Streptococcus phage SM1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q7Y4K0|Q7Y4K0_9CAUD Gp24 OS=Streptococcus phage SM1 OX=157924 GN=24 PE=4 SV=1
MM1 pKa = 7.67IIKK4 pKa = 9.93DD5 pKa = 3.68YY6 pKa = 10.92KK7 pKa = 10.69YY8 pKa = 10.29DD9 pKa = 3.75FSSGRR14 pKa = 11.84IRR16 pKa = 11.84YY17 pKa = 9.44IIDD20 pKa = 3.67ADD22 pKa = 3.69GHH24 pKa = 5.34EE25 pKa = 4.52LAMEE29 pKa = 4.2HH30 pKa = 6.28TKK32 pKa = 10.06TEE34 pKa = 4.18YY35 pKa = 11.21GSVQRR40 pKa = 11.84DD41 pKa = 3.92DD42 pKa = 3.96IDD44 pKa = 3.85VFLLAVEE51 pKa = 5.0EE52 pKa = 4.49YY53 pKa = 10.65DD54 pKa = 3.64FQEE57 pKa = 5.91AEE59 pKa = 4.15MIEE62 pKa = 4.27AFVDD66 pKa = 4.31FQNDD70 pKa = 3.31LLLYY74 pKa = 10.76GIDD77 pKa = 3.51FEE79 pKa = 4.85LRR81 pKa = 11.84KK82 pKa = 9.64EE83 pKa = 4.14ATDD86 pKa = 3.28

Molecular weight: 10.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q7Y4M3|Q7Y4M3_9CAUD Integrase OS=Streptococcus phage SM1 OX=157924 GN=1 PE=3 SV=1
MM1 pKa = 7.66KK2 pKa = 10.08IGMRR6 pKa = 11.84TPSLKK11 pKa = 10.35KK12 pKa = 9.79SLKK15 pKa = 10.57ARR17 pKa = 11.84TTSKK21 pKa = 9.99WKK23 pKa = 10.15RR24 pKa = 11.84QAKK27 pKa = 9.07KK28 pKa = 10.57AIVPGYY34 pKa = 9.14GKK36 pKa = 10.56KK37 pKa = 10.34GVGWIKK43 pKa = 10.52NPKK46 pKa = 8.09KK47 pKa = 10.66AMYY50 pKa = 10.0NKK52 pKa = 9.42VYY54 pKa = 10.65HH55 pKa = 5.99KK56 pKa = 8.14TTFGLSDD63 pKa = 3.86LLKK66 pKa = 10.09TSKK69 pKa = 10.21KK70 pKa = 9.39RR71 pKa = 11.84NKK73 pKa = 9.24RR74 pKa = 11.84TTKK77 pKa = 9.74SQTSIKK83 pKa = 8.95STKK86 pKa = 10.04KK87 pKa = 10.74YY88 pKa = 9.62GAKK91 pKa = 9.89DD92 pKa = 3.51YY93 pKa = 11.16KK94 pKa = 10.57EE95 pKa = 4.12AGIVLIVIGAVLVFLLPPISFIFITTGFISFIIGHH130 pKa = 6.62FSMKK134 pKa = 10.52CEE136 pKa = 3.51IKK138 pKa = 10.42KK139 pKa = 10.19RR140 pKa = 11.84QNQKK144 pKa = 10.91DD145 pKa = 3.57VV146 pKa = 3.19

Molecular weight: 16.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

10599

42

1062

189.3

21.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.614 ± 0.496

0.859 ± 0.143

6.18 ± 0.306

7.425 ± 0.346

4.151 ± 0.241

6.538 ± 0.449

1.793 ± 0.206

7.491 ± 0.321

8.586 ± 0.45

8.312 ± 0.269

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.481 ± 0.157

5.434 ± 0.207

2.83 ± 0.269

3.953 ± 0.259

4.378 ± 0.35

6.274 ± 0.277

5.689 ± 0.279

5.689 ± 0.188

1.425 ± 0.194

3.897 ± 0.324
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski