Streptococcus phage Javan246

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AV75|A0A4D6AV75_9CAUD Major tail shaft protein OS=Streptococcus phage Javan246 OX=2548068 GN=Javan246_0012 PE=4 SV=1
MM1 pKa = 7.52SVQQSIVNGFTSRR14 pKa = 11.84RR15 pKa = 11.84GLITYY20 pKa = 10.89SMLGSRR26 pKa = 11.84NGSDD30 pKa = 3.22GTGDD34 pKa = 3.3CSGIMSQVLKK44 pKa = 10.46EE45 pKa = 4.07AGIKK49 pKa = 10.09IIGLPSTVTLGQQLANNSFYY69 pKa = 10.69RR70 pKa = 11.84ISINQDD76 pKa = 1.68WDD78 pKa = 3.44AQTGDD83 pKa = 3.49IVLISWGADD92 pKa = 2.74MSTSGGAGGHH102 pKa = 5.4VGAMLDD108 pKa = 3.46SVNFISCDD116 pKa = 3.34YY117 pKa = 8.99STQGAPGQAINTYY130 pKa = 9.29PWDD133 pKa = 3.56YY134 pKa = 11.01YY135 pKa = 11.75YY136 pKa = 11.3NANNPTYY143 pKa = 10.46IEE145 pKa = 3.8VWRR148 pKa = 11.84YY149 pKa = 9.56NGNAPEE155 pKa = 4.38KK156 pKa = 9.19PLPNTAVAPSSSRR169 pKa = 11.84KK170 pKa = 8.96PSSKK174 pKa = 10.55AYY176 pKa = 10.32YY177 pKa = 9.55LANDD181 pKa = 3.48VQLVNGIYY189 pKa = 9.81QIKK192 pKa = 9.99CDD194 pKa = 3.96YY195 pKa = 9.68LCPVGFDD202 pKa = 3.08WTEE205 pKa = 3.5NGIPVSLVNWVDD217 pKa = 3.35EE218 pKa = 4.65NGNHH222 pKa = 5.92VPDD225 pKa = 5.84GEE227 pKa = 4.74DD228 pKa = 3.19KK229 pKa = 11.05DD230 pKa = 5.35FKK232 pKa = 11.21AGMYY236 pKa = 10.18FSFEE240 pKa = 3.82VDD242 pKa = 3.24EE243 pKa = 4.19VHH245 pKa = 6.66ITDD248 pKa = 4.07TGDD251 pKa = 2.6GGYY254 pKa = 10.72YY255 pKa = 9.71GGYY258 pKa = 8.98YY259 pKa = 9.64YY260 pKa = 10.9RR261 pKa = 11.84SFEE264 pKa = 3.95FGQFGTVWLSVWDD277 pKa = 4.1KK278 pKa = 11.71DD279 pKa = 5.27DD280 pKa = 4.24LVNYY284 pKa = 9.56YY285 pKa = 10.4NN286 pKa = 3.92

Molecular weight: 31.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AVB8|A0A4D6AVB8_9CAUD Antirepressor protein OS=Streptococcus phage Javan246 OX=2548068 GN=Javan246_0048 PE=4 SV=1
MM1 pKa = 7.8AKK3 pKa = 8.42NTKK6 pKa = 7.57QTSAKK11 pKa = 9.98VATKK15 pKa = 10.32ASKK18 pKa = 10.54ALRR21 pKa = 11.84DD22 pKa = 3.82GRR24 pKa = 11.84SSARR28 pKa = 11.84TKK30 pKa = 10.73SIAGSALSQTRR41 pKa = 11.84KK42 pKa = 9.83KK43 pKa = 10.82

Molecular weight: 4.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

11178

43

1297

192.7

21.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.443 ± 0.687

0.528 ± 0.088

6.361 ± 0.239

7.837 ± 0.36

3.793 ± 0.209

6.477 ± 0.575

1.279 ± 0.16

6.96 ± 0.312

8.445 ± 0.367

8.213 ± 0.241

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.263 ± 0.142

5.6 ± 0.186

2.738 ± 0.222

4.232 ± 0.269

4.402 ± 0.229

6.754 ± 0.407

5.734 ± 0.385

6.146 ± 0.288

1.002 ± 0.102

3.793 ± 0.316
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski