Vibrio phage JSF14

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 217 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2D0YZ02|A0A2D0YZ02_9CAUD Uncharacterized protein OS=Vibrio phage JSF14 OX=1983597 PE=4 SV=1
MM1 pKa = 7.63LYY3 pKa = 10.5DD4 pKa = 3.49VYY6 pKa = 10.97RR7 pKa = 11.84VDD9 pKa = 4.42RR10 pKa = 11.84HH11 pKa = 5.55PHH13 pKa = 6.54DD14 pKa = 4.06IDD16 pKa = 5.12DD17 pKa = 4.27VVEE20 pKa = 4.19TCIGVDD26 pKa = 3.67LSDD29 pKa = 4.41MEE31 pKa = 4.61VKK33 pKa = 10.76DD34 pKa = 6.27LIATQNGYY42 pKa = 10.73LEE44 pKa = 4.28IFGVDD49 pKa = 3.55SEE51 pKa = 4.61GLVYY55 pKa = 10.91DD56 pKa = 4.35KK57 pKa = 11.4VGEE60 pKa = 4.06

Molecular weight: 6.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2D0Z5J6|A0A2D0Z5J6_9CAUD Uncharacterized protein OS=Vibrio phage JSF14 OX=1983597 PE=4 SV=1
MM1 pKa = 7.36QRR3 pKa = 11.84LLKK6 pKa = 9.4HH7 pKa = 4.63TEE9 pKa = 3.52KK10 pKa = 10.86RR11 pKa = 11.84IDD13 pKa = 3.29RR14 pKa = 11.84VLRR17 pKa = 11.84LMATGYY23 pKa = 7.9TQDD26 pKa = 3.71GKK28 pKa = 10.35HH29 pKa = 5.41LTRR32 pKa = 11.84SVTDD36 pKa = 3.51RR37 pKa = 11.84AEE39 pKa = 4.0EE40 pKa = 3.94LSTLKK45 pKa = 10.51RR46 pKa = 11.84VTFLRR51 pKa = 11.84GG52 pKa = 2.89

Molecular weight: 6.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

217

0

217

35814

37

1076

165.0

18.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.004 ± 0.212

1.751 ± 0.114

6.478 ± 0.121

7.757 ± 0.187

4.3 ± 0.113

6.361 ± 0.15

1.982 ± 0.078

6.344 ± 0.125

8.022 ± 0.19

8.466 ± 0.185

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.516 ± 0.104

5.283 ± 0.147

2.82 ± 0.1

3.415 ± 0.114

4.13 ± 0.143

6.503 ± 0.145

5.822 ± 0.193

6.849 ± 0.197

1.522 ± 0.076

4.677 ± 0.146
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski