Pepper vein yellows virus 5

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Polerovirus

Average proteome isoelectric point is 7.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I4Q1V0|A0A2I4Q1V0_9LUTE P0 OS=Pepper vein yellows virus 5 OX=1965355 PE=4 SV=1
MM1 pKa = 7.51EE2 pKa = 4.85MVDD5 pKa = 3.36HH6 pKa = 6.88ATPAVADD13 pKa = 3.51AHH15 pKa = 7.05DD16 pKa = 4.36RR17 pKa = 11.84FALSLWSHH25 pKa = 7.07PLGAHH30 pKa = 5.94GGEE33 pKa = 4.23IEE35 pKa = 4.49DD36 pKa = 5.3DD37 pKa = 3.54EE38 pKa = 4.7MEE40 pKa = 4.97AGTEE44 pKa = 3.77EE45 pKa = 4.87AEE47 pKa = 4.59IEE49 pKa = 4.28LEE51 pKa = 4.3DD52 pKa = 3.73GRR54 pKa = 11.84ATARR58 pKa = 11.84LSSSTRR64 pKa = 11.84TQSRR68 pKa = 11.84IVPQEE73 pKa = 3.74LSPSGRR79 pKa = 11.84LYY81 pKa = 10.52QRR83 pKa = 11.84ASRR86 pKa = 11.84FQVEE90 pKa = 4.21FLKK93 pKa = 9.53PTMNIRR99 pKa = 11.84SQWSTYY105 pKa = 10.07ASSVNPLPQRR115 pKa = 11.84RR116 pKa = 11.84APSLTSWTPTASLLVSNPPCASSPSPKK143 pKa = 9.88AGKK146 pKa = 9.89RR147 pKa = 11.84LLGLRR152 pKa = 11.84RR153 pKa = 11.84LTGG156 pKa = 3.3

Molecular weight: 17.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I4Q1J5|A0A2I4Q1J5_9LUTE Movement protein OS=Pepper vein yellows virus 5 OX=1965355 PE=3 SV=1
MM1 pKa = 6.77NTGGVRR7 pKa = 11.84SDD9 pKa = 3.46NNGNGGSRR17 pKa = 11.84NTRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84RR24 pKa = 11.84PRR26 pKa = 11.84QVRR29 pKa = 11.84PVVVVAPPGRR39 pKa = 11.84ARR41 pKa = 11.84RR42 pKa = 11.84GNRR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84NGGRR52 pKa = 11.84NRR54 pKa = 11.84RR55 pKa = 11.84SRR57 pKa = 11.84DD58 pKa = 3.15RR59 pKa = 11.84VGGRR63 pKa = 11.84SSNSEE68 pKa = 3.6TFVFNKK74 pKa = 10.46DD75 pKa = 3.56SIKK78 pKa = 10.74DD79 pKa = 3.67SSSGAVTFGPSLSEE93 pKa = 4.37SIALSGGVLKK103 pKa = 10.54AYY105 pKa = 10.22HH106 pKa = 6.68EE107 pKa = 4.5YY108 pKa = 10.51KK109 pKa = 9.32ITMVNIRR116 pKa = 11.84FVSEE120 pKa = 4.1SSSTAEE126 pKa = 3.6GSIAYY131 pKa = 9.39EE132 pKa = 4.08LDD134 pKa = 3.39PHH136 pKa = 7.02CKK138 pKa = 8.88LTSLQSTLRR147 pKa = 11.84KK148 pKa = 9.77FPVTKK153 pKa = 10.36GGQATFRR160 pKa = 11.84ASQINGVEE168 pKa = 3.87WHH170 pKa = 6.98DD171 pKa = 3.52TSEE174 pKa = 4.07DD175 pKa = 3.6QFRR178 pKa = 11.84LLYY181 pKa = 10.27KK182 pKa = 11.03GNGTKK187 pKa = 10.28NVAAGFFQIRR197 pKa = 11.84FTVQLHH203 pKa = 5.06NPKK206 pKa = 10.54

Molecular weight: 22.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

3126

45

1085

446.6

49.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.718 ± 0.406

1.44 ± 0.265

3.775 ± 0.641

6.686 ± 0.516

4.319 ± 0.454

7.518 ± 0.403

2.143 ± 0.282

4.031 ± 0.423

5.598 ± 0.475

8.221 ± 1.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.855 ± 0.101

4.191 ± 0.459

6.558 ± 0.542

3.679 ± 0.358

6.75 ± 0.96

9.629 ± 0.411

6.078 ± 0.483

6.366 ± 0.518

1.887 ± 0.243

2.527 ± 0.329
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski