Amino acid dipepetide frequency for Flavobacterium phage Fpv6

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.66AlaAla: 2.66 ± 0.501
0.591AlaCys: 0.591 ± 0.203
1.921AlaAsp: 1.921 ± 0.344
2.882AlaGlu: 2.882 ± 0.713
2.808AlaPhe: 2.808 ± 0.372
3.473AlaGly: 3.473 ± 0.723
0.296AlaHis: 0.296 ± 0.132
4.507AlaIle: 4.507 ± 0.449
4.433AlaLys: 4.433 ± 0.642
3.621AlaLeu: 3.621 ± 0.868
0.961AlaMet: 0.961 ± 0.247
3.103AlaAsn: 3.103 ± 0.407
1.921AlaPro: 1.921 ± 0.351
2.217AlaGln: 2.217 ± 0.616
2.143AlaArg: 2.143 ± 0.309
2.956AlaSer: 2.956 ± 0.417
2.364AlaThr: 2.364 ± 0.378
1.699AlaVal: 1.699 ± 0.372
0.369AlaTrp: 0.369 ± 0.156
1.33AlaTyr: 1.33 ± 0.282
0.0AlaXaa: 0.0 ± 0.0
Cys
0.222CysAla: 0.222 ± 0.118
0.296CysCys: 0.296 ± 0.204
1.034CysAsp: 1.034 ± 0.265
1.256CysGlu: 1.256 ± 0.4
0.739CysPhe: 0.739 ± 0.265
0.813CysGly: 0.813 ± 0.307
0.074CysHis: 0.074 ± 0.071
0.887CysIle: 0.887 ± 0.24
0.961CysLys: 0.961 ± 0.316
1.034CysLeu: 1.034 ± 0.303
0.074CysMet: 0.074 ± 0.082
1.33CysAsn: 1.33 ± 0.399
0.517CysPro: 0.517 ± 0.2
0.739CysGln: 0.739 ± 0.245
0.443CysArg: 0.443 ± 0.174
0.591CysSer: 0.591 ± 0.213
0.222CysThr: 0.222 ± 0.124
0.369CysVal: 0.369 ± 0.157
0.074CysTrp: 0.074 ± 0.069
0.443CysTyr: 0.443 ± 0.16
0.0CysXaa: 0.0 ± 0.0
Asp
3.547AspAla: 3.547 ± 0.42
0.813AspCys: 0.813 ± 0.272
4.286AspAsp: 4.286 ± 0.608
4.581AspGlu: 4.581 ± 0.48
3.842AspPhe: 3.842 ± 0.53
3.842AspGly: 3.842 ± 0.602
0.222AspHis: 0.222 ± 0.121
3.842AspIle: 3.842 ± 0.456
4.655AspLys: 4.655 ± 0.555
5.911AspLeu: 5.911 ± 0.75
0.887AspMet: 0.887 ± 0.29
3.177AspAsn: 3.177 ± 0.415
1.699AspPro: 1.699 ± 0.351
1.108AspGln: 1.108 ± 0.226
1.552AspArg: 1.552 ± 0.281
2.956AspSer: 2.956 ± 0.488
2.882AspThr: 2.882 ± 0.488
4.138AspVal: 4.138 ± 0.629
0.813AspTrp: 0.813 ± 0.247
3.325AspTyr: 3.325 ± 0.462
0.0AspXaa: 0.0 ± 0.0
Glu
3.029GluAla: 3.029 ± 0.7
1.256GluCys: 1.256 ± 0.369
2.438GluAsp: 2.438 ± 0.449
4.581GluGlu: 4.581 ± 0.802
3.473GluPhe: 3.473 ± 0.508
1.773GluGly: 1.773 ± 0.336
0.961GluHis: 0.961 ± 0.274
10.049GluIle: 10.049 ± 1.072
9.014GluLys: 9.014 ± 0.969
9.162GluLeu: 9.162 ± 0.802
1.552GluMet: 1.552 ± 0.333
7.167GluAsn: 7.167 ± 0.769
2.586GluPro: 2.586 ± 0.528
2.882GluGln: 2.882 ± 0.569
2.217GluArg: 2.217 ± 0.56
4.064GluSer: 4.064 ± 0.434
5.394GluThr: 5.394 ± 0.8
3.251GluVal: 3.251 ± 0.531
1.108GluTrp: 1.108 ± 0.307
2.291GluTyr: 2.291 ± 0.376
0.0GluXaa: 0.0 ± 0.0
Phe
2.66PheAla: 2.66 ± 0.4
0.296PheCys: 0.296 ± 0.146
4.581PheAsp: 4.581 ± 0.572
5.468PheGlu: 5.468 ± 0.621
3.399PhePhe: 3.399 ± 0.514
2.734PheGly: 2.734 ± 0.477
1.108PheHis: 1.108 ± 0.328
4.138PheIle: 4.138 ± 0.678
5.246PheLys: 5.246 ± 0.694
3.399PheLeu: 3.399 ± 0.443
1.256PheMet: 1.256 ± 0.315
3.916PheAsn: 3.916 ± 0.677
1.478PhePro: 1.478 ± 0.38
2.882PheGln: 2.882 ± 0.546
1.921PheArg: 1.921 ± 0.423
2.808PheSer: 2.808 ± 0.429
3.251PheThr: 3.251 ± 0.468
3.621PheVal: 3.621 ± 0.511
0.517PheTrp: 0.517 ± 0.145
2.291PheTyr: 2.291 ± 0.465
0.0PheXaa: 0.0 ± 0.0
Gly
1.773GlyAla: 1.773 ± 0.427
0.739GlyCys: 0.739 ± 0.258
2.512GlyAsp: 2.512 ± 0.486
3.029GlyGlu: 3.029 ± 0.483
3.251GlyPhe: 3.251 ± 0.545
3.399GlyGly: 3.399 ± 0.598
0.443GlyHis: 0.443 ± 0.182
3.99GlyIle: 3.99 ± 0.522
3.99GlyLys: 3.99 ± 0.632
2.956GlyLeu: 2.956 ± 0.517
1.478GlyMet: 1.478 ± 0.299
3.029GlyAsn: 3.029 ± 0.48
0.517GlyPro: 0.517 ± 0.199
2.882GlyGln: 2.882 ± 0.454
2.069GlyArg: 2.069 ± 0.467
3.399GlySer: 3.399 ± 0.567
3.251GlyThr: 3.251 ± 0.5
3.547GlyVal: 3.547 ± 0.643
0.813GlyTrp: 0.813 ± 0.274
1.921GlyTyr: 1.921 ± 0.361
0.0GlyXaa: 0.0 ± 0.0
His
0.222HisAla: 0.222 ± 0.132
0.222HisCys: 0.222 ± 0.125
0.369HisAsp: 0.369 ± 0.161
1.108HisGlu: 1.108 ± 0.312
0.961HisPhe: 0.961 ± 0.306
0.665HisGly: 0.665 ± 0.176
0.296HisHis: 0.296 ± 0.147
1.108HisIle: 1.108 ± 0.289
1.256HisLys: 1.256 ± 0.4
1.256HisLeu: 1.256 ± 0.297
0.0HisMet: 0.0 ± 0.0
1.034HisAsn: 1.034 ± 0.251
0.665HisPro: 0.665 ± 0.211
0.296HisGln: 0.296 ± 0.133
0.369HisArg: 0.369 ± 0.143
1.256HisSer: 1.256 ± 0.303
0.665HisThr: 0.665 ± 0.239
0.443HisVal: 0.443 ± 0.226
0.222HisTrp: 0.222 ± 0.119
0.813HisTyr: 0.813 ± 0.188
0.0HisXaa: 0.0 ± 0.0
Ile
5.246IleAla: 5.246 ± 0.645
1.182IleCys: 1.182 ± 0.354
6.428IleAsp: 6.428 ± 0.671
8.645IleGlu: 8.645 ± 0.89
4.359IlePhe: 4.359 ± 0.481
4.212IleGly: 4.212 ± 0.404
1.404IleHis: 1.404 ± 0.384
6.502IleIle: 6.502 ± 0.842
7.832IleLys: 7.832 ± 0.851
5.468IleLeu: 5.468 ± 0.605
1.182IleMet: 1.182 ± 0.243
6.945IleAsn: 6.945 ± 0.674
2.364IlePro: 2.364 ± 0.376
3.547IleGln: 3.547 ± 0.48
2.66IleArg: 2.66 ± 0.493
5.246IleSer: 5.246 ± 0.568
5.024IleThr: 5.024 ± 0.552
4.507IleVal: 4.507 ± 0.691
0.591IleTrp: 0.591 ± 0.249
2.808IleTyr: 2.808 ± 0.498
0.0IleXaa: 0.0 ± 0.0
Lys
3.916LysAla: 3.916 ± 0.763
0.887LysCys: 0.887 ± 0.25
4.433LysAsp: 4.433 ± 0.756
9.384LysGlu: 9.384 ± 1.346
5.098LysPhe: 5.098 ± 0.658
3.473LysGly: 3.473 ± 0.461
1.33LysHis: 1.33 ± 0.237
9.236LysIle: 9.236 ± 0.884
11.009LysLys: 11.009 ± 1.211
7.832LysLeu: 7.832 ± 0.605
3.99LysMet: 3.99 ± 0.609
6.724LysAsn: 6.724 ± 0.712
2.882LysPro: 2.882 ± 0.554
4.803LysGln: 4.803 ± 0.898
4.507LysArg: 4.507 ± 0.632
4.803LysSer: 4.803 ± 0.647
6.207LysThr: 6.207 ± 0.936
3.251LysVal: 3.251 ± 0.417
1.404LysTrp: 1.404 ± 0.337
4.286LysTyr: 4.286 ± 0.548
0.0LysXaa: 0.0 ± 0.0
Leu
3.694LeuAla: 3.694 ± 0.649
0.517LeuCys: 0.517 ± 0.205
4.286LeuAsp: 4.286 ± 0.517
6.133LeuGlu: 6.133 ± 0.802
4.803LeuPhe: 4.803 ± 0.578
3.694LeuGly: 3.694 ± 0.556
0.665LeuHis: 0.665 ± 0.194
7.167LeuIle: 7.167 ± 0.674
9.532LeuLys: 9.532 ± 0.797
6.502LeuLeu: 6.502 ± 0.72
1.773LeuMet: 1.773 ± 0.458
7.093LeuAsn: 7.093 ± 0.743
2.069LeuPro: 2.069 ± 0.439
3.621LeuGln: 3.621 ± 0.549
2.66LeuArg: 2.66 ± 0.55
4.581LeuSer: 4.581 ± 0.731
6.354LeuThr: 6.354 ± 0.667
2.956LeuVal: 2.956 ± 0.37
0.443LeuTrp: 0.443 ± 0.163
1.921LeuTyr: 1.921 ± 0.368
0.0LeuXaa: 0.0 ± 0.0
Met
1.626MetAla: 1.626 ± 0.403
0.443MetCys: 0.443 ± 0.173
0.739MetAsp: 0.739 ± 0.246
1.478MetGlu: 1.478 ± 0.339
1.404MetPhe: 1.404 ± 0.244
0.517MetGly: 0.517 ± 0.178
0.0MetHis: 0.0 ± 0.0
1.921MetIle: 1.921 ± 0.383
2.956MetLys: 2.956 ± 0.468
0.961MetLeu: 0.961 ± 0.266
0.887MetMet: 0.887 ± 0.284
2.143MetAsn: 2.143 ± 0.406
0.739MetPro: 0.739 ± 0.214
0.961MetGln: 0.961 ± 0.314
0.591MetArg: 0.591 ± 0.19
1.552MetSer: 1.552 ± 0.328
2.069MetThr: 2.069 ± 0.505
1.552MetVal: 1.552 ± 0.281
0.0MetTrp: 0.0 ± 0.0
0.148MetTyr: 0.148 ± 0.105
0.0MetXaa: 0.0 ± 0.0
Asn
3.99AsnAla: 3.99 ± 0.516
0.739AsnCys: 0.739 ± 0.241
5.172AsnAsp: 5.172 ± 0.633
6.28AsnGlu: 6.28 ± 0.66
4.507AsnPhe: 4.507 ± 0.753
5.542AsnGly: 5.542 ± 0.657
1.034AsnHis: 1.034 ± 0.244
5.024AsnIle: 5.024 ± 0.573
6.576AsnLys: 6.576 ± 0.726
5.468AsnLeu: 5.468 ± 0.568
1.034AsnMet: 1.034 ± 0.313
5.32AsnAsn: 5.32 ± 0.627
3.251AsnPro: 3.251 ± 0.448
4.359AsnGln: 4.359 ± 0.492
2.364AsnArg: 2.364 ± 0.416
3.694AsnSer: 3.694 ± 0.412
2.882AsnThr: 2.882 ± 0.606
4.433AsnVal: 4.433 ± 0.498
0.961AsnTrp: 0.961 ± 0.226
2.734AsnTyr: 2.734 ± 0.476
0.0AsnXaa: 0.0 ± 0.0
Pro
1.404ProAla: 1.404 ± 0.339
0.517ProCys: 0.517 ± 0.189
2.438ProAsp: 2.438 ± 0.369
2.512ProGlu: 2.512 ± 0.36
1.699ProPhe: 1.699 ± 0.39
1.33ProGly: 1.33 ± 0.288
0.443ProHis: 0.443 ± 0.155
2.66ProIle: 2.66 ± 0.435
2.66ProLys: 2.66 ± 0.495
1.847ProLeu: 1.847 ± 0.383
0.739ProMet: 0.739 ± 0.24
1.995ProAsn: 1.995 ± 0.487
0.517ProPro: 0.517 ± 0.196
1.552ProGln: 1.552 ± 0.357
0.591ProArg: 0.591 ± 0.191
1.552ProSer: 1.552 ± 0.343
2.291ProThr: 2.291 ± 0.435
1.626ProVal: 1.626 ± 0.29
0.0ProTrp: 0.0 ± 0.0
1.478ProTyr: 1.478 ± 0.311
0.0ProXaa: 0.0 ± 0.0
Gln
1.995GlnAla: 1.995 ± 0.337
0.517GlnCys: 0.517 ± 0.213
1.626GlnAsp: 1.626 ± 0.395
2.956GlnGlu: 2.956 ± 0.547
1.773GlnPhe: 1.773 ± 0.311
1.478GlnGly: 1.478 ± 0.384
0.739GlnHis: 0.739 ± 0.235
5.468GlnIle: 5.468 ± 0.886
4.581GlnLys: 4.581 ± 0.923
3.916GlnLeu: 3.916 ± 0.585
1.995GlnMet: 1.995 ± 0.387
3.768GlnAsn: 3.768 ± 0.537
1.256GlnPro: 1.256 ± 0.29
1.699GlnGln: 1.699 ± 0.363
1.847GlnArg: 1.847 ± 0.673
2.512GlnSer: 2.512 ± 0.458
2.66GlnThr: 2.66 ± 0.354
1.478GlnVal: 1.478 ± 0.35
0.517GlnTrp: 0.517 ± 0.168
1.847GlnTyr: 1.847 ± 0.363
0.0GlnXaa: 0.0 ± 0.0
Arg
0.961ArgAla: 0.961 ± 0.286
0.591ArgCys: 0.591 ± 0.255
1.847ArgAsp: 1.847 ± 0.387
2.217ArgGlu: 2.217 ± 0.445
2.143ArgPhe: 2.143 ± 0.389
1.108ArgGly: 1.108 ± 0.255
0.369ArgHis: 0.369 ± 0.141
3.547ArgIle: 3.547 ± 0.382
3.399ArgLys: 3.399 ± 0.686
3.325ArgLeu: 3.325 ± 0.48
0.443ArgMet: 0.443 ± 0.166
2.364ArgAsn: 2.364 ± 0.404
0.665ArgPro: 0.665 ± 0.25
1.626ArgGln: 1.626 ± 0.513
0.887ArgArg: 0.887 ± 0.251
1.108ArgSer: 1.108 ± 0.29
1.847ArgThr: 1.847 ± 0.362
1.921ArgVal: 1.921 ± 0.404
0.296ArgTrp: 0.296 ± 0.138
1.921ArgTyr: 1.921 ± 0.353
0.0ArgXaa: 0.0 ± 0.0
Ser
2.143SerAla: 2.143 ± 0.469
0.665SerCys: 0.665 ± 0.218
3.547SerAsp: 3.547 ± 0.54
4.064SerGlu: 4.064 ± 0.623
3.473SerPhe: 3.473 ± 0.591
2.364SerGly: 2.364 ± 0.297
1.108SerHis: 1.108 ± 0.284
5.689SerIle: 5.689 ± 0.654
5.689SerLys: 5.689 ± 0.744
4.212SerLeu: 4.212 ± 0.596
1.182SerMet: 1.182 ± 0.291
4.286SerAsn: 4.286 ± 0.521
0.665SerPro: 0.665 ± 0.192
2.217SerGln: 2.217 ± 0.367
1.33SerArg: 1.33 ± 0.266
3.399SerSer: 3.399 ± 0.516
2.882SerThr: 2.882 ± 0.479
3.029SerVal: 3.029 ± 0.429
0.813SerTrp: 0.813 ± 0.251
1.699SerTyr: 1.699 ± 0.379
0.0SerXaa: 0.0 ± 0.0
Thr
2.364ThrAla: 2.364 ± 0.29
0.296ThrCys: 0.296 ± 0.134
2.956ThrAsp: 2.956 ± 0.369
3.99ThrGlu: 3.99 ± 0.499
3.621ThrPhe: 3.621 ± 0.476
3.103ThrGly: 3.103 ± 0.548
0.887ThrHis: 0.887 ± 0.338
4.803ThrIle: 4.803 ± 0.548
5.911ThrLys: 5.911 ± 0.717
4.803ThrLeu: 4.803 ± 0.56
1.33ThrMet: 1.33 ± 0.35
3.99ThrAsn: 3.99 ± 0.668
2.808ThrPro: 2.808 ± 0.543
2.364ThrGln: 2.364 ± 0.475
1.773ThrArg: 1.773 ± 0.338
3.029ThrSer: 3.029 ± 0.421
4.729ThrThr: 4.729 ± 0.672
3.547ThrVal: 3.547 ± 0.619
0.296ThrTrp: 0.296 ± 0.162
2.512ThrTyr: 2.512 ± 0.498
0.0ThrXaa: 0.0 ± 0.0
Val
3.029ValAla: 3.029 ± 0.499
0.591ValCys: 0.591 ± 0.228
4.212ValAsp: 4.212 ± 0.528
4.877ValGlu: 4.877 ± 0.689
2.217ValPhe: 2.217 ± 0.494
3.251ValGly: 3.251 ± 0.516
0.739ValHis: 0.739 ± 0.211
3.473ValIle: 3.473 ± 0.456
4.359ValLys: 4.359 ± 0.488
4.138ValLeu: 4.138 ± 0.548
1.182ValMet: 1.182 ± 0.264
3.547ValAsn: 3.547 ± 0.502
1.921ValPro: 1.921 ± 0.427
1.626ValGln: 1.626 ± 0.557
1.404ValArg: 1.404 ± 0.262
2.66ValSer: 2.66 ± 0.381
2.069ValThr: 2.069 ± 0.389
4.138ValVal: 4.138 ± 0.651
1.034ValTrp: 1.034 ± 0.276
2.512ValTyr: 2.512 ± 0.366
0.0ValXaa: 0.0 ± 0.0
Trp
0.443TrpAla: 0.443 ± 0.196
0.148TrpCys: 0.148 ± 0.081
0.665TrpAsp: 0.665 ± 0.197
0.517TrpGlu: 0.517 ± 0.225
0.591TrpPhe: 0.591 ± 0.212
0.517TrpGly: 0.517 ± 0.161
0.074TrpHis: 0.074 ± 0.066
0.739TrpIle: 0.739 ± 0.264
1.182TrpLys: 1.182 ± 0.301
1.33TrpLeu: 1.33 ± 0.361
0.148TrpMet: 0.148 ± 0.104
1.108TrpAsn: 1.108 ± 0.386
0.074TrpPro: 0.074 ± 0.083
0.739TrpGln: 0.739 ± 0.234
0.369TrpArg: 0.369 ± 0.14
0.739TrpSer: 0.739 ± 0.203
0.369TrpThr: 0.369 ± 0.153
0.961TrpVal: 0.961 ± 0.225
0.222TrpTrp: 0.222 ± 0.134
0.0TrpTyr: 0.0 ± 0.0
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.33TyrAla: 1.33 ± 0.228
0.813TyrCys: 0.813 ± 0.254
2.586TyrAsp: 2.586 ± 0.372
2.291TyrGlu: 2.291 ± 0.488
2.808TyrPhe: 2.808 ± 0.452
1.699TyrGly: 1.699 ± 0.31
1.034TyrHis: 1.034 ± 0.348
1.404TyrIle: 1.404 ± 0.339
4.212TyrLys: 4.212 ± 0.553
3.399TyrLeu: 3.399 ± 0.441
0.517TyrMet: 0.517 ± 0.197
3.325TyrAsn: 3.325 ± 0.504
1.256TyrPro: 1.256 ± 0.27
2.512TyrGln: 2.512 ± 0.486
0.887TyrArg: 0.887 ± 0.322
1.552TyrSer: 1.552 ± 0.377
1.699TyrThr: 1.699 ± 0.302
2.512TyrVal: 2.512 ± 0.393
0.443TyrTrp: 0.443 ± 0.19
1.182TyrTyr: 1.182 ± 0.341
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 58 proteins (13535 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski