Temperate fruit decay-associated virus
Average proteome isoelectric point is 8.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0K0TNV7|A0A0K0TNV7_9VIRU Viral protein RNase Z OS=Temperate fruit decay-associated virus OX=1628899 PE=4 SV=1
MM1 pKa = 7.7 APNLISRR8 pKa = 11.84 NFVFTINNPTFNPDD22 pKa = 3.33 DD23 pKa = 4.54 LFVGEE28 pKa = 3.99 PPEE31 pKa = 4.08 WVRR34 pKa = 11.84 CAIWQLEE41 pKa = 4.19 EE42 pKa = 4.87 GEE44 pKa = 4.68 QGTQHH49 pKa = 5.04 IQGYY53 pKa = 9.82 IEE55 pKa = 3.8 FTKK58 pKa = 10.57 AIRR61 pKa = 11.84 ASRR64 pKa = 11.84 FCQIINGDD72 pKa = 3.79 NPHH75 pKa = 7.09 LEE77 pKa = 4.04 VRR79 pKa = 11.84 RR80 pKa = 11.84 GSRR83 pKa = 11.84 DD84 pKa = 2.97 QAYY87 pKa = 9.56 NYY89 pKa = 9.71 CSKK92 pKa = 11.18 EE93 pKa = 3.95 EE94 pKa = 4.08 TRR96 pKa = 11.84 VSGPYY101 pKa = 9.62 TYY103 pKa = 10.99 GSWKK107 pKa = 9.26 LCKK110 pKa = 9.94 GRR112 pKa = 11.84 RR113 pKa = 11.84 TDD115 pKa = 3.95 IEE117 pKa = 4.12 AATTAILEE125 pKa = 3.98 QDD127 pKa = 3.45 ADD129 pKa = 4.43 DD130 pKa = 6.27 DD131 pKa = 4.4 EE132 pKa = 5.68 LLHH135 pKa = 5.77 NHH137 pKa = 6.24 SGVMAKK143 pKa = 10.12 YY144 pKa = 8.86 PKK146 pKa = 9.56 FVKK149 pKa = 10.43 FLRR152 pKa = 11.84 EE153 pKa = 3.66 HH154 pKa = 6.46 KK155 pKa = 9.81 RR156 pKa = 11.84 RR157 pKa = 11.84 KK158 pKa = 9.13 INSEE162 pKa = 3.4 IVFPHH167 pKa = 6.7 EE168 pKa = 3.56 LHH170 pKa = 6.66 PWQISLISYY179 pKa = 10.53 LEE181 pKa = 4.54 DD182 pKa = 3.31 EE183 pKa = 5.23 PIPRR187 pKa = 11.84 KK188 pKa = 9.86 IKK190 pKa = 9.68 WFVDD194 pKa = 3.25 EE195 pKa = 5.18 RR196 pKa = 11.84 GGSGKK201 pKa = 8.53 STFAKK206 pKa = 10.2 HH207 pKa = 5.01 MARR210 pKa = 11.84 HH211 pKa = 5.31 GAFYY215 pKa = 10.47 TRR217 pKa = 11.84 GGKK220 pKa = 7.86 TPDD223 pKa = 2.83 VAFAFRR229 pKa = 11.84 EE230 pKa = 4.15 HH231 pKa = 6.85 LSSNPHH237 pKa = 4.22 STIVIFDD244 pKa = 3.6 FTRR247 pKa = 11.84 SGKK250 pKa = 10.44 DD251 pKa = 3.16 YY252 pKa = 11.2 VNYY255 pKa = 10.49 DD256 pKa = 3.05 ILEE259 pKa = 4.18 QIKK262 pKa = 10.81 DD263 pKa = 3.96 GIVVSNKK270 pKa = 9.81 YY271 pKa = 10.16 EE272 pKa = 3.99 STTIISPVQHH282 pKa = 6.69 CIVFANWEE290 pKa = 4.21 PNKK293 pKa = 10.98 GEE295 pKa = 4.24 LVPGG299 pKa = 4.04
Molecular weight: 34.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.287
IPC2_protein 6.211
IPC_protein 6.338
Toseland 6.664
ProMoST 6.737
Dawson 6.693
Bjellqvist 6.634
Wikipedia 6.707
Rodwell 6.678
Grimsley 6.81
Solomon 6.693
Lehninger 6.693
Nozaki 6.956
DTASelect 7.132
Thurlkill 7.161
EMBOSS 7.146
Sillero 7.073
Patrickios 4.291
IPC_peptide 6.707
IPC2_peptide 6.912
IPC2.peptide.svr19 6.845
Protein with the highest isoelectric point:
>tr|A0A0H3VFP7|A0A0H3VFP7_9VIRU RNA-binding protein OS=Temperate fruit decay-associated virus OX=1628899 PE=4 SV=1
MM1 pKa = 8.02 AIRR4 pKa = 11.84 YY5 pKa = 5.88 PLKK8 pKa = 10.05 RR9 pKa = 11.84 RR10 pKa = 11.84 RR11 pKa = 11.84 PLKK14 pKa = 8.74 RR15 pKa = 11.84 RR16 pKa = 11.84 RR17 pKa = 11.84 RR18 pKa = 11.84 IFRR21 pKa = 11.84 RR22 pKa = 11.84 RR23 pKa = 11.84 KK24 pKa = 9.33 KK25 pKa = 10.12 RR26 pKa = 11.84 YY27 pKa = 6.82 STARR31 pKa = 11.84 RR32 pKa = 11.84 KK33 pKa = 10.23 YY34 pKa = 9.08 EE35 pKa = 3.74 APQYY39 pKa = 10.3 IRR41 pKa = 11.84 LYY43 pKa = 10.84 SNPLVINSGGSMVMQSVFPMGTGQTSRR70 pKa = 11.84 LSNRR74 pKa = 11.84 TAVTGIMVRR83 pKa = 11.84 VASAVMANLVTATLQEE99 pKa = 4.24 LVIHH103 pKa = 6.19 NWFILDD109 pKa = 4.0 KK110 pKa = 10.95 APTGNDD116 pKa = 2.69 IPGPSIIFDD125 pKa = 3.74 GVSPWNYY132 pKa = 10.11 SVEE135 pKa = 4.02 HH136 pKa = 6.43 DD137 pKa = 3.35 RR138 pKa = 11.84 KK139 pKa = 10.72 KK140 pKa = 10.72 RR141 pKa = 11.84 FSVLKK146 pKa = 9.98 KK147 pKa = 8.56 WHH149 pKa = 6.43 TKK151 pKa = 8.51 LTTGGTWTANSSCQVLPRR169 pKa = 11.84 SVTGKK174 pKa = 10.23 SFMWRR179 pKa = 11.84 PKK181 pKa = 10.6 KK182 pKa = 10.22 PVITYY187 pKa = 7.65 WKK189 pKa = 10.32 NNATANYY196 pKa = 10.28 SDD198 pKa = 4.15 VEE200 pKa = 4.71 SNALLWVMIVSSSIPQQTAANCTCNIYY227 pKa = 10.84 YY228 pKa = 10.77 DD229 pKa = 3.34 NTVYY233 pKa = 10.12 FKK235 pKa = 11.11 CLL237 pKa = 3.15
Molecular weight: 27.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.323
IPC2_protein 10.043
IPC_protein 10.833
Toseland 10.833
ProMoST 10.555
Dawson 10.935
Bjellqvist 10.657
Wikipedia 11.155
Rodwell 11.199
Grimsley 10.994
Solomon 11.038
Lehninger 11.008
Nozaki 10.804
DTASelect 10.657
Thurlkill 10.833
EMBOSS 11.228
Sillero 10.862
Patrickios 10.906
IPC_peptide 11.052
IPC2_peptide 9.706
IPC2.peptide.svr19 8.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
848
77
299
169.6
19.48
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.835 ± 0.561
1.415 ± 0.156
3.302 ± 0.852
4.245 ± 1.382
4.245 ± 0.692
5.542 ± 0.498
2.712 ± 0.563
8.255 ± 0.763
5.896 ± 0.416
9.788 ± 2.567
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.476 ± 0.674
5.307 ± 0.564
5.189 ± 0.409
3.656 ± 0.363
7.193 ± 0.791
8.726 ± 1.388
4.953 ± 1.027
6.132 ± 0.535
2.123 ± 0.277
4.009 ± 0.239
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here