Fasciola gigantica (Giant liver fluke)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Spiralia; Lophotrochozoa; Platyhelminthes; Trematoda; Digenea; Plagiorchiida; Echinostomata; Echinostomatoidea; Fasciolidae; Fasciola

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12643 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A504YZH6|A0A504YZH6_FASGI Leucine rich repeat flightless interacting OS=Fasciola gigantica OX=46835 GN=FGIG_06733 PE=3 SV=1
MM1 pKa = 7.29VSSFDD6 pKa = 5.48DD7 pKa = 4.08GAPDD11 pKa = 3.45YY12 pKa = 11.02KK13 pKa = 10.73CASRR17 pKa = 11.84DD18 pKa = 3.86LLCTALDD25 pKa = 3.45QGPVYY30 pKa = 9.25EE31 pKa = 4.64ASVASEE37 pKa = 3.92CWDD40 pKa = 3.06WGLTVPLRR48 pKa = 11.84EE49 pKa = 4.18TSS51 pKa = 3.11

Molecular weight: 5.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A504YQA8|A0A504YQA8_FASGI Uncharacterized protein OS=Fasciola gigantica OX=46835 GN=FGIG_04674 PE=4 SV=1
MM1 pKa = 6.63STGRR5 pKa = 11.84GGSMGPNTRR14 pKa = 11.84GGVGGRR20 pKa = 11.84GGRR23 pKa = 11.84GGMGPGGRR31 pKa = 11.84GGGTSMPRR39 pKa = 11.84RR40 pKa = 11.84GQQ42 pKa = 3.15

Molecular weight: 3.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12643

0

12643

5783738

24

14570

457.5

51.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.697 ± 0.02

2.095 ± 0.013

5.187 ± 0.018

5.738 ± 0.028

3.67 ± 0.017

5.484 ± 0.028

2.774 ± 0.011

4.752 ± 0.015

4.346 ± 0.02

9.666 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.123 ± 0.008

4.316 ± 0.012

5.925 ± 0.027

4.363 ± 0.017

6.493 ± 0.019

9.696 ± 0.037

6.555 ± 0.019

6.365 ± 0.015

1.139 ± 0.007

2.609 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski