Amino acid dipepetide frequency for Massilia sp. Leaf139

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.272AlaAla: 19.272 ± 0.191	1.279AlaCys: 1.279 ± 0.031	6.473AlaAsp: 6.473 ± 0.073	6.667AlaGlu: 6.667 ± 0.084	4.209AlaPhe: 4.209 ± 0.051	11.331AlaGly: 11.331 ± 0.12	2.583AlaHis: 2.583 ± 0.044	5.611AlaIle: 5.611 ± 0.068	4.07AlaLys: 4.07 ± 0.073	14.746AlaLeu: 14.746 ± 0.136	3.337AlaMet: 3.337 ± 0.046	3.539AlaAsn: 3.539 ± 0.061	7.057AlaPro: 7.057 ± 0.085	5.463AlaGln: 5.463 ± 0.075	9.369AlaArg: 9.369 ± 0.101	6.911AlaSer: 6.911 ± 0.071	6.193AlaThr: 6.193 ± 0.082	8.664AlaVal: 8.664 ± 0.075	1.752AlaTrp: 1.752 ± 0.044	2.68AlaTyr: 2.68 ± 0.046	0.001AlaXaa: 0.001 ± 0.001
Cys	1.236CysAla: 1.236 ± 0.03	0.103CysCys: 0.103 ± 0.009	0.471CysAsp: 0.471 ± 0.016	0.444CysGlu: 0.444 ± 0.019	0.277CysPhe: 0.277 ± 0.013	0.888CysGly: 0.888 ± 0.029	0.213CysHis: 0.213 ± 0.011	0.364CysIle: 0.364 ± 0.016	0.241CysLys: 0.241 ± 0.014	0.776CysLeu: 0.776 ± 0.026	0.187CysMet: 0.187 ± 0.012	0.202CysAsn: 0.202 ± 0.013	0.409CysPro: 0.409 ± 0.019	0.232CysGln: 0.232 ± 0.013	0.522CysArg: 0.522 ± 0.019	0.459CysSer: 0.459 ± 0.019	0.42CysThr: 0.42 ± 0.019	0.593CysVal: 0.593 ± 0.024	0.122CysTrp: 0.122 ± 0.01	0.201CysTyr: 0.201 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	7.298AspAla: 7.298 ± 0.071	0.432AspCys: 0.432 ± 0.018	2.774AspAsp: 2.774 ± 0.051	3.284AspGlu: 3.284 ± 0.051	2.095AspPhe: 2.095 ± 0.039	4.853AspGly: 4.853 ± 0.077	0.927AspHis: 0.927 ± 0.024	2.671AspIle: 2.671 ± 0.049	1.992AspLys: 1.992 ± 0.043	5.202AspLeu: 5.202 ± 0.074	1.29AspMet: 1.29 ± 0.031	1.436AspAsn: 1.436 ± 0.027	3.021AspPro: 3.021 ± 0.044	1.531AspGln: 1.531 ± 0.032	3.034AspArg: 3.034 ± 0.047	2.534AspSer: 2.534 ± 0.045	2.7AspThr: 2.7 ± 0.041	3.79AspVal: 3.79 ± 0.053	0.909AspTrp: 0.909 ± 0.027	1.603AspTyr: 1.603 ± 0.034	0.001AspXaa: 0.001 ± 0.001
Glu	7.001GluAla: 7.001 ± 0.093	0.365GluCys: 0.365 ± 0.017	2.309GluAsp: 2.309 ± 0.043	3.07GluGlu: 3.07 ± 0.051	1.833GluPhe: 1.833 ± 0.04	3.96GluGly: 3.96 ± 0.055	1.361GluHis: 1.361 ± 0.031	2.78GluIle: 2.78 ± 0.054	2.024GluLys: 2.024 ± 0.045	6.067GluLeu: 6.067 ± 0.079	1.405GluMet: 1.405 ± 0.029	1.531GluAsn: 1.531 ± 0.033	2.354GluPro: 2.354 ± 0.04	2.693GluGln: 2.693 ± 0.049	5.035GluArg: 5.035 ± 0.073	2.48GluSer: 2.48 ± 0.037	2.784GluThr: 2.784 ± 0.045	3.901GluVal: 3.901 ± 0.052	0.681GluTrp: 0.681 ± 0.022	1.155GluTyr: 1.155 ± 0.03	0.001GluXaa: 0.001 ± 0.001
Phe	4.433PheAla: 4.433 ± 0.05	0.35PheCys: 0.35 ± 0.015	2.588PheAsp: 2.588 ± 0.049	2.095PheGlu: 2.095 ± 0.037	1.334PhePhe: 1.334 ± 0.035	3.514PheGly: 3.514 ± 0.046	0.686PheHis: 0.686 ± 0.021	1.578PheIle: 1.578 ± 0.031	1.268PheLys: 1.268 ± 0.034	3.062PheLeu: 3.062 ± 0.054	0.824PheMet: 0.824 ± 0.026	1.192PheAsn: 1.192 ± 0.033	1.499PhePro: 1.499 ± 0.033	1.025PheGln: 1.025 ± 0.027	1.857PheArg: 1.857 ± 0.035	2.136PheSer: 2.136 ± 0.038	2.07PheThr: 2.07 ± 0.04	2.736PheVal: 2.736 ± 0.044	0.456PheTrp: 0.456 ± 0.019	0.944PheTyr: 0.944 ± 0.024	0.0PheXaa: 0.0 ± 0.0
Gly	10.474GlyAla: 10.474 ± 0.115	0.764GlyCys: 0.764 ± 0.027	4.086GlyAsp: 4.086 ± 0.06	4.617GlyGlu: 4.617 ± 0.055	3.306GlyPhe: 3.306 ± 0.044	7.159GlyGly: 7.159 ± 0.102	1.776GlyHis: 1.776 ± 0.037	4.125GlyIle: 4.125 ± 0.053	3.638GlyLys: 3.638 ± 0.061	8.168GlyLeu: 8.168 ± 0.079	2.506GlyMet: 2.506 ± 0.044	2.421GlyAsn: 2.421 ± 0.055	2.834GlyPro: 2.834 ± 0.048	3.235GlyGln: 3.235 ± 0.055	5.451GlyArg: 5.451 ± 0.063	4.84GlySer: 4.84 ± 0.079	4.609GlyThr: 4.609 ± 0.082	6.318GlyVal: 6.318 ± 0.063	1.337GlyTrp: 1.337 ± 0.034	2.376GlyTyr: 2.376 ± 0.043	0.0GlyXaa: 0.0 ± 0.0
His	2.821HisAla: 2.821 ± 0.051	0.237HisCys: 0.237 ± 0.013	1.182HisAsp: 1.182 ± 0.033	1.148HisGlu: 1.148 ± 0.034	0.855HisPhe: 0.855 ± 0.024	2.069HisGly: 2.069 ± 0.039	0.578HisHis: 0.578 ± 0.023	0.916HisIle: 0.916 ± 0.028	0.598HisLys: 0.598 ± 0.018	2.061HisLeu: 2.061 ± 0.036	0.524HisMet: 0.524 ± 0.017	0.527HisAsn: 0.527 ± 0.022	1.323HisPro: 1.323 ± 0.032	0.64HisGln: 0.64 ± 0.02	1.277HisArg: 1.277 ± 0.031	0.983HisSer: 0.983 ± 0.025	1.043HisThr: 1.043 ± 0.03	1.429HisVal: 1.429 ± 0.03	0.312HisTrp: 0.312 ± 0.014	0.644HisTyr: 0.644 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	6.238IleAla: 6.238 ± 0.068	0.367IleCys: 0.367 ± 0.014	3.377IleAsp: 3.377 ± 0.054	3.245IleGlu: 3.245 ± 0.046	1.275IlePhe: 1.275 ± 0.032	4.557IleGly: 4.557 ± 0.057	0.851IleHis: 0.851 ± 0.025	1.7IleIle: 1.7 ± 0.038	1.623IleLys: 1.623 ± 0.038	3.761IleLeu: 3.761 ± 0.055	0.861IleMet: 0.861 ± 0.027	1.52IleAsn: 1.52 ± 0.032	2.099IlePro: 2.099 ± 0.036	1.09IleGln: 1.09 ± 0.026	2.629IleArg: 2.629 ± 0.047	2.337IleSer: 2.337 ± 0.041	2.398IleThr: 2.398 ± 0.043	3.923IleVal: 3.923 ± 0.06	0.421IleTrp: 0.421 ± 0.016	0.95IleTyr: 0.95 ± 0.027	0.0IleXaa: 0.0 ± 0.0
Lys	4.088LysAla: 4.088 ± 0.069	0.157LysCys: 0.157 ± 0.01	1.717LysAsp: 1.717 ± 0.037	1.82LysGlu: 1.82 ± 0.046	1.014LysPhe: 1.014 ± 0.03	2.45LysGly: 2.45 ± 0.041	0.733LysHis: 0.733 ± 0.023	1.716LysIle: 1.716 ± 0.04	1.538LysLys: 1.538 ± 0.041	3.857LysLeu: 3.857 ± 0.051	0.97LysMet: 0.97 ± 0.025	1.207LysAsn: 1.207 ± 0.033	2.144LysPro: 2.144 ± 0.043	1.291LysGln: 1.291 ± 0.031	2.394LysArg: 2.394 ± 0.047	1.753LysSer: 1.753 ± 0.036	2.075LysThr: 2.075 ± 0.042	2.543LysVal: 2.543 ± 0.046	0.368LysTrp: 0.368 ± 0.02	0.751LysTyr: 0.751 ± 0.024	0.001LysXaa: 0.001 ± 0.001
Leu	15.386LeuAla: 15.386 ± 0.136	0.967LeuCys: 0.967 ± 0.026	6.155LeuAsp: 6.155 ± 0.073	5.347LeuGlu: 5.347 ± 0.069	3.543LeuPhe: 3.543 ± 0.057	8.293LeuGly: 8.293 ± 0.072	2.217LeuHis: 2.217 ± 0.042	4.072LeuIle: 4.072 ± 0.053	3.428LeuLys: 3.428 ± 0.053	10.905LeuLeu: 10.905 ± 0.134	2.335LeuMet: 2.335 ± 0.041	2.842LeuAsn: 2.842 ± 0.042	5.867LeuPro: 5.867 ± 0.063	3.606LeuGln: 3.606 ± 0.05	7.758LeuArg: 7.758 ± 0.084	5.813LeuSer: 5.813 ± 0.06	5.183LeuThr: 5.183 ± 0.067	7.833LeuVal: 7.833 ± 0.082	1.091LeuTrp: 1.091 ± 0.028	2.183LeuTyr: 2.183 ± 0.037	0.001LeuXaa: 0.001 ± 0.001
Met	3.051MetAla: 3.051 ± 0.045	0.15MetCys: 0.15 ± 0.011	1.112MetAsp: 1.112 ± 0.027	1.17MetGlu: 1.17 ± 0.024	0.801MetPhe: 0.801 ± 0.022	1.736MetGly: 1.736 ± 0.039	0.549MetHis: 0.549 ± 0.019	1.069MetIle: 1.069 ± 0.026	1.071MetLys: 1.071 ± 0.029	2.788MetLeu: 2.788 ± 0.047	0.64MetMet: 0.64 ± 0.026	0.983MetAsn: 0.983 ± 0.027	1.427MetPro: 1.427 ± 0.027	1.026MetGln: 1.026 ± 0.026	1.881MetArg: 1.881 ± 0.031	1.531MetSer: 1.531 ± 0.034	1.521MetThr: 1.521 ± 0.028	1.699MetVal: 1.699 ± 0.041	0.21MetTrp: 0.21 ± 0.012	0.481MetTyr: 0.481 ± 0.02	0.0MetXaa: 0.0 ± 0.0
Asn	3.743AsnAla: 3.743 ± 0.066	0.274AsnCys: 0.274 ± 0.014	1.459AsnAsp: 1.459 ± 0.033	1.481AsnGlu: 1.481 ± 0.031	1.046AsnPhe: 1.046 ± 0.029	2.767AsnGly: 2.767 ± 0.048	0.564AsnHis: 0.564 ± 0.02	1.401AsnIle: 1.401 ± 0.033	1.036AsnLys: 1.036 ± 0.029	2.936AsnLeu: 2.936 ± 0.053	0.679AsnMet: 0.679 ± 0.022	1.009AsnAsn: 1.009 ± 0.033	1.907AsnPro: 1.907 ± 0.038	0.934AsnGln: 0.934 ± 0.024	1.87AsnArg: 1.87 ± 0.035	1.309AsnSer: 1.309 ± 0.034	1.575AsnThr: 1.575 ± 0.037	2.228AsnVal: 2.228 ± 0.044	0.423AsnTrp: 0.423 ± 0.019	0.812AsnTyr: 0.812 ± 0.025	0.0AsnXaa: 0.0 ± 0.0
Pro	7.24ProAla: 7.24 ± 0.091	0.319ProCys: 0.319 ± 0.015	3.189ProAsp: 3.189 ± 0.05	3.32ProGlu: 3.32 ± 0.053	1.773ProPhe: 1.773 ± 0.035	4.597ProGly: 4.597 ± 0.067	1.075ProHis: 1.075 ± 0.03	1.887ProIle: 1.887 ± 0.037	1.565ProLys: 1.565 ± 0.036	5.118ProLeu: 5.118 ± 0.069	1.136ProMet: 1.136 ± 0.024	1.471ProAsn: 1.471 ± 0.03	2.498ProPro: 2.498 ± 0.058	2.051ProGln: 2.051 ± 0.037	2.782ProArg: 2.782 ± 0.045	2.534ProSer: 2.534 ± 0.041	2.346ProThr: 2.346 ± 0.042	4.162ProVal: 4.162 ± 0.054	0.596ProTrp: 0.596 ± 0.022	1.176ProTyr: 1.176 ± 0.029	0.0ProXaa: 0.0 ± 0.0
Gln	5.064GlnAla: 5.064 ± 0.072	0.241GlnCys: 0.241 ± 0.013	1.617GlnAsp: 1.617 ± 0.031	1.754GlnGlu: 1.754 ± 0.046	1.35GlnPhe: 1.35 ± 0.027	2.726GlnGly: 2.726 ± 0.044	0.857GlnHis: 0.857 ± 0.025	1.668GlnIle: 1.668 ± 0.035	1.17GlnLys: 1.17 ± 0.028	4.075GlnLeu: 4.075 ± 0.054	0.999GlnMet: 0.999 ± 0.028	0.962GlnAsn: 0.962 ± 0.027	2.003GlnPro: 2.003 ± 0.038	1.738GlnGln: 1.738 ± 0.039	2.76GlnArg: 2.76 ± 0.043	1.796GlnSer: 1.796 ± 0.036	1.789GlnThr: 1.789 ± 0.038	3.027GlnVal: 3.027 ± 0.046	0.422GlnTrp: 0.422 ± 0.018	0.86GlnTyr: 0.86 ± 0.024	0.0GlnXaa: 0.0 ± 0.0
Arg	8.292ArgAla: 8.292 ± 0.096	0.494ArgCys: 0.494 ± 0.022	3.607ArgAsp: 3.607 ± 0.049	4.145ArgGlu: 4.145 ± 0.063	2.854ArgPhe: 2.854 ± 0.042	4.585ArgGly: 4.585 ± 0.055	1.742ArgHis: 1.742 ± 0.036	3.656ArgIle: 3.656 ± 0.052	2.195ArgLys: 2.195 ± 0.041	7.493ArgLeu: 7.493 ± 0.086	1.946ArgMet: 1.946 ± 0.036	1.992ArgAsn: 1.992 ± 0.034	3.023ArgPro: 3.023 ± 0.048	2.806ArgGln: 2.806 ± 0.046	4.933ArgArg: 4.933 ± 0.071	3.408ArgSer: 3.408 ± 0.044	3.313ArgThr: 3.313 ± 0.049	4.917ArgVal: 4.917 ± 0.061	0.951ArgTrp: 0.951 ± 0.028	1.95ArgTyr: 1.95 ± 0.045	0.0ArgXaa: 0.0 ± 0.0
Ser	6.252SerAla: 6.252 ± 0.069	0.434SerCys: 0.434 ± 0.018	2.567SerAsp: 2.567 ± 0.05	2.532SerGlu: 2.532 ± 0.038	2.178SerPhe: 2.178 ± 0.041	5.283SerGly: 5.283 ± 0.075	1.077SerHis: 1.077 ± 0.027	2.495SerIle: 2.495 ± 0.04	1.784SerLys: 1.784 ± 0.034	5.616SerLeu: 5.616 ± 0.06	1.381SerMet: 1.381 ± 0.029	1.65SerAsn: 1.65 ± 0.035	2.513SerPro: 2.513 ± 0.04	1.766SerGln: 1.766 ± 0.038	3.151SerArg: 3.151 ± 0.049	3.174SerSer: 3.174 ± 0.067	2.989SerThr: 2.989 ± 0.051	3.866SerVal: 3.866 ± 0.048	0.739SerTrp: 0.739 ± 0.024	1.426SerTyr: 1.426 ± 0.035	0.001SerXaa: 0.001 ± 0.001
Thr	5.974ThrAla: 5.974 ± 0.069	0.367ThrCys: 0.367 ± 0.017	2.561ThrAsp: 2.561 ± 0.049	2.482ThrGlu: 2.482 ± 0.043	1.726ThrPhe: 1.726 ± 0.038	4.835ThrGly: 4.835 ± 0.083	1.046ThrHis: 1.046 ± 0.028	2.565ThrIle: 2.565 ± 0.046	1.457ThrLys: 1.457 ± 0.037	6.116ThrLeu: 6.116 ± 0.081	1.238ThrMet: 1.238 ± 0.027	1.451ThrAsn: 1.451 ± 0.04	3.365ThrPro: 3.365 ± 0.059	1.72ThrGln: 1.72 ± 0.031	3.482ThrArg: 3.482 ± 0.061	2.685ThrSer: 2.685 ± 0.041	2.858ThrThr: 2.858 ± 0.052	4.426ThrVal: 4.426 ± 0.065	0.655ThrTrp: 0.655 ± 0.02	1.167ThrTyr: 1.167 ± 0.035	0.0ThrXaa: 0.0 ± 0.0
Val	9.374ValAla: 9.374 ± 0.085	0.648ValCys: 0.648 ± 0.022	4.145ValAsp: 4.145 ± 0.057	4.342ValGlu: 4.342 ± 0.056	2.659ValPhe: 2.659 ± 0.039	5.495ValGly: 5.495 ± 0.07	1.481ValHis: 1.481 ± 0.029	3.412ValIle: 3.412 ± 0.047	2.63ValLys: 2.63 ± 0.042	7.938ValLeu: 7.938 ± 0.08	1.766ValMet: 1.766 ± 0.033	2.337ValAsn: 2.337 ± 0.037	3.751ValPro: 3.751 ± 0.052	2.495ValGln: 2.495 ± 0.04	5.143ValArg: 5.143 ± 0.062	4.136ValSer: 4.136 ± 0.059	4.289ValThr: 4.289 ± 0.067	5.893ValVal: 5.893 ± 0.085	0.84ValTrp: 0.84 ± 0.024	1.688ValTyr: 1.688 ± 0.043	0.001ValXaa: 0.001 ± 0.001
Trp	1.143TrpAla: 1.143 ± 0.034	0.16TrpCys: 0.16 ± 0.012	0.601TrpAsp: 0.601 ± 0.02	0.58TrpGlu: 0.58 ± 0.023	0.534TrpPhe: 0.534 ± 0.022	0.812TrpGly: 0.812 ± 0.025	0.342TrpHis: 0.342 ± 0.016	0.651TrpIle: 0.651 ± 0.019	0.466TrpLys: 0.466 ± 0.019	1.629TrpLeu: 1.629 ± 0.038	0.389TrpMet: 0.389 ± 0.019	0.476TrpAsn: 0.476 ± 0.019	0.585TrpPro: 0.585 ± 0.022	0.608TrpGln: 0.608 ± 0.019	1.123TrpArg: 1.123 ± 0.028	0.742TrpSer: 0.742 ± 0.022	0.671TrpThr: 0.671 ± 0.028	0.803TrpVal: 0.803 ± 0.022	0.212TrpTrp: 0.212 ± 0.013	0.333TrpTyr: 0.333 ± 0.016	0.001TrpXaa: 0.001 ± 0.001
Tyr	2.827TyrAla: 2.827 ± 0.049	0.25TyrCys: 0.25 ± 0.013	1.385TyrAsp: 1.385 ± 0.041	1.246TyrGlu: 1.246 ± 0.029	0.985TyrPhe: 0.985 ± 0.026	2.128TyrGly: 2.128 ± 0.042	0.507TyrHis: 0.507 ± 0.02	0.885TyrIle: 0.885 ± 0.026	0.794TyrLys: 0.794 ± 0.024	2.511TyrLeu: 2.511 ± 0.042	0.484TyrMet: 0.484 ± 0.016	0.706TyrAsn: 0.706 ± 0.026	1.157TyrPro: 1.157 ± 0.03	0.925TyrGln: 0.925 ± 0.026	1.847TyrArg: 1.847 ± 0.032	1.318TyrSer: 1.318 ± 0.035	1.35TyrThr: 1.35 ± 0.042	1.711TyrVal: 1.711 ± 0.029	0.363TyrTrp: 0.363 ± 0.018	0.662TyrTyr: 0.662 ± 0.025	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.001XaaAsn: 0.001 ± 0.001	0.001XaaPro: 0.001 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.001	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.001XaaVal: 0.001 ± 0.001	0.001XaaTrp: 0.001 ± 0.001	0.001XaaTyr: 0.001 ± 0.001	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4544 proteins (1539745 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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