Enterobacteria phage KhF3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Ounavirinae; Felixounavirus; unclassified Felixounavirus

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 78 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A384SYQ9|A0A384SYQ9_9CAUD Uncharacterized protein OS=Enterobacteria phage KhF3 OX=1651200 PE=4 SV=1
MM1 pKa = 7.58IKK3 pKa = 10.42LNKK6 pKa = 9.18KK7 pKa = 6.72QLEE10 pKa = 4.44WIKK13 pKa = 10.94DD14 pKa = 3.66YY15 pKa = 11.37ASEE18 pKa = 4.98CGSCEE23 pKa = 4.07KK24 pKa = 10.88NHH26 pKa = 4.85VRR28 pKa = 11.84YY29 pKa = 8.55STFHH33 pKa = 5.84ATFTLYY39 pKa = 10.28ISDD42 pKa = 4.03NVLSDD47 pKa = 3.67SVEE50 pKa = 4.49DD51 pKa = 5.02GVALPNEE58 pKa = 4.63LLDD61 pKa = 3.82KK62 pKa = 10.74LAVVTGTWSEE72 pKa = 3.99EE73 pKa = 4.08DD74 pKa = 3.35GHH76 pKa = 6.2EE77 pKa = 4.44LSDD80 pKa = 4.11DD81 pKa = 3.74VVFYY85 pKa = 11.11SLEE88 pKa = 4.18NIMNPEE94 pKa = 4.39YY95 pKa = 10.77IMLMTCAQDD104 pKa = 4.41CVPLQNFIKK113 pKa = 9.16EE114 pKa = 4.04HH115 pKa = 6.53CEE117 pKa = 3.65EE118 pKa = 5.33FITKK122 pKa = 9.05QVPCQVVFEE131 pKa = 4.39

Molecular weight: 15.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A384T9E7|A0A384T9E7_9CAUD Uncharacterized protein OS=Enterobacteria phage KhF3 OX=1651200 PE=4 SV=1
MM1 pKa = 7.17VKK3 pKa = 10.18RR4 pKa = 11.84VIHH7 pKa = 5.78PARR10 pKa = 11.84AKK12 pKa = 10.67LVGAMKK18 pKa = 10.62NLQTANAQVGYY29 pKa = 9.28FQEE32 pKa = 4.38QGQHH36 pKa = 5.54SSGFSYY42 pKa = 9.87PALMYY47 pKa = 9.42LQEE50 pKa = 4.48VIGIPSASGKK60 pKa = 8.88VYY62 pKa = 10.45RR63 pKa = 11.84RR64 pKa = 11.84LFEE67 pKa = 3.61ITMMLNRR74 pKa = 11.84QTLLEE79 pKa = 4.02QTKK82 pKa = 10.77KK83 pKa = 10.71NLYY86 pKa = 9.22KK87 pKa = 10.39QLSSLNTDD95 pKa = 3.5PSNTLEE101 pKa = 4.38AFAKK105 pKa = 10.17NAQKK109 pKa = 10.56VIKK112 pKa = 10.28RR113 pKa = 11.84GFGNSAILPPNAPSTVKK130 pKa = 10.6KK131 pKa = 10.53KK132 pKa = 10.76GFNAPLVEE140 pKa = 4.31TGDD143 pKa = 3.75LRR145 pKa = 11.84DD146 pKa = 3.55NLAYY150 pKa = 9.79KK151 pKa = 10.44ISTKK155 pKa = 10.59KK156 pKa = 10.62GIKK159 pKa = 9.48KK160 pKa = 10.25

Molecular weight: 17.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

78

0

78

19768

48

906

253.4

28.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.239 ± 0.312

1.219 ± 0.127

6.328 ± 0.227

6.814 ± 0.341

4.138 ± 0.146

6.5 ± 0.242

1.948 ± 0.153

5.934 ± 0.158

7.659 ± 0.352

7.957 ± 0.131

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.813 ± 0.175

5.357 ± 0.29

3.086 ± 0.136

3.526 ± 0.229

4.239 ± 0.163

6.526 ± 0.239

6.141 ± 0.38

7.057 ± 0.197

1.224 ± 0.113

4.295 ± 0.178
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski