Streptococcus phage SM1
Average proteome isoelectric point is 6.23
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q7Y4K0|Q7Y4K0_9CAUD Gp24 OS=Streptococcus phage SM1 OX=157924 GN=24 PE=4 SV=1
MM1 pKa = 7.67 IIKK4 pKa = 9.93 DD5 pKa = 3.68 YY6 pKa = 10.92 KK7 pKa = 10.69 YY8 pKa = 10.29 DD9 pKa = 3.75 FSSGRR14 pKa = 11.84 IRR16 pKa = 11.84 YY17 pKa = 9.44 IIDD20 pKa = 3.67 ADD22 pKa = 3.69 GHH24 pKa = 5.34 EE25 pKa = 4.52 LAMEE29 pKa = 4.2 HH30 pKa = 6.28 TKK32 pKa = 10.06 TEE34 pKa = 4.18 YY35 pKa = 11.21 GSVQRR40 pKa = 11.84 DD41 pKa = 3.92 DD42 pKa = 3.96 IDD44 pKa = 3.85 VFLLAVEE51 pKa = 5.0 EE52 pKa = 4.49 YY53 pKa = 10.65 DD54 pKa = 3.64 FQEE57 pKa = 5.91 AEE59 pKa = 4.15 MIEE62 pKa = 4.27 AFVDD66 pKa = 4.31 FQNDD70 pKa = 3.31 LLLYY74 pKa = 10.76 GIDD77 pKa = 3.51 FEE79 pKa = 4.85 LRR81 pKa = 11.84 KK82 pKa = 9.64 EE83 pKa = 4.14 ATDD86 pKa = 3.28
Molecular weight: 10.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.17
IPC2_protein 4.088
IPC_protein 4.024
Toseland 3.834
ProMoST 4.113
Dawson 4.012
Bjellqvist 4.202
Wikipedia 3.923
Rodwell 3.859
Grimsley 3.745
Solomon 3.999
Lehninger 3.948
Nozaki 4.126
DTASelect 4.329
Thurlkill 3.872
EMBOSS 3.935
Sillero 4.151
Patrickios 3.376
IPC_peptide 3.999
IPC2_peptide 4.126
IPC2.peptide.svr19 4.071
Protein with the highest isoelectric point:
>tr|Q7Y4M3|Q7Y4M3_9CAUD Integrase OS=Streptococcus phage SM1 OX=157924 GN=1 PE=3 SV=1
MM1 pKa = 7.66 KK2 pKa = 10.08 IGMRR6 pKa = 11.84 TPSLKK11 pKa = 10.35 KK12 pKa = 9.79 SLKK15 pKa = 10.57 ARR17 pKa = 11.84 TTSKK21 pKa = 9.99 WKK23 pKa = 10.15 RR24 pKa = 11.84 QAKK27 pKa = 9.07 KK28 pKa = 10.57 AIVPGYY34 pKa = 9.14 GKK36 pKa = 10.56 KK37 pKa = 10.34 GVGWIKK43 pKa = 10.52 NPKK46 pKa = 8.09 KK47 pKa = 10.66 AMYY50 pKa = 10.0 NKK52 pKa = 9.42 VYY54 pKa = 10.65 HH55 pKa = 5.99 KK56 pKa = 8.14 TTFGLSDD63 pKa = 3.86 LLKK66 pKa = 10.09 TSKK69 pKa = 10.21 KK70 pKa = 9.39 RR71 pKa = 11.84 NKK73 pKa = 9.24 RR74 pKa = 11.84 TTKK77 pKa = 9.74 SQTSIKK83 pKa = 8.95 STKK86 pKa = 10.04 KK87 pKa = 10.74 YY88 pKa = 9.62 GAKK91 pKa = 9.89 DD92 pKa = 3.51 YY93 pKa = 11.16 KK94 pKa = 10.57 EE95 pKa = 4.12 AGIVLIVIGAVLVFLLPPISFIFITTGFISFIIGHH130 pKa = 6.62 FSMKK134 pKa = 10.52 CEE136 pKa = 3.51 IKK138 pKa = 10.42 KK139 pKa = 10.19 RR140 pKa = 11.84 QNQKK144 pKa = 10.91 DD145 pKa = 3.57 VV146 pKa = 3.19
Molecular weight: 16.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.194
IPC2_protein 10.087
IPC_protein 10.189
Toseland 11.008
ProMoST 10.467
Dawson 11.067
Bjellqvist 10.643
Wikipedia 11.169
Rodwell 11.784
Grimsley 11.096
Solomon 11.111
Lehninger 11.096
Nozaki 10.965
DTASelect 10.643
Thurlkill 10.965
EMBOSS 11.374
Sillero 10.979
Patrickios 11.506
IPC_peptide 11.125
IPC2_peptide 9.136
IPC2.peptide.svr19 8.481
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
56
0
56
10599
42
1062
189.3
21.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.614 ± 0.496
0.859 ± 0.143
6.18 ± 0.306
7.425 ± 0.346
4.151 ± 0.241
6.538 ± 0.449
1.793 ± 0.206
7.491 ± 0.321
8.586 ± 0.45
8.312 ± 0.269
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.481 ± 0.157
5.434 ± 0.207
2.83 ± 0.269
3.953 ± 0.259
4.378 ± 0.35
6.274 ± 0.277
5.689 ± 0.279
5.689 ± 0.188
1.425 ± 0.194
3.897 ± 0.324
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here