Streptococcus satellite phage Javan2

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 7.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZGH5|A0A4D5ZGH5_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan2 OX=2558562 GN=JavanS2_0010 PE=4 SV=1
MM1 pKa = 8.16DD2 pKa = 5.21KK3 pKa = 11.35GKK5 pKa = 10.23ILDD8 pKa = 3.83NYY10 pKa = 10.37EE11 pKa = 4.43DD12 pKa = 4.05LTSGMMIDD20 pKa = 3.94YY21 pKa = 10.75DD22 pKa = 3.99DD23 pKa = 4.94VKK25 pKa = 11.23AQLIDD30 pKa = 3.89LVTVSDD36 pKa = 4.02MALSLDD42 pKa = 4.37DD43 pKa = 5.05RR44 pKa = 11.84NSSYY48 pKa = 10.82ATLNIMNMAIQQLVDD63 pKa = 3.33KK64 pKa = 11.31LEE66 pKa = 4.04EE67 pKa = 4.5RR68 pKa = 11.84DD69 pKa = 3.26NHH71 pKa = 5.92ARR73 pKa = 3.51

Molecular weight: 8.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZKG0|A0A4D5ZKG0_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan2 OX=2558562 GN=JavanS2_0002 PE=4 SV=1
MM1 pKa = 7.34GGKK4 pKa = 8.88HH5 pKa = 6.12LKK7 pKa = 10.38HH8 pKa = 6.14PALRR12 pKa = 11.84GLRR15 pKa = 11.84IGRR18 pKa = 11.84EE19 pKa = 4.19SNVKK23 pKa = 10.05DD24 pKa = 3.36RR25 pKa = 11.84QLTFEE30 pKa = 4.46QIKK33 pKa = 9.24EE34 pKa = 3.91QQRR37 pKa = 11.84LKK39 pKa = 10.73KK40 pKa = 10.24LKK42 pKa = 9.7KK43 pKa = 9.62KK44 pKa = 9.77RR45 pKa = 11.84RR46 pKa = 11.84KK47 pKa = 9.82HH48 pKa = 4.41EE49 pKa = 3.86

Molecular weight: 5.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15

0

15

2087

40

412

139.1

16.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.414 ± 0.59

0.575 ± 0.146

4.792 ± 0.577

8.529 ± 0.744

3.306 ± 0.448

5.319 ± 0.595

2.108 ± 0.29

7.475 ± 0.587

8.529 ± 0.805

11.404 ± 0.763

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.108 ± 0.393

4.935 ± 0.351

2.923 ± 0.257

4.839 ± 0.446

5.846 ± 0.596

5.798 ± 0.562

6.133 ± 0.469

4.456 ± 0.548

0.91 ± 0.191

4.6 ± 0.366
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski