Cenarchaeum symbiosum (strain A)
Average proteome isoelectric point is 6.73
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2022 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0RY25|A0RY25_CENSY Uncharacterized protein OS=Cenarchaeum symbiosum (strain A) OX=414004 GN=CENSYa_1624 PE=4 SV=1
MM1 pKa = 7.45 NKK3 pKa = 9.93 GIYY6 pKa = 6.98 MTGGLAAVLFTLALTPAGASFLPAGQAASPLFYY39 pKa = 11.19 GMAEE43 pKa = 4.16 LVHH46 pKa = 7.77 IDD48 pKa = 3.35 GDD50 pKa = 4.08 GGIVSSQTVHH60 pKa = 6.56 NRR62 pKa = 11.84 LLDD65 pKa = 3.62 TGEE68 pKa = 4.74 GILIKK73 pKa = 10.78 GIFGTLDD80 pKa = 2.8 TGEE83 pKa = 4.46 APRR86 pKa = 11.84 LICITDD92 pKa = 3.67 DD93 pKa = 3.73 PGILITNNTDD103 pKa = 3.29 TPDD106 pKa = 3.58 EE107 pKa = 4.26 EE108 pKa = 4.73 TLLSADD114 pKa = 4.44 FDD116 pKa = 4.64 PGSGFANSTISTCKK130 pKa = 9.55 MADD133 pKa = 2.89 IDD135 pKa = 4.07 TSTDD139 pKa = 2.56 EE140 pKa = 4.32 SMAVMTATFNGSASSTPNILAGQEE164 pKa = 3.69 ITHH167 pKa = 6.82 LVICNQDD174 pKa = 3.08 TDD176 pKa = 3.66 SSAFTRR182 pKa = 11.84 CSAAGDD188 pKa = 3.42 AFAAINVGITTVASDD203 pKa = 3.91 DD204 pKa = 4.25 SVLVTYY210 pKa = 9.35 TYY212 pKa = 11.57 DD213 pKa = 3.29 LTSPDD218 pKa = 3.24 TT219 pKa = 3.81
Molecular weight: 22.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.691
IPC2_protein 3.681
IPC_protein 3.694
Toseland 3.465
ProMoST 3.884
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.681
Rodwell 3.516
Grimsley 3.376
Solomon 3.694
Lehninger 3.656
Nozaki 3.821
DTASelect 4.101
Thurlkill 3.528
EMBOSS 3.681
Sillero 3.821
Patrickios 0.998
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.755
Protein with the highest isoelectric point:
>tr|A0RUH0|A0RUH0_CENSY Uncharacterized protein OS=Cenarchaeum symbiosum (strain A) OX=414004 GN=CENSYa_0351 PE=4 SV=1
MM1 pKa = 7.24 KK2 pKa = 10.24 RR3 pKa = 11.84 KK4 pKa = 9.24 AVKK7 pKa = 8.83 KK8 pKa = 7.84 TATRR12 pKa = 11.84 RR13 pKa = 11.84 KK14 pKa = 10.0 APGRR18 pKa = 11.84 KK19 pKa = 8.75 PGPKK23 pKa = 9.41 KK24 pKa = 10.76 GSTKK28 pKa = 9.81 RR29 pKa = 11.84 RR30 pKa = 11.84 KK31 pKa = 9.84 SPGRR35 pKa = 11.84 KK36 pKa = 7.49 PAGRR40 pKa = 11.84 KK41 pKa = 7.35 PAGRR45 pKa = 11.84 KK46 pKa = 8.07 PAAKK50 pKa = 10.0 KK51 pKa = 8.63 GAKK54 pKa = 9.06 RR55 pKa = 11.84 RR56 pKa = 11.84 KK57 pKa = 9.32 SPGRR61 pKa = 11.84 KK62 pKa = 7.49 PAGRR66 pKa = 11.84 KK67 pKa = 7.35 PAGRR71 pKa = 11.84 KK72 pKa = 8.07 PAAKK76 pKa = 10.0 KK77 pKa = 8.63 GAKK80 pKa = 9.06 RR81 pKa = 11.84 RR82 pKa = 11.84 KK83 pKa = 9.32 SPGRR87 pKa = 11.84 KK88 pKa = 7.49 PAGRR92 pKa = 11.84 KK93 pKa = 7.35 PAGRR97 pKa = 11.84 KK98 pKa = 7.96 PAAKK102 pKa = 10.04 KK103 pKa = 9.34 GVKK106 pKa = 9.31 RR107 pKa = 11.84 RR108 pKa = 11.84 KK109 pKa = 5.16 TTKK112 pKa = 8.89 KK113 pKa = 7.75 TAAKK117 pKa = 9.88 RR118 pKa = 11.84 KK119 pKa = 8.12 VRR121 pKa = 11.84 RR122 pKa = 3.6
Molecular weight: 13.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.416
IPC2_protein 11.652
IPC_protein 13.203
Toseland 13.378
ProMoST 13.861
Dawson 13.378
Bjellqvist 13.364
Wikipedia 13.846
Rodwell 13.261
Grimsley 13.422
Solomon 13.876
Lehninger 13.773
Nozaki 13.378
DTASelect 13.364
Thurlkill 13.378
EMBOSS 13.876
Sillero 13.378
Patrickios 12.969
IPC_peptide 13.876
IPC2_peptide 12.852
IPC2.peptide.svr19 9.462
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2022
0
2022
623813
29
11910
308.5
33.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.235 ± 0.089
1.151 ± 0.045
6.513 ± 0.104
6.278 ± 0.089
3.54 ± 0.038
9.554 ± 0.104
2.083 ± 0.03
5.892 ± 0.063
4.164 ± 0.158
8.244 ± 0.061
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.97 ± 0.042
2.956 ± 0.05
5.225 ± 0.069
2.384 ± 0.031
6.374 ± 0.086
7.094 ± 0.101
5.271 ± 0.062
7.111 ± 0.049
0.857 ± 0.03
3.09 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here