Lactobacillus prophage Lj965

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6SE96|Q6SE96_9CAUD Uncharacterized protein OS=Lactobacillus prophage Lj965 OX=139870 GN=Ljo_0296b PE=4 SV=1
MM1 pKa = 7.77KK2 pKa = 10.4EE3 pKa = 3.69FDD5 pKa = 5.68PRR7 pKa = 11.84DD8 pKa = 3.25LWKK11 pKa = 10.42LQEE14 pKa = 4.14VNGMVLRR21 pKa = 11.84DD22 pKa = 3.16IHH24 pKa = 7.53GIDD27 pKa = 3.38VAIGKK32 pKa = 9.63GFEE35 pKa = 4.16YY36 pKa = 10.89KK37 pKa = 10.42NIKK40 pKa = 10.31AFIEE44 pKa = 4.81VYY46 pKa = 6.45TTEE49 pKa = 4.24YY50 pKa = 10.34GVKK53 pKa = 10.56DD54 pKa = 4.08FMEE57 pKa = 4.38KK58 pKa = 9.55MGFEE62 pKa = 3.99NSEE65 pKa = 4.54DD66 pKa = 3.29FTKK69 pKa = 10.98YY70 pKa = 10.23YY71 pKa = 10.66FKK73 pKa = 10.76EE74 pKa = 4.45FPDD77 pKa = 3.43EE78 pKa = 4.29CDD80 pKa = 3.52WYY82 pKa = 10.41DD83 pKa = 3.1ACYY86 pKa = 9.32WAFNGIYY93 pKa = 10.3ADD95 pKa = 4.31DD96 pKa = 4.53LALKK100 pKa = 10.24GYY102 pKa = 9.15EE103 pKa = 3.87EE104 pKa = 4.17EE105 pKa = 5.07AYY107 pKa = 10.98LDD109 pKa = 4.07AEE111 pKa = 4.22DD112 pKa = 4.44AKK114 pKa = 10.79RR115 pKa = 11.84DD116 pKa = 3.81RR117 pKa = 11.84LAGKK121 pKa = 9.73

Molecular weight: 14.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6SE82|Q6SE82_9CAUD Uncharacterized protein OS=Lactobacillus prophage Lj965 OX=139870 GN=Ljo_0310 PE=4 SV=1
MM1 pKa = 7.46KK2 pKa = 10.42VNKK5 pKa = 10.08KK6 pKa = 10.33KK7 pKa = 9.25FTYY10 pKa = 8.48WQLRR14 pKa = 11.84DD15 pKa = 3.71LQDD18 pKa = 3.23EE19 pKa = 4.14QRR21 pKa = 11.84NQDD24 pKa = 3.34EE25 pKa = 4.13ATEE28 pKa = 3.94RR29 pKa = 11.84LKK31 pKa = 10.72IINNAYY37 pKa = 10.12QKK39 pKa = 10.14AQSYY43 pKa = 10.86LSDD46 pKa = 3.85EE47 pKa = 4.07VQKK50 pKa = 10.48IYY52 pKa = 10.87RR53 pKa = 11.84RR54 pKa = 11.84YY55 pKa = 9.46FYY57 pKa = 11.46ADD59 pKa = 2.82ISKK62 pKa = 11.19DD63 pKa = 3.42EE64 pKa = 4.26VATIMSSHH72 pKa = 7.08ISPSEE77 pKa = 3.77LVTLKK82 pKa = 10.78ALSSSITDD90 pKa = 3.4KK91 pKa = 10.93EE92 pKa = 4.08SRR94 pKa = 11.84QAVDD98 pKa = 3.25NYY100 pKa = 10.8LSRR103 pKa = 11.84LAAKK107 pKa = 10.3SRR109 pKa = 11.84ITRR112 pKa = 11.84LEE114 pKa = 3.68EE115 pKa = 3.91MQLKK119 pKa = 10.42AYY121 pKa = 9.57IAAKK125 pKa = 10.08SAGATEE131 pKa = 4.8LDD133 pKa = 3.92QNVKK137 pKa = 10.15LHH139 pKa = 5.8TDD141 pKa = 2.49IMKK144 pKa = 10.36RR145 pKa = 11.84AWSEE149 pKa = 3.99AEE151 pKa = 4.12KK152 pKa = 10.79QSAVYY157 pKa = 8.24DD158 pKa = 3.7TTKK161 pKa = 10.84DD162 pKa = 3.64YY163 pKa = 10.28TLHH166 pKa = 6.46SPHH169 pKa = 7.08SVEE172 pKa = 4.17VKK174 pKa = 9.58QDD176 pKa = 3.35KK177 pKa = 10.57IVIKK181 pKa = 11.0NPDD184 pKa = 3.33TGKK187 pKa = 10.49EE188 pKa = 4.14VATVPMDD195 pKa = 3.62KK196 pKa = 10.52DD197 pKa = 3.86VPKK200 pKa = 10.99SKK202 pKa = 8.96ITEE205 pKa = 3.76IPNRR209 pKa = 11.84YY210 pKa = 8.47VEE212 pKa = 4.54KK213 pKa = 10.73SLEE216 pKa = 4.21TRR218 pKa = 11.84WKK220 pKa = 10.16GKK222 pKa = 9.96NFSARR227 pKa = 11.84IWGNTDD233 pKa = 2.67KK234 pKa = 11.03LAEE237 pKa = 4.17RR238 pKa = 11.84LQEE241 pKa = 4.02LFTVKK246 pKa = 10.08EE247 pKa = 3.97LSNLPEE253 pKa = 4.95RR254 pKa = 11.84EE255 pKa = 3.85MIKK258 pKa = 10.33RR259 pKa = 11.84IEE261 pKa = 3.84QEE263 pKa = 3.92FNVGKK268 pKa = 10.02FYY270 pKa = 11.07ASRR273 pKa = 11.84LIRR276 pKa = 11.84TEE278 pKa = 4.25ANFFYY283 pKa = 11.13SKK285 pKa = 10.52IKK287 pKa = 10.06LDD289 pKa = 3.25NWRR292 pKa = 11.84KK293 pKa = 10.06RR294 pKa = 11.84GVKK297 pKa = 9.51QYY299 pKa = 11.32QLLAVIDD306 pKa = 3.92SRR308 pKa = 11.84TSKK311 pKa = 9.87ICRR314 pKa = 11.84SINGNIYY321 pKa = 9.51NVKK324 pKa = 10.43DD325 pKa = 3.37AVFGKK330 pKa = 9.85NVPPLHH336 pKa = 6.12PFCRR340 pKa = 11.84TVPVIYY346 pKa = 10.43LGNARR351 pKa = 11.84SANNKK356 pKa = 8.44PVKK359 pKa = 10.15KK360 pKa = 10.59

Molecular weight: 41.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

46

0

46

11916

49

2021

259.0

29.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.276 ± 0.474

0.529 ± 0.126

6.596 ± 0.369

6.185 ± 0.389

4.087 ± 0.27

6.16 ± 0.685

1.435 ± 0.172

6.63 ± 0.225

8.87 ± 0.568

8.124 ± 0.318

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.266 ± 0.216

6.781 ± 0.435

2.87 ± 0.212

4.397 ± 0.232

4.045 ± 0.217

6.73 ± 0.359

5.975 ± 0.426

5.715 ± 0.228

1.502 ± 0.146

3.827 ± 0.376
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski