Microbacterium phage Mashley

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Squashvirus; unclassified Squashvirus

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 103 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B8WGA7|A0A5B8WGA7_9CAUD Uncharacterized protein OS=Microbacterium phage Mashley OX=2603261 GN=94 PE=4 SV=1
MM1 pKa = 7.55SRR3 pKa = 11.84STWVDD8 pKa = 3.34RR9 pKa = 11.84GAPAAVILAAIIFGGVVALADD30 pKa = 4.22LADD33 pKa = 4.56EE34 pKa = 4.69PLAPCTPTSTSVCSGPPADD53 pKa = 4.44HH54 pKa = 6.66SPNGGEE60 pKa = 4.12YY61 pKa = 10.5DD62 pKa = 3.94SLPPCLTEE70 pKa = 6.3DD71 pKa = 4.24SDD73 pKa = 4.13NCVWDD78 pKa = 3.71ARR80 pKa = 11.84VDD82 pKa = 3.76GNGEE86 pKa = 3.67GRR88 pKa = 11.84SFVTIDD94 pKa = 3.34GVTHH98 pKa = 6.1YY99 pKa = 10.97LPP101 pKa = 4.59

Molecular weight: 10.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B8WJ75|A0A5B8WJ75_9CAUD Portal protein OS=Microbacterium phage Mashley OX=2603261 GN=20 PE=4 SV=1
MM1 pKa = 7.75AKK3 pKa = 9.59QEE5 pKa = 4.24RR6 pKa = 11.84PRR8 pKa = 11.84YY9 pKa = 6.64TPPRR13 pKa = 11.84LGQRR17 pKa = 11.84DD18 pKa = 3.91FARR21 pKa = 11.84TTMMPRR27 pKa = 11.84WFTRR31 pKa = 11.84EE32 pKa = 3.6AVEE35 pKa = 4.88AFGLNPYY42 pKa = 10.38DD43 pKa = 3.54CTTFAIVADD52 pKa = 4.03NLDD55 pKa = 3.7AAGVSQTATTLIASRR70 pKa = 11.84GGMSRR75 pKa = 11.84GAAARR80 pKa = 11.84AIDD83 pKa = 4.26RR84 pKa = 11.84LIANQLLFEE93 pKa = 5.58LDD95 pKa = 3.38PRR97 pKa = 11.84KK98 pKa = 9.93NGHH101 pKa = 4.93MMRR104 pKa = 11.84YY105 pKa = 9.52AIPADD110 pKa = 3.93LPWTGGRR117 pKa = 11.84RR118 pKa = 3.47

Molecular weight: 13.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

103

0

103

19643

29

1104

190.7

20.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.897 ± 0.312

0.83 ± 0.11

6.542 ± 0.223

6.633 ± 0.383

2.739 ± 0.129

8.598 ± 0.272

1.807 ± 0.17

4.867 ± 0.14

2.708 ± 0.224

7.417 ± 0.238

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.276 ± 0.131

2.922 ± 0.182

5.758 ± 0.358

3.64 ± 0.324

7.03 ± 0.255

5.432 ± 0.324

7.015 ± 0.386

7.458 ± 0.191

1.929 ± 0.137

2.5 ± 0.158
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski