Peniophora sp. CBMAI 1063 
Average proteome isoelectric point is 6.43 
Get precalculated fractions of proteins 
 
  
    Acidic  
     
   
 
  
    pI < 6.8  
     
   
 
  
    6.8-7.4  
     
   
 
  
    pI > 7.4  
     
   
 
  
    Basic  
     
   
    
 
  
    All  
     
   
 
 
Note: above files contain also dissociation constants (pKa) 
 
Virtual 2D-PAGE plot for 17685 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 
 Summary statistics related to proteome-wise predictions 
 
Protein with the lowest isoelectric point: 
>tr|A0A5E3XFB8|A0A5E3XFB8_9AGAM JmjC domain-containing protein OS=Peniophora sp. CBMAI 1063 OX=718367 GN=PNH_LOCUS12441 PE=4 SV=1 
MM1 pKa = 6.32  MAGWASAWNVTVLPLGDD18 pKa = 4.21  SITYY22 pKa = 9.39  GWEE25 pKa = 3.88  SSDD28 pKa = 3.19  GNGYY32 pKa = 9.98  RR33 pKa = 11.84  EE34 pKa = 4.17  GLLEE38 pKa = 5.25  LIEE41 pKa = 4.47  ASGNAVDD48 pKa = 4.86  YY49 pKa = 10.76  IGSIQAGSMADD60 pKa = 3.26  NWNDD64 pKa = 3.6  GYY66 pKa = 10.55  IGCTIEE72 pKa = 4.67  QIATNGVSALQMVPKK87 pKa = 10.5  VVLLLAGTNDD97 pKa = 2.78  INYY100 pKa = 9.78  QEE102 pKa = 5.11  DD103 pKa = 4.01  LANAPARR110 pKa = 11.84  LDD112 pKa = 3.6  SLIDD116 pKa = 4.06  EE117 pKa = 4.79  IFSYY121 pKa = 10.37  TPQATVLVSDD131 pKa = 4.49  IPLIVDD137 pKa = 3.56  DD138 pKa = 4.37  TLEE141 pKa = 4.44  PLVKK145 pKa = 9.71  TYY147 pKa = 11.02  NAGVLDD153 pKa = 4.2  LVNGRR158 pKa = 11.84  IADD161 pKa = 3.94  GQKK164 pKa = 10.22  LVHH167 pKa = 6.24  VSLDD171 pKa = 3.36  ALTSSEE177 pKa = 4.4  MADD180 pKa = 3.22  EE181 pKa = 4.22  LHH183 pKa = 7.03  PNDD186 pKa = 4.3  EE187 pKa = 4.86  GYY189 pKa = 10.52  QVLAKK194 pKa = 10.18  AWYY197 pKa = 8.78  TGVQTAAAKK206 pKa = 10.16  GWIASS211 pKa = 3.68   
 Molecular weight: 22.53 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.706 
IPC2_protein 3.821 
IPC_protein 3.808 
Toseland    3.592 
ProMoST     3.973 
Dawson      3.795 
Bjellqvist  3.961 
Wikipedia   3.745 
Rodwell     3.63 
Grimsley    3.503 
Solomon     3.795 
Lehninger   3.745 
Nozaki      3.923 
DTASelect   4.151 
Thurlkill   3.656 
EMBOSS      3.745 
Sillero     3.935 
Patrickios  1.036 
IPC_peptide 3.783 
IPC2_peptide  3.91 
IPC2.peptide.svr19  3.828 
 Protein with the highest isoelectric point: 
>tr|A0A5E3WZ03|A0A5E3WZ03_9AGAM Uncharacterized protein OS=Peniophora sp. CBMAI 1063 OX=718367 GN=PNH_LOCUS6806 PE=4 SV=1 
MM1 pKa = 7.39  ATPAMSPTATQPSQGRR17 pKa = 11.84  PSTSPRR23 pKa = 11.84  LGKK26 pKa = 10.4  SGLISSSKK34 pKa = 9.83  RR35 pKa = 11.84  AAPSTMARR43 pKa = 11.84  SRR45 pKa = 11.84  TTPSTKK51 pKa = 10.16  SDD53 pKa = 3.17  TKK55 pKa = 7.97  TTRR58 pKa = 11.84  LPKK61 pKa = 10.22  PSANTAKK68 pKa = 10.01  TSPRR72 pKa = 11.84  VKK74 pKa = 9.84  TSSVPSKK81 pKa = 10.69  VQAATAASKK90 pKa = 11.04  AKK92 pKa = 9.21  TAAPLVKK99 pKa = 9.79  TKK101 pKa = 10.47  AAPISSASKK110 pKa = 9.3  VPAAPRR116 pKa = 11.84  HH117 pKa = 5.43  SSIQASPKK125 pKa = 9.66  LAAKK129 pKa = 10.31  ARR131 pKa = 11.84  TASVASPKK139 pKa = 10.46  AKK141 pKa = 9.29  PAPRR145 pKa = 11.84  ISKK148 pKa = 10.34  LPSSPNKK155 pKa = 9.58  AQAPARR161 pKa = 11.84  AVSAATRR168 pKa = 11.84  SAPVTRR174 pKa = 11.84  SVGKK178 pKa = 8.59  STGSSGAKK186 pKa = 8.86  PAPSVVKK193 pKa = 10.37  ARR195 pKa = 11.84  PASTFSATSKK205 pKa = 7.38  TTVRR209 pKa = 11.84  RR210 pKa = 11.84  ASVLSRR216 pKa = 11.84  ARR218 pKa = 11.84  TEE220 pKa = 3.83  PATAASGIIASPPATPTAPTKK241 pKa = 9.07  GTRR244 pKa = 11.84  SGRR247 pKa = 11.84  SVGWAA252 pKa = 3.08   
 Molecular weight: 25.17 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.466 
IPC2_protein 11.038 
IPC_protein 12.588 
Toseland    12.778 
ProMoST     13.261 
Dawson      12.778 
Bjellqvist  12.764 
Wikipedia   13.247 
Rodwell     12.632 
Grimsley    12.808 
Solomon     13.261 
Lehninger   13.159 
Nozaki      12.764 
DTASelect   12.764 
Thurlkill   12.764 
EMBOSS      13.261 
Sillero     12.764 
Patrickios  12.34 
IPC_peptide 13.261 
IPC2_peptide  12.252 
IPC2.peptide.svr19  9.056 
  
 
Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try ESI  
     
   
 
  
    ChTry ESI  
     
   
 
  
    ArgC ESI  
     
   
 
  
    LysN ESI  
     
   
 
  
    TryLysC ESI  
     
   
 
  
    Try MALDI  
     
   
 
  
    ChTry MALDI  
     
   
 
  
    ArgC MALDI  
     
   
 
  
    LysN MALDI  
     
   
 
  
    TryLysC MALDI  
     
   
 
  
    Try LTQ  
     
   
 
  
    ChTry LTQ  
     
   
 
  
    ArgC LTQ  
     
   
 
  
    LysN LTQ  
     
   
 
  
    TryLysC LTQ  
     
   
 
  
    Try MSlow  
     
   
 
  
    ChTry MSlow  
     
   
 
  
    ArgC MSlow  
     
   
 
  
    LysN MSlow  
     
   
 
  
    TryLysC MSlow  
     
   
 
  
    Try MShigh  
     
   
 
  
    ChTry MShigh  
     
   
 
  
    ArgC MShigh  
     
   
 
  
    LysN MShigh  
     
   
 
  
    TryLysC MShigh  
     
   
   
  
                       
General Statistics 
    
      
        Number of major isoforms
 
        Number of additional isoforms
 
        Number of all proteins
         
        Number of amino acids
 
        Min. Seq. Length
 
        Max. Seq. Length
 
        Avg. Seq. Length
 
        Avg. Mol. Weight
 
       
     
    
      
        17197 
 
        
        488 
 
        
        17685 
         
        8329052
 
        16
 
        5014
 
        471.0
 
        51.77
 
                
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
 
        Cys
 
        Asp
 
        Glu
 
        Phe
 
        Gly
 
        His
 
        Ile
 
        Lys
 
        Leu
 
       
     
    
                 
        9.984 ± 0.021
1.212 ± 0.007
 
        5.834 ± 0.011
5.877 ± 0.016
 
        3.481 ± 0.012
6.6 ± 0.017
 
        2.6 ± 0.008
4.342 ± 0.013
       
        3.8 ± 0.016
9.431 ± 0.024
         
                
     
  
  
  
      
          
        Met
         
        Asn
 
        Gln
 
        Pro
         
        Arg
 
        Ser
 
        Thr
 
        Val
 
        Trp
 
        Tyr
  
       
     
    
                 
        2.034 ± 0.007
3.023 ± 0.009
 
        6.7 ± 0.025
3.48 ± 0.01
 
        6.795 ± 0.016
8.259 ± 0.022
 
        5.945 ± 0.012
6.449 ± 0.013
       
        1.488 ± 0.007
2.608 ± 0.009
         
                
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level 
Most of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here