Neolentinus lepideus HHB14362 ss-1
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13108 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A165Q383|A0A165Q383_9AGAM Uncharacterized protein OS=Neolentinus lepideus HHB14362 ss-1 OX=1314782 GN=NEOLEDRAFT_1171731 PE=4 SV=1
MM1 pKa = 7.37 SFCVDD6 pKa = 3.89 PSTTFVDD13 pKa = 3.5 QTLVSDD19 pKa = 3.83 IQAPIFSTHH28 pKa = 5.83 VDD30 pKa = 3.56 CADD33 pKa = 3.48 AGAAPSVHH41 pKa = 6.64 IDD43 pKa = 3.58 LAAFNTSAKK52 pKa = 8.94 VNSMSHH58 pKa = 5.93 LTTNGASEE66 pKa = 4.38 NVSFPAPSLRR76 pKa = 11.84 DD77 pKa = 3.5 EE78 pKa = 4.51 VFLPSTFTGGYY89 pKa = 6.88 WRR91 pKa = 11.84 SHH93 pKa = 4.46 EE94 pKa = 4.28 VGVCVDD100 pKa = 3.19 GTEE103 pKa = 4.7 RR104 pKa = 11.84 SLEE107 pKa = 4.05 DD108 pKa = 3.39 LAAVASLGDD117 pKa = 3.7 QPDD120 pKa = 3.52 VAVFGDD126 pKa = 3.62 ARR128 pKa = 11.84 RR129 pKa = 11.84 NGPMSCSAPVDD140 pKa = 3.34 QLQQLEE146 pKa = 4.24 QDD148 pKa = 4.14 FRR150 pKa = 11.84 AQPAINGCAPLGPDD164 pKa = 4.17 AADD167 pKa = 3.42 ASAPPSTPLLAMKK180 pKa = 10.11 ALPAEE185 pKa = 4.6 DD186 pKa = 4.47 VVDD189 pKa = 3.96 DD190 pKa = 4.68 HH191 pKa = 7.26 PSFVDD196 pKa = 4.13 DD197 pKa = 4.3 SQSSTWLSFEE207 pKa = 4.26 HH208 pKa = 7.22 ADD210 pKa = 4.27 VDD212 pKa = 4.79 LEE214 pKa = 4.6 LPALLDD220 pKa = 3.78 SCDD223 pKa = 3.53 VPFNSPSPSYY233 pKa = 11.01 NIGLFDD239 pKa = 4.64 FDD241 pKa = 4.87 YY242 pKa = 10.82 PPSPSTASATSSFDD256 pKa = 3.61 SPPPTPLLSSLDD268 pKa = 3.79 ISCLEE273 pKa = 4.16 CSQYY277 pKa = 11.37 AQDD280 pKa = 3.43 GHH282 pKa = 6.55 AVCGTCGFTALLGMEE297 pKa = 4.94 GGCGGSGVRR306 pKa = 11.84 VVNAGEE312 pKa = 4.02 DD313 pKa = 3.59 LMDD316 pKa = 3.84 GVEE319 pKa = 4.37 DD320 pKa = 4.77 SRR322 pKa = 11.84 GGCCDD327 pKa = 4.07 VIPGLQHH334 pKa = 5.91 TWTSKK339 pKa = 9.87 IPLDD343 pKa = 3.69 GGYY346 pKa = 10.62 KK347 pKa = 10.4 FGQLPPQAFEE357 pKa = 3.78 QDD359 pKa = 3.47 EE360 pKa = 4.78 VNTLGVDD367 pKa = 4.67 FSAA370 pKa = 5.42
Molecular weight: 38.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.738
IPC2_protein 3.77
IPC_protein 3.808
Toseland 3.567
ProMoST 3.973
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.757
Rodwell 3.63
Grimsley 3.478
Solomon 3.795
Lehninger 3.757
Nozaki 3.91
DTASelect 4.202
Thurlkill 3.63
EMBOSS 3.77
Sillero 3.923
Patrickios 0.617
IPC_peptide 3.795
IPC2_peptide 3.897
IPC2.peptide.svr19 3.807
Protein with the highest isoelectric point:
>tr|A0A165NQK6|A0A165NQK6_9AGAM G-patch domain-containing protein OS=Neolentinus lepideus HHB14362 ss-1 OX=1314782 GN=NEOLEDRAFT_1182897 PE=4 SV=1
MM1 pKa = 7.54 PRR3 pKa = 11.84 ILKK6 pKa = 9.67 QVAQLLARR14 pKa = 11.84 PPALPHH20 pKa = 6.24 ASSVVSTLTGLHH32 pKa = 6.2 RR33 pKa = 11.84 PSSQRR38 pKa = 11.84 LTPLPFALAFQSSSLLTPSFVGRR61 pKa = 11.84 SSALLQLNQVRR72 pKa = 11.84 WGSRR76 pKa = 11.84 GTEE79 pKa = 3.93 YY80 pKa = 10.77 QPSQRR85 pKa = 11.84 VRR87 pKa = 11.84 KK88 pKa = 9.28 RR89 pKa = 11.84 RR90 pKa = 11.84 HH91 pKa = 4.87 GFLARR96 pKa = 11.84 KK97 pKa = 9.53 KK98 pKa = 9.03 SQHH101 pKa = 5.36 GPKK104 pKa = 9.63 ILARR108 pKa = 11.84 RR109 pKa = 11.84 RR110 pKa = 11.84 AKK112 pKa = 9.85 GRR114 pKa = 11.84 KK115 pKa = 8.43 FLSHH119 pKa = 6.98
Molecular weight: 13.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.423
IPC2_protein 11.038
IPC_protein 12.486
Toseland 12.647
ProMoST 13.144
Dawson 12.647
Bjellqvist 12.647
Wikipedia 13.115
Rodwell 12.325
Grimsley 12.691
Solomon 13.144
Lehninger 13.042
Nozaki 12.647
DTASelect 12.647
Thurlkill 12.647
EMBOSS 13.144
Sillero 12.647
Patrickios 12.047
IPC_peptide 13.144
IPC2_peptide 12.135
IPC2.peptide.svr19 9.081
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
13106
2
13108
5153932
49
5057
393.2
43.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.431 ± 0.019
1.331 ± 0.009
5.557 ± 0.013
6.016 ± 0.021
3.597 ± 0.013
6.504 ± 0.021
2.525 ± 0.01
4.819 ± 0.016
4.535 ± 0.02
9.236 ± 0.027
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.181 ± 0.008
3.301 ± 0.01
6.398 ± 0.025
3.681 ± 0.014
6.362 ± 0.021
8.743 ± 0.03
5.935 ± 0.015
6.554 ± 0.016
1.496 ± 0.009
2.799 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here