Acinetobacter phage vB_AbaM_IME284
Average proteome isoelectric point is 6.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 84 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3G3BXS3|A0A3G3BXS3_9CAUD Uncharacterized protein OS=Acinetobacter phage vB_AbaM_IME284 OX=2419625 PE=4 SV=1
MM1 pKa = 7.25 AQYY4 pKa = 9.85 DD5 pKa = 3.73 IPLAQVPNQFFTTSLNGVTWSITLEE30 pKa = 3.98 TRR32 pKa = 11.84 LNNLYY37 pKa = 10.27 ISLSNNNDD45 pKa = 2.72 GDD47 pKa = 3.93 VLLNRR52 pKa = 11.84 ICLNRR57 pKa = 11.84 TYY59 pKa = 10.65 LGYY62 pKa = 11.24 GFIFVDD68 pKa = 3.2 IDD70 pKa = 3.72 GNNDD74 pKa = 3.33 PEE76 pKa = 4.4 YY77 pKa = 9.83 TGLGTRR83 pKa = 11.84 YY84 pKa = 10.14 LLIWTDD90 pKa = 3.71 EE91 pKa = 4.13 VV92 pKa = 3.51
Molecular weight: 10.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.836
IPC2_protein 3.961
IPC_protein 3.859
Toseland 3.656
ProMoST 4.05
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.846
Rodwell 3.694
Grimsley 3.567
Solomon 3.846
Lehninger 3.795
Nozaki 4.012
DTASelect 4.24
Thurlkill 3.732
EMBOSS 3.846
Sillero 3.986
Patrickios 0.401
IPC_peptide 3.834
IPC2_peptide 3.948
IPC2.peptide.svr19 3.884
Protein with the highest isoelectric point:
>tr|A0A3G3BXS6|A0A3G3BXS6_9CAUD Uncharacterized protein OS=Acinetobacter phage vB_AbaM_IME284 OX=2419625 PE=4 SV=1
MM1 pKa = 7.27 NPIAMILLSFALSFVFVVVLYY22 pKa = 10.63 LYY24 pKa = 9.64 EE25 pKa = 4.25 AYY27 pKa = 9.53 KK28 pKa = 10.26 RR29 pKa = 11.84 KK30 pKa = 10.12 RR31 pKa = 11.84 YY32 pKa = 7.46 VQRR35 pKa = 11.84 KK36 pKa = 8.76 QDD38 pKa = 3.67 DD39 pKa = 3.52 QRR41 pKa = 11.84 KK42 pKa = 8.64 FNPIRR47 pKa = 11.84 EE48 pKa = 4.3 KK49 pKa = 10.66 QWWKK53 pKa = 10.98
Molecular weight: 6.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.236
IPC2_protein 9.765
IPC_protein 10.058
Toseland 10.321
ProMoST 10.072
Dawson 10.496
Bjellqvist 10.16
Wikipedia 10.672
Rodwell 10.965
Grimsley 10.57
Solomon 10.54
Lehninger 10.511
Nozaki 10.277
DTASelect 10.16
Thurlkill 10.35
EMBOSS 10.716
Sillero 10.409
Patrickios 10.716
IPC_peptide 10.54
IPC2_peptide 8.799
IPC2.peptide.svr19 8.706
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
84
0
84
13134
37
682
156.4
17.58
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.02 ± 0.418
1.203 ± 0.133
6.061 ± 0.274
6.693 ± 0.392
4.584 ± 0.259
7.035 ± 0.305
1.705 ± 0.152
6.868 ± 0.273
7.637 ± 0.403
8.17 ± 0.29
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.81 ± 0.162
5.68 ± 0.28
3.19 ± 0.176
3.99 ± 0.234
3.571 ± 0.241
6.51 ± 0.213
5.497 ± 0.319
6.495 ± 0.281
1.515 ± 0.122
3.769 ± 0.247
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here