Sporosarcina phage Lietuvens

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 100 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G6XUT8|A0A6G6XUT8_9CAUD Uncharacterized protein OS=Sporosarcina phage Lietuvens OX=2713145 PE=4 SV=1
MM1 pKa = 7.4TCNEE5 pKa = 4.42CNADD9 pKa = 4.07LNAADD14 pKa = 4.16PHH16 pKa = 6.17TDD18 pKa = 2.81ATYY21 pKa = 10.97DD22 pKa = 3.38DD23 pKa = 4.54HH24 pKa = 8.15AQVYY28 pKa = 8.85YY29 pKa = 11.01CDD31 pKa = 3.82VDD33 pKa = 4.6CFRR36 pKa = 11.84AWADD40 pKa = 3.24GHH42 pKa = 6.08FDD44 pKa = 3.5EE45 pKa = 5.12VTAYY49 pKa = 9.18YY50 pKa = 11.01EE51 pKa = 4.08RR52 pKa = 11.84MNVQQ56 pKa = 2.89

Molecular weight: 6.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G6XVM5|A0A6G6XVM5_9CAUD Site-specific integrase OS=Sporosarcina phage Lietuvens OX=2713145 PE=3 SV=1
MM1 pKa = 7.23IVYY4 pKa = 9.28VYY6 pKa = 10.71RR7 pKa = 11.84LAFYY11 pKa = 10.67IEE13 pKa = 4.0IAGLNLSKK21 pKa = 10.38EE22 pKa = 4.34RR23 pKa = 11.84KK24 pKa = 7.84ATRR27 pKa = 11.84QKK29 pKa = 10.89KK30 pKa = 8.5EE31 pKa = 3.79RR32 pKa = 11.84QPAPRR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84YY40 pKa = 9.34

Molecular weight: 4.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

100

0

100

19522

30

914

195.2

21.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.241 ± 0.426

0.85 ± 0.138

6.628 ± 0.221

7.228 ± 0.32

3.611 ± 0.185

7.064 ± 0.312

1.834 ± 0.107

5.983 ± 0.201

6.787 ± 0.244

7.812 ± 0.216

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.418 ± 0.151

4.211 ± 0.225

3.237 ± 0.153

2.848 ± 0.149

5.855 ± 0.28

5.793 ± 0.192

6.342 ± 0.204

7.079 ± 0.23

1.101 ± 0.084

4.077 ± 0.162
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski