Streptococcus satellite phage Javan305
Average proteome isoelectric point is 6.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZJ71|A0A4D5ZJ71_9VIRU Excalibur domain-containing protein OS=Streptococcus satellite phage Javan305 OX=2558625 GN=JavanS305_0008 PE=4 SV=1
MM1 pKa = 7.63 TEE3 pKa = 4.01 FNFDD7 pKa = 3.13 IDD9 pKa = 3.82 QIANNTVAKK18 pKa = 10.17 VDD20 pKa = 3.4 KK21 pKa = 7.97 TTQIIEE27 pKa = 4.46 TNTGSDD33 pKa = 2.99 KK34 pKa = 11.08 TLTFLEE40 pKa = 4.58 KK41 pKa = 10.55 LSPEE45 pKa = 3.85 QQEE48 pKa = 4.62 GIKK51 pKa = 10.52 AHH53 pKa = 5.93 VPQLVDD59 pKa = 4.1 QFVTNQNALLDD70 pKa = 4.38 FGQSAVEE77 pKa = 4.25 GVNNTVNRR85 pKa = 11.84 ILSEE89 pKa = 4.05 QKK91 pKa = 10.36 KK92 pKa = 9.48 LQIPQVDD99 pKa = 4.03 DD100 pKa = 3.72 LLKK103 pKa = 9.26 NTNRR107 pKa = 11.84 EE108 pKa = 3.93 LQGFVVKK115 pKa = 10.25 YY116 pKa = 9.81 KK117 pKa = 10.13 NAEE120 pKa = 3.74 IAEE123 pKa = 4.37 LEE125 pKa = 4.24 EE126 pKa = 4.85 KK127 pKa = 10.82 PNFLQRR133 pKa = 11.84 LFNKK137 pKa = 10.1 SKK139 pKa = 9.11 NTLQEE144 pKa = 4.4 FYY146 pKa = 10.5 FDD148 pKa = 3.9 SKK150 pKa = 10.03 TVEE153 pKa = 3.97 QKK155 pKa = 11.03 LDD157 pKa = 3.19 GMAAAVVKK165 pKa = 10.9 QEE167 pKa = 4.6 DD168 pKa = 3.74 VLARR172 pKa = 11.84 NIVSAEE178 pKa = 3.67 MLIEE182 pKa = 5.37 DD183 pKa = 3.67 NTKK186 pKa = 10.67 SIEE189 pKa = 4.06 NLVGVISFIEE199 pKa = 4.27 ASQTEE204 pKa = 4.32 AGNRR208 pKa = 11.84 ATEE211 pKa = 4.45 LKK213 pKa = 10.61 AQADD217 pKa = 3.86 QLDD220 pKa = 4.2 TSTVEE225 pKa = 4.17 YY226 pKa = 8.76 QTKK229 pKa = 9.32 SQEE232 pKa = 4.07 LARR235 pKa = 11.84 MAEE238 pKa = 4.18 VVNTLEE244 pKa = 4.2 QQHH247 pKa = 5.54 TEE249 pKa = 4.22 YY250 pKa = 11.1 VSRR253 pKa = 11.84 LYY255 pKa = 10.39 VAWATTPQMRR265 pKa = 11.84 NLVKK269 pKa = 10.62 VSSDD273 pKa = 2.97 MRR275 pKa = 11.84 QKK277 pKa = 11.16 LGMLRR282 pKa = 11.84 RR283 pKa = 11.84 NTIPTMKK290 pKa = 10.54 LSIAQLGILQQSMKK304 pKa = 10.45 SGVVADD310 pKa = 5.39 AIVNANNAALQMLAEE325 pKa = 4.34 TSKK328 pKa = 11.04 EE329 pKa = 4.07 VIPQMEE335 pKa = 5.32 RR336 pKa = 11.84 IAQSPTVAVEE346 pKa = 4.27 SVTQLAEE353 pKa = 3.99 SLVAQNQGIVAAIEE367 pKa = 3.82 LGRR370 pKa = 11.84 QKK372 pKa = 10.42 RR373 pKa = 11.84 AQLEE377 pKa = 4.28 TTIVKK382 pKa = 9.44 SAEE385 pKa = 4.12 MINDD389 pKa = 3.62 SVKK392 pKa = 10.67 LRR394 pKa = 11.84 DD395 pKa = 3.71 EE396 pKa = 5.1 KK397 pKa = 10.74 IVQALLDD404 pKa = 3.69 QGKK407 pKa = 8.96 AAQKK411 pKa = 9.8 EE412 pKa = 4.34 VQEE415 pKa = 4.29
Molecular weight: 46.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.117
IPC2_protein 5.067
IPC_protein 4.978
Toseland 4.851
ProMoST 5.003
Dawson 4.902
Bjellqvist 5.067
Wikipedia 4.736
Rodwell 4.825
Grimsley 4.762
Solomon 4.902
Lehninger 4.851
Nozaki 5.003
DTASelect 5.118
Thurlkill 4.825
EMBOSS 4.762
Sillero 5.08
Patrickios 4.609
IPC_peptide 4.914
IPC2_peptide 5.08
IPC2.peptide.svr19 5.085
Protein with the highest isoelectric point:
>tr|A0A4D5ZJ41|A0A4D5ZJ41_9VIRU Putative toxic anion resistance protein OS=Streptococcus satellite phage Javan305 OX=2558625 GN=JavanS305_0011 PE=4 SV=1
MM1 pKa = 7.65 KK2 pKa = 9.94 IAEE5 pKa = 4.22 IKK7 pKa = 9.09 KK8 pKa = 9.16 QNGSTVYY15 pKa = 10.39 RR16 pKa = 11.84 SRR18 pKa = 11.84 VYY20 pKa = 10.84 LGIDD24 pKa = 2.94 MTTGKK29 pKa = 10.37 RR30 pKa = 11.84 IQANLTARR38 pKa = 11.84 TKK40 pKa = 10.67 TEE42 pKa = 4.16 LKK44 pKa = 10.56 KK45 pKa = 10.53 KK46 pKa = 10.04 AQLAKK51 pKa = 10.9 AEE53 pKa = 4.16 FQNNGCTKK61 pKa = 10.48 KK62 pKa = 9.28 EE63 pKa = 4.32 TISLTSYY70 pKa = 11.24 EE71 pKa = 4.31 EE72 pKa = 4.38 VAQLWWEE79 pKa = 4.57 SYY81 pKa = 7.5 QHH83 pKa = 5.01 TVKK86 pKa = 10.72 PNTQDD91 pKa = 3.01 SVKK94 pKa = 10.42 RR95 pKa = 11.84 LLANHH100 pKa = 6.83 VIPLFGSYY108 pKa = 10.42 RR109 pKa = 11.84 LDD111 pKa = 3.34 KK112 pKa = 10.39 LTTPTIQRR120 pKa = 11.84 IVNQLALRR128 pKa = 11.84 ANKK131 pKa = 10.05 RR132 pKa = 11.84 EE133 pKa = 3.95 EE134 pKa = 4.26 GAFLHH139 pKa = 6.3 YY140 pKa = 10.87 DD141 pKa = 4.12 KK142 pKa = 11.05 IHH144 pKa = 6.59 ALNKK148 pKa = 10.22 RR149 pKa = 11.84 ILQYY153 pKa = 11.19 AVTMQIIDD161 pKa = 3.61 INPARR166 pKa = 11.84 EE167 pKa = 3.9 VILPRR172 pKa = 11.84 KK173 pKa = 8.41 IKK175 pKa = 10.35 KK176 pKa = 9.35 GRR178 pKa = 11.84 NKK180 pKa = 9.77 VKK182 pKa = 10.46 HH183 pKa = 5.64 FNNLEE188 pKa = 3.99 LKK190 pKa = 10.51 QFLSYY195 pKa = 11.05 LEE197 pKa = 4.12 EE198 pKa = 4.98 ANLAIYY204 pKa = 10.3 RR205 pKa = 11.84 NIYY208 pKa = 10.05 EE209 pKa = 3.68 ITLYY213 pKa = 10.92 KK214 pKa = 10.38 FLLATGCRR222 pKa = 11.84 INEE225 pKa = 3.92 ALALHH230 pKa = 6.77 WSDD233 pKa = 4.46 IDD235 pKa = 3.63 LHH237 pKa = 6.01 NATVNITKK245 pKa = 9.15 TLNHH249 pKa = 6.65 LGEE252 pKa = 4.45 INSPKK257 pKa = 10.27 SEE259 pKa = 3.73 ASYY262 pKa = 8.95 RR263 pKa = 11.84 TIDD266 pKa = 4.0 IDD268 pKa = 3.64 PQTIEE273 pKa = 3.99 VLKK276 pKa = 10.61 VYY278 pKa = 9.35 QKK280 pKa = 10.51 RR281 pKa = 11.84 QRR283 pKa = 11.84 QEE285 pKa = 2.93 AWKK288 pKa = 10.19 LGRR291 pKa = 11.84 TEE293 pKa = 4.07 TVVFSDD299 pKa = 6.26 FIHH302 pKa = 7.25 KK303 pKa = 10.29 YY304 pKa = 9.53 PNNKK308 pKa = 8.18 TLSTRR313 pKa = 11.84 LNTRR317 pKa = 11.84 FKK319 pKa = 10.85 HH320 pKa = 6.0 AGVPNIGFHH329 pKa = 6.22 GFRR332 pKa = 11.84 HH333 pKa = 4.87 THH335 pKa = 6.58 ASLLLNSGIPYY346 pKa = 10.24 KK347 pKa = 10.53 KK348 pKa = 9.14 LQHH351 pKa = 6.53 RR352 pKa = 11.84 LGHH355 pKa = 5.57 STLAMTMDD363 pKa = 4.54 TYY365 pKa = 11.85 SHH367 pKa = 7.08 LSKK370 pKa = 10.76 EE371 pKa = 4.21 KK372 pKa = 10.8 AKK374 pKa = 9.65 TAVSFYY380 pKa = 9.94 EE381 pKa = 4.13 KK382 pKa = 10.49 AVSSLL387 pKa = 3.39
Molecular weight: 44.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.108
IPC2_protein 9.385
IPC_protein 9.326
Toseland 10.101
ProMoST 9.736
Dawson 10.277
Bjellqvist 9.926
Wikipedia 10.423
Rodwell 10.818
Grimsley 10.35
Solomon 10.292
Lehninger 10.262
Nozaki 10.087
DTASelect 9.911
Thurlkill 10.145
EMBOSS 10.496
Sillero 10.189
Patrickios 10.423
IPC_peptide 10.306
IPC2_peptide 8.419
IPC2.peptide.svr19 8.395
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
13
0
13
3397
56
513
261.3
29.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.035 ± 0.601
0.648 ± 0.179
5.328 ± 0.527
8.331 ± 0.738
3.209 ± 0.387
4.268 ± 0.302
1.913 ± 0.42
8.36 ± 0.622
9.361 ± 0.667
8.979 ± 0.734
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.855 ± 0.23
5.475 ± 0.39
2.679 ± 0.272
4.475 ± 0.863
3.945 ± 0.432
8.184 ± 0.779
6.094 ± 0.917
5.946 ± 0.545
0.883 ± 0.182
4.033 ± 0.531
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here