Marinobacter sp. BSs20148
Average proteome isoelectric point is 6.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3885 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|M1FC69|M1FC69_9ALTE Proline iminopeptidase OS=Marinobacter sp. BSs20148 OX=490759 GN=pip PE=3 SV=1
MM1 pKa = 7.45 SVVQQQVSAPLFFSNSAAAKK21 pKa = 9.59 AKK23 pKa = 10.27 EE24 pKa = 4.29 LIDD27 pKa = 3.73 EE28 pKa = 4.84 EE29 pKa = 4.94 GNADD33 pKa = 3.72 LKK35 pKa = 11.22 LRR37 pKa = 11.84 VYY39 pKa = 9.06 VTGGGCSGFQYY50 pKa = 10.84 GFSFDD55 pKa = 4.68 DD56 pKa = 4.18 AQDD59 pKa = 4.24 DD60 pKa = 3.85 EE61 pKa = 4.54 DD62 pKa = 4.74 TIIEE66 pKa = 4.06 RR67 pKa = 11.84 DD68 pKa = 3.83 GVKK71 pKa = 10.65 LLVDD75 pKa = 3.8 PMSYY79 pKa = 10.37 QYY81 pKa = 11.59 LVGSTVDD88 pKa = 3.55 YY89 pKa = 11.59 LEE91 pKa = 4.76 GLQGSQFVVNNPNASSTCGCGSSFSII117 pKa = 5.02
Molecular weight: 12.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.742
IPC2_protein 3.897
IPC_protein 3.846
Toseland 3.63
ProMoST 4.012
Dawson 3.834
Bjellqvist 3.999
Wikipedia 3.783
Rodwell 3.668
Grimsley 3.541
Solomon 3.821
Lehninger 3.783
Nozaki 3.961
DTASelect 4.19
Thurlkill 3.694
EMBOSS 3.795
Sillero 3.961
Patrickios 0.846
IPC_peptide 3.821
IPC2_peptide 3.935
IPC2.peptide.svr19 3.861
Protein with the highest isoelectric point:
>tr|M1FMA5|M1FMA5_9ALTE Uncharacterized protein OS=Marinobacter sp. BSs20148 OX=490759 GN=MRBBS_3512 PE=4 SV=1
MM1 pKa = 7.66 RR2 pKa = 11.84 PVPISIAVVVMLVSLSGAAKK22 pKa = 9.74 ADD24 pKa = 3.68 YY25 pKa = 10.48 YY26 pKa = 11.17 RR27 pKa = 11.84 WTDD30 pKa = 3.28 ANGVTHH36 pKa = 6.82 FTDD39 pKa = 3.41 KK40 pKa = 10.88 PKK42 pKa = 10.62 TPGSQAINFHH52 pKa = 6.07 QPTVIPIADD61 pKa = 3.51 NNRR64 pKa = 11.84 RR65 pKa = 11.84 RR66 pKa = 11.84 GDD68 pKa = 3.56 RR69 pKa = 11.84 LQGLQPDD76 pKa = 4.16 TPKK79 pKa = 11.08 NSVQGKK85 pKa = 6.93 AQKK88 pKa = 9.48 PAQANAAEE96 pKa = 4.41 IAQQKK101 pKa = 7.36 TCEE104 pKa = 4.05 NYY106 pKa = 10.48 ADD108 pKa = 4.71 RR109 pKa = 11.84 IGNIEE114 pKa = 3.76 SRR116 pKa = 11.84 LRR118 pKa = 11.84 AGGYY122 pKa = 8.5 SASRR126 pKa = 11.84 GNRR129 pKa = 11.84 LRR131 pKa = 11.84 RR132 pKa = 11.84 DD133 pKa = 3.42 RR134 pKa = 11.84 RR135 pKa = 11.84 EE136 pKa = 3.94 LASKK140 pKa = 8.75 RR141 pKa = 11.84 TWEE144 pKa = 4.07 CLRR147 pKa = 11.84 RR148 pKa = 3.76
Molecular weight: 16.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.346
IPC2_protein 9.692
IPC_protein 10.57
Toseland 10.745
ProMoST 10.687
Dawson 10.833
Bjellqvist 10.57
Wikipedia 11.067
Rodwell 11.008
Grimsley 10.877
Solomon 10.979
Lehninger 10.935
Nozaki 10.73
DTASelect 10.555
Thurlkill 10.745
EMBOSS 11.155
Sillero 10.774
Patrickios 10.745
IPC_peptide 10.979
IPC2_peptide 9.589
IPC2.peptide.svr19 8.69
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3885
0
3885
1180275
37
5235
303.8
33.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.21 ± 0.046
0.99 ± 0.014
5.497 ± 0.036
5.861 ± 0.041
3.851 ± 0.03
7.788 ± 0.041
2.208 ± 0.02
5.526 ± 0.031
3.807 ± 0.037
10.818 ± 0.06
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.697 ± 0.018
3.516 ± 0.028
4.55 ± 0.026
4.266 ± 0.032
6.005 ± 0.033
6.068 ± 0.029
5.185 ± 0.028
7.298 ± 0.031
1.339 ± 0.017
2.521 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here