Monilinia laxa

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Helotiales; Sclerotiniaceae; Monilinia

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12416 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5N6KG37|A0A5N6KG37_9HELO Uncharacterized protein OS=Monilinia laxa OX=61186 GN=EYC80_006093 PE=3 SV=1
MM1 pKa = 7.15VLPVFVFLPLYY12 pKa = 8.13MYY14 pKa = 9.7PYY16 pKa = 10.98NSTSWSTLTTSIVSNPNLKK35 pKa = 9.85FHH37 pKa = 6.44VVVSPNLAFTDD48 pKa = 3.5PDD50 pKa = 3.57ANYY53 pKa = 10.32VAGISILKK61 pKa = 9.61SHH63 pKa = 6.93ANVLTLGYY71 pKa = 10.5VYY73 pKa = 10.4TSYY76 pKa = 11.64GLRR79 pKa = 11.84DD80 pKa = 3.42ISEE83 pKa = 3.98IEE85 pKa = 3.67ADD87 pKa = 3.1INKK90 pKa = 9.45YY91 pKa = 10.4VGWNKK96 pKa = 9.57PAEE99 pKa = 4.16IPVDD103 pKa = 4.09GIFFDD108 pKa = 4.63EE109 pKa = 4.55SPSNNSTFSYY119 pKa = 10.1MSTLSSYY126 pKa = 11.73AKK128 pKa = 9.77TSLGAGKK135 pKa = 10.09DD136 pKa = 3.83YY137 pKa = 11.21IFLNPGVPVDD147 pKa = 3.51ASFYY151 pKa = 10.46TLADD155 pKa = 3.88SINVFEE161 pKa = 4.28EE162 pKa = 4.89SYY164 pKa = 11.87AKK166 pKa = 10.44FNKK169 pKa = 8.87TALNSLDD176 pKa = 3.42KK177 pKa = 10.8TLLAKK182 pKa = 9.18STYY185 pKa = 10.11AIHH188 pKa = 7.3DD189 pKa = 3.88FKK191 pKa = 11.33GTVAQQIDD199 pKa = 5.48LISQLASANLAGILITTQPGYY220 pKa = 10.98AVWSSLWSSFCSAVSRR236 pKa = 11.84LNLSGRR242 pKa = 11.84IGEE245 pKa = 4.51GGGNGDD251 pKa = 4.87GIGNDD256 pKa = 3.5GGYY259 pKa = 10.94GGGDD263 pKa = 4.2DD264 pKa = 6.02RR265 pKa = 11.84DD266 pKa = 4.82DD267 pKa = 6.41DD268 pKa = 6.57DD269 pKa = 7.77DD270 pKa = 7.58DD271 pKa = 7.55DD272 pKa = 6.71DD273 pKa = 6.2DD274 pKa = 6.0NSGNDD279 pKa = 4.11DD280 pKa = 4.79DD281 pKa = 6.74DD282 pKa = 7.21DD283 pKa = 6.59GDD285 pKa = 3.9EE286 pKa = 4.21EE287 pKa = 4.62TT288 pKa = 5.14

Molecular weight: 31.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5N6JNB2|A0A5N6JNB2_9HELO Uncharacterized protein OS=Monilinia laxa OX=61186 GN=EYC80_010279 PE=4 SV=1
MM1 pKa = 7.19LQKK4 pKa = 10.75KK5 pKa = 9.45HH6 pKa = 6.49LFTNNFLVSTQNISTKK22 pKa = 10.6SPTKK26 pKa = 10.47HH27 pKa = 5.18STHH30 pKa = 7.16PKK32 pKa = 10.47LKK34 pKa = 8.44MQTKK38 pKa = 9.98RR39 pKa = 11.84LVLNHH44 pKa = 6.29SLNLRR49 pKa = 11.84LPVFSTRR56 pKa = 11.84KK57 pKa = 9.34PKK59 pKa = 10.09ILRR62 pKa = 11.84SGTSQVWTT70 pKa = 4.02

Molecular weight: 8.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11157

1259

12416

5571812

66

7051

448.8

49.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.598 ± 0.021

1.164 ± 0.009

5.479 ± 0.016

6.466 ± 0.028

3.754 ± 0.013

6.784 ± 0.022

2.35 ± 0.01

5.543 ± 0.014

5.46 ± 0.022

8.554 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.201 ± 0.01

4.269 ± 0.015

5.903 ± 0.023

3.925 ± 0.018

5.703 ± 0.019

8.858 ± 0.031

6.142 ± 0.019

5.654 ± 0.014

1.346 ± 0.008

2.844 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski