Peniophora sp. CBMAI 1063
Average proteome isoelectric point is 6.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 17685 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5E3XFB8|A0A5E3XFB8_9AGAM JmjC domain-containing protein OS=Peniophora sp. CBMAI 1063 OX=718367 GN=PNH_LOCUS12441 PE=4 SV=1
MM1 pKa = 6.32 MAGWASAWNVTVLPLGDD18 pKa = 4.21 SITYY22 pKa = 9.39 GWEE25 pKa = 3.88 SSDD28 pKa = 3.19 GNGYY32 pKa = 9.98 RR33 pKa = 11.84 EE34 pKa = 4.17 GLLEE38 pKa = 5.25 LIEE41 pKa = 4.47 ASGNAVDD48 pKa = 4.86 YY49 pKa = 10.76 IGSIQAGSMADD60 pKa = 3.26 NWNDD64 pKa = 3.6 GYY66 pKa = 10.55 IGCTIEE72 pKa = 4.67 QIATNGVSALQMVPKK87 pKa = 10.5 VVLLLAGTNDD97 pKa = 2.78 INYY100 pKa = 9.78 QEE102 pKa = 5.11 DD103 pKa = 4.01 LANAPARR110 pKa = 11.84 LDD112 pKa = 3.6 SLIDD116 pKa = 4.06 EE117 pKa = 4.79 IFSYY121 pKa = 10.37 TPQATVLVSDD131 pKa = 4.49 IPLIVDD137 pKa = 3.56 DD138 pKa = 4.37 TLEE141 pKa = 4.44 PLVKK145 pKa = 9.71 TYY147 pKa = 11.02 NAGVLDD153 pKa = 4.2 LVNGRR158 pKa = 11.84 IADD161 pKa = 3.94 GQKK164 pKa = 10.22 LVHH167 pKa = 6.24 VSLDD171 pKa = 3.36 ALTSSEE177 pKa = 4.4 MADD180 pKa = 3.22 EE181 pKa = 4.22 LHH183 pKa = 7.03 PNDD186 pKa = 4.3 EE187 pKa = 4.86 GYY189 pKa = 10.52 QVLAKK194 pKa = 10.18 AWYY197 pKa = 8.78 TGVQTAAAKK206 pKa = 10.16 GWIASS211 pKa = 3.68
Molecular weight: 22.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.706
IPC2_protein 3.821
IPC_protein 3.808
Toseland 3.592
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.961
Wikipedia 3.745
Rodwell 3.63
Grimsley 3.503
Solomon 3.795
Lehninger 3.745
Nozaki 3.923
DTASelect 4.151
Thurlkill 3.656
EMBOSS 3.745
Sillero 3.935
Patrickios 1.036
IPC_peptide 3.783
IPC2_peptide 3.91
IPC2.peptide.svr19 3.828
Protein with the highest isoelectric point:
>tr|A0A5E3WZ03|A0A5E3WZ03_9AGAM Uncharacterized protein OS=Peniophora sp. CBMAI 1063 OX=718367 GN=PNH_LOCUS6806 PE=4 SV=1
MM1 pKa = 7.39 ATPAMSPTATQPSQGRR17 pKa = 11.84 PSTSPRR23 pKa = 11.84 LGKK26 pKa = 10.4 SGLISSSKK34 pKa = 9.83 RR35 pKa = 11.84 AAPSTMARR43 pKa = 11.84 SRR45 pKa = 11.84 TTPSTKK51 pKa = 10.16 SDD53 pKa = 3.17 TKK55 pKa = 7.97 TTRR58 pKa = 11.84 LPKK61 pKa = 10.22 PSANTAKK68 pKa = 10.01 TSPRR72 pKa = 11.84 VKK74 pKa = 9.84 TSSVPSKK81 pKa = 10.69 VQAATAASKK90 pKa = 11.04 AKK92 pKa = 9.21 TAAPLVKK99 pKa = 9.79 TKK101 pKa = 10.47 AAPISSASKK110 pKa = 9.3 VPAAPRR116 pKa = 11.84 HH117 pKa = 5.43 SSIQASPKK125 pKa = 9.66 LAAKK129 pKa = 10.31 ARR131 pKa = 11.84 TASVASPKK139 pKa = 10.46 AKK141 pKa = 9.29 PAPRR145 pKa = 11.84 ISKK148 pKa = 10.34 LPSSPNKK155 pKa = 9.58 AQAPARR161 pKa = 11.84 AVSAATRR168 pKa = 11.84 SAPVTRR174 pKa = 11.84 SVGKK178 pKa = 8.59 STGSSGAKK186 pKa = 8.86 PAPSVVKK193 pKa = 10.37 ARR195 pKa = 11.84 PASTFSATSKK205 pKa = 7.38 TTVRR209 pKa = 11.84 RR210 pKa = 11.84 ASVLSRR216 pKa = 11.84 ARR218 pKa = 11.84 TEE220 pKa = 3.83 PATAASGIIASPPATPTAPTKK241 pKa = 9.07 GTRR244 pKa = 11.84 SGRR247 pKa = 11.84 SVGWAA252 pKa = 3.08
Molecular weight: 25.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.466
IPC2_protein 11.038
IPC_protein 12.588
Toseland 12.778
ProMoST 13.261
Dawson 12.778
Bjellqvist 12.764
Wikipedia 13.247
Rodwell 12.632
Grimsley 12.808
Solomon 13.261
Lehninger 13.159
Nozaki 12.764
DTASelect 12.764
Thurlkill 12.764
EMBOSS 13.261
Sillero 12.764
Patrickios 12.34
IPC_peptide 13.261
IPC2_peptide 12.252
IPC2.peptide.svr19 9.056
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
17197
488
17685
8329052
16
5014
471.0
51.77
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.984 ± 0.021
1.212 ± 0.007
5.834 ± 0.011
5.877 ± 0.016
3.481 ± 0.012
6.6 ± 0.017
2.6 ± 0.008
4.342 ± 0.013
3.8 ± 0.016
9.431 ± 0.024
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.034 ± 0.007
3.023 ± 0.009
6.7 ± 0.025
3.48 ± 0.01
6.795 ± 0.016
8.259 ± 0.022
5.945 ± 0.012
6.449 ± 0.013
1.488 ± 0.007
2.608 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here