Amino acid dipepetide frequency for Clostridium cylindrosporum DSM 605

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.044AlaAla: 4.044 ± 0.099	0.599AlaCys: 0.599 ± 0.028	2.59AlaAsp: 2.59 ± 0.063	3.479AlaGlu: 3.479 ± 0.082	2.383AlaPhe: 2.383 ± 0.072	3.744AlaGly: 3.744 ± 0.086	0.822AlaHis: 0.822 ± 0.031	5.891AlaIle: 5.891 ± 0.096	4.718AlaLys: 4.718 ± 0.091	5.9AlaLeu: 5.9 ± 0.094	1.618AlaMet: 1.618 ± 0.048	2.655AlaAsn: 2.655 ± 0.06	1.461AlaPro: 1.461 ± 0.046	1.348AlaGln: 1.348 ± 0.046	2.16AlaArg: 2.16 ± 0.057	4.045AlaSer: 4.045 ± 0.077	3.023AlaThr: 3.023 ± 0.073	3.959AlaVal: 3.959 ± 0.083	0.309AlaTrp: 0.309 ± 0.015	2.084AlaTyr: 2.084 ± 0.054	0.0AlaXaa: 0.0 ± 0.0
Cys	0.555CysAla: 0.555 ± 0.029	0.177CysCys: 0.177 ± 0.016	0.708CysAsp: 0.708 ± 0.034	0.753CysGlu: 0.753 ± 0.028	0.427CysPhe: 0.427 ± 0.025	1.001CysGly: 1.001 ± 0.04	0.187CysHis: 0.187 ± 0.016	1.074CysIle: 1.074 ± 0.047	0.868CysLys: 0.868 ± 0.028	0.854CysLeu: 0.854 ± 0.033	0.212CysMet: 0.212 ± 0.015	0.647CysAsn: 0.647 ± 0.028	0.447CysPro: 0.447 ± 0.028	0.191CysGln: 0.191 ± 0.014	0.435CysArg: 0.435 ± 0.028	0.839CysSer: 0.839 ± 0.034	0.595CysThr: 0.595 ± 0.028	0.576CysVal: 0.576 ± 0.024	0.058CysTrp: 0.058 ± 0.01	0.393CysTyr: 0.393 ± 0.022	0.0CysXaa: 0.0 ± 0.0
Asp	2.897AspAla: 2.897 ± 0.067	0.517AspCys: 0.517 ± 0.026	2.678AspAsp: 2.678 ± 0.069	4.489AspGlu: 4.489 ± 0.087	2.823AspPhe: 2.823 ± 0.066	3.266AspGly: 3.266 ± 0.071	0.628AspHis: 0.628 ± 0.034	6.784AspIle: 6.784 ± 0.102	5.367AspLys: 5.367 ± 0.097	4.584AspLeu: 4.584 ± 0.081	1.529AspMet: 1.529 ± 0.04	3.185AspAsn: 3.185 ± 0.065	1.362AspPro: 1.362 ± 0.044	0.782AspGln: 0.782 ± 0.037	2.042AspArg: 2.042 ± 0.071	3.628AspSer: 3.628 ± 0.061	2.647AspThr: 2.647 ± 0.058	3.968AspVal: 3.968 ± 0.079	0.285AspTrp: 0.285 ± 0.02	2.584AspTyr: 2.584 ± 0.06	0.0AspXaa: 0.0 ± 0.0
Glu	4.629GluAla: 4.629 ± 0.084	0.739GluCys: 0.739 ± 0.034	4.47GluAsp: 4.47 ± 0.074	6.783GluGlu: 6.783 ± 0.128	3.205GluPhe: 3.205 ± 0.07	4.544GluGly: 4.544 ± 0.079	0.927GluHis: 0.927 ± 0.039	6.956GluIle: 6.956 ± 0.1	7.38GluLys: 7.38 ± 0.106	5.958GluLeu: 5.958 ± 0.088	1.88GluMet: 1.88 ± 0.05	4.881GluAsn: 4.881 ± 0.077	1.287GluPro: 1.287 ± 0.04	1.387GluGln: 1.387 ± 0.047	2.761GluArg: 2.761 ± 0.065	4.362GluSer: 4.362 ± 0.086	3.039GluThr: 3.039 ± 0.064	5.763GluVal: 5.763 ± 0.086	0.393GluTrp: 0.393 ± 0.024	3.044GluTyr: 3.044 ± 0.077	0.0GluXaa: 0.0 ± 0.0
Phe	2.19PheAla: 2.19 ± 0.064	0.451PheCys: 0.451 ± 0.023	2.415PheAsp: 2.415 ± 0.053	2.782PheGlu: 2.782 ± 0.061	1.718PhePhe: 1.718 ± 0.053	2.814PheGly: 2.814 ± 0.059	0.541PheHis: 0.541 ± 0.029	4.437PheIle: 4.437 ± 0.093	3.766PheLys: 3.766 ± 0.069	3.659PheLeu: 3.659 ± 0.071	1.144PheMet: 1.144 ± 0.043	2.762PheAsn: 2.762 ± 0.061	1.158PhePro: 1.158 ± 0.039	0.929PheGln: 0.929 ± 0.041	1.301PheArg: 1.301 ± 0.041	3.322PheSer: 3.322 ± 0.07	2.354PheThr: 2.354 ± 0.049	2.711PheVal: 2.711 ± 0.061	0.238PheTrp: 0.238 ± 0.019	1.575PheTyr: 1.575 ± 0.049	0.0PheXaa: 0.0 ± 0.0
Gly	4.181GlyAla: 4.181 ± 0.093	0.916GlyCys: 0.916 ± 0.036	3.551GlyAsp: 3.551 ± 0.071	4.685GlyGlu: 4.685 ± 0.074	3.155GlyPhe: 3.155 ± 0.067	4.182GlyGly: 4.182 ± 0.09	1.131GlyHis: 1.131 ± 0.063	6.994GlyIle: 6.994 ± 0.105	5.538GlyLys: 5.538 ± 0.075	5.562GlyLeu: 5.562 ± 0.095	1.713GlyMet: 1.713 ± 0.052	3.023GlyAsn: 3.023 ± 0.061	1.319GlyPro: 1.319 ± 0.044	1.386GlyGln: 1.386 ± 0.043	2.435GlyArg: 2.435 ± 0.057	4.197GlySer: 4.197 ± 0.079	3.31GlyThr: 3.31 ± 0.072	5.154GlyVal: 5.154 ± 0.092	0.389GlyTrp: 0.389 ± 0.024	3.143GlyTyr: 3.143 ± 0.062	0.0GlyXaa: 0.0 ± 0.0
His	0.69HisAla: 0.69 ± 0.031	0.179HisCys: 0.179 ± 0.018	0.702HisAsp: 0.702 ± 0.039	0.922HisGlu: 0.922 ± 0.04	0.635HisPhe: 0.635 ± 0.027	1.128HisGly: 1.128 ± 0.061	0.308HisHis: 0.308 ± 0.042	1.406HisIle: 1.406 ± 0.041	1.041HisLys: 1.041 ± 0.042	1.177HisLeu: 1.177 ± 0.039	0.369HisMet: 0.369 ± 0.024	0.754HisAsn: 0.754 ± 0.033	0.613HisPro: 0.613 ± 0.03	0.332HisGln: 0.332 ± 0.026	0.612HisArg: 0.612 ± 0.028	0.957HisSer: 0.957 ± 0.038	0.704HisThr: 0.704 ± 0.027	0.777HisVal: 0.777 ± 0.033	0.086HisTrp: 0.086 ± 0.01	0.551HisTyr: 0.551 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	5.946IleAla: 5.946 ± 0.092	1.084IleCys: 1.084 ± 0.036	6.222IleAsp: 6.222 ± 0.098	7.619IleGlu: 7.619 ± 0.111	3.829IlePhe: 3.829 ± 0.091	6.47IleGly: 6.47 ± 0.111	1.247IleHis: 1.247 ± 0.044	9.252IleIle: 9.252 ± 0.157	8.818IleLys: 8.818 ± 0.139	9.005IleLeu: 9.005 ± 0.126	2.242IleMet: 2.242 ± 0.054	6.091IleAsn: 6.091 ± 0.101	3.289IlePro: 3.289 ± 0.071	2.07IleGln: 2.07 ± 0.054	3.206IleArg: 3.206 ± 0.062	7.595IleSer: 7.595 ± 0.105	5.032IleThr: 5.032 ± 0.073	6.456IleVal: 6.456 ± 0.106	0.475IleTrp: 0.475 ± 0.03	3.516IleTyr: 3.516 ± 0.075	0.0IleXaa: 0.0 ± 0.0
Lys	4.89LysAla: 4.89 ± 0.084	0.927LysCys: 0.927 ± 0.04	5.786LysAsp: 5.786 ± 0.094	8.089LysGlu: 8.089 ± 0.123	2.968LysPhe: 2.968 ± 0.065	5.885LysGly: 5.885 ± 0.098	1.169LysHis: 1.169 ± 0.045	7.818LysIle: 7.818 ± 0.107	7.941LysLys: 7.941 ± 0.121	7.283LysLeu: 7.283 ± 0.111	2.348LysMet: 2.348 ± 0.047	5.429LysAsn: 5.429 ± 0.095	2.087LysPro: 2.087 ± 0.059	1.808LysGln: 1.808 ± 0.053	3.252LysArg: 3.252 ± 0.06	5.906LysSer: 5.906 ± 0.09	4.062LysThr: 4.062 ± 0.072	6.763LysVal: 6.763 ± 0.113	0.551LysTrp: 0.551 ± 0.027	3.682LysTyr: 3.682 ± 0.08	0.0LysXaa: 0.0 ± 0.0
Leu	4.979LeuAla: 4.979 ± 0.078	1.011LeuCys: 1.011 ± 0.033	5.245LeuAsp: 5.245 ± 0.081	6.376LeuGlu: 6.376 ± 0.092	3.498LeuPhe: 3.498 ± 0.09	6.162LeuGly: 6.162 ± 0.105	1.155LeuHis: 1.155 ± 0.037	8.08LeuIle: 8.08 ± 0.124	8.424LeuLys: 8.424 ± 0.11	7.755LeuLeu: 7.755 ± 0.126	2.052LeuMet: 2.052 ± 0.045	5.462LeuAsn: 5.462 ± 0.089	2.614LeuPro: 2.614 ± 0.062	1.937LeuGln: 1.937 ± 0.05	3.299LeuArg: 3.299 ± 0.06	7.525LeuSer: 7.525 ± 0.099	4.562LeuThr: 4.562 ± 0.078	5.597LeuVal: 5.597 ± 0.093	0.497LeuTrp: 0.497 ± 0.026	3.057LeuTyr: 3.057 ± 0.064	0.0LeuXaa: 0.0 ± 0.0
Met	1.722MetAla: 1.722 ± 0.048	0.237MetCys: 0.237 ± 0.021	1.482MetAsp: 1.482 ± 0.04	1.742MetGlu: 1.742 ± 0.049	1.014MetPhe: 1.014 ± 0.032	1.667MetGly: 1.667 ± 0.043	0.356MetHis: 0.356 ± 0.022	2.236MetIle: 2.236 ± 0.051	2.38MetLys: 2.38 ± 0.052	2.32MetLeu: 2.32 ± 0.059	0.618MetMet: 0.618 ± 0.028	1.519MetAsn: 1.519 ± 0.042	0.856MetPro: 0.856 ± 0.034	0.659MetGln: 0.659 ± 0.031	0.803MetArg: 0.803 ± 0.031	1.705MetSer: 1.705 ± 0.045	1.122MetThr: 1.122 ± 0.036	1.622MetVal: 1.622 ± 0.047	0.148MetTrp: 0.148 ± 0.014	0.877MetTyr: 0.877 ± 0.033	0.0MetXaa: 0.0 ± 0.0
Asn	2.772AsnAla: 2.772 ± 0.062	0.614AsnCys: 0.614 ± 0.029	2.738AsnAsp: 2.738 ± 0.055	3.924AsnGlu: 3.924 ± 0.083	2.399AsnPhe: 2.399 ± 0.052	3.381AsnGly: 3.381 ± 0.079	0.76AsnHis: 0.76 ± 0.035	6.735AsnIle: 6.735 ± 0.096	5.392AsnLys: 5.392 ± 0.094	5.458AsnLeu: 5.458 ± 0.101	1.605AsnMet: 1.605 ± 0.04	3.535AsnAsn: 3.535 ± 0.078	2.107AsnPro: 2.107 ± 0.057	1.286AsnGln: 1.286 ± 0.047	1.966AsnArg: 1.966 ± 0.054	3.849AsnSer: 3.849 ± 0.072	2.838AsnThr: 2.838 ± 0.065	3.532AsnVal: 3.532 ± 0.072	0.317AsnTrp: 0.317 ± 0.023	2.35AsnTyr: 2.35 ± 0.063	0.0AsnXaa: 0.0 ± 0.0
Pro	1.385ProAla: 1.385 ± 0.047	0.324ProCys: 0.324 ± 0.022	1.37ProAsp: 1.37 ± 0.044	2.087ProGlu: 2.087 ± 0.055	1.359ProPhe: 1.359 ± 0.044	1.837ProGly: 1.837 ± 0.055	0.508ProHis: 0.508 ± 0.027	2.849ProIle: 2.849 ± 0.063	2.241ProLys: 2.241 ± 0.055	2.553ProLeu: 2.553 ± 0.064	0.696ProMet: 0.696 ± 0.031	1.484ProAsn: 1.484 ± 0.048	0.683ProPro: 0.683 ± 0.028	0.656ProGln: 0.656 ± 0.026	0.991ProArg: 0.991 ± 0.039	2.137ProSer: 2.137 ± 0.055	1.548ProThr: 1.548 ± 0.043	1.983ProVal: 1.983 ± 0.055	0.231ProTrp: 0.231 ± 0.016	1.191ProTyr: 1.191 ± 0.037	0.0ProXaa: 0.0 ± 0.0
Gln	1.211GlnAla: 1.211 ± 0.044	0.219GlnCys: 0.219 ± 0.017	1.103GlnAsp: 1.103 ± 0.034	1.633GlnGlu: 1.633 ± 0.048	0.829GlnPhe: 0.829 ± 0.034	1.676GlnGly: 1.676 ± 0.057	0.313GlnHis: 0.313 ± 0.019	1.929GlnIle: 1.929 ± 0.059	1.755GlnLys: 1.755 ± 0.054	1.796GlnLeu: 1.796 ± 0.049	0.595GlnMet: 0.595 ± 0.029	1.223GlnAsn: 1.223 ± 0.044	0.492GlnPro: 0.492 ± 0.026	0.592GlnGln: 0.592 ± 0.029	1.116GlnArg: 1.116 ± 0.038	1.41GlnSer: 1.41 ± 0.056	0.943GlnThr: 0.943 ± 0.035	1.487GlnVal: 1.487 ± 0.043	0.204GlnTrp: 0.204 ± 0.019	0.768GlnTyr: 0.768 ± 0.029	0.0GlnXaa: 0.0 ± 0.0
Arg	2.005ArgAla: 2.005 ± 0.051	0.422ArgCys: 0.422 ± 0.027	2.287ArgAsp: 2.287 ± 0.069	3.375ArgGlu: 3.375 ± 0.066	1.608ArgPhe: 1.608 ± 0.048	2.383ArgGly: 2.383 ± 0.067	0.526ArgHis: 0.526 ± 0.027	3.279ArgIle: 3.279 ± 0.073	3.004ArgLys: 3.004 ± 0.058	3.172ArgLeu: 3.172 ± 0.063	0.851ArgMet: 0.851 ± 0.034	1.94ArgAsn: 1.94 ± 0.055	0.893ArgPro: 0.893 ± 0.034	0.93ArgGln: 0.93 ± 0.031	1.551ArgArg: 1.551 ± 0.053	1.942ArgSer: 1.942 ± 0.052	1.491ArgThr: 1.491 ± 0.046	2.648ArgVal: 2.648 ± 0.055	0.251ArgTrp: 0.251 ± 0.021	1.468ArgTyr: 1.468 ± 0.045	0.0ArgXaa: 0.0 ± 0.0
Ser	3.484SerAla: 3.484 ± 0.07	0.74SerCys: 0.74 ± 0.036	3.559SerAsp: 3.559 ± 0.079	4.628SerGlu: 4.628 ± 0.08	3.22SerPhe: 3.22 ± 0.059	4.713SerGly: 4.713 ± 0.08	1.022SerHis: 1.022 ± 0.036	7.681SerIle: 7.681 ± 0.117	6.317SerLys: 6.317 ± 0.095	7.145SerLeu: 7.145 ± 0.093	1.72SerMet: 1.72 ± 0.045	3.921SerAsn: 3.921 ± 0.076	2.027SerPro: 2.027 ± 0.057	1.624SerGln: 1.624 ± 0.051	2.527SerArg: 2.527 ± 0.063	5.559SerSer: 5.559 ± 0.127	3.613SerThr: 3.613 ± 0.076	4.321SerVal: 4.321 ± 0.08	0.393SerTrp: 0.393 ± 0.025	2.719SerTyr: 2.719 ± 0.063	0.0SerXaa: 0.0 ± 0.0
Thr	2.854ThrAla: 2.854 ± 0.073	0.499ThrCys: 0.499 ± 0.026	2.382ThrAsp: 2.382 ± 0.055	2.862ThrGlu: 2.862 ± 0.07	2.113ThrPhe: 2.113 ± 0.052	3.835ThrGly: 3.835 ± 0.066	0.74ThrHis: 0.74 ± 0.033	4.885ThrIle: 4.885 ± 0.073	3.784ThrLys: 3.784 ± 0.074	5.14ThrLeu: 5.14 ± 0.071	1.147ThrMet: 1.147 ± 0.037	2.325ThrAsn: 2.325 ± 0.054	2.046ThrPro: 2.046 ± 0.051	1.057ThrGln: 1.057 ± 0.035	1.679ThrArg: 1.679 ± 0.047	3.765ThrSer: 3.765 ± 0.069	2.725ThrThr: 2.725 ± 0.074	3.45ThrVal: 3.45 ± 0.066	0.323ThrTrp: 0.323 ± 0.021	1.875ThrTyr: 1.875 ± 0.052	0.0ThrXaa: 0.0 ± 0.0
Val	4.257ValAla: 4.257 ± 0.101	0.874ValCys: 0.874 ± 0.033	4.223ValAsp: 4.223 ± 0.08	5.07ValGlu: 5.07 ± 0.084	3.014ValPhe: 3.014 ± 0.066	4.364ValGly: 4.364 ± 0.086	0.935ValHis: 0.935 ± 0.04	6.524ValIle: 6.524 ± 0.102	5.777ValLys: 5.777 ± 0.106	6.095ValLeu: 6.095 ± 0.09	1.611ValMet: 1.611 ± 0.046	3.863ValAsn: 3.863 ± 0.067	1.989ValPro: 1.989 ± 0.054	1.411ValGln: 1.411 ± 0.046	2.213ValArg: 2.213 ± 0.052	4.905ValSer: 4.905 ± 0.094	3.481ValThr: 3.481 ± 0.077	5.159ValVal: 5.159 ± 0.101	0.343ValTrp: 0.343 ± 0.02	2.543ValTyr: 2.543 ± 0.053	0.0ValXaa: 0.0 ± 0.0
Trp	0.281TrpAla: 0.281 ± 0.02	0.07TrpCys: 0.07 ± 0.01	0.336TrpAsp: 0.336 ± 0.024	0.379TrpGlu: 0.379 ± 0.024	0.269TrpPhe: 0.269 ± 0.02	0.436TrpGly: 0.436 ± 0.024	0.134TrpHis: 0.134 ± 0.014	0.554TrpIle: 0.554 ± 0.029	0.431TrpLys: 0.431 ± 0.025	0.426TrpLeu: 0.426 ± 0.022	0.177TrpMet: 0.177 ± 0.016	0.389TrpAsn: 0.389 ± 0.025	0.13TrpPro: 0.13 ± 0.013	0.17TrpGln: 0.17 ± 0.015	0.241TrpArg: 0.241 ± 0.018	0.391TrpSer: 0.391 ± 0.026	0.26TrpThr: 0.26 ± 0.019	0.402TrpVal: 0.402 ± 0.025	0.065TrpTrp: 0.065 ± 0.01	0.234TrpTyr: 0.234 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.859TyrAla: 1.859 ± 0.052	0.398TyrCys: 0.398 ± 0.022	2.201TyrAsp: 2.201 ± 0.047	2.714TyrGlu: 2.714 ± 0.07	1.802TyrPhe: 1.802 ± 0.05	2.476TyrGly: 2.476 ± 0.061	0.531TyrHis: 0.531 ± 0.028	4.097TyrIle: 4.097 ± 0.088	3.703TyrLys: 3.703 ± 0.081	3.446TyrLeu: 3.446 ± 0.074	0.957TyrMet: 0.957 ± 0.032	2.473TyrAsn: 2.473 ± 0.05	1.252TyrPro: 1.252 ± 0.038	0.74TyrGln: 0.74 ± 0.034	1.435TyrArg: 1.435 ± 0.046	2.894TyrSer: 2.894 ± 0.073	2.135TyrThr: 2.135 ± 0.053	2.326TyrVal: 2.326 ± 0.054	0.218TyrTrp: 0.218 ± 0.017	1.601TyrTyr: 1.601 ± 0.056	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2621 proteins (789336 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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