Oceanimonas doudoroffii
Average proteome isoelectric point is 6.3
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3522 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A233RHX6|A0A233RHX6_9GAMM DAO domain-containing protein OS=Oceanimonas doudoroffii OX=84158 GN=B6S08_05710 PE=4 SV=1
MM1 pKa = 7.68 KK2 pKa = 9.38 KK3 pKa = 9.08 TILALTIPALFATSASAVTVYY24 pKa = 10.69 SDD26 pKa = 3.76 EE27 pKa = 4.34 GNQVDD32 pKa = 3.12 IYY34 pKa = 11.59 GRR36 pKa = 11.84 VQYY39 pKa = 10.28 EE40 pKa = 3.75 AGEE43 pKa = 4.25 RR44 pKa = 11.84 DD45 pKa = 3.75 YY46 pKa = 11.71 QSGGKK51 pKa = 9.76 AEE53 pKa = 4.15 NFGGDD58 pKa = 3.1 GEE60 pKa = 4.47 ARR62 pKa = 11.84 LGVNVKK68 pKa = 10.69 YY69 pKa = 9.76 MVNNDD74 pKa = 2.52 VDD76 pKa = 5.61 LIGKK80 pKa = 8.07 LEE82 pKa = 4.05 WAVVTEE88 pKa = 4.35 GDD90 pKa = 3.98 EE91 pKa = 4.27 EE92 pKa = 5.27 SDD94 pKa = 4.13 GVDD97 pKa = 3.31 DD98 pKa = 5.65 GEE100 pKa = 5.24 GISSRR105 pKa = 11.84 YY106 pKa = 8.4 AWAGFRR112 pKa = 11.84 FMDD115 pKa = 3.78 TTDD118 pKa = 3.27 LTFGRR123 pKa = 11.84 VNSPYY128 pKa = 10.98 AQLTDD133 pKa = 3.57 YY134 pKa = 11.22 TDD136 pKa = 3.23 VLNIFGGAVAFGDD149 pKa = 4.02 EE150 pKa = 5.06 LSTTDD155 pKa = 4.58 KK156 pKa = 10.92 VDD158 pKa = 3.35 DD159 pKa = 4.0 MVRR162 pKa = 11.84 LSYY165 pKa = 11.48 ANNGLDD171 pKa = 4.06 LRR173 pKa = 11.84 AGYY176 pKa = 10.62 AFNDD180 pKa = 3.32 NDD182 pKa = 5.15 KK183 pKa = 10.85 IDD185 pKa = 3.92 GNTGEE190 pKa = 4.11 EE191 pKa = 4.16 NRR193 pKa = 11.84 WNVSAGYY200 pKa = 7.03 TLPMGLGFGAAYY212 pKa = 9.37 EE213 pKa = 4.21 QISFLDD219 pKa = 5.78 AEE221 pKa = 4.98 DD222 pKa = 3.4 QDD224 pKa = 3.6 NWIVGVNYY232 pKa = 9.59 TLDD235 pKa = 3.74 GLYY238 pKa = 10.49 LAAIYY243 pKa = 8.23 TEE245 pKa = 4.36 RR246 pKa = 11.84 EE247 pKa = 3.44 QDD249 pKa = 3.51 VAGGTNEE256 pKa = 3.83 AEE258 pKa = 4.69 GYY260 pKa = 8.75 EE261 pKa = 3.98 LHH263 pKa = 7.42 AEE265 pKa = 4.23 YY266 pKa = 10.86 NVDD269 pKa = 2.92 AWKK272 pKa = 10.97 VLAQYY277 pKa = 10.91 SKK279 pKa = 11.27 EE280 pKa = 4.11 EE281 pKa = 3.84 YY282 pKa = 10.21 DD283 pKa = 3.69 AAGSTAEE290 pKa = 4.34 VDD292 pKa = 3.78 TIDD295 pKa = 5.52 AITLGVQYY303 pKa = 11.19 SLTAKK308 pKa = 9.94 AKK310 pKa = 10.44 LYY312 pKa = 10.44 GEE314 pKa = 4.38 YY315 pKa = 10.39 VISDD319 pKa = 3.61 AADD322 pKa = 3.48 EE323 pKa = 4.73 DD324 pKa = 4.16 DD325 pKa = 4.91 LYY327 pKa = 11.75 GVGIQYY333 pKa = 10.53 NFF335 pKa = 3.35
Molecular weight: 36.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.708
IPC2_protein 3.821
IPC_protein 3.834
Toseland 3.617
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.961
Wikipedia 3.732
Rodwell 3.656
Grimsley 3.516
Solomon 3.808
Lehninger 3.77
Nozaki 3.923
DTASelect 4.151
Thurlkill 3.656
EMBOSS 3.745
Sillero 3.948
Patrickios 1.049
IPC_peptide 3.808
IPC2_peptide 3.923
IPC2.peptide.svr19 3.837
Protein with the highest isoelectric point:
>tr|A0A233RAW8|A0A233RAW8_9GAMM Beta sliding clamp OS=Oceanimonas doudoroffii OX=84158 GN=B6S08_16625 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLKK11 pKa = 10.14 RR12 pKa = 11.84 KK13 pKa = 9.13 RR14 pKa = 11.84 SHH16 pKa = 6.17 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 NGRR28 pKa = 11.84 KK29 pKa = 9.16 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.29 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LSAA44 pKa = 3.91
Molecular weight: 5.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.676
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.398
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3522
0
3522
1139482
31
1611
323.5
35.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.644 ± 0.05
1.061 ± 0.012
5.209 ± 0.031
6.026 ± 0.034
3.701 ± 0.028
8.093 ± 0.032
2.475 ± 0.024
4.864 ± 0.036
3.307 ± 0.034
12.013 ± 0.061
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.642 ± 0.019
3.083 ± 0.029
4.657 ± 0.027
4.459 ± 0.033
6.416 ± 0.04
5.394 ± 0.026
4.848 ± 0.025
7.122 ± 0.033
1.448 ± 0.018
2.537 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here