Pandoravirus macleodensis
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 926 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2U7UE55|A0A2U7UE55_9VIRU Uncharacterized protein OS=Pandoravirus macleodensis OX=2107707 GN=pmac_cds_29 PE=4 SV=1
MM1 pKa = 7.74 SDD3 pKa = 3.08 PRR5 pKa = 11.84 LGYY8 pKa = 10.06 AYY10 pKa = 10.31 SYY12 pKa = 10.63 GAEE15 pKa = 3.66 PSGFVGAAPPSAAGAVWRR33 pKa = 11.84 GTGAPRR39 pKa = 11.84 TRR41 pKa = 11.84 EE42 pKa = 3.98 LGRR45 pKa = 11.84 SDD47 pKa = 4.11 AAVPLAWNAQQPFGAGLAAQQHH69 pKa = 5.88 TLRR72 pKa = 11.84 QPPPGYY78 pKa = 9.53 GRR80 pKa = 11.84 SYY82 pKa = 10.87 GGTGVAPYY90 pKa = 10.32 ASSGYY95 pKa = 10.56 SDD97 pKa = 5.4 DD98 pKa = 4.24 EE99 pKa = 4.74 GSAGSDD105 pKa = 2.94 ADD107 pKa = 3.73 EE108 pKa = 4.93 AFSDD112 pKa = 4.26 EE113 pKa = 5.01 AGSDD117 pKa = 3.6 DD118 pKa = 4.82 DD119 pKa = 5.91 DD120 pKa = 4.28 GVVYY124 pKa = 10.8 SDD126 pKa = 4.97 DD127 pKa = 3.87 EE128 pKa = 5.04 NGDD131 pKa = 3.41 GHH133 pKa = 6.41 EE134 pKa = 4.4 SARR137 pKa = 11.84 GSWGGAVYY145 pKa = 10.01 GAAPPQSSWWW155 pKa = 3.52
Molecular weight: 15.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.804
IPC2_protein 4.037
IPC_protein 3.999
Toseland 3.795
ProMoST 4.177
Dawson 3.999
Bjellqvist 4.151
Wikipedia 3.948
Rodwell 3.834
Grimsley 3.706
Solomon 3.986
Lehninger 3.948
Nozaki 4.113
DTASelect 4.368
Thurlkill 3.846
EMBOSS 3.948
Sillero 4.126
Patrickios 1.952
IPC_peptide 3.986
IPC2_peptide 4.101
IPC2.peptide.svr19 4.01
Protein with the highest isoelectric point:
>tr|A0A2U7UFF9|A0A2U7UFF9_9VIRU Uncharacterized protein OS=Pandoravirus macleodensis OX=2107707 GN=pmac_cds_530 PE=4 SV=1
MM1 pKa = 7.01 QGKK4 pKa = 9.65 SKK6 pKa = 10.83 GAGAKK11 pKa = 7.93 GTTRR15 pKa = 11.84 ANGQRR20 pKa = 11.84 QNALGRR26 pKa = 11.84 PRR28 pKa = 11.84 GAQPHH33 pKa = 5.92 SGVPRR38 pKa = 11.84 TYY40 pKa = 10.53 LVPIRR45 pKa = 11.84 KK46 pKa = 8.81 RR47 pKa = 11.84 VATPAAVLASLGVYY61 pKa = 10.42 GNANARR67 pKa = 11.84 GAGHH71 pKa = 7.38 AEE73 pKa = 4.08 QEE75 pKa = 4.27 NEE77 pKa = 4.48 HH78 pKa = 6.51 YY79 pKa = 10.83 DD80 pKa = 3.66 ADD82 pKa = 4.0 SDD84 pKa = 3.8 ATTTVVTFVRR94 pKa = 11.84 LTKK97 pKa = 10.67 AQMARR102 pKa = 11.84 GRR104 pKa = 11.84 RR105 pKa = 11.84 RR106 pKa = 11.84 AWEE109 pKa = 3.86 EE110 pKa = 3.35 RR111 pKa = 11.84 CARR114 pKa = 11.84 RR115 pKa = 11.84 RR116 pKa = 11.84 ARR118 pKa = 11.84 SQQPVPASQACGQCNATVYY137 pKa = 10.4 GAVDD141 pKa = 3.52 MCPSCGAALVHH152 pKa = 6.58 IVAAVAADD160 pKa = 3.54 QDD162 pKa = 3.48 VDD164 pKa = 3.53 RR165 pKa = 6.46
Molecular weight: 17.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.353
IPC2_protein 9.487
IPC_protein 10.277
Toseland 10.35
ProMoST 10.321
Dawson 10.511
Bjellqvist 10.277
Wikipedia 10.745
Rodwell 10.628
Grimsley 10.584
Solomon 10.613
Lehninger 10.57
Nozaki 10.423
DTASelect 10.248
Thurlkill 10.394
EMBOSS 10.76
Sillero 10.452
Patrickios 10.335
IPC_peptide 10.613
IPC2_peptide 9.619
IPC2.peptide.svr19 8.547
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
926
0
926
374449
49
2341
404.4
43.63
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.695 ± 0.095
2.481 ± 0.056
7.628 ± 0.088
3.926 ± 0.06
2.277 ± 0.036
7.797 ± 0.095
2.931 ± 0.051
3.369 ± 0.036
2.044 ± 0.056
7.93 ± 0.072
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.113 ± 0.034
2.477 ± 0.034
6.25 ± 0.073
2.811 ± 0.056
8.337 ± 0.074
6.343 ± 0.077
5.91 ± 0.063
7.488 ± 0.066
1.908 ± 0.042
2.285 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here