Furcraea necrotic streak virus (isolate/Colombia/Furcraea/Cauca/2004)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Procedovirinae; Macanavirus; Furcraea necrotic streak virus

Average proteome isoelectric point is 7.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D3U5B8|D3U5B8_FNSVC Capsid protein OS=Furcraea necrotic streak virus (isolate/Colombia/Furcraea/Cauca/2004) OX=1554495 GN=gp5 PE=3 SV=1
MM1 pKa = 8.03KK2 pKa = 10.21CRR4 pKa = 11.84CCDD7 pKa = 3.53NEE9 pKa = 4.29PTLSPLFPFFAILVLVLAILVVGTPHH35 pKa = 6.05VQYY38 pKa = 10.97QSDD41 pKa = 3.48QHH43 pKa = 7.9HH44 pKa = 6.59NEE46 pKa = 4.07SKK48 pKa = 10.05IQHH51 pKa = 5.13ITIGKK56 pKa = 8.91

Molecular weight: 6.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D3U5B7|D3U5B7_FNSVC Movement protein OS=Furcraea necrotic streak virus (isolate/Colombia/Furcraea/Cauca/2004) OX=1554495 GN=gp4 PE=4 SV=1
MM1 pKa = 7.43VSHH4 pKa = 6.48QISRR8 pKa = 11.84KK9 pKa = 8.95LLSSGMTTYY18 pKa = 10.86KK19 pKa = 9.7WISVTRR25 pKa = 11.84SPVIRR30 pKa = 11.84ANVSKK35 pKa = 10.86ALFYY39 pKa = 10.32MEE41 pKa = 5.45YY42 pKa = 10.34NIQSDD47 pKa = 3.93GEE49 pKa = 4.22RR50 pKa = 11.84VVRR53 pKa = 11.84TRR55 pKa = 11.84RR56 pKa = 11.84PVKK59 pKa = 10.76DD60 pKa = 3.73RR61 pKa = 11.84DD62 pKa = 3.65NKK64 pKa = 10.5LSGVKK69 pKa = 9.34EE70 pKa = 3.84QAVGQTSQKK79 pKa = 9.87SHH81 pKa = 5.88QGQGNGVMTNIAEE94 pKa = 4.42TQTITVTYY102 pKa = 10.27NFNFF106 pKa = 3.71

Molecular weight: 12.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1509

56

762

301.8

33.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.958 ± 0.741

2.187 ± 0.318

5.103 ± 0.486

5.633 ± 1.279

4.705 ± 0.355

6.693 ± 0.512

2.187 ± 0.433

5.302 ± 0.322

5.103 ± 0.429

7.753 ± 0.725

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.253 ± 0.258

4.44 ± 0.503

5.302 ± 0.503

3.777 ± 0.625

7.886 ± 1.296

5.964 ± 1.612

6.229 ± 1.427

8.151 ± 0.637

1.325 ± 0.14

2.85 ± 0.373
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski