Wuhan house centipede virus 4
Average proteome isoelectric point is 7.11
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KF33|A0A1L3KF33_9VIRU Capsid protein OS=Wuhan house centipede virus 4 OX=1923708 PE=3 SV=1
MM1 pKa = 7.55 YY2 pKa = 10.35 PMEE5 pKa = 5.34 DD6 pKa = 3.36 FGDD9 pKa = 3.8 TFSARR14 pKa = 11.84 LYY16 pKa = 10.17 AGVRR20 pKa = 11.84 HH21 pKa = 5.59 VMYY24 pKa = 10.43 DD25 pKa = 3.13 LSASLRR31 pKa = 11.84 GEE33 pKa = 3.6 LRR35 pKa = 11.84 PRR37 pKa = 11.84 VWLALAAFSFLLEE50 pKa = 4.81 GIFMAYY56 pKa = 9.54 GQGGPVSLAIHH67 pKa = 5.85 ILGWIGTVWSWVGRR81 pKa = 11.84 WWRR84 pKa = 11.84 RR85 pKa = 11.84 AQIKK89 pKa = 9.07 WEE91 pKa = 4.02 QEE93 pKa = 3.44 QHH95 pKa = 6.1 GYY97 pKa = 8.18 RR98 pKa = 11.84 TMPEE102 pKa = 4.08 SVRR105 pKa = 11.84 EE106 pKa = 4.06 GSPLLNVTRR115 pKa = 11.84 PNFVVGVFALYY126 pKa = 10.03 KK127 pKa = 10.16 GKK129 pKa = 10.63 KK130 pKa = 8.79 ILLGTAWRR138 pKa = 11.84 YY139 pKa = 9.55 RR140 pKa = 11.84 DD141 pKa = 3.73 NLITAAHH148 pKa = 5.63 VVMEE152 pKa = 4.43 KK153 pKa = 10.68 NFDD156 pKa = 3.91 KK157 pKa = 10.96 ILIHH161 pKa = 6.54 IAKK164 pKa = 8.87 TDD166 pKa = 3.41 VYY168 pKa = 11.4 YY169 pKa = 10.06 EE170 pKa = 4.22 APDD173 pKa = 3.39 WKK175 pKa = 10.73 HH176 pKa = 5.12 VVGDD180 pKa = 3.81 VAIHH184 pKa = 6.32 PMQATWPIPSAKK196 pKa = 8.99 IDD198 pKa = 4.09 TIAKK202 pKa = 9.19 SAFVMAFSARR212 pKa = 11.84 DD213 pKa = 3.45 TSNASSGLLKK223 pKa = 10.54 HH224 pKa = 6.37 KK225 pKa = 9.24 PAKK228 pKa = 10.76 AMGFLQYY235 pKa = 10.42 EE236 pKa = 5.14 GSTLPGFSGSPYY248 pKa = 8.7 FTGTKK253 pKa = 9.46 VLGMHH258 pKa = 6.85 IGGGSAGNFGFSASFIEE275 pKa = 4.27 MMIKK279 pKa = 9.86 SRR281 pKa = 11.84 RR282 pKa = 11.84 RR283 pKa = 11.84 PEE285 pKa = 4.05 SSDD288 pKa = 3.32 LEE290 pKa = 4.18 MVKK293 pKa = 10.25 RR294 pKa = 11.84 VLRR297 pKa = 11.84 TTRR300 pKa = 11.84 VVDD303 pKa = 4.03 FDD305 pKa = 4.27 YY306 pKa = 11.4 EE307 pKa = 4.08 QGLDD311 pKa = 3.32 EE312 pKa = 4.11 TRR314 pKa = 11.84 IRR316 pKa = 11.84 IDD318 pKa = 2.82 GRR320 pKa = 11.84 YY321 pKa = 9.05 VEE323 pKa = 4.85 LPNEE327 pKa = 4.28 EE328 pKa = 4.04 FWEE331 pKa = 4.35 LFDD334 pKa = 6.08 DD335 pKa = 5.79 EE336 pKa = 5.11 EE337 pKa = 5.79 FEE339 pKa = 4.89 DD340 pKa = 4.17 YY341 pKa = 10.44 FYY343 pKa = 11.22 EE344 pKa = 4.63 FEE346 pKa = 6.16 DD347 pKa = 5.22 GSLHH351 pKa = 6.01 QNRR354 pKa = 11.84 NKK356 pKa = 10.33 KK357 pKa = 9.7 KK358 pKa = 9.32 KK359 pKa = 9.6 KK360 pKa = 8.93 QYY362 pKa = 10.62 NWDD365 pKa = 3.83 AEE367 pKa = 4.22 PDD369 pKa = 3.58 LGDD372 pKa = 4.09 DD373 pKa = 4.41 YY374 pKa = 11.46 EE375 pKa = 4.81 PEE377 pKa = 4.06 SAIRR381 pKa = 11.84 EE382 pKa = 4.2 PVFQEE387 pKa = 4.27 VPPSGIPTGVEE398 pKa = 3.86 DD399 pKa = 4.82 QVTEE403 pKa = 4.15 LRR405 pKa = 11.84 NMHH408 pKa = 6.36 TSLMTSLEE416 pKa = 3.93 GLEE419 pKa = 4.15 TRR421 pKa = 11.84 ISSGQQEE428 pKa = 4.52 MKK430 pKa = 10.88 DD431 pKa = 3.3 NLRR434 pKa = 11.84 EE435 pKa = 4.16 VFTSMLADD443 pKa = 3.19 EE444 pKa = 4.65 RR445 pKa = 11.84 DD446 pKa = 3.64 RR447 pKa = 11.84 YY448 pKa = 10.98 EE449 pKa = 4.6 GMLNQVEE456 pKa = 4.54 TKK458 pKa = 10.58 LIQLSGTCYY467 pKa = 10.43 KK468 pKa = 10.48 QIQSDD473 pKa = 3.9 LDD475 pKa = 3.7 DD476 pKa = 5.02 RR477 pKa = 11.84 ICRR480 pKa = 11.84 VMQSSPIISTTSCAPSTPKK499 pKa = 10.47 AQQDD503 pKa = 3.94 SVTSKK508 pKa = 8.77 NTRR511 pKa = 11.84 PLVTHH516 pKa = 5.83 WVGMEE521 pKa = 3.89 SDD523 pKa = 3.66 LQKK526 pKa = 11.01 FEE528 pKa = 3.82 TWRR531 pKa = 11.84 NSVDD535 pKa = 3.31 VSRR538 pKa = 11.84 SDD540 pKa = 3.78 YY541 pKa = 11.21 VVLRR545 pKa = 11.84 DD546 pKa = 3.29 KK547 pKa = 10.9 FLRR550 pKa = 11.84 DD551 pKa = 3.47 GLSLNISQAEE561 pKa = 3.82 ALIARR566 pKa = 11.84 FRR568 pKa = 11.84 NRR570 pKa = 11.84 QRR572 pKa = 11.84 TTKK575 pKa = 10.32 RR576 pKa = 11.84 RR577 pKa = 11.84 VKK579 pKa = 10.53 KK580 pKa = 7.9 PTPPTQQ586 pKa = 3.1
Molecular weight: 66.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.213
IPC2_protein 6.135
IPC_protein 6.237
Toseland 6.649
ProMoST 6.547
Dawson 6.532
Bjellqvist 6.504
Wikipedia 6.547
Rodwell 6.517
Grimsley 6.81
Solomon 6.532
Lehninger 6.517
Nozaki 6.781
DTASelect 6.971
Thurlkill 7.0
EMBOSS 6.971
Sillero 6.898
Patrickios 4.507
IPC_peptide 6.547
IPC2_peptide 6.737
IPC2.peptide.svr19 6.795
Protein with the highest isoelectric point:
>tr|A0A1L3KF33|A0A1L3KF33_9VIRU Capsid protein OS=Wuhan house centipede virus 4 OX=1923708 PE=3 SV=1
MM1 pKa = 6.95 STAEE5 pKa = 3.76 QLRR8 pKa = 11.84 RR9 pKa = 11.84 EE10 pKa = 4.26 LQALTDD16 pKa = 3.49 KK17 pKa = 10.78 MNNLSVAKK25 pKa = 9.77 KK26 pKa = 9.75 RR27 pKa = 11.84 KK28 pKa = 8.16 RR29 pKa = 11.84 SRR31 pKa = 11.84 RR32 pKa = 11.84 KK33 pKa = 7.92 KK34 pKa = 7.85 TSGIAPVPGTSAVAQVQSLGKK55 pKa = 9.14 KK56 pKa = 8.03 ARR58 pKa = 11.84 RR59 pKa = 11.84 RR60 pKa = 11.84 RR61 pKa = 11.84 GQPSAVGEE69 pKa = 4.3 GEE71 pKa = 3.94 IVFSRR76 pKa = 11.84 EE77 pKa = 3.4 EE78 pKa = 4.37 LIRR81 pKa = 11.84 TLTLPVGKK89 pKa = 8.89 ATVTDD94 pKa = 4.43 YY95 pKa = 11.55 IDD97 pKa = 4.37 IIPVNFRR104 pKa = 11.84 FLKK107 pKa = 10.66 SIGAVFEE114 pKa = 4.69 RR115 pKa = 11.84 IQWLSLQVYY124 pKa = 7.26 WKK126 pKa = 9.15 PAVGTTYY133 pKa = 10.97 GGLVTYY139 pKa = 10.36 GVDD142 pKa = 2.94 WDD144 pKa = 4.29 FQGGTSLTRR153 pKa = 11.84 AQISCYY159 pKa = 9.55 TPTVSHH165 pKa = 6.85 ALWQDD170 pKa = 3.29 AQRR173 pKa = 11.84 TPMVLPPARR182 pKa = 11.84 LKK184 pKa = 10.46 GRR186 pKa = 11.84 NWYY189 pKa = 8.44 TPDD192 pKa = 4.39 SGDD195 pKa = 5.27 DD196 pKa = 3.42 VDD198 pKa = 4.93 KK199 pKa = 11.49 GPGRR203 pKa = 11.84 ILIAADD209 pKa = 3.95 GQSSTVGSVPLGEE222 pKa = 3.87 IWACYY227 pKa = 8.82 KK228 pKa = 10.85 VKK230 pKa = 10.67 FSGTRR235 pKa = 11.84 SAXQLQSVQLNVQHH249 pKa = 6.64 QMTTAYY255 pKa = 8.52 HH256 pKa = 5.2 TVSLRR261 pKa = 11.84 AVPRR265 pKa = 11.84 GPLYY269 pKa = 10.26 IWTKK273 pKa = 10.84 SDD275 pKa = 3.7 PAPLNVWTSVEE286 pKa = 4.13 EE287 pKa = 4.14 ASTTGYY293 pKa = 10.46 HH294 pKa = 5.43 VATVGSPNSLLLEE307 pKa = 4.21 PMTIRR312 pKa = 11.84 DD313 pKa = 3.61 SSKK316 pKa = 9.71 QGAFSFEE323 pKa = 4.0 RR324 pKa = 11.84 ARR326 pKa = 11.84 VDD328 pKa = 3.57 DD329 pKa = 5.92 AIFQISTYY337 pKa = 9.19 SLPYY341 pKa = 8.52 GAYY344 pKa = 9.22 MLTLDD349 pKa = 5.24 VEE351 pKa = 4.32 LTPWGSEE358 pKa = 4.45 VMPTWDD364 pKa = 4.36 FSKK367 pKa = 10.07 PWTVEE372 pKa = 3.77 LVPAAGMKK380 pKa = 10.0 ILTDD384 pKa = 4.33 WIIHH388 pKa = 5.58 RR389 pKa = 11.84 LQWNANSPQVRR400 pKa = 11.84 LIARR404 pKa = 11.84 AMISVTSAYY413 pKa = 10.19 LPRR416 pKa = 11.84 VEE418 pKa = 4.94 FGCRR422 pKa = 11.84 AAVDD426 pKa = 4.06 SPADD430 pKa = 3.47 VIFGFRR436 pKa = 11.84 FEE438 pKa = 5.47 AYY440 pKa = 7.34 WATGYY445 pKa = 9.66 VRR447 pKa = 11.84 RR448 pKa = 11.84 PRR450 pKa = 11.84 YY451 pKa = 9.29 HH452 pKa = 7.14 SSEE455 pKa = 4.06 AVLVQADD462 pKa = 4.02 EE463 pKa = 4.7 GAPSLDD469 pKa = 3.3 EE470 pKa = 6.22 DD471 pKa = 3.43 EE472 pKa = 4.44 WQLVKK477 pKa = 11.06 AA478 pKa = 4.72
Molecular weight: 53.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.781
IPC2_protein 8.785
IPC_protein 8.756
Toseland 9.238
ProMoST 9.136
Dawson 9.575
Bjellqvist 9.355
Wikipedia 9.794
Rodwell 9.721
Grimsley 9.677
Solomon 9.619
Lehninger 9.575
Nozaki 9.341
DTASelect 9.326
Thurlkill 9.399
EMBOSS 9.692
Sillero 9.531
Patrickios 4.749
IPC_peptide 9.604
IPC2_peptide 8.024
IPC2.peptide.svr19 7.767
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
1329
265
586
443.0
50.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.299 ± 0.769
0.903 ± 0.359
5.719 ± 0.372
5.794 ± 0.667
3.988 ± 0.44
6.622 ± 0.25
1.655 ± 0.209
4.214 ± 0.62
4.891 ± 0.292
9.18 ± 1.348
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.784 ± 0.39
2.257 ± 0.337
5.267 ± 0.335
4.364 ± 0.187
7.148 ± 0.113
7.6 ± 0.513
6.02 ± 0.764
7.374 ± 0.58
3.236 ± 0.581
3.612 ± 0.148
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here