66ATYY98 pKa = 10.56SEE100 pKa = 4.46EE101 pKa = 5.06RR102 pKa = 11.84GDD104 pKa = 3.53

Molecular weight: 11.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9XJV2|Q9XJV2_9CAUD Orf229 gp OS=Streptococcus virus Sfi19 OX=72638 GN=orf229 PE=4 SV=1
MM1 pKa = 7.39SKK3 pKa = 10.47KK4 pKa = 10.48EE5 pKa = 3.7NWADD9 pKa = 3.44MLRR12 pKa = 11.84KK13 pKa = 9.8KK14 pKa = 8.54EE15 pKa = 4.35TYY17 pKa = 8.46HH18 pKa = 6.05TMVTHH23 pKa = 6.97GSMVTQGSMVTQGSVVTHH41 pKa = 6.34GSVITQGSMVTLRR54 pKa = 11.84SVVTQGSMVTHH65 pKa = 6.63GSMVTLRR72 pKa = 11.84SVITQGSMVTQGSMVAQGSMVTHH95 pKa = 6.56GSVVTHH101 pKa = 6.3GSMVTHH107 pKa = 6.69GSVITQGSMVTLRR120 pKa = 11.84SVVTLRR126 pKa = 11.84SRR128 pKa = 11.84NN129 pKa = 3.52

Molecular weight: 13.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

43

0

43

11145

51

1626

259.2

29.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.712 ± 0.609

0.655 ± 0.129

6.523 ± 0.287

6.371 ± 0.524

4.208 ± 0.224

7.124 ± 0.477

1.498 ± 0.133

6.514 ± 0.294

8.111 ± 0.486

7.528 ± 0.356

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.53 ± 0.188

6.308 ± 0.271

2.979 ± 0.222

4.127 ± 0.271

4.118 ± 0.283

6.218 ± 0.314

6.155 ± 0.34

6.335 ± 0.249

1.669 ± 0.141

4.316 ± 0.308
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski