Amino acid dipepetide frequency for Pseudorhodoferax sp. Leaf274

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.74AlaAla: 21.74 ± 0.166	1.455AlaCys: 1.455 ± 0.027	7.149AlaAsp: 7.149 ± 0.072	7.248AlaGlu: 7.248 ± 0.075	4.315AlaPhe: 4.315 ± 0.054	12.69AlaGly: 12.69 ± 0.092	2.99AlaHis: 2.99 ± 0.044	5.15AlaIle: 5.15 ± 0.05	3.404AlaLys: 3.404 ± 0.057	17.479AlaLeu: 17.479 ± 0.139	3.85AlaMet: 3.85 ± 0.049	2.706AlaAsn: 2.706 ± 0.045	7.541AlaPro: 7.541 ± 0.084	7.069AlaGln: 7.069 ± 0.073	10.712AlaArg: 10.712 ± 0.094	6.835AlaSer: 6.835 ± 0.063	6.621AlaThr: 6.621 ± 0.063	9.959AlaVal: 9.959 ± 0.084	2.163AlaTrp: 2.163 ± 0.037	2.583AlaTyr: 2.583 ± 0.035	0.0AlaXaa: 0.0 ± 0.0
Cys	1.278CysAla: 1.278 ± 0.029	0.119CysCys: 0.119 ± 0.009	0.494CysAsp: 0.494 ± 0.016	0.457CysGlu: 0.457 ± 0.016	0.285CysPhe: 0.285 ± 0.012	0.985CysGly: 0.985 ± 0.022	0.234CysHis: 0.234 ± 0.011	0.377CysIle: 0.377 ± 0.015	0.189CysLys: 0.189 ± 0.009	0.795CysLeu: 0.795 ± 0.02	0.212CysMet: 0.212 ± 0.011	0.211CysAsn: 0.211 ± 0.01	0.414CysPro: 0.414 ± 0.016	0.243CysGln: 0.243 ± 0.01	0.578CysArg: 0.578 ± 0.02	0.455CysSer: 0.455 ± 0.014	0.502CysThr: 0.502 ± 0.015	0.717CysVal: 0.717 ± 0.02	0.138CysTrp: 0.138 ± 0.008	0.176CysTyr: 0.176 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	8.074AspAla: 8.074 ± 0.078	0.457AspCys: 0.457 ± 0.019	2.589AspAsp: 2.589 ± 0.037	2.761AspGlu: 2.761 ± 0.044	1.955AspPhe: 1.955 ± 0.034	4.921AspGly: 4.921 ± 0.063	1.119AspHis: 1.119 ± 0.023	2.224AspIle: 2.224 ± 0.036	1.491AspLys: 1.491 ± 0.031	5.356AspLeu: 5.356 ± 0.062	1.17AspMet: 1.17 ± 0.026	1.15AspAsn: 1.15 ± 0.028	2.935AspPro: 2.935 ± 0.041	1.641AspGln: 1.641 ± 0.029	3.321AspArg: 3.321 ± 0.044	2.045AspSer: 2.045 ± 0.035	2.524AspThr: 2.524 ± 0.038	3.839AspVal: 3.839 ± 0.046	0.981AspTrp: 0.981 ± 0.024	1.259AspTyr: 1.259 ± 0.025	0.0AspXaa: 0.0 ± 0.0
Glu	6.779GluAla: 6.779 ± 0.07	0.316GluCys: 0.316 ± 0.011	2.111GluAsp: 2.111 ± 0.036	2.23GluGlu: 2.23 ± 0.043	1.589GluPhe: 1.589 ± 0.03	3.588GluGly: 3.588 ± 0.043	1.27GluHis: 1.27 ± 0.027	2.324GluIle: 2.324 ± 0.041	1.423GluLys: 1.423 ± 0.033	5.512GluLeu: 5.512 ± 0.061	1.113GluMet: 1.113 ± 0.027	1.061GluAsn: 1.061 ± 0.023	2.454GluPro: 2.454 ± 0.031	2.562GluGln: 2.562 ± 0.045	4.676GluArg: 4.676 ± 0.06	2.082GluSer: 2.082 ± 0.035	2.256GluThr: 2.256 ± 0.038	3.744GluVal: 3.744 ± 0.048	0.615GluTrp: 0.615 ± 0.017	0.877GluTyr: 0.877 ± 0.021	0.0GluXaa: 0.0 ± 0.0
Phe	4.464PheAla: 4.464 ± 0.054	0.366PheCys: 0.366 ± 0.014	2.455PheAsp: 2.455 ± 0.035	1.871PheGlu: 1.871 ± 0.031	1.194PhePhe: 1.194 ± 0.031	3.445PheGly: 3.445 ± 0.049	0.705PheHis: 0.705 ± 0.019	1.245PheIle: 1.245 ± 0.029	1.015PheLys: 1.015 ± 0.026	2.79PheLeu: 2.79 ± 0.038	0.753PheMet: 0.753 ± 0.018	0.937PheAsn: 0.937 ± 0.027	1.425PhePro: 1.425 ± 0.026	1.07PheGln: 1.07 ± 0.022	1.795PheArg: 1.795 ± 0.029	1.937PheSer: 1.937 ± 0.034	1.829PheThr: 1.829 ± 0.035	2.75PheVal: 2.75 ± 0.044	0.494PheTrp: 0.494 ± 0.015	0.766PheTyr: 0.766 ± 0.02	0.0PheXaa: 0.0 ± 0.0
Gly	10.611GlyAla: 10.611 ± 0.081	0.882GlyCys: 0.882 ± 0.022	3.92GlyAsp: 3.92 ± 0.047	4.322GlyGlu: 4.322 ± 0.052	3.272GlyPhe: 3.272 ± 0.044	7.466GlyGly: 7.466 ± 0.075	1.991GlyHis: 1.991 ± 0.034	3.861GlyIle: 3.861 ± 0.047	2.871GlyLys: 2.871 ± 0.046	9.704GlyLeu: 9.704 ± 0.074	2.465GlyMet: 2.465 ± 0.039	2.081GlyAsn: 2.081 ± 0.043	3.544GlyPro: 3.544 ± 0.046	3.862GlyGln: 3.862 ± 0.053	6.133GlyArg: 6.133 ± 0.063	4.625GlySer: 4.625 ± 0.056	4.775GlyThr: 4.775 ± 0.054	6.732GlyVal: 6.732 ± 0.059	1.597GlyTrp: 1.597 ± 0.03	2.356GlyTyr: 2.356 ± 0.038	0.0GlyXaa: 0.0 ± 0.0
His	3.365HisAla: 3.365 ± 0.05	0.283HisCys: 0.283 ± 0.011	1.214HisAsp: 1.214 ± 0.028	1.052HisGlu: 1.052 ± 0.024	0.824HisPhe: 0.824 ± 0.02	2.341HisGly: 2.341 ± 0.035	0.606HisHis: 0.606 ± 0.021	0.855HisIle: 0.855 ± 0.023	0.524HisLys: 0.524 ± 0.017	2.235HisLeu: 2.235 ± 0.033	0.465HisMet: 0.465 ± 0.015	0.448HisAsn: 0.448 ± 0.016	1.481HisPro: 1.481 ± 0.029	0.757HisGln: 0.757 ± 0.021	1.55HisArg: 1.55 ± 0.031	0.883HisSer: 0.883 ± 0.022	0.985HisThr: 0.985 ± 0.024	1.646HisVal: 1.646 ± 0.029	0.433HisTrp: 0.433 ± 0.015	0.577HisTyr: 0.577 ± 0.018	0.0HisXaa: 0.0 ± 0.0
Ile	6.342IleAla: 6.342 ± 0.058	0.338IleCys: 0.338 ± 0.013	2.711IleAsp: 2.711 ± 0.05	2.458IleGlu: 2.458 ± 0.038	1.014IlePhe: 1.014 ± 0.028	3.998IleGly: 3.998 ± 0.042	0.714IleHis: 0.714 ± 0.019	0.985IleIle: 0.985 ± 0.024	1.079IleLys: 1.079 ± 0.028	2.843IleLeu: 2.843 ± 0.044	0.635IleMet: 0.635 ± 0.018	1.072IleAsn: 1.072 ± 0.026	1.757IlePro: 1.757 ± 0.035	1.109IleGln: 1.109 ± 0.026	2.208IleArg: 2.208 ± 0.038	1.855IleSer: 1.855 ± 0.034	2.137IleThr: 2.137 ± 0.037	3.444IleVal: 3.444 ± 0.046	0.399IleTrp: 0.399 ± 0.015	0.794IleTyr: 0.794 ± 0.021	0.0IleXaa: 0.0 ± 0.0
Lys	3.562LysAla: 3.562 ± 0.06	0.117LysCys: 0.117 ± 0.007	1.394LysAsp: 1.394 ± 0.029	1.153LysGlu: 1.153 ± 0.026	0.783LysPhe: 0.783 ± 0.022	2.157LysGly: 2.157 ± 0.04	0.485LysHis: 0.485 ± 0.015	1.169LysIle: 1.169 ± 0.025	1.055LysLys: 1.055 ± 0.027	2.943LysLeu: 2.943 ± 0.045	0.632LysMet: 0.632 ± 0.019	0.71LysAsn: 0.71 ± 0.02	1.837LysPro: 1.837 ± 0.032	0.956LysGln: 0.956 ± 0.021	1.788LysArg: 1.788 ± 0.033	1.369LysSer: 1.369 ± 0.025	1.638LysThr: 1.638 ± 0.031	2.043LysVal: 2.043 ± 0.041	0.283LysTrp: 0.283 ± 0.012	0.521LysTyr: 0.521 ± 0.017	0.0LysXaa: 0.0 ± 0.0
Leu	17.764LeuAla: 17.764 ± 0.141	1.021LeuCys: 1.021 ± 0.024	6.059LeuAsp: 6.059 ± 0.061	4.776LeuGlu: 4.776 ± 0.059	3.287LeuPhe: 3.287 ± 0.044	9.199LeuGly: 9.199 ± 0.088	2.528LeuHis: 2.528 ± 0.042	3.392LeuIle: 3.392 ± 0.048	2.84LeuLys: 2.84 ± 0.045	12.227LeuLeu: 12.227 ± 0.13	2.283LeuMet: 2.283 ± 0.035	2.416LeuAsn: 2.416 ± 0.044	6.653LeuPro: 6.653 ± 0.07	5.145LeuGln: 5.145 ± 0.058	8.588LeuArg: 8.588 ± 0.087	5.527LeuSer: 5.527 ± 0.054	4.898LeuThr: 4.898 ± 0.049	8.749LeuVal: 8.749 ± 0.083	1.388LeuTrp: 1.388 ± 0.03	2.01LeuTyr: 2.01 ± 0.032	0.002LeuXaa: 0.002 ± 0.001
Met	3.45MetAla: 3.45 ± 0.052	0.164MetCys: 0.164 ± 0.009	1.166MetAsp: 1.166 ± 0.025	0.933MetGlu: 0.933 ± 0.022	0.676MetPhe: 0.676 ± 0.019	1.867MetGly: 1.867 ± 0.034	0.555MetHis: 0.555 ± 0.016	0.638MetIle: 0.638 ± 0.021	0.735MetLys: 0.735 ± 0.02	2.712MetLeu: 2.712 ± 0.039	0.451MetMet: 0.451 ± 0.016	0.709MetAsn: 0.709 ± 0.019	1.572MetPro: 1.572 ± 0.028	1.152MetGln: 1.152 ± 0.024	1.755MetArg: 1.755 ± 0.03	1.322MetSer: 1.322 ± 0.026	1.373MetThr: 1.373 ± 0.025	1.83MetVal: 1.83 ± 0.034	0.188MetTrp: 0.188 ± 0.01	0.341MetTyr: 0.341 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	3.186AsnAla: 3.186 ± 0.054	0.223AsnCys: 0.223 ± 0.011	1.119AsnAsp: 1.119 ± 0.026	0.943AsnGlu: 0.943 ± 0.022	0.859AsnPhe: 0.859 ± 0.023	2.054AsnGly: 2.054 ± 0.044	0.439AsnHis: 0.439 ± 0.013	1.026AsnIle: 1.026 ± 0.025	0.68AsnLys: 0.68 ± 0.021	2.386AsnLeu: 2.386 ± 0.035	0.52AsnMet: 0.52 ± 0.018	0.633AsnAsn: 0.633 ± 0.021	1.616AsnPro: 1.616 ± 0.032	0.767AsnGln: 0.767 ± 0.024	1.467AsnArg: 1.467 ± 0.031	0.951AsnSer: 0.951 ± 0.025	1.27AsnThr: 1.27 ± 0.028	1.742AsnVal: 1.742 ± 0.034	0.373AsnTrp: 0.373 ± 0.016	0.608AsnTyr: 0.608 ± 0.02	0.0AsnXaa: 0.0 ± 0.0
Pro	8.61ProAla: 8.61 ± 0.083	0.338ProCys: 0.338 ± 0.013	3.171ProAsp: 3.171 ± 0.049	3.086ProGlu: 3.086 ± 0.042	1.81ProPhe: 1.81 ± 0.032	5.08ProGly: 5.08 ± 0.057	1.212ProHis: 1.212 ± 0.025	1.774ProIle: 1.774 ± 0.029	1.241ProLys: 1.241 ± 0.024	5.733ProLeu: 5.733 ± 0.054	1.299ProMet: 1.299 ± 0.026	1.149ProAsn: 1.149 ± 0.024	3.001ProPro: 3.001 ± 0.052	2.397ProGln: 2.397 ± 0.034	3.411ProArg: 3.411 ± 0.043	2.59ProSer: 2.59 ± 0.037	2.615ProThr: 2.615 ± 0.042	4.407ProVal: 4.407 ± 0.054	0.875ProTrp: 0.875 ± 0.023	1.217ProTyr: 1.217 ± 0.025	0.0ProXaa: 0.0 ± 0.0
Gln	7.273GlnAla: 7.273 ± 0.079	0.286GlnCys: 0.286 ± 0.013	1.813GlnAsp: 1.813 ± 0.031	1.568GlnGlu: 1.568 ± 0.027	1.217GlnPhe: 1.217 ± 0.021	3.506GlnGly: 3.506 ± 0.043	1.058GlnHis: 1.058 ± 0.021	1.65GlnIle: 1.65 ± 0.027	0.876GlnLys: 0.876 ± 0.022	4.797GlnLeu: 4.797 ± 0.056	0.97GlnMet: 0.97 ± 0.021	0.795GlnAsn: 0.795 ± 0.022	2.603GlnPro: 2.603 ± 0.043	2.235GlnGln: 2.235 ± 0.04	3.906GlnArg: 3.906 ± 0.049	1.869GlnSer: 1.869 ± 0.031	1.794GlnThr: 1.794 ± 0.033	3.33GlnVal: 3.33 ± 0.043	0.671GlnTrp: 0.671 ± 0.018	0.728GlnTyr: 0.728 ± 0.022	0.0GlnXaa: 0.0 ± 0.0
Arg	9.258ArgAla: 9.258 ± 0.089	0.61ArgCys: 0.61 ± 0.018	3.568ArgAsp: 3.568 ± 0.044	3.977ArgGlu: 3.977 ± 0.05	2.768ArgPhe: 2.768 ± 0.038	5.024ArgGly: 5.024 ± 0.056	2.018ArgHis: 2.018 ± 0.034	3.382ArgIle: 3.382 ± 0.037	1.878ArgLys: 1.878 ± 0.036	8.535ArgLeu: 8.535 ± 0.078	2.014ArgMet: 2.014 ± 0.034	1.676ArgAsn: 1.676 ± 0.031	3.628ArgPro: 3.628 ± 0.045	3.262ArgGln: 3.262 ± 0.05	5.558ArgArg: 5.558 ± 0.071	3.552ArgSer: 3.552 ± 0.042	3.434ArgThr: 3.434 ± 0.042	5.3ArgVal: 5.3 ± 0.051	1.33ArgTrp: 1.33 ± 0.027	1.764ArgTyr: 1.764 ± 0.027	0.0ArgXaa: 0.0 ± 0.0
Ser	6.522SerAla: 6.522 ± 0.061	0.409SerCys: 0.409 ± 0.015	2.313SerAsp: 2.313 ± 0.039	2.092SerGlu: 2.092 ± 0.032	1.955SerPhe: 1.955 ± 0.033	4.966SerGly: 4.966 ± 0.06	1.025SerHis: 1.025 ± 0.023	2.051SerIle: 2.051 ± 0.035	1.217SerLys: 1.217 ± 0.028	5.048SerLeu: 5.048 ± 0.053	1.214SerMet: 1.214 ± 0.024	1.202SerAsn: 1.202 ± 0.029	2.623SerPro: 2.623 ± 0.035	1.617SerGln: 1.617 ± 0.028	3.143SerArg: 3.143 ± 0.044	2.647SerSer: 2.647 ± 0.034	2.755SerThr: 2.755 ± 0.042	3.817SerVal: 3.817 ± 0.044	0.704SerTrp: 0.704 ± 0.02	1.143SerTyr: 1.143 ± 0.025	0.0SerXaa: 0.0 ± 0.001
Thr	6.514ThrAla: 6.514 ± 0.057	0.342ThrCys: 0.342 ± 0.013	2.428ThrAsp: 2.428 ± 0.039	2.214ThrGlu: 2.214 ± 0.036	1.444ThrPhe: 1.444 ± 0.029	4.773ThrGly: 4.773 ± 0.051	1.073ThrHis: 1.073 ± 0.022	1.832ThrIle: 1.832 ± 0.035	1.046ThrLys: 1.046 ± 0.027	6.085ThrLeu: 6.085 ± 0.071	1.079ThrMet: 1.079 ± 0.026	1.024ThrAsn: 1.024 ± 0.028	3.652ThrPro: 3.652 ± 0.049	1.979ThrGln: 1.979 ± 0.032	3.326ThrArg: 3.326 ± 0.043	2.33ThrSer: 2.33 ± 0.036	2.701ThrThr: 2.701 ± 0.048	4.405ThrVal: 4.405 ± 0.063	0.646ThrTrp: 0.646 ± 0.017	0.937ThrTyr: 0.937 ± 0.022	0.0ThrXaa: 0.0 ± 0.0
Val	10.599ValAla: 10.599 ± 0.084	0.731ValCys: 0.731 ± 0.019	4.28ValAsp: 4.28 ± 0.052	3.915ValGlu: 3.915 ± 0.054	2.691ValPhe: 2.691 ± 0.042	5.924ValGly: 5.924 ± 0.059	1.703ValHis: 1.703 ± 0.033	2.682ValIle: 2.682 ± 0.04	2.024ValLys: 2.024 ± 0.04	9.24ValLeu: 9.24 ± 0.08	1.67ValMet: 1.67 ± 0.032	1.91ValAsn: 1.91 ± 0.04	4.446ValPro: 4.446 ± 0.05	3.476ValGln: 3.476 ± 0.044	5.687ValArg: 5.687 ± 0.056	3.73ValSer: 3.73 ± 0.045	3.747ValThr: 3.747 ± 0.045	6.791ValVal: 6.791 ± 0.069	0.952ValTrp: 0.952 ± 0.022	1.571ValTyr: 1.571 ± 0.028	0.0ValXaa: 0.0 ± 0.001
Trp	1.519TrpAla: 1.519 ± 0.03	0.177TrpCys: 0.177 ± 0.009	0.656TrpAsp: 0.656 ± 0.019	0.515TrpGlu: 0.515 ± 0.015	0.556TrpPhe: 0.556 ± 0.017	1.017TrpGly: 1.017 ± 0.025	0.393TrpHis: 0.393 ± 0.015	0.606TrpIle: 0.606 ± 0.018	0.382TrpLys: 0.382 ± 0.012	2.062TrpLeu: 2.062 ± 0.04	0.398TrpMet: 0.398 ± 0.015	0.459TrpAsn: 0.459 ± 0.019	0.81TrpPro: 0.81 ± 0.022	0.827TrpGln: 0.827 ± 0.02	1.309TrpArg: 1.309 ± 0.029	0.81TrpSer: 0.81 ± 0.017	0.798TrpThr: 0.798 ± 0.018	0.991TrpVal: 0.991 ± 0.023	0.308TrpTrp: 0.308 ± 0.013	0.296TrpTyr: 0.296 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.749TyrAla: 2.749 ± 0.036	0.226TyrCys: 0.226 ± 0.011	1.203TyrAsp: 1.203 ± 0.027	1.01TyrGlu: 1.01 ± 0.024	0.812TyrPhe: 0.812 ± 0.019	2.07TyrGly: 2.07 ± 0.032	0.407TyrHis: 0.407 ± 0.014	0.67TyrIle: 0.67 ± 0.018	0.627TyrLys: 0.627 ± 0.022	2.269TyrLeu: 2.269 ± 0.034	0.395TyrMet: 0.395 ± 0.015	0.512TyrAsn: 0.512 ± 0.018	1.024TyrPro: 1.024 ± 0.025	0.818TyrGln: 0.818 ± 0.02	1.624TyrArg: 1.624 ± 0.031	1.047TyrSer: 1.047 ± 0.026	1.177TyrThr: 1.177 ± 0.032	1.533TyrVal: 1.533 ± 0.03	0.35TyrTrp: 0.35 ± 0.014	0.519TyrTyr: 0.519 ± 0.019	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.001	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.001	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5992 proteins (2002829 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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