Streptococcus phage CHPC1152
Average proteome isoelectric point is 6.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 44 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3G8FD52|A0A3G8FD52_9CAUD Uncharacterized protein OS=Streptococcus phage CHPC1152 OX=2365029 GN=CHPC1152_0041 PE=4 SV=1
MM1 pKa = 7.56 SVPQSIVNWFVIHH14 pKa = 7.14 RR15 pKa = 11.84 NLLTYY20 pKa = 11.39 SMFGSRR26 pKa = 11.84 NGSDD30 pKa = 3.03 GTADD34 pKa = 3.56 CSGSMSQALKK44 pKa = 10.12 EE45 pKa = 4.04 AGIGIQGLPSTVTLGQQLAKK65 pKa = 10.58 NGFYY69 pKa = 10.18 RR70 pKa = 11.84 VSINQDD76 pKa = 2.14 WDD78 pKa = 3.55 ALTGDD83 pKa = 3.9 IVMMSWGADD92 pKa = 3.23 MSQSGGAGGHH102 pKa = 5.81 VGVMMDD108 pKa = 3.26 SVNFISCDD116 pKa = 3.3 YY117 pKa = 8.82 STQGAVGQAINTYY130 pKa = 8.91 PWNDD134 pKa = 3.08 YY135 pKa = 10.16 YY136 pKa = 11.52 AANKK140 pKa = 9.07 PNYY143 pKa = 9.09 IEE145 pKa = 3.75 VWRR148 pKa = 11.84 YY149 pKa = 10.19 AEE151 pKa = 5.22 SAPQTNNQANTAVVPQQKK169 pKa = 10.42 AYY171 pKa = 11.08 YY172 pKa = 8.08 EE173 pKa = 4.27 ANEE176 pKa = 3.96 VKK178 pKa = 10.25 YY179 pKa = 11.21 VNGIWQIKK187 pKa = 9.47 CDD189 pKa = 4.14 YY190 pKa = 9.46 LCPIGFDD197 pKa = 3.08 WTEE200 pKa = 3.67 NGIPVAMVNWVDD212 pKa = 4.34 KK213 pKa = 11.12 DD214 pKa = 4.24 GNDD217 pKa = 3.97 LPDD220 pKa = 4.26 GADD223 pKa = 3.29 QEE225 pKa = 5.13 FKK227 pKa = 11.28 AGMFFSFAGDD237 pKa = 3.56 EE238 pKa = 4.15 NNITDD243 pKa = 3.82 TGEE246 pKa = 3.65 GGYY249 pKa = 10.61 YY250 pKa = 9.76 GGYY253 pKa = 8.0 YY254 pKa = 9.41 YY255 pKa = 10.76 RR256 pKa = 11.84 RR257 pKa = 11.84 FEE259 pKa = 4.06 FGQFGTVWLSCWNKK273 pKa = 10.66 DD274 pKa = 3.89 DD275 pKa = 5.14 LVNYY279 pKa = 7.38 YY280 pKa = 8.26 QQ281 pKa = 4.22
Molecular weight: 31.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.077
IPC2_protein 4.19
IPC_protein 4.177
Toseland 3.961
ProMoST 4.329
Dawson 4.151
Bjellqvist 4.304
Wikipedia 4.088
Rodwell 3.999
Grimsley 3.872
Solomon 4.151
Lehninger 4.113
Nozaki 4.266
DTASelect 4.52
Thurlkill 4.012
EMBOSS 4.101
Sillero 4.291
Patrickios 0.35
IPC_peptide 4.151
IPC2_peptide 4.266
IPC2.peptide.svr19 4.168
Protein with the highest isoelectric point:
>tr|A0A3G8FD37|A0A3G8FD37_9CAUD Uncharacterized protein OS=Streptococcus phage CHPC1152 OX=2365029 GN=CHPC1152_0012 PE=4 SV=1
MM1 pKa = 7.83 ADD3 pKa = 3.75 FGVSLLEE10 pKa = 4.01 ARR12 pKa = 11.84 RR13 pKa = 11.84 MTLKK17 pKa = 10.49 EE18 pKa = 3.53 MKK20 pKa = 10.01 LYY22 pKa = 10.64 QKK24 pKa = 10.3 AHH26 pKa = 7.05 KK27 pKa = 10.13 KK28 pKa = 10.28 RR29 pKa = 11.84 FLNKK33 pKa = 9.47 EE34 pKa = 3.69 RR35 pKa = 11.84 EE36 pKa = 4.36 IYY38 pKa = 9.68 QLAYY42 pKa = 10.87 LNRR45 pKa = 11.84 LANATTKK52 pKa = 10.62 DD53 pKa = 3.03 GKK55 pKa = 10.64 KK56 pKa = 10.46 YY57 pKa = 10.49 YY58 pKa = 10.19 FEE60 pKa = 5.48 KK61 pKa = 10.78 FDD63 pKa = 4.29 DD64 pKa = 4.62 FYY66 pKa = 11.69 NSKK69 pKa = 9.89 EE70 pKa = 3.85 RR71 pKa = 11.84 ARR73 pKa = 11.84 EE74 pKa = 3.77 VLGEE78 pKa = 4.35 KK79 pKa = 8.61 ITNSKK84 pKa = 10.24 LLEE87 pKa = 4.07 RR88 pKa = 11.84 AKK90 pKa = 11.02 KK91 pKa = 9.09 NLNYY95 pKa = 10.73 KK96 pKa = 9.97 LDD98 pKa = 4.05 RR99 pKa = 11.84 GLLDD103 pKa = 3.42 GRR105 pKa = 4.51
Molecular weight: 12.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.075
IPC2_protein 9.341
IPC_protein 9.297
Toseland 10.072
ProMoST 9.765
Dawson 10.248
Bjellqvist 9.882
Wikipedia 10.394
Rodwell 10.774
Grimsley 10.306
Solomon 10.277
Lehninger 10.248
Nozaki 10.028
DTASelect 9.882
Thurlkill 10.101
EMBOSS 10.467
Sillero 10.145
Patrickios 10.438
IPC_peptide 10.277
IPC2_peptide 8.317
IPC2.peptide.svr19 8.348
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
44
0
44
10537
40
1545
239.5
26.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.849 ± 1.273
0.512 ± 0.121
6.093 ± 0.528
6.966 ± 0.866
4.052 ± 0.178
6.843 ± 0.568
1.367 ± 0.218
7.013 ± 0.387
8.095 ± 0.696
7.773 ± 0.323
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.496 ± 0.314
5.732 ± 0.364
2.838 ± 0.25
3.787 ± 0.258
4.28 ± 0.467
6.71 ± 0.696
6.045 ± 0.309
6.729 ± 0.276
1.101 ± 0.181
3.72 ± 0.472
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here