Ctenophore-associated circular virus 2
Average proteome isoelectric point is 7.62
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A141MJA8|A0A141MJA8_9VIRU Uncharacterized protein OS=Ctenophore-associated circular virus 2 OX=1778559 PE=4 SV=1
MM1 pKa = 8.01 PSPKK5 pKa = 9.1 STRR8 pKa = 11.84 WCFTHH13 pKa = 6.62 NNWTEE18 pKa = 3.71 PVFQQWSNLLGDD30 pKa = 3.7 EE31 pKa = 4.59 ANVKK35 pKa = 10.37 FGIIGKK41 pKa = 8.85 EE42 pKa = 3.9 VGEE45 pKa = 4.24 QGTPHH50 pKa = 6.3 LQGFLILHH58 pKa = 5.86 RR59 pKa = 11.84 QQRR62 pKa = 11.84 LSWVRR67 pKa = 11.84 RR68 pKa = 11.84 HH69 pKa = 6.21 FPDD72 pKa = 4.16 GCHH75 pKa = 5.59 WSIARR80 pKa = 11.84 SDD82 pKa = 3.77 SEE84 pKa = 4.64 TNRR87 pKa = 11.84 TYY89 pKa = 10.8 CKK91 pKa = 10.14 KK92 pKa = 10.66 DD93 pKa = 2.75 GDD95 pKa = 4.2 FIEE98 pKa = 5.64 FGVFPDD104 pKa = 3.83 AQGKK108 pKa = 7.76 RR109 pKa = 11.84 TDD111 pKa = 3.61 LDD113 pKa = 4.1 QFIEE117 pKa = 4.01 WLDD120 pKa = 3.59 EE121 pKa = 4.24 FEE123 pKa = 4.99 SNNGRR128 pKa = 11.84 PASSPEE134 pKa = 3.53 IAKK137 pKa = 7.36 THH139 pKa = 5.86 PKK141 pKa = 9.64 MYY143 pKa = 10.01 LRR145 pKa = 11.84 YY146 pKa = 8.26 PRR148 pKa = 11.84 SVRR151 pKa = 11.84 LAKK154 pKa = 10.09 RR155 pKa = 11.84 RR156 pKa = 11.84 CALFPVQEE164 pKa = 4.52 GDD166 pKa = 3.52 LNDD169 pKa = 3.53 WQRR172 pKa = 11.84 EE173 pKa = 4.19 LEE175 pKa = 4.37 EE176 pKa = 4.85 KK177 pKa = 10.8 LGADD181 pKa = 3.34 PDD183 pKa = 3.95 DD184 pKa = 3.79 RR185 pKa = 11.84 TVLFYY190 pKa = 11.16 VDD192 pKa = 3.62 PDD194 pKa = 3.57 GGKK197 pKa = 10.12 GKK199 pKa = 7.61 TWFVRR204 pKa = 11.84 RR205 pKa = 11.84 YY206 pKa = 8.26 LTLHH210 pKa = 6.77 PNLAQALPIGQIKK223 pKa = 10.55 DD224 pKa = 3.03 MAYY227 pKa = 10.29 AVDD230 pKa = 3.42 TNARR234 pKa = 11.84 VFFVNVARR242 pKa = 11.84 SGMEE246 pKa = 3.38 FLPYY250 pKa = 10.26 RR251 pKa = 11.84 FLEE254 pKa = 4.08 MLKK257 pKa = 10.79 DD258 pKa = 3.75 RR259 pKa = 11.84 MVGSSKK265 pKa = 11.02 YY266 pKa = 8.66 EE267 pKa = 3.76 SEE269 pKa = 4.18 MKK271 pKa = 10.26 IFRR274 pKa = 11.84 HH275 pKa = 5.06 NVHH278 pKa = 5.88 VVVFSNEE285 pKa = 4.05 YY286 pKa = 9.55 PDD288 pKa = 3.95 EE289 pKa = 4.37 TKK291 pKa = 8.11 MTADD295 pKa = 3.83 RR296 pKa = 11.84 YY297 pKa = 10.78 DD298 pKa = 3.2 INTII302 pKa = 3.52
Molecular weight: 35.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.395
IPC2_protein 6.313
IPC_protein 6.478
Toseland 6.722
ProMoST 6.868
Dawson 6.854
Bjellqvist 6.766
Wikipedia 6.868
Rodwell 6.839
Grimsley 6.854
Solomon 6.868
Lehninger 6.868
Nozaki 7.088
DTASelect 7.249
Thurlkill 7.278
EMBOSS 7.278
Sillero 7.234
Patrickios 4.469
IPC_peptide 6.883
IPC2_peptide 6.956
IPC2.peptide.svr19 6.886
Protein with the highest isoelectric point:
>tr|A0A141MJA8|A0A141MJA8_9VIRU Uncharacterized protein OS=Ctenophore-associated circular virus 2 OX=1778559 PE=4 SV=1
MM1 pKa = 7.12 IQRR4 pKa = 11.84 YY5 pKa = 6.04 WRR7 pKa = 11.84 GRR9 pKa = 11.84 RR10 pKa = 11.84 NRR12 pKa = 11.84 HH13 pKa = 4.07 QARR16 pKa = 11.84 ARR18 pKa = 11.84 AQIGEE23 pKa = 4.15 RR24 pKa = 11.84 RR25 pKa = 11.84 GTATAKK31 pKa = 10.12 KK32 pKa = 9.46 HH33 pKa = 6.28 AISDD37 pKa = 4.04 TDD39 pKa = 3.31 PTAYY43 pKa = 8.14 GTRR46 pKa = 11.84 VLNSVEE52 pKa = 3.9 LTAIPSQQNQGSVDD66 pKa = 2.87 IDD68 pKa = 3.55 LRR70 pKa = 11.84 SRR72 pKa = 11.84 DD73 pKa = 3.41 LAYY76 pKa = 9.82 INGWKK81 pKa = 10.1 FCIEE85 pKa = 3.93 LRR87 pKa = 11.84 NNLTEE92 pKa = 3.96 PLYY95 pKa = 10.66 INLAVVVPKK104 pKa = 10.61 HH105 pKa = 5.84 NATIDD110 pKa = 3.42 PNIWFRR116 pKa = 11.84 GYY118 pKa = 11.04 GIDD121 pKa = 4.21 RR122 pKa = 11.84 AIAFDD127 pKa = 4.24 PSTLNSNDD135 pKa = 3.18 FHH137 pKa = 7.01 CRR139 pKa = 11.84 PLNPDD144 pKa = 3.44 AYY146 pKa = 10.26 HH147 pKa = 7.44 ILMHH151 pKa = 6.38 KK152 pKa = 10.12 RR153 pKa = 11.84 YY154 pKa = 9.37 RR155 pKa = 11.84 LNGDD159 pKa = 3.27 TDD161 pKa = 3.84 TATYY165 pKa = 10.27 AHH167 pKa = 6.45 NSGRR171 pKa = 11.84 NFMNLNLWVPLRR183 pKa = 11.84 RR184 pKa = 11.84 QIRR187 pKa = 11.84 FDD189 pKa = 3.34 EE190 pKa = 4.45 STGTGAQNGRR200 pKa = 11.84 CFFVWWCDD208 pKa = 2.94 KK209 pKa = 11.11 YY210 pKa = 10.83 MSNTGTVTTAASLQMSKK227 pKa = 10.82 RR228 pKa = 11.84 IICYY232 pKa = 9.86 FRR234 pKa = 11.84 DD235 pKa = 3.51 PRR237 pKa = 11.84 NN238 pKa = 3.11
Molecular weight: 27.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.85
IPC2_protein 9.355
IPC_protein 9.838
Toseland 9.75
ProMoST 9.692
Dawson 10.072
Bjellqvist 9.882
Wikipedia 10.321
Rodwell 10.175
Grimsley 10.189
Solomon 10.116
Lehninger 10.072
Nozaki 9.882
DTASelect 9.853
Thurlkill 9.897
EMBOSS 10.204
Sillero 10.014
Patrickios 5.181
IPC_peptide 10.116
IPC2_peptide 9.048
IPC2.peptide.svr19 8.22
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
540
238
302
270.0
31.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.296 ± 1.083
1.667 ± 0.279
6.852 ± 0.353
4.63 ± 1.624
5.185 ± 0.902
6.111 ± 0.147
2.963 ± 0.014
5.185 ± 0.988
4.444 ± 0.966
7.037 ± 0.202
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.407 ± 0.197
6.296 ± 1.353
5.0 ± 0.513
3.889 ± 0.069
9.259 ± 1.069
5.185 ± 0.092
6.111 ± 0.933
5.185 ± 0.902
2.593 ± 0.046
3.704 ± 0.32
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here