Pygoscelis adeliae papillomavirus 2 
Average proteome isoelectric point is 6.5 
Get precalculated fractions of proteins 
 
  
    Acidic  
 
  
    pI < 6.8  
 
  
    6.8-7.4  
 
  
    pI > 7.4  
 
  
    Basic  
    
 
  
    All  
 
Note: above files contain also dissociation constants (pKa) 
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 Summary statistics related to proteome-wise predictions 
Protein with the lowest isoelectric point: 
>tr|A0A291PWL4|A0A291PWL4_9PAPI E6 OS=Pygoscelis adeliae papillomavirus 2 OX=2045113 GN=E6 PE=4 SV=1MM1 pKa = 7.61  FEE3 pKa = 5.1  RR4 pKa = 11.84  DD5 pKa = 3.66  PTDD8 pKa = 3.02  MRR10 pKa = 11.84  PAHH13 pKa = 6.78  LGDD16 pKa = 3.94  TNITTYY22 pKa = 11.09  EE23 pKa = 4.09  EE24 pKa = 4.07  LLIDD28 pKa = 4.9  LMYY31 pKa = 8.93  PTDD34 pKa = 4.28  EE35 pKa = 4.54  DD36 pKa = 3.99  VEE38 pKa = 4.58  SQSEE42 pKa = 4.23  SDD44 pKa = 3.74  SDD46 pKa = 4.71  IEE48 pKa = 4.7  GLTYY52 pKa = 10.89  DD53 pKa = 3.9  CTTSEE58 pKa = 4.77  LYY60 pKa = 9.13  CTEE63 pKa = 4.61  TIDD66 pKa = 5.37  SEE68 pKa = 5.09  SEE70 pKa = 3.77  DD71 pKa = 3.6  GNAVDD76 pKa = 4.62  GQVSIPAPSGPPEE89 pKa = 4.25  SLSLEE94 pKa = 4.07  QLSVEE99 pKa = 4.3  EE100 pKa = 4.34  FLEE103 pKa = 4.05  EE104 pKa = 5.37  AIDD107 pKa = 4.11  GFYY110 pKa = 7.92  PTPTGWTCHH119 pKa = 5.68  YY120 pKa = 9.59  CDD122 pKa = 4.66  SEE124 pKa = 4.26  LSPVEE129 pKa = 4.33  VAAHH133 pKa = 6.61  GPTDD137 pKa = 3.13  PWNRR141 pKa = 11.84  TGLCNMCYY149 pKa = 10.13  CSGLDD154 pKa = 3.63  DD155 pKa = 4.98  LFSTEE160 pKa = 3.94  EE161 pKa = 3.79  
 17.79 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  4.026 
IPC2_protein 3.427 
IPC_protein 3.401 
Toseland    3.21 
ProMoST     3.554 
Dawson      3.401 
Bjellqvist  3.592 
Wikipedia   3.312 
Rodwell     3.236 
Grimsley    3.121 
Solomon     3.376 
Lehninger   3.325 
Nozaki      3.516 
DTASelect   3.694 
Thurlkill   3.261 
EMBOSS      3.325 
Sillero     3.528 
Patrickios  0.006 
IPC_peptide 3.363 
IPC2_peptide  3.503 
IPC2.peptide.svr19  3.619 
 Protein with the highest isoelectric point: 
>tr|A0A291PWK7|A0A291PWK7_9PAPI E1 OS=Pygoscelis adeliae papillomavirus 2 OX=2045113 GN=E1 PE=4 SV=1MM1 pKa = 7.06  PTIYY5 pKa = 10.45  CPNFQTCWLVNPPDD19 pKa = 4.04  GVSTQGMTSVTITCRR34 pKa = 11.84  TEE36 pKa = 3.7  SQSYY40 pKa = 6.06  QTCSLAIVLFLKK52 pKa = 10.65  KK53 pKa = 10.26  AGQGLHH59 pKa = 6.54  LNCIPPALPALRR71 pKa = 11.84  RR72 pKa = 11.84  RR73 pKa = 11.84  LLGRR77 pKa = 11.84  SYY79 pKa = 10.8  RR80 pKa = 11.84  GRR82 pKa = 11.84  LDD84 pKa = 3.24  ARR86 pKa = 11.84  LEE88 pKa = 3.99  EE89 pKa = 4.32  LADD92 pKa = 4.05  LGWRR96 pKa = 11.84  IEE98 pKa = 4.1  YY99 pKa = 10.32  LLKK102 pKa = 9.71  TQLPFNTATIMMPTEE117 pKa = 4.14  NLLDD121 pKa = 4.25  LYY123 pKa = 10.87  SYY125 pKa = 10.8  LGPTPDD131 pKa = 3.45  EE132 pKa = 4.21  EE133 pKa = 5.76  AEE135 pKa = 4.15  LRR137 pKa = 11.84  HH138 pKa = 5.34  QVHH141 pKa = 5.96  VMLLEE146 pKa = 4.99  AGMKK150 pKa = 9.98  RR151 pKa = 11.84  KK152 pKa = 8.99  QRR154 pKa = 11.84  HH155 pKa = 4.45  LRR157 pKa = 11.84  RR158 pKa = 11.84  GRR160 pKa = 11.84  LSGNSFFNASS170 pKa = 3.1  
 19.31 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  8.697 
IPC2_protein 8.492 
IPC_protein 8.551 
Toseland    8.624 
ProMoST     8.858 
Dawson      9.107 
Bjellqvist  9.268 
Wikipedia   9.194 
Rodwell     9.151 
Grimsley    8.653 
Solomon     9.297 
Lehninger   9.268 
Nozaki      9.399 
DTASelect   9.063 
Thurlkill   9.107 
EMBOSS      9.268 
Sillero     9.37 
Patrickios  4.507 
IPC_peptide 9.282 
IPC2_peptide  8.536 
IPC2.peptide.svr19  8.018 
 Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
   
  
General Statistics 
    
      
        Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
 
     
    
      
        7 
0
7 
2610
99
690
372.9
41.61
          
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
 
     
    
                 
        5.594 ± 0.475
2.222 ± 0.508
6.054 ± 0.499
6.705 ± 0.806
3.142 ± 0.311
7.318 ± 0.724
1.801 ± 0.098
4.1 ± 0.322
3.602 ± 0.616
9.732 ± 0.861
          
     
  
  
  
      
          
        Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
 
     
    
                 
        1.648 ± 0.212
3.793 ± 0.661
7.625 ± 1.027
3.64 ± 0.367
7.318 ± 0.503
8.276 ± 0.345
7.011 ± 0.575
5.441 ± 0.864
1.379 ± 0.111
3.602 ± 0.364
          
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein levelMost of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here