Amino acid dipepetide frequency for Gordonia phage Phinally

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.105AlaAla: 19.105 ± 2.163
0.995AlaCys: 0.995 ± 0.237
9.265AlaAsp: 9.265 ± 0.784
8.061AlaGlu: 8.061 ± 0.842
2.774AlaPhe: 2.774 ± 0.517
9.265AlaGly: 9.265 ± 0.892
2.041AlaHis: 2.041 ± 0.371
5.182AlaIle: 5.182 ± 0.6
2.879AlaLys: 2.879 ± 0.441
10.521AlaLeu: 10.521 ± 0.777
3.141AlaMet: 3.141 ± 0.454
2.826AlaAsn: 2.826 ± 0.424
7.276AlaPro: 7.276 ± 0.51
5.182AlaGln: 5.182 ± 0.598
9.997AlaArg: 9.997 ± 0.717
5.339AlaSer: 5.339 ± 0.606
7.433AlaThr: 7.433 ± 0.829
8.218AlaVal: 8.218 ± 0.74
2.512AlaTrp: 2.512 ± 0.318
2.565AlaTyr: 2.565 ± 0.319
0.0AlaXaa: 0.0 ± 0.0
Cys
0.785CysAla: 0.785 ± 0.199
0.157CysCys: 0.157 ± 0.111
0.837CysAsp: 0.837 ± 0.248
0.628CysGlu: 0.628 ± 0.243
0.209CysPhe: 0.209 ± 0.096
1.256CysGly: 1.256 ± 0.332
0.209CysHis: 0.209 ± 0.112
0.471CysIle: 0.471 ± 0.17
0.105CysLys: 0.105 ± 0.066
0.262CysLeu: 0.262 ± 0.142
0.157CysMet: 0.157 ± 0.085
0.471CysAsn: 0.471 ± 0.143
1.152CysPro: 1.152 ± 0.275
0.262CysGln: 0.262 ± 0.118
0.733CysArg: 0.733 ± 0.242
0.471CysSer: 0.471 ± 0.136
0.576CysThr: 0.576 ± 0.197
0.314CysVal: 0.314 ± 0.132
0.105CysTrp: 0.105 ± 0.085
0.052CysTyr: 0.052 ± 0.05
0.0CysXaa: 0.0 ± 0.0
Asp
9.526AspAla: 9.526 ± 0.839
0.837AspCys: 0.837 ± 0.235
7.642AspAsp: 7.642 ± 0.799
6.229AspGlu: 6.229 ± 0.906
0.995AspPhe: 0.995 ± 0.22
6.805AspGly: 6.805 ± 0.606
1.518AspHis: 1.518 ± 0.382
2.094AspIle: 2.094 ± 0.231
1.466AspLys: 1.466 ± 0.238
5.234AspLeu: 5.234 ± 0.66
1.57AspMet: 1.57 ± 0.253
1.832AspAsn: 1.832 ± 0.301
6.019AspPro: 6.019 ± 0.652
2.251AspGln: 2.251 ± 0.39
4.763AspArg: 4.763 ± 0.411
2.879AspSer: 2.879 ± 0.483
4.92AspThr: 4.92 ± 0.496
5.182AspVal: 5.182 ± 0.459
1.78AspTrp: 1.78 ± 0.245
1.309AspTyr: 1.309 ± 0.24
0.0AspXaa: 0.0 ± 0.0
Glu
6.281GluAla: 6.281 ± 0.491
0.471GluCys: 0.471 ± 0.174
2.565GluAsp: 2.565 ± 0.391
1.361GluGlu: 1.361 ± 0.277
2.094GluPhe: 2.094 ± 0.23
3.245GluGly: 3.245 ± 0.386
1.989GluHis: 1.989 ± 0.381
2.041GluIle: 2.041 ± 0.297
0.68GluLys: 0.68 ± 0.206
5.496GluLeu: 5.496 ± 0.665
1.361GluMet: 1.361 ± 0.253
1.675GluAsn: 1.675 ± 0.356
3.455GluPro: 3.455 ± 0.489
4.187GluGln: 4.187 ± 0.602
4.449GluArg: 4.449 ± 0.672
2.46GluSer: 2.46 ± 0.425
3.245GluThr: 3.245 ± 0.45
4.397GluVal: 4.397 ± 0.573
1.937GluTrp: 1.937 ± 0.321
1.727GluTyr: 1.727 ± 0.31
0.0GluXaa: 0.0 ± 0.0
Phe
2.826PheAla: 2.826 ± 0.467
0.262PheCys: 0.262 ± 0.152
2.355PheAsp: 2.355 ± 0.37
1.361PheGlu: 1.361 ± 0.284
0.628PhePhe: 0.628 ± 0.291
2.251PheGly: 2.251 ± 0.362
0.733PheHis: 0.733 ± 0.235
0.733PheIle: 0.733 ± 0.251
0.68PheLys: 0.68 ± 0.23
1.413PheLeu: 1.413 ± 0.262
0.471PheMet: 0.471 ± 0.168
0.733PheAsn: 0.733 ± 0.218
1.256PhePro: 1.256 ± 0.31
0.628PheGln: 0.628 ± 0.198
1.309PheArg: 1.309 ± 0.282
0.785PheSer: 0.785 ± 0.168
1.832PheThr: 1.832 ± 0.256
1.937PheVal: 1.937 ± 0.281
0.523PheTrp: 0.523 ± 0.165
0.314PheTyr: 0.314 ± 0.135
0.0PheXaa: 0.0 ± 0.0
Gly
9.369GlyAla: 9.369 ± 1.062
0.471GlyCys: 0.471 ± 0.159
6.281GlyAsp: 6.281 ± 0.623
3.978GlyGlu: 3.978 ± 0.412
1.675GlyPhe: 1.675 ± 0.359
8.636GlyGly: 8.636 ± 1.396
1.989GlyHis: 1.989 ± 0.343
4.449GlyIle: 4.449 ± 0.568
2.46GlyLys: 2.46 ± 0.45
4.92GlyLeu: 4.92 ± 0.599
1.884GlyMet: 1.884 ± 0.359
2.931GlyAsn: 2.931 ± 0.469
3.664GlyPro: 3.664 ± 0.514
3.088GlyGln: 3.088 ± 0.412
5.391GlyArg: 5.391 ± 0.603
5.025GlySer: 5.025 ± 0.769
5.967GlyThr: 5.967 ± 0.843
6.647GlyVal: 6.647 ± 0.587
1.413GlyTrp: 1.413 ± 0.279
2.251GlyTyr: 2.251 ± 0.396
0.0GlyXaa: 0.0 ± 0.0
His
2.198HisAla: 2.198 ± 0.401
0.262HisCys: 0.262 ± 0.114
1.727HisAsp: 1.727 ± 0.368
1.361HisGlu: 1.361 ± 0.261
0.314HisPhe: 0.314 ± 0.13
1.675HisGly: 1.675 ± 0.313
0.576HisHis: 0.576 ± 0.198
0.995HisIle: 0.995 ± 0.279
0.209HisLys: 0.209 ± 0.108
2.041HisLeu: 2.041 ± 0.437
0.576HisMet: 0.576 ± 0.171
0.471HisAsn: 0.471 ± 0.17
1.466HisPro: 1.466 ± 0.303
0.89HisGln: 0.89 ± 0.185
2.408HisArg: 2.408 ± 0.411
0.785HisSer: 0.785 ± 0.219
1.623HisThr: 1.623 ± 0.396
1.57HisVal: 1.57 ± 0.277
0.262HisTrp: 0.262 ± 0.12
0.576HisTyr: 0.576 ± 0.221
0.0HisXaa: 0.0 ± 0.0
Ile
5.915IleAla: 5.915 ± 0.705
0.314IleCys: 0.314 ± 0.131
4.135IleAsp: 4.135 ± 0.502
2.984IleGlu: 2.984 ± 0.373
0.523IlePhe: 0.523 ± 0.163
4.24IleGly: 4.24 ± 0.798
0.628IleHis: 0.628 ± 0.202
1.466IleIle: 1.466 ± 0.256
1.466IleLys: 1.466 ± 0.433
2.251IleLeu: 2.251 ± 0.335
0.471IleMet: 0.471 ± 0.157
1.78IleAsn: 1.78 ± 0.28
2.565IlePro: 2.565 ± 0.386
0.995IleGln: 0.995 ± 0.255
2.617IleArg: 2.617 ± 0.34
1.518IleSer: 1.518 ± 0.339
3.402IleThr: 3.402 ± 0.386
3.559IleVal: 3.559 ± 0.404
0.733IleTrp: 0.733 ± 0.191
0.733IleTyr: 0.733 ± 0.182
0.0IleXaa: 0.0 ± 0.0
Lys
4.03LysAla: 4.03 ± 0.417
0.262LysCys: 0.262 ± 0.118
1.099LysAsp: 1.099 ± 0.242
0.471LysGlu: 0.471 ± 0.137
0.837LysPhe: 0.837 ± 0.25
1.57LysGly: 1.57 ± 0.307
0.471LysHis: 0.471 ± 0.168
1.256LysIle: 1.256 ± 0.223
0.314LysLys: 0.314 ± 0.118
2.46LysLeu: 2.46 ± 0.372
0.733LysMet: 0.733 ± 0.165
0.942LysAsn: 0.942 ± 0.244
1.466LysPro: 1.466 ± 0.236
0.995LysGln: 0.995 ± 0.278
2.303LysArg: 2.303 ± 0.324
1.413LysSer: 1.413 ± 0.334
1.518LysThr: 1.518 ± 0.291
2.094LysVal: 2.094 ± 0.326
0.314LysTrp: 0.314 ± 0.12
1.047LysTyr: 1.047 ± 0.226
0.0LysXaa: 0.0 ± 0.0
Leu
10.207LeuAla: 10.207 ± 0.696
0.628LeuCys: 0.628 ± 0.195
5.548LeuAsp: 5.548 ± 0.802
5.13LeuGlu: 5.13 ± 0.548
1.675LeuPhe: 1.675 ± 0.292
7.38LeuGly: 7.38 ± 0.92
1.361LeuHis: 1.361 ± 0.248
3.455LeuIle: 3.455 ± 0.467
2.041LeuLys: 2.041 ± 0.314
5.548LeuLeu: 5.548 ± 0.498
1.623LeuMet: 1.623 ± 0.206
2.041LeuAsn: 2.041 ± 0.346
5.077LeuPro: 5.077 ± 0.561
1.832LeuGln: 1.832 ± 0.454
5.025LeuArg: 5.025 ± 0.51
3.036LeuSer: 3.036 ± 0.392
5.496LeuThr: 5.496 ± 0.619
6.124LeuVal: 6.124 ± 0.622
1.518LeuTrp: 1.518 ± 0.321
1.466LeuTyr: 1.466 ± 0.246
0.0LeuXaa: 0.0 ± 0.0
Met
2.408MetAla: 2.408 ± 0.453
0.157MetCys: 0.157 ± 0.081
1.047MetAsp: 1.047 ± 0.213
0.366MetGlu: 0.366 ± 0.12
0.366MetPhe: 0.366 ± 0.13
1.309MetGly: 1.309 ± 0.277
0.366MetHis: 0.366 ± 0.134
1.152MetIle: 1.152 ± 0.234
0.523MetLys: 0.523 ± 0.164
1.78MetLeu: 1.78 ± 0.283
0.785MetMet: 0.785 ± 0.186
0.785MetAsn: 0.785 ± 0.194
2.251MetPro: 2.251 ± 0.298
0.576MetGln: 0.576 ± 0.162
2.251MetArg: 2.251 ± 0.373
1.518MetSer: 1.518 ± 0.328
3.35MetThr: 3.35 ± 0.397
1.518MetVal: 1.518 ± 0.312
0.523MetTrp: 0.523 ± 0.189
0.262MetTyr: 0.262 ± 0.121
0.0MetXaa: 0.0 ± 0.0
Asn
3.088AsnAla: 3.088 ± 0.478
0.157AsnCys: 0.157 ± 0.094
2.198AsnAsp: 2.198 ± 0.388
1.361AsnGlu: 1.361 ± 0.249
0.523AsnPhe: 0.523 ± 0.165
3.036AsnGly: 3.036 ± 0.414
0.68AsnHis: 0.68 ± 0.194
1.256AsnIle: 1.256 ± 0.329
0.68AsnLys: 0.68 ± 0.198
2.46AsnLeu: 2.46 ± 0.317
0.314AsnMet: 0.314 ± 0.146
0.837AsnAsn: 0.837 ± 0.225
2.303AsnPro: 2.303 ± 0.366
0.628AsnGln: 0.628 ± 0.181
1.57AsnArg: 1.57 ± 0.321
1.57AsnSer: 1.57 ± 0.318
1.78AsnThr: 1.78 ± 0.262
2.094AsnVal: 2.094 ± 0.386
0.68AsnTrp: 0.68 ± 0.182
0.419AsnTyr: 0.419 ± 0.131
0.0AsnXaa: 0.0 ± 0.0
Pro
7.799ProAla: 7.799 ± 0.671
0.733ProCys: 0.733 ± 0.229
6.281ProAsp: 6.281 ± 0.672
3.507ProGlu: 3.507 ± 0.523
1.57ProPhe: 1.57 ± 0.254
5.234ProGly: 5.234 ± 0.619
1.675ProHis: 1.675 ± 0.33
2.46ProIle: 2.46 ± 0.333
1.937ProLys: 1.937 ± 0.306
3.088ProLeu: 3.088 ± 0.414
1.518ProMet: 1.518 ± 0.275
2.041ProAsn: 2.041 ± 0.304
5.391ProPro: 5.391 ± 0.675
1.78ProGln: 1.78 ± 0.234
4.973ProArg: 4.973 ± 0.76
2.408ProSer: 2.408 ± 0.328
5.182ProThr: 5.182 ± 0.669
5.025ProVal: 5.025 ± 0.433
1.623ProTrp: 1.623 ± 0.378
1.413ProTyr: 1.413 ± 0.318
0.0ProXaa: 0.0 ± 0.0
Gln
4.711GlnAla: 4.711 ± 0.516
0.471GlnCys: 0.471 ± 0.155
1.256GlnAsp: 1.256 ± 0.306
1.099GlnGlu: 1.099 ± 0.241
1.152GlnPhe: 1.152 ± 0.256
1.78GlnGly: 1.78 ± 0.413
0.628GlnHis: 0.628 ± 0.159
1.832GlnIle: 1.832 ± 0.337
0.995GlnLys: 0.995 ± 0.202
4.03GlnLeu: 4.03 ± 0.426
1.413GlnMet: 1.413 ± 0.199
0.89GlnAsn: 0.89 ± 0.186
2.146GlnPro: 2.146 ± 0.232
2.512GlnGln: 2.512 ± 0.413
3.35GlnArg: 3.35 ± 0.463
1.78GlnSer: 1.78 ± 0.333
1.832GlnThr: 1.832 ± 0.307
3.664GlnVal: 3.664 ± 0.368
0.89GlnTrp: 0.89 ± 0.198
0.523GlnTyr: 0.523 ± 0.203
0.0GlnXaa: 0.0 ± 0.0
Arg
8.27ArgAla: 8.27 ± 0.773
0.995ArgCys: 0.995 ± 0.254
4.973ArgAsp: 4.973 ± 0.521
4.397ArgGlu: 4.397 ± 0.512
1.361ArgPhe: 1.361 ± 0.276
4.92ArgGly: 4.92 ± 0.586
1.937ArgHis: 1.937 ± 0.369
3.821ArgIle: 3.821 ± 0.423
2.931ArgLys: 2.931 ± 0.391
5.862ArgLeu: 5.862 ± 0.61
2.041ArgMet: 2.041 ± 0.351
2.251ArgAsn: 2.251 ± 0.309
3.664ArgPro: 3.664 ± 0.54
3.088ArgGln: 3.088 ± 0.4
8.061ArgArg: 8.061 ± 0.979
3.088ArgSer: 3.088 ± 0.401
4.554ArgThr: 4.554 ± 0.597
5.758ArgVal: 5.758 ± 0.628
1.466ArgTrp: 1.466 ± 0.292
1.937ArgTyr: 1.937 ± 0.342
0.0ArgXaa: 0.0 ± 0.0
Ser
6.124SerAla: 6.124 ± 0.851
0.262SerCys: 0.262 ± 0.148
2.617SerAsp: 2.617 ± 0.372
1.78SerGlu: 1.78 ± 0.3
0.837SerPhe: 0.837 ± 0.253
4.973SerGly: 4.973 ± 0.524
0.785SerHis: 0.785 ± 0.218
1.832SerIle: 1.832 ± 0.399
1.309SerLys: 1.309 ± 0.239
3.821SerLeu: 3.821 ± 0.475
1.309SerMet: 1.309 ± 0.253
0.942SerAsn: 0.942 ± 0.195
2.408SerPro: 2.408 ± 0.361
1.413SerGln: 1.413 ± 0.258
2.722SerArg: 2.722 ± 0.44
3.455SerSer: 3.455 ± 0.61
4.03SerThr: 4.03 ± 0.373
3.926SerVal: 3.926 ± 0.425
1.047SerTrp: 1.047 ± 0.233
0.942SerTyr: 0.942 ± 0.224
0.0SerXaa: 0.0 ± 0.0
Thr
8.322ThrAla: 8.322 ± 0.916
0.68ThrCys: 0.68 ± 0.187
5.287ThrAsp: 5.287 ± 0.689
4.501ThrGlu: 4.501 ± 0.655
2.041ThrPhe: 2.041 ± 0.298
6.229ThrGly: 6.229 ± 0.627
1.518ThrHis: 1.518 ± 0.313
3.559ThrIle: 3.559 ± 0.434
1.832ThrLys: 1.832 ± 0.266
4.658ThrLeu: 4.658 ± 0.607
1.518ThrMet: 1.518 ± 0.293
1.727ThrAsn: 1.727 ± 0.351
6.333ThrPro: 6.333 ± 0.635
1.413ThrGln: 1.413 ± 0.239
4.763ThrArg: 4.763 ± 0.592
3.35ThrSer: 3.35 ± 0.562
5.077ThrThr: 5.077 ± 0.555
5.077ThrVal: 5.077 ± 0.602
1.204ThrTrp: 1.204 ± 0.239
1.256ThrTyr: 1.256 ± 0.206
0.0ThrXaa: 0.0 ± 0.0
Val
9.422ValAla: 9.422 ± 0.764
0.576ValCys: 0.576 ± 0.233
6.333ValAsp: 6.333 ± 0.597
4.554ValGlu: 4.554 ± 0.462
1.937ValPhe: 1.937 ± 0.299
5.548ValGly: 5.548 ± 0.6
1.832ValHis: 1.832 ± 0.285
2.931ValIle: 2.931 ± 0.357
2.094ValLys: 2.094 ± 0.238
6.229ValLeu: 6.229 ± 0.516
1.361ValMet: 1.361 ± 0.253
1.518ValAsn: 1.518 ± 0.273
4.711ValPro: 4.711 ± 0.514
3.35ValGln: 3.35 ± 0.473
5.182ValArg: 5.182 ± 0.47
3.036ValSer: 3.036 ± 0.441
5.81ValThr: 5.81 ± 0.538
6.124ValVal: 6.124 ± 0.684
1.57ValTrp: 1.57 ± 0.316
1.989ValTyr: 1.989 ± 0.372
0.0ValXaa: 0.0 ± 0.0
Trp
2.355TrpAla: 2.355 ± 0.392
0.419TrpCys: 0.419 ± 0.143
1.309TrpAsp: 1.309 ± 0.255
0.68TrpGlu: 0.68 ± 0.18
0.733TrpPhe: 0.733 ± 0.193
1.099TrpGly: 1.099 ± 0.201
0.628TrpHis: 0.628 ± 0.187
0.628TrpIle: 0.628 ± 0.178
0.523TrpLys: 0.523 ± 0.152
2.355TrpLeu: 2.355 ± 0.389
0.523TrpMet: 0.523 ± 0.156
0.419TrpAsn: 0.419 ± 0.222
1.57TrpPro: 1.57 ± 0.254
1.099TrpGln: 1.099 ± 0.275
1.78TrpArg: 1.78 ± 0.335
1.57TrpSer: 1.57 ± 0.319
1.204TrpThr: 1.204 ± 0.251
1.466TrpVal: 1.466 ± 0.282
0.576TrpTrp: 0.576 ± 0.226
0.209TrpTyr: 0.209 ± 0.109
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.832TyrAla: 1.832 ± 0.31
0.105TyrCys: 0.105 ± 0.078
2.094TyrAsp: 2.094 ± 0.38
1.309TyrGlu: 1.309 ± 0.335
0.785TyrPhe: 0.785 ± 0.17
2.041TyrGly: 2.041 ± 0.21
0.419TyrHis: 0.419 ± 0.18
0.68TyrIle: 0.68 ± 0.154
0.471TyrLys: 0.471 ± 0.184
1.78TyrLeu: 1.78 ± 0.262
0.471TyrMet: 0.471 ± 0.145
0.576TyrAsn: 0.576 ± 0.159
1.466TyrPro: 1.466 ± 0.297
0.785TyrGln: 0.785 ± 0.198
1.727TyrArg: 1.727 ± 0.238
1.047TyrSer: 1.047 ± 0.231
1.518TyrThr: 1.518 ± 0.34
1.413TyrVal: 1.413 ± 0.305
0.471TyrTrp: 0.471 ± 0.142
0.628TyrTyr: 0.628 ± 0.17
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 87 proteins (19106 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski