Streptococcus satellite phage Javan121
Average proteome isoelectric point is 6.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 28 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZDY6|A0A4D5ZDY6_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan121 OX=2558531 GN=JavanS121_0019 PE=4 SV=1
MM1 pKa = 7.55 RR2 pKa = 11.84 TFSDD6 pKa = 3.67 TPKK9 pKa = 10.21 TFTFHH14 pKa = 5.7 YY15 pKa = 8.55 TFKK18 pKa = 11.1 DD19 pKa = 3.09 FDD21 pKa = 4.09 TAQVACHH28 pKa = 6.88 AILGYY33 pKa = 7.77 MTGTYY38 pKa = 7.11 EE39 pKa = 4.76 QPVIDD44 pKa = 3.97 ATYY47 pKa = 10.82 HH48 pKa = 6.24 NDD50 pKa = 3.59 DD51 pKa = 3.72 QGGHH55 pKa = 6.59 ANQLVLEE62 pKa = 4.22 YY63 pKa = 11.31 AEE65 pKa = 3.86 NRR67 pKa = 11.84 KK68 pKa = 9.61 LNKK71 pKa = 9.07 VFKK74 pKa = 10.18 RR75 pKa = 11.84 ICDD78 pKa = 3.69 SFKK81 pKa = 10.93 DD82 pKa = 4.21 YY83 pKa = 11.52 YY84 pKa = 10.51 NQPEE88 pKa = 4.58 DD89 pKa = 3.61 MTDD92 pKa = 4.14 DD93 pKa = 4.37 EE94 pKa = 6.25 LDD96 pKa = 4.42 DD97 pKa = 3.69 MAQEE101 pKa = 4.07 NEE103 pKa = 4.3 LIKK106 pKa = 10.59 EE107 pKa = 4.24 VEE109 pKa = 4.36 EE110 pKa = 4.59 PDD112 pKa = 3.37 HH113 pKa = 6.22 QRR115 pKa = 11.84 VVSLSKK121 pKa = 9.84 STQEE125 pKa = 4.12 KK126 pKa = 10.62 VNDD129 pKa = 4.22 RR130 pKa = 11.84 DD131 pKa = 3.35 TFMAFISNHH140 pKa = 4.84 NQLAEE145 pKa = 4.24 HH146 pKa = 6.92 LSMNYY151 pKa = 10.19 KK152 pKa = 10.52 EE153 pKa = 4.5 MTPEE157 pKa = 3.92 DD158 pKa = 3.6 LGAILEE164 pKa = 4.71 SISQAFNHH172 pKa = 6.8 LYY174 pKa = 10.59 DD175 pKa = 3.68 IVVEE179 pKa = 4.3 GQLVVKK185 pKa = 10.63
Molecular weight: 21.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.497
IPC2_protein 4.558
IPC_protein 4.507
Toseland 4.355
ProMoST 4.635
Dawson 4.482
Bjellqvist 4.622
Wikipedia 4.368
Rodwell 4.355
Grimsley 4.266
Solomon 4.469
Lehninger 4.431
Nozaki 4.584
DTASelect 4.774
Thurlkill 4.368
EMBOSS 4.38
Sillero 4.635
Patrickios 3.516
IPC_peptide 4.482
IPC2_peptide 4.622
IPC2.peptide.svr19 4.578
Protein with the highest isoelectric point:
>tr|A0A4D5ZJF6|A0A4D5ZJF6_9VIRU Mutator family transposase OS=Streptococcus satellite phage Javan121 OX=2558531 GN=JavanS121_0003 PE=4 SV=1
MM1 pKa = 7.49 AGSFRR6 pKa = 11.84 QHH8 pKa = 6.21 HH9 pKa = 6.18 PAFDD13 pKa = 3.68 NMLLADD19 pKa = 3.8 DD20 pKa = 5.86 RR21 pKa = 11.84 IYY23 pKa = 11.27 KK24 pKa = 9.51 NRR26 pKa = 11.84 RR27 pKa = 11.84 PTIEE31 pKa = 3.9 QNKK34 pKa = 7.86 EE35 pKa = 3.61 QQHH38 pKa = 5.43 LKK40 pKa = 10.3 KK41 pKa = 10.5 LKK43 pKa = 8.78 KK44 pKa = 9.76 KK45 pKa = 9.78 RR46 pKa = 11.84 RR47 pKa = 11.84 KK48 pKa = 9.22 GQQ50 pKa = 3.06
Molecular weight: 6.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.256
IPC2_protein 9.809
IPC_protein 10.35
Toseland 11.052
ProMoST 10.672
Dawson 11.111
Bjellqvist 10.76
Wikipedia 11.286
Rodwell 11.506
Grimsley 11.125
Solomon 11.242
Lehninger 11.213
Nozaki 11.023
DTASelect 10.76
Thurlkill 11.023
EMBOSS 11.447
Sillero 11.038
Patrickios 11.257
IPC_peptide 11.257
IPC2_peptide 9.282
IPC2.peptide.svr19 8.721
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
28
0
28
4480
44
562
160.0
18.47
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.58 ± 0.292
0.513 ± 0.163
5.179 ± 0.379
7.857 ± 0.45
4.174 ± 0.199
5.223 ± 0.387
1.987 ± 0.222
7.411 ± 0.497
9.107 ± 0.557
9.844 ± 0.512
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.054 ± 0.232
5.357 ± 0.306
3.415 ± 0.375
4.866 ± 0.305
5.045 ± 0.384
5.603 ± 0.368
5.982 ± 0.392
5.201 ± 0.302
1.071 ± 0.2
4.531 ± 0.326
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here