Pygoscelis adeliae papillomavirus 2
Average proteome isoelectric point is 6.5
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A291PWL4|A0A291PWL4_9PAPI E6 OS=Pygoscelis adeliae papillomavirus 2 OX=2045113 GN=E6 PE=4 SV=1
MM1 pKa = 7.61 FEE3 pKa = 5.1 RR4 pKa = 11.84 DD5 pKa = 3.66 PTDD8 pKa = 3.02 MRR10 pKa = 11.84 PAHH13 pKa = 6.78 LGDD16 pKa = 3.94 TNITTYY22 pKa = 11.09 EE23 pKa = 4.09 EE24 pKa = 4.07 LLIDD28 pKa = 4.9 LMYY31 pKa = 8.93 PTDD34 pKa = 4.28 EE35 pKa = 4.54 DD36 pKa = 3.99 VEE38 pKa = 4.58 SQSEE42 pKa = 4.23 SDD44 pKa = 3.74 SDD46 pKa = 4.71 IEE48 pKa = 4.7 GLTYY52 pKa = 10.89 DD53 pKa = 3.9 CTTSEE58 pKa = 4.77 LYY60 pKa = 9.13 CTEE63 pKa = 4.61 TIDD66 pKa = 5.37 SEE68 pKa = 5.09 SEE70 pKa = 3.77 DD71 pKa = 3.6 GNAVDD76 pKa = 4.62 GQVSIPAPSGPPEE89 pKa = 4.25 SLSLEE94 pKa = 4.07 QLSVEE99 pKa = 4.3 EE100 pKa = 4.34 FLEE103 pKa = 4.05 EE104 pKa = 5.37 AIDD107 pKa = 4.11 GFYY110 pKa = 7.92 PTPTGWTCHH119 pKa = 5.68 YY120 pKa = 9.59 CDD122 pKa = 4.66 SEE124 pKa = 4.26 LSPVEE129 pKa = 4.33 VAAHH133 pKa = 6.61 GPTDD137 pKa = 3.13 PWNRR141 pKa = 11.84 TGLCNMCYY149 pKa = 10.13 CSGLDD154 pKa = 3.63 DD155 pKa = 4.98 LFSTEE160 pKa = 3.94 EE161 pKa = 3.79
Molecular weight: 17.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.026
IPC2_protein 3.427
IPC_protein 3.401
Toseland 3.21
ProMoST 3.554
Dawson 3.401
Bjellqvist 3.592
Wikipedia 3.312
Rodwell 3.236
Grimsley 3.121
Solomon 3.376
Lehninger 3.325
Nozaki 3.516
DTASelect 3.694
Thurlkill 3.261
EMBOSS 3.325
Sillero 3.528
Patrickios 0.006
IPC_peptide 3.363
IPC2_peptide 3.503
IPC2.peptide.svr19 3.619
Protein with the highest isoelectric point:
>tr|A0A291PWK7|A0A291PWK7_9PAPI E1 OS=Pygoscelis adeliae papillomavirus 2 OX=2045113 GN=E1 PE=4 SV=1
MM1 pKa = 7.06 PTIYY5 pKa = 10.45 CPNFQTCWLVNPPDD19 pKa = 4.04 GVSTQGMTSVTITCRR34 pKa = 11.84 TEE36 pKa = 3.7 SQSYY40 pKa = 6.06 QTCSLAIVLFLKK52 pKa = 10.65 KK53 pKa = 10.26 AGQGLHH59 pKa = 6.54 LNCIPPALPALRR71 pKa = 11.84 RR72 pKa = 11.84 RR73 pKa = 11.84 LLGRR77 pKa = 11.84 SYY79 pKa = 10.8 RR80 pKa = 11.84 GRR82 pKa = 11.84 LDD84 pKa = 3.24 ARR86 pKa = 11.84 LEE88 pKa = 3.99 EE89 pKa = 4.32 LADD92 pKa = 4.05 LGWRR96 pKa = 11.84 IEE98 pKa = 4.1 YY99 pKa = 10.32 LLKK102 pKa = 9.71 TQLPFNTATIMMPTEE117 pKa = 4.14 NLLDD121 pKa = 4.25 LYY123 pKa = 10.87 SYY125 pKa = 10.8 LGPTPDD131 pKa = 3.45 EE132 pKa = 4.21 EE133 pKa = 5.76 AEE135 pKa = 4.15 LRR137 pKa = 11.84 HH138 pKa = 5.34 QVHH141 pKa = 5.96 VMLLEE146 pKa = 4.99 AGMKK150 pKa = 9.98 RR151 pKa = 11.84 KK152 pKa = 8.99 QRR154 pKa = 11.84 HH155 pKa = 4.45 LRR157 pKa = 11.84 RR158 pKa = 11.84 GRR160 pKa = 11.84 LSGNSFFNASS170 pKa = 3.1
Molecular weight: 19.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.697
IPC2_protein 8.492
IPC_protein 8.551
Toseland 8.624
ProMoST 8.858
Dawson 9.107
Bjellqvist 9.268
Wikipedia 9.194
Rodwell 9.151
Grimsley 8.653
Solomon 9.297
Lehninger 9.268
Nozaki 9.399
DTASelect 9.063
Thurlkill 9.107
EMBOSS 9.268
Sillero 9.37
Patrickios 4.507
IPC_peptide 9.282
IPC2_peptide 8.536
IPC2.peptide.svr19 8.018
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2610
99
690
372.9
41.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.594 ± 0.475
2.222 ± 0.508
6.054 ± 0.499
6.705 ± 0.806
3.142 ± 0.311
7.318 ± 0.724
1.801 ± 0.098
4.1 ± 0.322
3.602 ± 0.616
9.732 ± 0.861
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.648 ± 0.212
3.793 ± 0.661
7.625 ± 1.027
3.64 ± 0.367
7.318 ± 0.503
8.276 ± 0.345
7.011 ± 0.575
5.441 ± 0.864
1.379 ± 0.111
3.602 ± 0.364
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here