Fenneropenaeus chinensis hepatopancreatic densovirus (isolate Shrimp/China/HB/2008)
Average proteome isoelectric point is 7.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E2E2D1|E2E2D1_FCDNV Truncated structural protein OS=Fenneropenaeus chinensis hepatopancreatic densovirus (isolate Shrimp/China/HB/2008) OX=1556324 GN=CP PE=4 SV=1
MM1 pKa = 7.54 SPTRR5 pKa = 11.84 KK6 pKa = 9.67 GGNYY10 pKa = 8.53 FASKK14 pKa = 10.31 HH15 pKa = 4.7 FQSKK19 pKa = 10.11 KK20 pKa = 9.13 KK21 pKa = 10.55 NKK23 pKa = 9.3 LGKK26 pKa = 10.6 VKK28 pKa = 10.68 DD29 pKa = 3.75 LLASKK34 pKa = 10.43 KK35 pKa = 10.17 KK36 pKa = 9.24 EE37 pKa = 3.79 RR38 pKa = 11.84 KK39 pKa = 9.33 FKK41 pKa = 11.23 GKK43 pKa = 10.76 GNTLSEE49 pKa = 4.43 EE50 pKa = 4.36 PSTSGWKK57 pKa = 10.55 DD58 pKa = 3.24 PVRR61 pKa = 11.84 QRR63 pKa = 11.84 FPALEE68 pKa = 3.86 QEE70 pKa = 4.41 EE71 pKa = 4.88 RR72 pKa = 11.84 NTFAGLLAIEE82 pKa = 4.84 AAPDD86 pKa = 3.36 QRR88 pKa = 11.84 QLGRR92 pKa = 11.84 DD93 pKa = 3.64 SNNQLALVQRR103 pKa = 11.84 DD104 pKa = 3.21 TRR106 pKa = 11.84 VAVRR110 pKa = 11.84 QSTNRR115 pKa = 11.84 RR116 pKa = 11.84 EE117 pKa = 3.98 ALEE120 pKa = 4.16 VVRR123 pKa = 11.84 AATQAIRR130 pKa = 11.84 SGGDD134 pKa = 2.78 RR135 pKa = 11.84 LAEE138 pKa = 3.91 LVQTYY143 pKa = 11.05 ASGFSDD149 pKa = 3.04 STEE152 pKa = 3.73 IVEE155 pKa = 5.12 LRR157 pKa = 11.84 QEE159 pKa = 4.14 DD160 pKa = 4.44 RR161 pKa = 11.84 IQRR164 pKa = 11.84 DD165 pKa = 2.98 IFQEE169 pKa = 3.97 EE170 pKa = 4.76 GQNLLAIEE178 pKa = 4.33 IALEE182 pKa = 4.68 GPSSVTQQFDD192 pKa = 3.5 QEE194 pKa = 4.07 KK195 pKa = 8.17 TPAVKK200 pKa = 10.14 RR201 pKa = 11.84 ALEE204 pKa = 3.98 LTQEE208 pKa = 4.21 EE209 pKa = 4.48 EE210 pKa = 3.72 QLEE213 pKa = 4.38 RR214 pKa = 11.84 IEE216 pKa = 4.09 NAKK219 pKa = 10.42 KK220 pKa = 10.62 YY221 pKa = 9.14 IEE223 pKa = 4.1 EE224 pKa = 4.37 VIEE227 pKa = 3.86 EE228 pKa = 4.31 TNRR231 pKa = 11.84 EE232 pKa = 3.79 FEE234 pKa = 4.2 DD235 pKa = 3.5 QVRR238 pKa = 11.84 QEE240 pKa = 4.25 TSASAEE246 pKa = 3.95 DD247 pKa = 4.12 TMASAAPTPMEE258 pKa = 3.89 TSEE261 pKa = 4.57 PGVTAAPHH269 pKa = 5.26 QKK271 pKa = 9.87 SAAGGGGGGGSGGEE285 pKa = 3.79 TAGYY289 pKa = 9.99 GKK291 pKa = 8.49 NTNDD295 pKa = 2.63 AFQRR299 pKa = 11.84 HH300 pKa = 5.5 RR301 pKa = 11.84 NRR303 pKa = 11.84 PIDD306 pKa = 3.9 LKK308 pKa = 11.21 HH309 pKa = 7.3 IGDD312 pKa = 4.01 NVYY315 pKa = 10.11 VAQRR319 pKa = 11.84 VYY321 pKa = 10.73 KK322 pKa = 10.8 VEE324 pKa = 4.25 AEE326 pKa = 4.33 CKK328 pKa = 10.71 NIDD331 pKa = 5.09 EE332 pKa = 4.64 KK333 pKa = 11.16 LTWSNTANNEE343 pKa = 3.75 YY344 pKa = 9.68 TRR346 pKa = 11.84 RR347 pKa = 11.84 LMGTNGNSDD356 pKa = 3.27 KK357 pKa = 11.66 GNVKK361 pKa = 10.37 FNFTAPINGSIGMGNLALGNYY382 pKa = 8.01 INAWGIDD389 pKa = 3.87 NIAKK393 pKa = 10.25 SEE395 pKa = 4.13 DD396 pKa = 2.99 SWAIIATRR404 pKa = 11.84 GKK406 pKa = 9.59 MNHH409 pKa = 5.66 LQAFEE414 pKa = 4.58 MIPPNARR421 pKa = 11.84 RR422 pKa = 11.84 NCSRR426 pKa = 11.84 VYY428 pKa = 10.3 KK429 pKa = 10.44 CSSTVWW435 pKa = 3.08
Molecular weight: 48.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.961
IPC2_protein 7.395
IPC_protein 7.454
Toseland 7.395
ProMoST 7.863
Dawson 8.185
Bjellqvist 8.229
Wikipedia 8.141
Rodwell 8.2
Grimsley 7.38
Solomon 8.361
Lehninger 8.361
Nozaki 8.434
DTASelect 8.17
Thurlkill 8.258
EMBOSS 8.419
Sillero 8.492
Patrickios 4.902
IPC_peptide 8.346
IPC2_peptide 7.161
IPC2.peptide.svr19 7.559
Protein with the highest isoelectric point:
>tr|E2E2D1|E2E2D1_FCDNV Truncated structural protein OS=Fenneropenaeus chinensis hepatopancreatic densovirus (isolate Shrimp/China/HB/2008) OX=1556324 GN=CP PE=4 SV=1
MM1 pKa = 7.14 FRR3 pKa = 11.84 QVMKK7 pKa = 10.6 LKK9 pKa = 10.53 RR10 pKa = 11.84 EE11 pKa = 3.99 GMVDD15 pKa = 3.56 HH16 pKa = 6.85 NPLVTFYY23 pKa = 11.15 SGIIVKK29 pKa = 10.17 FEE31 pKa = 3.69 HH32 pKa = 6.45 WNNNVSKK39 pKa = 10.65 VRR41 pKa = 11.84 KK42 pKa = 8.9 FLYY45 pKa = 10.37 KK46 pKa = 10.25 FAQWLYY52 pKa = 10.68 KK53 pKa = 9.93 EE54 pKa = 4.31 CTYY57 pKa = 10.79 IHH59 pKa = 6.67 NISAAVHH66 pKa = 6.28 DD67 pKa = 4.36 RR68 pKa = 11.84 CKK70 pKa = 10.97 DD71 pKa = 3.59 DD72 pKa = 3.71 CCKK75 pKa = 11.01 DD76 pKa = 3.48 SANKK80 pKa = 9.09 VCKK83 pKa = 10.34 NIYY86 pKa = 9.71 GPHH89 pKa = 5.59 LHH91 pKa = 6.93 ILLEE95 pKa = 4.46 SVNEE99 pKa = 4.01 NWSKK103 pKa = 10.24 SSKK106 pKa = 9.43 RR107 pKa = 11.84 VLFRR111 pKa = 11.84 GYY113 pKa = 10.66 EE114 pKa = 3.98 KK115 pKa = 10.38 ILQHH119 pKa = 6.0 EE120 pKa = 4.48 NKK122 pKa = 9.96 QLWEE126 pKa = 4.18 DD127 pKa = 3.62 LGLQKK132 pKa = 9.75 TSPPSMSLWDD142 pKa = 3.46 GEE144 pKa = 4.3 MFKK147 pKa = 10.43 WYY149 pKa = 9.05 MFRR152 pKa = 11.84 DD153 pKa = 3.22 RR154 pKa = 11.84 KK155 pKa = 8.23 YY156 pKa = 9.98 AKK158 pKa = 10.04 VHH160 pKa = 4.11 GTYY163 pKa = 10.15 YY164 pKa = 10.34 YY165 pKa = 11.0 SSNEE169 pKa = 3.77 EE170 pKa = 4.16 LLSKK174 pKa = 9.86 LVKK177 pKa = 10.36 MKK179 pKa = 9.44 DD180 pKa = 3.14 TQEE183 pKa = 4.66 RR184 pKa = 11.84 DD185 pKa = 3.3 DD186 pKa = 5.25 LYY188 pKa = 11.59 EE189 pKa = 4.19 KK190 pKa = 10.82 ACQFKK195 pKa = 10.39 KK196 pKa = 10.78 DD197 pKa = 3.26 KK198 pKa = 10.64 NAARR202 pKa = 11.84 KK203 pKa = 9.47 IEE205 pKa = 4.08 NSTAKK210 pKa = 9.69 TLDD213 pKa = 3.61 GADD216 pKa = 3.48 SDD218 pKa = 4.58 NIRR221 pKa = 11.84 LSSSRR226 pKa = 11.84 AIYY229 pKa = 10.52 LEE231 pKa = 3.77 NLQVLEE237 pKa = 4.51 KK238 pKa = 10.97 YY239 pKa = 9.74 LVKK242 pKa = 10.5 HH243 pKa = 5.53 KK244 pKa = 10.49 CYY246 pKa = 10.12 TIQDD250 pKa = 3.9 FKK252 pKa = 11.29 MMQRR256 pKa = 11.84 NEE258 pKa = 3.77 DD259 pKa = 4.41 EE260 pKa = 3.64 IWVNYY265 pKa = 9.59 MYY267 pKa = 10.34 DD268 pKa = 3.18 IQNLEE273 pKa = 3.97 KK274 pKa = 10.85 VIEE277 pKa = 4.25 KK278 pKa = 10.44 LNIMEE283 pKa = 4.71 YY284 pKa = 10.69 SLQQADD290 pKa = 4.32 YY291 pKa = 10.9 IEE293 pKa = 4.88 GNTWIGEE300 pKa = 4.27 DD301 pKa = 3.24 LWNTNSAYY309 pKa = 9.59 MRR311 pKa = 11.84 TVKK314 pKa = 10.47 RR315 pKa = 11.84 GTDD318 pKa = 2.6 RR319 pKa = 11.84 YY320 pKa = 8.05 YY321 pKa = 10.05 WYY323 pKa = 9.9 IQRR326 pKa = 11.84 HH327 pKa = 4.17 VSNRR331 pKa = 11.84 ASLIGQSRR339 pKa = 11.84 QICIDD344 pKa = 3.29 GAYY347 pKa = 10.15 MMFNIIDD354 pKa = 3.64 NMRR357 pKa = 11.84 VEE359 pKa = 4.74 SRR361 pKa = 11.84 PKK363 pKa = 9.43 TIPIISKK370 pKa = 9.94 NKK372 pKa = 6.87 TVQWIQDD379 pKa = 3.46 FMDD382 pKa = 5.22 IIHH385 pKa = 6.93 GNLPKK390 pKa = 10.04 INCMMLYY397 pKa = 10.73 GNSNSGKK404 pKa = 7.27 TQLIEE409 pKa = 4.17 ALTGLVNTAIMTNVGDD425 pKa = 3.57 GGTFHH430 pKa = 7.44 FSNITEE436 pKa = 3.89 MSTIVVGNEE445 pKa = 3.64 TKK447 pKa = 10.34 IRR449 pKa = 11.84 TQTIEE454 pKa = 3.66 QWKK457 pKa = 8.9 GLCGGEE463 pKa = 3.99 NVTMPMKK470 pKa = 10.34 YY471 pKa = 9.43 KK472 pKa = 9.73 EE473 pKa = 4.68 HH474 pKa = 5.97 KK475 pKa = 7.9 THH477 pKa = 6.63 MFRR480 pKa = 11.84 KK481 pKa = 9.29 PVFLTNQHH489 pKa = 6.26 HH490 pKa = 6.51 PLVDD494 pKa = 3.13 ISHH497 pKa = 6.45 YY498 pKa = 10.31 DD499 pKa = 3.36 DD500 pKa = 4.04 RR501 pKa = 11.84 RR502 pKa = 11.84 AIEE505 pKa = 4.28 NRR507 pKa = 11.84 SFMYY511 pKa = 10.08 KK512 pKa = 9.92 VEE514 pKa = 4.53 LGSEE518 pKa = 4.2 PVNAHH523 pKa = 5.48 IKK525 pKa = 10.03 FPNRR529 pKa = 11.84 MIPIKK534 pKa = 10.4 KK535 pKa = 9.95 NPEE538 pKa = 3.49 LTQFVLASMQYY549 pKa = 9.65 VHH551 pKa = 6.69 TNYY554 pKa = 9.66 MNRR557 pKa = 11.84 PDD559 pKa = 3.86 KK560 pKa = 10.74 KK561 pKa = 10.69 FKK563 pKa = 10.42 IGFFNKK569 pKa = 10.31 LYY571 pKa = 10.73 DD572 pKa = 3.86 ALFEE576 pKa = 4.33 NSS578 pKa = 3.11
Molecular weight: 68.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.453
IPC2_protein 8.419
IPC_protein 8.258
Toseland 8.829
ProMoST 8.799
Dawson 9.209
Bjellqvist 9.092
Wikipedia 9.37
Rodwell 9.385
Grimsley 9.224
Solomon 9.253
Lehninger 9.209
Nozaki 9.18
DTASelect 8.99
Thurlkill 9.092
EMBOSS 9.341
Sillero 9.268
Patrickios 4.431
IPC_peptide 9.238
IPC2_peptide 7.761
IPC2.peptide.svr19 7.672
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
1440
427
578
480.0
55.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.347 ± 1.66
1.528 ± 0.332
5.139 ± 0.301
7.917 ± 0.914
4.167 ± 0.586
5.903 ± 0.88
2.431 ± 0.53
5.694 ± 0.764
9.028 ± 0.875
6.25 ± 0.425
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.889 ± 0.881
7.083 ± 0.265
2.986 ± 0.212
4.444 ± 0.518
5.556 ± 0.694
6.111 ± 0.234
5.069 ± 0.494
5.556 ± 0.391
1.736 ± 0.234
4.167 ± 0.832
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here