Lactobacillus prophage Lj965
Average proteome isoelectric point is 6.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q6SE96|Q6SE96_9CAUD Uncharacterized protein OS=Lactobacillus prophage Lj965 OX=139870 GN=Ljo_0296b PE=4 SV=1
MM1 pKa = 7.77 KK2 pKa = 10.4 EE3 pKa = 3.69 FDD5 pKa = 5.68 PRR7 pKa = 11.84 DD8 pKa = 3.25 LWKK11 pKa = 10.42 LQEE14 pKa = 4.14 VNGMVLRR21 pKa = 11.84 DD22 pKa = 3.16 IHH24 pKa = 7.53 GIDD27 pKa = 3.38 VAIGKK32 pKa = 9.63 GFEE35 pKa = 4.16 YY36 pKa = 10.89 KK37 pKa = 10.42 NIKK40 pKa = 10.31 AFIEE44 pKa = 4.81 VYY46 pKa = 6.45 TTEE49 pKa = 4.24 YY50 pKa = 10.34 GVKK53 pKa = 10.56 DD54 pKa = 4.08 FMEE57 pKa = 4.38 KK58 pKa = 9.55 MGFEE62 pKa = 3.99 NSEE65 pKa = 4.54 DD66 pKa = 3.29 FTKK69 pKa = 10.98 YY70 pKa = 10.23 YY71 pKa = 10.66 FKK73 pKa = 10.76 EE74 pKa = 4.45 FPDD77 pKa = 3.43 EE78 pKa = 4.29 CDD80 pKa = 3.52 WYY82 pKa = 10.41 DD83 pKa = 3.1 ACYY86 pKa = 9.32 WAFNGIYY93 pKa = 10.3 ADD95 pKa = 4.31 DD96 pKa = 4.53 LALKK100 pKa = 10.24 GYY102 pKa = 9.15 EE103 pKa = 3.87 EE104 pKa = 4.17 EE105 pKa = 5.07 AYY107 pKa = 10.98 LDD109 pKa = 4.07 AEE111 pKa = 4.22 DD112 pKa = 4.44 AKK114 pKa = 10.79 RR115 pKa = 11.84 DD116 pKa = 3.81 RR117 pKa = 11.84 LAGKK121 pKa = 9.73
Molecular weight: 14.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.384
IPC2_protein 4.418
IPC_protein 4.342
Toseland 4.177
ProMoST 4.469
Dawson 4.304
Bjellqvist 4.457
Wikipedia 4.19
Rodwell 4.177
Grimsley 4.075
Solomon 4.304
Lehninger 4.253
Nozaki 4.406
DTASelect 4.596
Thurlkill 4.19
EMBOSS 4.202
Sillero 4.457
Patrickios 3.516
IPC_peptide 4.304
IPC2_peptide 4.444
IPC2.peptide.svr19 4.396
Protein with the highest isoelectric point:
>tr|Q6SE82|Q6SE82_9CAUD Uncharacterized protein OS=Lactobacillus prophage Lj965 OX=139870 GN=Ljo_0310 PE=4 SV=1
MM1 pKa = 7.46 KK2 pKa = 10.42 VNKK5 pKa = 10.08 KK6 pKa = 10.33 KK7 pKa = 9.25 FTYY10 pKa = 8.48 WQLRR14 pKa = 11.84 DD15 pKa = 3.71 LQDD18 pKa = 3.23 EE19 pKa = 4.14 QRR21 pKa = 11.84 NQDD24 pKa = 3.34 EE25 pKa = 4.13 ATEE28 pKa = 3.94 RR29 pKa = 11.84 LKK31 pKa = 10.72 IINNAYY37 pKa = 10.12 QKK39 pKa = 10.14 AQSYY43 pKa = 10.86 LSDD46 pKa = 3.85 EE47 pKa = 4.07 VQKK50 pKa = 10.48 IYY52 pKa = 10.87 RR53 pKa = 11.84 RR54 pKa = 11.84 YY55 pKa = 9.46 FYY57 pKa = 11.46 ADD59 pKa = 2.82 ISKK62 pKa = 11.19 DD63 pKa = 3.42 EE64 pKa = 4.26 VATIMSSHH72 pKa = 7.08 ISPSEE77 pKa = 3.77 LVTLKK82 pKa = 10.78 ALSSSITDD90 pKa = 3.4 KK91 pKa = 10.93 EE92 pKa = 4.08 SRR94 pKa = 11.84 QAVDD98 pKa = 3.25 NYY100 pKa = 10.8 LSRR103 pKa = 11.84 LAAKK107 pKa = 10.3 SRR109 pKa = 11.84 ITRR112 pKa = 11.84 LEE114 pKa = 3.68 EE115 pKa = 3.91 MQLKK119 pKa = 10.42 AYY121 pKa = 9.57 IAAKK125 pKa = 10.08 SAGATEE131 pKa = 4.8 LDD133 pKa = 3.92 QNVKK137 pKa = 10.15 LHH139 pKa = 5.8 TDD141 pKa = 2.49 IMKK144 pKa = 10.36 RR145 pKa = 11.84 AWSEE149 pKa = 3.99 AEE151 pKa = 4.12 KK152 pKa = 10.79 QSAVYY157 pKa = 8.24 DD158 pKa = 3.7 TTKK161 pKa = 10.84 DD162 pKa = 3.64 YY163 pKa = 10.28 TLHH166 pKa = 6.46 SPHH169 pKa = 7.08 SVEE172 pKa = 4.17 VKK174 pKa = 9.58 QDD176 pKa = 3.35 KK177 pKa = 10.57 IVIKK181 pKa = 11.0 NPDD184 pKa = 3.33 TGKK187 pKa = 10.49 EE188 pKa = 4.14 VATVPMDD195 pKa = 3.62 KK196 pKa = 10.52 DD197 pKa = 3.86 VPKK200 pKa = 10.99 SKK202 pKa = 8.96 ITEE205 pKa = 3.76 IPNRR209 pKa = 11.84 YY210 pKa = 8.47 VEE212 pKa = 4.54 KK213 pKa = 10.73 SLEE216 pKa = 4.21 TRR218 pKa = 11.84 WKK220 pKa = 10.16 GKK222 pKa = 9.96 NFSARR227 pKa = 11.84 IWGNTDD233 pKa = 2.67 KK234 pKa = 11.03 LAEE237 pKa = 4.17 RR238 pKa = 11.84 LQEE241 pKa = 4.02 LFTVKK246 pKa = 10.08 EE247 pKa = 3.97 LSNLPEE253 pKa = 4.95 RR254 pKa = 11.84 EE255 pKa = 3.85 MIKK258 pKa = 10.33 RR259 pKa = 11.84 IEE261 pKa = 3.84 QEE263 pKa = 3.92 FNVGKK268 pKa = 10.02 FYY270 pKa = 11.07 ASRR273 pKa = 11.84 LIRR276 pKa = 11.84 TEE278 pKa = 4.25 ANFFYY283 pKa = 11.13 SKK285 pKa = 10.52 IKK287 pKa = 10.06 LDD289 pKa = 3.25 NWRR292 pKa = 11.84 KK293 pKa = 10.06 RR294 pKa = 11.84 GVKK297 pKa = 9.51 QYY299 pKa = 11.32 QLLAVIDD306 pKa = 3.92 SRR308 pKa = 11.84 TSKK311 pKa = 9.87 ICRR314 pKa = 11.84 SINGNIYY321 pKa = 9.51 NVKK324 pKa = 10.43 DD325 pKa = 3.37 AVFGKK330 pKa = 9.85 NVPPLHH336 pKa = 6.12 PFCRR340 pKa = 11.84 TVPVIYY346 pKa = 10.43 LGNARR351 pKa = 11.84 SANNKK356 pKa = 8.44 PVKK359 pKa = 10.15 KK360 pKa = 10.59
Molecular weight: 41.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.985
IPC2_protein 9.033
IPC_protein 8.916
Toseland 9.794
ProMoST 9.472
Dawson 9.999
Bjellqvist 9.633
Wikipedia 10.145
Rodwell 10.467
Grimsley 10.058
Solomon 10.014
Lehninger 9.984
Nozaki 9.78
DTASelect 9.633
Thurlkill 9.838
EMBOSS 10.189
Sillero 9.911
Patrickios 9.692
IPC_peptide 10.014
IPC2_peptide 8.068
IPC2.peptide.svr19 7.992
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
46
0
46
11916
49
2021
259.0
29.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.276 ± 0.474
0.529 ± 0.126
6.596 ± 0.369
6.185 ± 0.389
4.087 ± 0.27
6.16 ± 0.685
1.435 ± 0.172
6.63 ± 0.225
8.87 ± 0.568
8.124 ± 0.318
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.266 ± 0.216
6.781 ± 0.435
2.87 ± 0.212
4.397 ± 0.232
4.045 ± 0.217
6.73 ± 0.359
5.975 ± 0.426
5.715 ± 0.228
1.502 ± 0.146
3.827 ± 0.376
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here