Amino acid dipepetide frequency for Phaeodactylum tricornutum (strain CCAP 1055/1)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.616AlaAla: 8.616 ± 0.061	1.351AlaCys: 1.351 ± 0.019	4.267AlaAsp: 4.267 ± 0.033	4.597AlaGlu: 4.597 ± 0.039	3.385AlaPhe: 3.385 ± 0.026	4.976AlaGly: 4.976 ± 0.036	1.825AlaHis: 1.825 ± 0.019	4.121AlaIle: 4.121 ± 0.028	3.977AlaLys: 3.977 ± 0.031	8.646AlaLeu: 8.646 ± 0.055	2.149AlaMet: 2.149 ± 0.021	3.022AlaAsn: 3.022 ± 0.029	4.032AlaPro: 4.032 ± 0.045	3.1AlaGln: 3.1 ± 0.028	4.616AlaArg: 4.616 ± 0.036	7.338AlaSer: 7.338 ± 0.046	5.751AlaThr: 5.751 ± 0.041	6.171AlaVal: 6.171 ± 0.042	1.119AlaTrp: 1.119 ± 0.017	2.061AlaTyr: 2.061 ± 0.023	0.0AlaXaa: 0.0 ± 0.0
Cys	1.147CysAla: 1.147 ± 0.017	0.409CysCys: 0.409 ± 0.012	0.852CysAsp: 0.852 ± 0.015	0.812CysGlu: 0.812 ± 0.015	0.688CysPhe: 0.688 ± 0.012	1.115CysGly: 1.115 ± 0.015	0.413CysHis: 0.413 ± 0.01	0.769CysIle: 0.769 ± 0.014	0.575CysLys: 0.575 ± 0.01	1.618CysLeu: 1.618 ± 0.02	0.343CysMet: 0.343 ± 0.009	0.543CysAsn: 0.543 ± 0.011	0.785CysPro: 0.785 ± 0.016	0.649CysGln: 0.649 ± 0.012	1.061CysArg: 1.061 ± 0.015	1.199CysSer: 1.199 ± 0.017	0.874CysThr: 0.874 ± 0.013	1.159CysVal: 1.159 ± 0.017	0.253CysTrp: 0.253 ± 0.007	0.432CysTyr: 0.432 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	4.992AspAla: 4.992 ± 0.039	0.899AspCys: 0.899 ± 0.017	4.751AspAsp: 4.751 ± 0.05	4.592AspGlu: 4.592 ± 0.039	2.384AspPhe: 2.384 ± 0.022	4.257AspGly: 4.257 ± 0.039	1.269AspHis: 1.269 ± 0.017	2.638AspIle: 2.638 ± 0.029	2.309AspLys: 2.309 ± 0.023	5.131AspLeu: 5.131 ± 0.036	1.251AspMet: 1.251 ± 0.018	2.042AspAsn: 2.042 ± 0.021	3.121AspPro: 3.121 ± 0.025	1.983AspGln: 1.983 ± 0.019	3.212AspArg: 3.212 ± 0.025	4.415AspSer: 4.415 ± 0.035	3.269AspThr: 3.269 ± 0.027	4.058AspVal: 4.058 ± 0.029	0.857AspTrp: 0.857 ± 0.014	1.499AspTyr: 1.499 ± 0.021	0.0AspXaa: 0.0 ± 0.0
Glu	5.244GluAla: 5.244 ± 0.038	0.836GluCys: 0.836 ± 0.014	3.986GluAsp: 3.986 ± 0.035	5.009GluGlu: 5.009 ± 0.048	1.931GluPhe: 1.931 ± 0.02	3.43GluGly: 3.43 ± 0.04	1.431GluHis: 1.431 ± 0.019	3.026GluIle: 3.026 ± 0.027	3.359GluLys: 3.359 ± 0.034	5.323GluLeu: 5.323 ± 0.035	1.479GluMet: 1.479 ± 0.018	2.501GluAsn: 2.501 ± 0.024	2.358GluPro: 2.358 ± 0.026	2.55GluGln: 2.55 ± 0.027	3.918GluArg: 3.918 ± 0.032	4.858GluSer: 4.858 ± 0.036	3.828GluThr: 3.828 ± 0.029	3.339GluVal: 3.339 ± 0.026	0.839GluTrp: 0.839 ± 0.012	1.58GluTyr: 1.58 ± 0.019	0.0GluXaa: 0.0 ± 0.0
Phe	3.191PheAla: 3.191 ± 0.028	0.804PheCys: 0.804 ± 0.014	2.404PheAsp: 2.404 ± 0.027	2.088PheGlu: 2.088 ± 0.019	1.68PhePhe: 1.68 ± 0.019	2.893PheGly: 2.893 ± 0.031	0.983PheHis: 0.983 ± 0.015	1.351PheIle: 1.351 ± 0.017	1.146PheLys: 1.146 ± 0.016	3.945PheLeu: 3.945 ± 0.033	0.719PheMet: 0.719 ± 0.013	1.141PheAsn: 1.141 ± 0.016	1.918PhePro: 1.918 ± 0.017	1.511PheGln: 1.511 ± 0.016	2.251PheArg: 2.251 ± 0.021	2.948PheSer: 2.948 ± 0.024	1.875PheThr: 1.875 ± 0.022	2.931PheVal: 2.931 ± 0.026	0.605PheTrp: 0.605 ± 0.013	1.086PheTyr: 1.086 ± 0.016	0.0PheXaa: 0.0 ± 0.0
Gly	4.854GlyAla: 4.854 ± 0.04	1.086GlyCys: 1.086 ± 0.022	3.617GlyAsp: 3.617 ± 0.027	3.342GlyGlu: 3.342 ± 0.032	2.562GlyPhe: 2.562 ± 0.027	5.072GlyGly: 5.072 ± 0.053	1.465GlyHis: 1.465 ± 0.018	3.366GlyIle: 3.366 ± 0.033	3.39GlyLys: 3.39 ± 0.033	5.549GlyLeu: 5.549 ± 0.039	1.636GlyMet: 1.636 ± 0.02	2.694GlyAsn: 2.694 ± 0.027	2.622GlyPro: 2.622 ± 0.028	2.174GlyGln: 2.174 ± 0.022	3.857GlyArg: 3.857 ± 0.031	5.492GlySer: 5.492 ± 0.044	4.153GlyThr: 4.153 ± 0.04	4.294GlyVal: 4.294 ± 0.038	0.96GlyTrp: 0.96 ± 0.017	1.753GlyTyr: 1.753 ± 0.024	0.0GlyXaa: 0.0 ± 0.0
His	1.984HisAla: 1.984 ± 0.022	0.447HisCys: 0.447 ± 0.011	1.422HisAsp: 1.422 ± 0.018	1.404HisGlu: 1.404 ± 0.02	0.98HisPhe: 0.98 ± 0.013	1.749HisGly: 1.749 ± 0.021	0.844HisHis: 0.844 ± 0.016	1.007HisIle: 1.007 ± 0.014	0.95HisLys: 0.95 ± 0.015	2.164HisLeu: 2.164 ± 0.021	0.477HisMet: 0.477 ± 0.01	0.89HisAsn: 0.89 ± 0.012	1.454HisPro: 1.454 ± 0.019	1.085HisGln: 1.085 ± 0.015	1.692HisArg: 1.692 ± 0.02	1.916HisSer: 1.916 ± 0.021	1.313HisThr: 1.313 ± 0.018	1.794HisVal: 1.794 ± 0.02	0.383HisTrp: 0.383 ± 0.008	0.664HisTyr: 0.664 ± 0.013	0.0HisXaa: 0.0 ± 0.0
Ile	4.02IleAla: 4.02 ± 0.034	0.757IleCys: 0.757 ± 0.014	2.853IleAsp: 2.853 ± 0.025	2.721IleGlu: 2.721 ± 0.027	1.744IlePhe: 1.744 ± 0.022	3.027IleGly: 3.027 ± 0.029	1.154IleHis: 1.154 ± 0.015	1.778IleIle: 1.778 ± 0.021	1.773IleLys: 1.773 ± 0.02	4.816IleLeu: 4.816 ± 0.037	0.937IleMet: 0.937 ± 0.015	1.451IleAsn: 1.451 ± 0.018	2.829IlePro: 2.829 ± 0.025	1.929IleGln: 1.929 ± 0.02	2.817IleArg: 2.817 ± 0.024	3.372IleSer: 3.372 ± 0.029	2.173IleThr: 2.173 ± 0.025	3.571IleVal: 3.571 ± 0.027	0.507IleTrp: 0.507 ± 0.012	1.069IleTyr: 1.069 ± 0.016	0.0IleXaa: 0.0 ± 0.0
Lys	4.138LysAla: 4.138 ± 0.033	0.568LysCys: 0.568 ± 0.012	2.67LysAsp: 2.67 ± 0.024	3.199LysGlu: 3.199 ± 0.03	1.378LysPhe: 1.378 ± 0.016	2.703LysGly: 2.703 ± 0.029	1.122LysHis: 1.122 ± 0.016	2.231LysIle: 2.231 ± 0.021	3.109LysLys: 3.109 ± 0.037	4.062LysLeu: 4.062 ± 0.032	1.103LysMet: 1.103 ± 0.015	1.841LysAsn: 1.841 ± 0.022	2.186LysPro: 2.186 ± 0.019	1.93LysGln: 1.93 ± 0.023	3.259LysArg: 3.259 ± 0.028	3.599LysSer: 3.599 ± 0.031	2.847LysThr: 2.847 ± 0.027	2.918LysVal: 2.918 ± 0.026	0.525LysTrp: 0.525 ± 0.012	1.204LysTyr: 1.204 ± 0.016	0.0LysXaa: 0.0 ± 0.0
Leu	8.281LeuAla: 8.281 ± 0.048	1.62LeuCys: 1.62 ± 0.02	5.408LeuAsp: 5.408 ± 0.033	5.957LeuGlu: 5.957 ± 0.042	3.466LeuPhe: 3.466 ± 0.032	5.709LeuGly: 5.709 ± 0.058	2.414LeuHis: 2.414 ± 0.025	3.572LeuIle: 3.572 ± 0.033	3.965LeuLys: 3.965 ± 0.033	9.795LeuLeu: 9.795 ± 0.067	1.915LeuMet: 1.915 ± 0.022	3.103LeuAsn: 3.103 ± 0.027	5.382LeuPro: 5.382 ± 0.038	4.728LeuGln: 4.728 ± 0.041	6.006LeuArg: 6.006 ± 0.038	7.848LeuSer: 7.848 ± 0.047	5.247LeuThr: 5.247 ± 0.037	6.398LeuVal: 6.398 ± 0.045	1.257LeuTrp: 1.257 ± 0.018	2.449LeuTyr: 2.449 ± 0.024	0.0LeuXaa: 0.0 ± 0.0
Met	2.06MetAla: 2.06 ± 0.021	0.286MetCys: 0.286 ± 0.008	1.442MetAsp: 1.442 ± 0.021	1.643MetGlu: 1.643 ± 0.02	0.701MetPhe: 0.701 ± 0.014	1.426MetGly: 1.426 ± 0.018	0.511MetHis: 0.511 ± 0.011	1.017MetIle: 1.017 ± 0.017	1.226MetLys: 1.226 ± 0.016	1.988MetLeu: 1.988 ± 0.021	0.621MetMet: 0.621 ± 0.012	0.883MetAsn: 0.883 ± 0.013	1.111MetPro: 1.111 ± 0.015	0.967MetGln: 0.967 ± 0.015	1.219MetArg: 1.219 ± 0.015	1.777MetSer: 1.777 ± 0.019	1.37MetThr: 1.37 ± 0.019	1.505MetVal: 1.505 ± 0.02	0.247MetTrp: 0.247 ± 0.008	0.53MetTyr: 0.53 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	3.283AsnAla: 3.283 ± 0.027	0.542AsnCys: 0.542 ± 0.011	2.417AsnAsp: 2.417 ± 0.023	2.422AsnGlu: 2.422 ± 0.024	1.365AsnPhe: 1.365 ± 0.019	2.756AsnGly: 2.756 ± 0.025	0.937AsnHis: 0.937 ± 0.015	1.733AsnIle: 1.733 ± 0.02	1.627AsnLys: 1.627 ± 0.02	3.378AsnLeu: 3.378 ± 0.029	0.831AsnMet: 0.831 ± 0.015	1.638AsnAsn: 1.638 ± 0.024	2.102AsnPro: 2.102 ± 0.023	1.386AsnGln: 1.386 ± 0.017	2.208AsnArg: 2.208 ± 0.021	2.891AsnSer: 2.891 ± 0.026	2.232AsnThr: 2.232 ± 0.022	2.738AsnVal: 2.738 ± 0.024	0.443AsnTrp: 0.443 ± 0.01	0.917AsnTyr: 0.917 ± 0.014	0.0AsnXaa: 0.0 ± 0.0
Pro	3.877ProAla: 3.877 ± 0.036	0.635ProCys: 0.635 ± 0.012	2.941ProAsp: 2.941 ± 0.025	3.014ProGlu: 3.014 ± 0.024	1.93ProPhe: 1.93 ± 0.023	2.823ProGly: 2.823 ± 0.022	1.347ProHis: 1.347 ± 0.017	2.337ProIle: 2.337 ± 0.021	2.238ProLys: 2.238 ± 0.025	4.657ProLeu: 4.657 ± 0.031	1.073ProMet: 1.073 ± 0.016	2.035ProAsn: 2.035 ± 0.019	3.751ProPro: 3.751 ± 0.052	1.88ProGln: 1.88 ± 0.023	2.758ProArg: 2.758 ± 0.026	5.288ProSer: 5.288 ± 0.052	3.874ProThr: 3.874 ± 0.042	3.501ProVal: 3.501 ± 0.031	0.658ProTrp: 0.658 ± 0.013	1.266ProTyr: 1.266 ± 0.018	0.0ProXaa: 0.0 ± 0.0
Gln	3.698GlnAla: 3.698 ± 0.032	0.546GlnCys: 0.546 ± 0.011	2.008GlnAsp: 2.008 ± 0.021	2.483GlnGlu: 2.483 ± 0.027	1.239GlnPhe: 1.239 ± 0.014	2.26GlnGly: 2.26 ± 0.022	1.196GlnHis: 1.196 ± 0.019	1.892GlnIle: 1.892 ± 0.02	2.074GlnLys: 2.074 ± 0.023	3.667GlnLeu: 3.667 ± 0.032	0.91GlnMet: 0.91 ± 0.016	1.604GlnAsn: 1.604 ± 0.018	1.99GlnPro: 1.99 ± 0.027	2.544GlnGln: 2.544 ± 0.045	2.988GlnArg: 2.988 ± 0.027	3.358GlnSer: 3.358 ± 0.028	2.369GlnThr: 2.369 ± 0.026	2.467GlnVal: 2.467 ± 0.026	0.545GlnTrp: 0.545 ± 0.011	1.077GlnTyr: 1.077 ± 0.017	0.0GlnXaa: 0.0 ± 0.0
Arg	4.511ArgAla: 4.511 ± 0.037	0.906ArgCys: 0.906 ± 0.016	3.395ArgAsp: 3.395 ± 0.031	3.48ArgGlu: 3.48 ± 0.028	2.259ArgPhe: 2.259 ± 0.023	3.436ArgGly: 3.436 ± 0.034	1.692ArgHis: 1.692 ± 0.022	3.035ArgIle: 3.035 ± 0.027	3.541ArgLys: 3.541 ± 0.029	5.75ArgLeu: 5.75 ± 0.037	1.434ArgMet: 1.434 ± 0.018	2.634ArgAsn: 2.634 ± 0.024	2.84ArgPro: 2.84 ± 0.025	2.892ArgGln: 2.892 ± 0.029	5.031ArgArg: 5.031 ± 0.042	4.893ArgSer: 4.893 ± 0.041	3.446ArgThr: 3.446 ± 0.027	3.798ArgVal: 3.798 ± 0.028	0.849ArgTrp: 0.849 ± 0.014	1.654ArgTyr: 1.654 ± 0.02	0.0ArgXaa: 0.0 ± 0.0
Ser	6.414SerAla: 6.414 ± 0.04	1.255SerCys: 1.255 ± 0.017	4.475SerAsp: 4.475 ± 0.039	4.238SerGlu: 4.238 ± 0.035	3.42SerPhe: 3.42 ± 0.028	5.224SerGly: 5.224 ± 0.043	1.889SerHis: 1.889 ± 0.024	3.983SerIle: 3.983 ± 0.034	3.877SerLys: 3.877 ± 0.034	7.944SerLeu: 7.944 ± 0.044	1.906SerMet: 1.906 ± 0.025	3.376SerAsn: 3.376 ± 0.03	4.437SerPro: 4.437 ± 0.046	3.07SerGln: 3.07 ± 0.032	4.955SerArg: 4.955 ± 0.04	8.918SerSer: 8.918 ± 0.07	5.724SerThr: 5.724 ± 0.043	5.571SerVal: 5.571 ± 0.035	1.111SerTrp: 1.111 ± 0.017	1.934SerTyr: 1.934 ± 0.023	0.0SerXaa: 0.0 ± 0.0
Thr	5.536ThrAla: 5.536 ± 0.043	0.907ThrCys: 0.907 ± 0.018	3.251ThrAsp: 3.251 ± 0.026	3.296ThrGlu: 3.296 ± 0.027	2.212ThrPhe: 2.212 ± 0.023	3.969ThrGly: 3.969 ± 0.038	1.364ThrHis: 1.364 ± 0.018	3.024ThrIle: 3.024 ± 0.027	2.845ThrLys: 2.845 ± 0.029	5.669ThrLeu: 5.669 ± 0.038	1.372ThrMet: 1.372 ± 0.017	2.458ThrAsn: 2.458 ± 0.023	3.569ThrPro: 3.569 ± 0.039	2.044ThrGln: 2.044 ± 0.019	3.224ThrArg: 3.224 ± 0.026	5.368ThrSer: 5.368 ± 0.039	4.563ThrThr: 4.563 ± 0.053	4.409ThrVal: 4.409 ± 0.033	0.75ThrTrp: 0.75 ± 0.014	1.42ThrTyr: 1.42 ± 0.019	0.0ThrXaa: 0.0 ± 0.0
Val	6.388ValAla: 6.388 ± 0.041	1.113ValCys: 1.113 ± 0.015	4.293ValAsp: 4.293 ± 0.03	4.212ValGlu: 4.212 ± 0.03	2.595ValPhe: 2.595 ± 0.025	4.389ValGly: 4.389 ± 0.033	1.7ValHis: 1.7 ± 0.02	2.62ValIle: 2.62 ± 0.026	2.731ValLys: 2.731 ± 0.025	6.837ValLeu: 6.837 ± 0.039	1.407ValMet: 1.407 ± 0.018	2.235ValAsn: 2.235 ± 0.024	3.758ValPro: 3.758 ± 0.034	2.791ValGln: 2.791 ± 0.02	3.86ValArg: 3.86 ± 0.028	5.402ValSer: 5.402 ± 0.039	3.993ValThr: 3.993 ± 0.032	5.294ValVal: 5.294 ± 0.045	0.943ValTrp: 0.943 ± 0.016	1.778ValTyr: 1.778 ± 0.02	0.0ValXaa: 0.0 ± 0.0
Trp	0.884TrpAla: 0.884 ± 0.016	0.242TrpCys: 0.242 ± 0.008	0.802TrpAsp: 0.802 ± 0.015	0.78TrpGlu: 0.78 ± 0.014	0.491TrpPhe: 0.491 ± 0.01	0.768TrpGly: 0.768 ± 0.013	0.334TrpHis: 0.334 ± 0.009	0.732TrpIle: 0.732 ± 0.012	0.87TrpLys: 0.87 ± 0.014	1.231TrpLeu: 1.231 ± 0.019	0.404TrpMet: 0.404 ± 0.011	0.708TrpAsn: 0.708 ± 0.014	0.467TrpPro: 0.467 ± 0.01	0.599TrpGln: 0.599 ± 0.011	0.853TrpArg: 0.853 ± 0.014	1.016TrpSer: 1.016 ± 0.018	0.93TrpThr: 0.93 ± 0.014	0.713TrpVal: 0.713 ± 0.012	0.239TrpTrp: 0.239 ± 0.008	0.409TrpTyr: 0.409 ± 0.009	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.001TyrAla: 2.001 ± 0.021	0.486TyrCys: 0.486 ± 0.011	1.676TyrAsp: 1.676 ± 0.019	1.539TyrGlu: 1.539 ± 0.02	1.061TyrPhe: 1.061 ± 0.016	1.957TyrGly: 1.957 ± 0.022	0.73TyrHis: 0.73 ± 0.015	1.066TyrIle: 1.066 ± 0.017	1.01TyrLys: 1.01 ± 0.018	2.351TyrLeu: 2.351 ± 0.024	0.562TyrMet: 0.562 ± 0.013	1.046TyrAsn: 1.046 ± 0.017	1.172TyrPro: 1.172 ± 0.017	1.102TyrGln: 1.102 ± 0.018	1.689TyrArg: 1.689 ± 0.021	1.826TyrSer: 1.826 ± 0.021	1.419TyrThr: 1.419 ± 0.019	1.709TyrVal: 1.709 ± 0.019	0.382TyrTrp: 0.382 ± 0.009	0.772TyrTyr: 0.772 ± 0.013	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 10465 proteins (4838762 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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