Bos taurus papillomavirus 12
Average proteome isoelectric point is 5.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G1CR70|G1CR70_9PAPI Replication protein E1 OS=Bos taurus papillomavirus 12 OX=1070324 GN=E1 PE=3 SV=1
MM1 pKa = 7.82 RR2 pKa = 11.84 GSQPAISDD10 pKa = 3.1 ISEE13 pKa = 4.0 EE14 pKa = 4.12 LQEE17 pKa = 4.37 LVCPVNLEE25 pKa = 4.07 CDD27 pKa = 3.41 EE28 pKa = 4.31 EE29 pKa = 4.15 LAPEE33 pKa = 4.3 NDD35 pKa = 3.58 DD36 pKa = 4.04 PYY38 pKa = 11.56 AVAVACHH45 pKa = 6.0 KK46 pKa = 10.58 CGNQVAFCVYY56 pKa = 10.47 APRR59 pKa = 11.84 EE60 pKa = 4.38 GIRR63 pKa = 11.84 QFQQLLLDD71 pKa = 5.2 CLDD74 pKa = 4.43 FCCPACSLNLLQRR87 pKa = 11.84 NGLL90 pKa = 3.69
Molecular weight: 9.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.053
IPC2_protein 4.177
IPC_protein 4.05
Toseland 3.872
ProMoST 4.19
Dawson 4.012
Bjellqvist 4.177
Wikipedia 3.91
Rodwell 3.884
Grimsley 3.795
Solomon 3.999
Lehninger 3.961
Nozaki 4.139
DTASelect 4.279
Thurlkill 3.91
EMBOSS 3.923
Sillero 4.164
Patrickios 0.146
IPC_peptide 3.999
IPC2_peptide 4.151
IPC2.peptide.svr19 4.089
Protein with the highest isoelectric point:
>tr|G1CR72|G1CR72_9PAPI E4 protein OS=Bos taurus papillomavirus 12 OX=1070324 GN=E4 PE=4 SV=1
MM1 pKa = 8.04 DD2 pKa = 5.48 PLAARR7 pKa = 11.84 FDD9 pKa = 3.95 ALQEE13 pKa = 3.89 EE14 pKa = 5.03 LMQIYY19 pKa = 10.06 EE20 pKa = 4.29 RR21 pKa = 11.84 GEE23 pKa = 4.18 STLDD27 pKa = 3.01 AQIRR31 pKa = 11.84 HH32 pKa = 5.8 WEE34 pKa = 4.42 LIRR37 pKa = 11.84 HH38 pKa = 5.18 EE39 pKa = 4.08 QATYY43 pKa = 9.83 FVARR47 pKa = 11.84 RR48 pKa = 11.84 QGITRR53 pKa = 11.84 LGLYY57 pKa = 7.23 PVPSASVSEE66 pKa = 4.28 ARR68 pKa = 11.84 AKK70 pKa = 10.02 QAIEE74 pKa = 3.75 MHH76 pKa = 6.88 LLLQSLKK83 pKa = 10.15 KK84 pKa = 10.13 SAYY87 pKa = 9.86 ADD89 pKa = 3.68 EE90 pKa = 4.63 RR91 pKa = 11.84 WTLVEE96 pKa = 3.87 TSFEE100 pKa = 4.41 HH101 pKa = 6.78 FNSPPARR108 pKa = 11.84 TLKK111 pKa = 10.55 KK112 pKa = 10.49 GPTQVTVIYY121 pKa = 10.62 DD122 pKa = 3.89 NNPEE126 pKa = 3.86 NAMTYY131 pKa = 7.21 TLWKK135 pKa = 9.07 YY136 pKa = 11.23 VYY138 pKa = 10.63 VLDD141 pKa = 5.73 PNDD144 pKa = 3.32 VWHH147 pKa = 7.05 KK148 pKa = 10.66 LPSDD152 pKa = 3.0 VDD154 pKa = 3.82 YY155 pKa = 11.77 YY156 pKa = 11.33 GIYY159 pKa = 8.9 YY160 pKa = 9.65 TDD162 pKa = 4.66 LDD164 pKa = 3.86 QQRR167 pKa = 11.84 VYY169 pKa = 10.64 YY170 pKa = 10.77 VSFGTDD176 pKa = 2.73 AEE178 pKa = 4.53 QYY180 pKa = 8.91 TGSGQWTVHH189 pKa = 5.92 FANKK193 pKa = 8.69 TFSAPVTSSAGGHH206 pKa = 5.9 SGLEE210 pKa = 4.15 EE211 pKa = 3.95 EE212 pKa = 4.73 QAQGPRR218 pKa = 11.84 QLAARR223 pKa = 11.84 RR224 pKa = 11.84 QATYY228 pKa = 9.74 RR229 pKa = 11.84 GRR231 pKa = 11.84 RR232 pKa = 11.84 SRR234 pKa = 11.84 RR235 pKa = 11.84 SRR237 pKa = 11.84 TPQTPPRR244 pKa = 11.84 SRR246 pKa = 11.84 SRR248 pKa = 11.84 SEE250 pKa = 3.63 SRR252 pKa = 11.84 SRR254 pKa = 11.84 SASSSSDD261 pKa = 2.85 TDD263 pKa = 2.95 HH264 pKa = 7.78 RR265 pKa = 11.84 SRR267 pKa = 11.84 SRR269 pKa = 11.84 QRR271 pKa = 11.84 NGGRR275 pKa = 11.84 GFRR278 pKa = 11.84 RR279 pKa = 11.84 GDD281 pKa = 3.24 STGEE285 pKa = 3.87 HH286 pKa = 6.89 PEE288 pKa = 3.96 SEE290 pKa = 4.6 EE291 pKa = 3.53 EE292 pKa = 4.22 SGRR295 pKa = 11.84 HH296 pKa = 4.87 SPGPPGSLGRR306 pKa = 11.84 RR307 pKa = 11.84 TAEE310 pKa = 3.69 TSGASGRR317 pKa = 11.84 RR318 pKa = 11.84 RR319 pKa = 11.84 TPRR322 pKa = 11.84 VVQLLKK328 pKa = 10.83 DD329 pKa = 3.52 AADD332 pKa = 3.95 PPVLLLRR339 pKa = 11.84 GPANTLKK346 pKa = 10.7 GFRR349 pKa = 11.84 RR350 pKa = 11.84 KK351 pKa = 9.81 ARR353 pKa = 11.84 LKK355 pKa = 8.39 YY356 pKa = 10.0 SEE358 pKa = 5.27 YY359 pKa = 9.32 YY360 pKa = 10.72 SCMSTAFSWVSGTCTEE376 pKa = 5.04 RR377 pKa = 11.84 LGTCQRR383 pKa = 11.84 MLVAFNSDD391 pKa = 3.23 SQRR394 pKa = 11.84 TRR396 pKa = 11.84 FLCNVHH402 pKa = 6.47 IPRR405 pKa = 11.84 SVTFVKK411 pKa = 10.85 GSFDD415 pKa = 3.5 ALL417 pKa = 3.54
Molecular weight: 47.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.86
IPC2_protein 9.297
IPC_protein 9.706
Toseland 9.663
ProMoST 9.604
Dawson 9.999
Bjellqvist 9.794
Wikipedia 10.262
Rodwell 10.101
Grimsley 10.116
Solomon 10.043
Lehninger 9.984
Nozaki 9.736
DTASelect 9.78
Thurlkill 9.809
EMBOSS 10.116
Sillero 9.926
Patrickios 5.016
IPC_peptide 10.028
IPC2_peptide 8.726
IPC2.peptide.svr19 8.228
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
2409
41
596
301.1
33.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.853 ± 0.684
1.868 ± 0.546
6.476 ± 0.649
6.185 ± 0.357
4.608 ± 0.491
6.434 ± 0.689
1.91 ± 0.184
4.4 ± 0.735
4.649 ± 0.969
9.174 ± 1.352
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.743 ± 0.225
4.525 ± 0.666
6.517 ± 0.8
4.774 ± 0.291
6.434 ± 1.078
7.057 ± 0.815
6.517 ± 0.787
6.31 ± 0.484
1.411 ± 0.341
3.155 ± 0.34
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here