Amino acid dipepetide frequency for Mycobacterium phage Blexus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.186AlaAla: 15.186 ± 2.052
1.023AlaCys: 1.023 ± 0.262
7.108AlaAsp: 7.108 ± 0.584
7.485AlaGlu: 7.485 ± 0.855
3.069AlaPhe: 3.069 ± 0.458
9.855AlaGly: 9.855 ± 1.256
2.746AlaHis: 2.746 ± 0.416
4.254AlaIle: 4.254 ± 0.501
4.362AlaLys: 4.362 ± 0.404
7.701AlaLeu: 7.701 ± 0.623
2.639AlaMet: 2.639 ± 0.401
2.693AlaAsn: 2.693 ± 0.412
4.9AlaPro: 4.9 ± 0.521
3.716AlaGln: 3.716 ± 0.462
7.431AlaArg: 7.431 ± 0.835
5.493AlaSer: 5.493 ± 0.603
6.3AlaThr: 6.3 ± 0.524
7.001AlaVal: 7.001 ± 0.623
2.854AlaTrp: 2.854 ± 0.656
2.046AlaTyr: 2.046 ± 0.318
0.0AlaXaa: 0.0 ± 0.0
Cys
0.915CysAla: 0.915 ± 0.236
0.108CysCys: 0.108 ± 0.074
1.185CysAsp: 1.185 ± 0.325
0.7CysGlu: 0.7 ± 0.169
0.162CysPhe: 0.162 ± 0.086
1.562CysGly: 1.562 ± 0.352
0.269CysHis: 0.269 ± 0.121
0.215CysIle: 0.215 ± 0.114
0.485CysLys: 0.485 ± 0.183
0.646CysLeu: 0.646 ± 0.199
0.162CysMet: 0.162 ± 0.098
0.431CysAsn: 0.431 ± 0.124
1.185CysPro: 1.185 ± 0.321
0.162CysGln: 0.162 ± 0.106
0.646CysArg: 0.646 ± 0.197
0.808CysSer: 0.808 ± 0.267
0.7CysThr: 0.7 ± 0.186
0.592CysVal: 0.592 ± 0.171
0.377CysTrp: 0.377 ± 0.144
0.162CysTyr: 0.162 ± 0.093
0.0CysXaa: 0.0 ± 0.0
Asp
7.485AspAla: 7.485 ± 0.625
0.915AspCys: 0.915 ± 0.269
4.2AspAsp: 4.2 ± 0.5
3.393AspGlu: 3.393 ± 0.407
1.454AspPhe: 1.454 ± 0.224
6.839AspGly: 6.839 ± 0.628
1.239AspHis: 1.239 ± 0.253
2.369AspIle: 2.369 ± 0.386
1.723AspLys: 1.723 ± 0.284
5.547AspLeu: 5.547 ± 0.466
1.185AspMet: 1.185 ± 0.283
1.292AspAsn: 1.292 ± 0.258
4.9AspPro: 4.9 ± 0.557
2.423AspGln: 2.423 ± 0.344
4.793AspArg: 4.793 ± 0.648
3.608AspSer: 3.608 ± 0.523
3.985AspThr: 3.985 ± 0.469
3.823AspVal: 3.823 ± 0.37
1.777AspTrp: 1.777 ± 0.322
2.1AspTyr: 2.1 ± 0.269
0.0AspXaa: 0.0 ± 0.0
Glu
6.354GluAla: 6.354 ± 0.753
0.862GluCys: 0.862 ± 0.228
2.746GluAsp: 2.746 ± 0.369
2.423GluGlu: 2.423 ± 0.491
2.154GluPhe: 2.154 ± 0.321
3.069GluGly: 3.069 ± 0.401
1.831GluHis: 1.831 ± 0.354
2.154GluIle: 2.154 ± 0.315
1.777GluLys: 1.777 ± 0.342
6.139GluLeu: 6.139 ± 0.699
1.454GluMet: 1.454 ± 0.284
2.1GluAsn: 2.1 ± 0.273
2.746GluPro: 2.746 ± 0.466
2.746GluGln: 2.746 ± 0.345
4.954GluArg: 4.954 ± 0.66
3.877GluSer: 3.877 ± 0.622
3.877GluThr: 3.877 ± 0.601
4.254GluVal: 4.254 ± 0.517
1.831GluTrp: 1.831 ± 0.282
1.831GluTyr: 1.831 ± 0.316
0.0GluXaa: 0.0 ± 0.0
Phe
3.393PheAla: 3.393 ± 0.346
0.162PheCys: 0.162 ± 0.101
2.477PheAsp: 2.477 ± 0.486
1.777PheGlu: 1.777 ± 0.392
0.915PhePhe: 0.915 ± 0.244
3.069PheGly: 3.069 ± 0.781
0.431PheHis: 0.431 ± 0.146
1.239PheIle: 1.239 ± 0.348
1.023PheLys: 1.023 ± 0.247
1.831PheLeu: 1.831 ± 0.331
0.7PheMet: 0.7 ± 0.188
1.239PheAsn: 1.239 ± 0.302
1.562PhePro: 1.562 ± 0.331
1.346PheGln: 1.346 ± 0.287
1.777PheArg: 1.777 ± 0.285
1.508PheSer: 1.508 ± 0.264
2.262PheThr: 2.262 ± 0.374
2.262PheVal: 2.262 ± 0.34
0.592PheTrp: 0.592 ± 0.157
1.077PheTyr: 1.077 ± 0.243
0.0PheXaa: 0.0 ± 0.0
Gly
8.239GlyAla: 8.239 ± 1.138
0.915GlyCys: 0.915 ± 0.258
6.247GlyAsp: 6.247 ± 0.601
4.416GlyGlu: 4.416 ± 0.519
3.069GlyPhe: 3.069 ± 0.432
10.285GlyGly: 10.285 ± 2.67
1.939GlyHis: 1.939 ± 0.299
3.77GlyIle: 3.77 ± 0.532
2.693GlyLys: 2.693 ± 0.363
6.085GlyLeu: 6.085 ± 0.601
2.154GlyMet: 2.154 ± 0.43
3.285GlyAsn: 3.285 ± 0.453
4.308GlyPro: 4.308 ± 0.487
2.8GlyGln: 2.8 ± 0.619
5.493GlyArg: 5.493 ± 0.529
5.439GlySer: 5.439 ± 0.858
6.085GlyThr: 6.085 ± 0.843
6.031GlyVal: 6.031 ± 0.695
2.531GlyTrp: 2.531 ± 0.363
1.939GlyTyr: 1.939 ± 0.31
0.0GlyXaa: 0.0 ± 0.0
His
2.046HisAla: 2.046 ± 0.359
0.323HisCys: 0.323 ± 0.153
1.239HisAsp: 1.239 ± 0.23
1.023HisGlu: 1.023 ± 0.253
0.431HisPhe: 0.431 ± 0.139
1.777HisGly: 1.777 ± 0.293
0.808HisHis: 0.808 ± 0.256
1.454HisIle: 1.454 ± 0.262
0.862HisLys: 0.862 ± 0.221
1.939HisLeu: 1.939 ± 0.324
0.323HisMet: 0.323 ± 0.109
0.862HisAsn: 0.862 ± 0.206
1.346HisPro: 1.346 ± 0.267
0.808HisGln: 0.808 ± 0.222
1.831HisArg: 1.831 ± 0.351
0.915HisSer: 0.915 ± 0.185
1.292HisThr: 1.292 ± 0.306
1.669HisVal: 1.669 ± 0.293
0.431HisTrp: 0.431 ± 0.136
0.808HisTyr: 0.808 ± 0.193
0.0HisXaa: 0.0 ± 0.0
Ile
5.116IleAla: 5.116 ± 0.554
0.592IleCys: 0.592 ± 0.213
3.5IleAsp: 3.5 ± 0.418
3.446IleGlu: 3.446 ± 0.363
0.808IlePhe: 0.808 ± 0.23
3.77IleGly: 3.77 ± 0.367
1.723IleHis: 1.723 ± 0.305
1.454IleIle: 1.454 ± 0.224
1.292IleLys: 1.292 ± 0.263
2.693IleLeu: 2.693 ± 0.454
0.323IleMet: 0.323 ± 0.128
1.885IleAsn: 1.885 ± 0.329
3.231IlePro: 3.231 ± 0.37
1.508IleGln: 1.508 ± 0.247
2.531IleArg: 2.531 ± 0.443
2.262IleSer: 2.262 ± 0.393
3.285IleThr: 3.285 ± 0.432
3.231IleVal: 3.231 ± 0.363
0.915IleTrp: 0.915 ± 0.211
0.646IleTyr: 0.646 ± 0.139
0.0IleXaa: 0.0 ± 0.0
Lys
3.5LysAla: 3.5 ± 0.459
0.485LysCys: 0.485 ± 0.151
1.939LysAsp: 1.939 ± 0.303
1.723LysGlu: 1.723 ± 0.275
1.292LysPhe: 1.292 ± 0.216
2.369LysGly: 2.369 ± 0.348
0.969LysHis: 0.969 ± 0.251
0.808LysIle: 0.808 ± 0.2
1.239LysLys: 1.239 ± 0.405
2.208LysLeu: 2.208 ± 0.431
0.485LysMet: 0.485 ± 0.143
1.023LysAsn: 1.023 ± 0.294
2.639LysPro: 2.639 ± 0.545
1.723LysGln: 1.723 ± 0.243
2.369LysArg: 2.369 ± 0.365
2.046LysSer: 2.046 ± 0.364
2.369LysThr: 2.369 ± 0.395
2.423LysVal: 2.423 ± 0.431
0.7LysTrp: 0.7 ± 0.211
0.592LysTyr: 0.592 ± 0.201
0.0LysXaa: 0.0 ± 0.0
Leu
7.485LeuAla: 7.485 ± 0.763
0.431LeuCys: 0.431 ± 0.17
4.47LeuAsp: 4.47 ± 0.473
4.362LeuGlu: 4.362 ± 0.524
2.639LeuPhe: 2.639 ± 0.316
5.17LeuGly: 5.17 ± 0.516
1.023LeuHis: 1.023 ± 0.226
3.608LeuIle: 3.608 ± 0.433
2.1LeuLys: 2.1 ± 0.336
4.847LeuLeu: 4.847 ± 0.574
1.508LeuMet: 1.508 ± 0.272
2.8LeuAsn: 2.8 ± 0.428
5.277LeuPro: 5.277 ± 0.647
3.123LeuGln: 3.123 ± 0.472
4.847LeuArg: 4.847 ± 0.647
5.87LeuSer: 5.87 ± 0.555
5.439LeuThr: 5.439 ± 0.526
5.331LeuVal: 5.331 ± 0.526
1.4LeuTrp: 1.4 ± 0.307
2.316LeuTyr: 2.316 ± 0.371
0.0LeuXaa: 0.0 ± 0.0
Met
2.262MetAla: 2.262 ± 0.396
0.162MetCys: 0.162 ± 0.1
1.131MetAsp: 1.131 ± 0.273
1.077MetGlu: 1.077 ± 0.214
0.592MetPhe: 0.592 ± 0.188
1.939MetGly: 1.939 ± 0.267
0.054MetHis: 0.054 ± 0.058
1.023MetIle: 1.023 ± 0.233
0.539MetLys: 0.539 ± 0.179
2.046MetLeu: 2.046 ± 0.321
0.539MetMet: 0.539 ± 0.192
0.808MetAsn: 0.808 ± 0.184
1.077MetPro: 1.077 ± 0.228
0.431MetGln: 0.431 ± 0.142
1.885MetArg: 1.885 ± 0.31
2.854MetSer: 2.854 ± 0.332
1.992MetThr: 1.992 ± 0.305
1.346MetVal: 1.346 ± 0.306
0.323MetTrp: 0.323 ± 0.129
0.377MetTyr: 0.377 ± 0.16
0.0MetXaa: 0.0 ± 0.0
Asn
3.554AsnAla: 3.554 ± 0.365
0.323AsnCys: 0.323 ± 0.152
1.885AsnAsp: 1.885 ± 0.328
1.723AsnGlu: 1.723 ± 0.385
0.915AsnPhe: 0.915 ± 0.245
4.039AsnGly: 4.039 ± 0.525
0.754AsnHis: 0.754 ± 0.186
1.616AsnIle: 1.616 ± 0.42
0.862AsnLys: 0.862 ± 0.235
2.154AsnLeu: 2.154 ± 0.426
0.754AsnMet: 0.754 ± 0.178
1.616AsnAsn: 1.616 ± 0.285
2.746AsnPro: 2.746 ± 0.386
0.969AsnGln: 0.969 ± 0.299
2.208AsnArg: 2.208 ± 0.441
1.723AsnSer: 1.723 ± 0.278
1.939AsnThr: 1.939 ± 0.292
2.208AsnVal: 2.208 ± 0.287
0.754AsnTrp: 0.754 ± 0.221
0.915AsnTyr: 0.915 ± 0.178
0.0AsnXaa: 0.0 ± 0.0
Pro
5.331ProAla: 5.331 ± 0.648
0.646ProCys: 0.646 ± 0.177
4.093ProAsp: 4.093 ± 0.516
4.577ProGlu: 4.577 ± 0.474
2.208ProPhe: 2.208 ± 0.379
7.485ProGly: 7.485 ± 0.693
1.346ProHis: 1.346 ± 0.237
1.723ProIle: 1.723 ± 0.311
2.316ProLys: 2.316 ± 0.423
4.254ProLeu: 4.254 ± 0.523
1.669ProMet: 1.669 ± 0.322
2.369ProAsn: 2.369 ± 0.363
4.631ProPro: 4.631 ± 0.694
2.316ProGln: 2.316 ± 0.408
3.016ProArg: 3.016 ± 0.511
3.5ProSer: 3.5 ± 0.423
3.016ProThr: 3.016 ± 0.395
4.577ProVal: 4.577 ± 0.546
0.969ProTrp: 0.969 ± 0.225
1.4ProTyr: 1.4 ± 0.271
0.0ProXaa: 0.0 ± 0.0
Gln
4.308GlnAla: 4.308 ± 0.637
0.269GlnCys: 0.269 ± 0.148
1.562GlnAsp: 1.562 ± 0.259
1.723GlnGlu: 1.723 ± 0.309
1.023GlnPhe: 1.023 ± 0.224
2.369GlnGly: 2.369 ± 0.393
1.077GlnHis: 1.077 ± 0.282
2.316GlnIle: 2.316 ± 0.344
1.346GlnLys: 1.346 ± 0.218
3.285GlnLeu: 3.285 ± 0.365
0.808GlnMet: 0.808 ± 0.19
0.754GlnAsn: 0.754 ± 0.171
2.531GlnPro: 2.531 ± 0.382
1.077GlnGln: 1.077 ± 0.225
2.531GlnArg: 2.531 ± 0.391
2.154GlnSer: 2.154 ± 0.332
1.885GlnThr: 1.885 ± 0.342
2.369GlnVal: 2.369 ± 0.356
0.862GlnTrp: 0.862 ± 0.232
1.185GlnTyr: 1.185 ± 0.291
0.0GlnXaa: 0.0 ± 0.0
Arg
6.893ArgAla: 6.893 ± 0.7
1.239ArgCys: 1.239 ± 0.357
4.685ArgAsp: 4.685 ± 0.57
5.116ArgGlu: 5.116 ± 0.699
2.154ArgPhe: 2.154 ± 0.456
3.985ArgGly: 3.985 ± 0.427
1.185ArgHis: 1.185 ± 0.243
4.093ArgIle: 4.093 ± 0.549
2.585ArgLys: 2.585 ± 0.455
5.17ArgLeu: 5.17 ± 0.682
2.369ArgMet: 2.369 ± 0.336
2.316ArgAsn: 2.316 ± 0.371
3.77ArgPro: 3.77 ± 0.426
1.831ArgGln: 1.831 ± 0.352
5.547ArgArg: 5.547 ± 0.733
3.662ArgSer: 3.662 ± 0.414
3.339ArgThr: 3.339 ± 0.467
4.685ArgVal: 4.685 ± 0.567
1.885ArgTrp: 1.885 ± 0.355
1.939ArgTyr: 1.939 ± 0.348
0.0ArgXaa: 0.0 ± 0.0
Ser
7.054SerAla: 7.054 ± 1.327
0.485SerCys: 0.485 ± 0.17
4.146SerAsp: 4.146 ± 0.489
3.069SerGlu: 3.069 ± 0.425
1.939SerPhe: 1.939 ± 0.445
5.816SerGly: 5.816 ± 0.635
1.023SerHis: 1.023 ± 0.218
3.177SerIle: 3.177 ± 0.444
2.262SerLys: 2.262 ± 0.298
3.662SerLeu: 3.662 ± 0.419
1.616SerMet: 1.616 ± 0.382
2.262SerAsn: 2.262 ± 0.338
3.77SerPro: 3.77 ± 0.373
1.939SerGln: 1.939 ± 0.261
3.716SerArg: 3.716 ± 0.366
3.662SerSer: 3.662 ± 0.604
3.716SerThr: 3.716 ± 0.446
4.685SerVal: 4.685 ± 0.436
0.969SerTrp: 0.969 ± 0.199
1.508SerTyr: 1.508 ± 0.244
0.0SerXaa: 0.0 ± 0.0
Thr
6.624ThrAla: 6.624 ± 0.647
0.592ThrCys: 0.592 ± 0.179
4.146ThrAsp: 4.146 ± 0.549
3.662ThrGlu: 3.662 ± 0.469
1.831ThrPhe: 1.831 ± 0.315
5.977ThrGly: 5.977 ± 0.632
1.185ThrHis: 1.185 ± 0.27
3.231ThrIle: 3.231 ± 0.408
1.992ThrLys: 1.992 ± 0.41
4.47ThrLeu: 4.47 ± 0.533
1.4ThrMet: 1.4 ± 0.242
2.208ThrAsn: 2.208 ± 0.315
4.362ThrPro: 4.362 ± 0.71
1.723ThrGln: 1.723 ± 0.226
3.931ThrArg: 3.931 ± 0.508
3.823ThrSer: 3.823 ± 0.438
4.47ThrThr: 4.47 ± 0.686
5.654ThrVal: 5.654 ± 0.542
1.185ThrTrp: 1.185 ± 0.278
1.292ThrTyr: 1.292 ± 0.238
0.0ThrXaa: 0.0 ± 0.0
Val
7.377ValAla: 7.377 ± 0.551
1.239ValCys: 1.239 ± 0.261
5.116ValAsp: 5.116 ± 0.621
4.631ValGlu: 4.631 ± 0.641
2.477ValPhe: 2.477 ± 0.373
5.331ValGly: 5.331 ± 0.67
1.669ValHis: 1.669 ± 0.321
3.177ValIle: 3.177 ± 0.408
2.154ValLys: 2.154 ± 0.33
5.924ValLeu: 5.924 ± 0.662
1.185ValMet: 1.185 ± 0.186
2.154ValAsn: 2.154 ± 0.411
4.254ValPro: 4.254 ± 0.401
2.962ValGln: 2.962 ± 0.325
4.577ValArg: 4.577 ± 0.507
4.631ValSer: 4.631 ± 0.542
4.685ValThr: 4.685 ± 0.567
5.654ValVal: 5.654 ± 0.576
1.616ValTrp: 1.616 ± 0.367
0.808ValTyr: 0.808 ± 0.2
0.0ValXaa: 0.0 ± 0.0
Trp
2.477TrpAla: 2.477 ± 0.361
0.269TrpCys: 0.269 ± 0.122
1.508TrpAsp: 1.508 ± 0.271
1.077TrpGlu: 1.077 ± 0.335
0.7TrpPhe: 0.7 ± 0.182
0.915TrpGly: 0.915 ± 0.232
0.485TrpHis: 0.485 ± 0.157
1.346TrpIle: 1.346 ± 0.293
0.754TrpLys: 0.754 ± 0.162
1.777TrpLeu: 1.777 ± 0.319
0.808TrpMet: 0.808 ± 0.252
0.7TrpAsn: 0.7 ± 0.259
0.969TrpPro: 0.969 ± 0.266
1.131TrpGln: 1.131 ± 0.274
2.262TrpArg: 2.262 ± 0.377
1.454TrpSer: 1.454 ± 0.449
1.723TrpThr: 1.723 ± 0.331
1.669TrpVal: 1.669 ± 0.45
1.077TrpTrp: 1.077 ± 0.232
0.485TrpTyr: 0.485 ± 0.166
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.262TyrAla: 2.262 ± 0.35
0.431TyrCys: 0.431 ± 0.139
1.669TyrAsp: 1.669 ± 0.308
1.777TyrGlu: 1.777 ± 0.282
0.808TyrPhe: 0.808 ± 0.194
2.1TyrGly: 2.1 ± 0.403
0.431TyrHis: 0.431 ± 0.178
1.185TyrIle: 1.185 ± 0.224
0.539TyrLys: 0.539 ± 0.18
1.669TyrLeu: 1.669 ± 0.314
0.323TyrMet: 0.323 ± 0.119
0.862TyrAsn: 0.862 ± 0.208
1.292TyrPro: 1.292 ± 0.2
0.7TyrGln: 0.7 ± 0.201
2.1TyrArg: 2.1 ± 0.43
1.185TyrSer: 1.185 ± 0.238
1.4TyrThr: 1.4 ± 0.269
2.154TyrVal: 2.154 ± 0.323
0.539TyrTrp: 0.539 ± 0.159
0.431TyrTyr: 0.431 ± 0.115
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 101 proteins (18571 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski