Infectious hypodermal and hematopoietic necrosis virus (IHHNV) (Decapod penstyldensovirus 1)
Average proteome isoelectric point is 6.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9IBP6|Q9IBP6_IHHNV Non-structural protein OS=Infectious hypodermal and hematopoietic necrosis virus OX=1513224 PE=4 SV=1
MM1 pKa = 7.61 AKK3 pKa = 10.33 DD4 pKa = 3.34 ILHH7 pKa = 5.97 TRR9 pKa = 11.84 QGEE12 pKa = 4.22 PEE14 pKa = 4.3 SLSEE18 pKa = 4.48 LLRR21 pKa = 11.84 EE22 pKa = 4.39 RR23 pKa = 11.84 TNEE26 pKa = 3.91 THH28 pKa = 6.69 SSQGISPSLLTPGPNQDD45 pKa = 3.87 PKK47 pKa = 10.47 PTTEE51 pKa = 3.69 QLLNMSEE58 pKa = 4.49 EE59 pKa = 4.32 LFQFSDD65 pKa = 4.19 EE66 pKa = 4.49 EE67 pKa = 4.99 DD68 pKa = 3.55 NSQTPPRR75 pKa = 11.84 TSTPEE80 pKa = 3.48 QTDD83 pKa = 3.3 PKK85 pKa = 10.77 VCVDD89 pKa = 3.31 NLGIRR94 pKa = 11.84 EE95 pKa = 4.31 GTGNGTIQLGSEE107 pKa = 4.5 SEE109 pKa = 4.25 TSLGSVGNSNDD120 pKa = 3.5 RR121 pKa = 11.84 GKK123 pKa = 10.64 KK124 pKa = 6.8 RR125 pKa = 11.84 QRR127 pKa = 11.84 GITYY131 pKa = 10.1 ISDD134 pKa = 3.57 TSDD137 pKa = 2.95 SSGSDD142 pKa = 3.58 DD143 pKa = 4.09 EE144 pKa = 5.38 NLGTPHH150 pKa = 6.87 RR151 pKa = 11.84 NKK153 pKa = 9.66 RR154 pKa = 11.84 TRR156 pKa = 11.84 NSNTTKK162 pKa = 9.75 EE163 pKa = 4.33 TGGGDD168 pKa = 3.37 NRR170 pKa = 11.84 RR171 pKa = 11.84 HH172 pKa = 5.48 QEE174 pKa = 3.68 SDD176 pKa = 2.77 HH177 pKa = 6.22 GSNGNRR183 pKa = 11.84 LEE185 pKa = 4.2 PTDD188 pKa = 4.16 GGEE191 pKa = 4.31 SCSSGTQPDD200 pKa = 5.02 FIEE203 pKa = 4.72 GTPNGPDD210 pKa = 3.15 EE211 pKa = 4.25 MDD213 pKa = 3.0 GRR215 pKa = 11.84 RR216 pKa = 11.84 LEE218 pKa = 3.88 EE219 pKa = 4.15 SEE221 pKa = 3.86 IDD223 pKa = 3.57 KK224 pKa = 10.85 QVEE227 pKa = 4.15 STTWYY232 pKa = 9.04 TFVIRR237 pKa = 11.84 EE238 pKa = 4.04 KK239 pKa = 9.67 PQPRR243 pKa = 11.84 RR244 pKa = 11.84 LSGRR248 pKa = 11.84 TPNFTITDD256 pKa = 4.0 HH257 pKa = 6.73 GDD259 pKa = 2.86 HH260 pKa = 5.68 WHH262 pKa = 6.06 ITYY265 pKa = 10.5 SGHH268 pKa = 5.99 PTNKK272 pKa = 7.59 TRR274 pKa = 11.84 HH275 pKa = 5.82 RR276 pKa = 11.84 ATILAYY282 pKa = 10.75 LGVTFAARR290 pKa = 11.84 AEE292 pKa = 4.22 AEE294 pKa = 4.06 ATTVLVRR301 pKa = 11.84 NIKK304 pKa = 10.03 RR305 pKa = 11.84 WILYY309 pKa = 8.45 LIRR312 pKa = 11.84 YY313 pKa = 7.88 GIEE316 pKa = 3.49 RR317 pKa = 11.84 LSYY320 pKa = 10.4 FGLGHH325 pKa = 7.53 AIFKK329 pKa = 10.55 RR330 pKa = 11.84 IIKK333 pKa = 9.25 YY334 pKa = 7.27 FQQYY338 pKa = 9.86 RR339 pKa = 11.84 RR340 pKa = 11.84 DD341 pKa = 3.56 EE342 pKa = 4.39 DD343 pKa = 4.77 AVDD346 pKa = 4.96 GPCPYY351 pKa = 8.15 MTTTRR356 pKa = 11.84 EE357 pKa = 3.77 DD358 pKa = 3.41 RR359 pKa = 11.84 AEE361 pKa = 4.17 EE362 pKa = 4.12 KK363 pKa = 10.47 PKK365 pKa = 10.81 EE366 pKa = 3.92 NSAEE370 pKa = 3.88 YY371 pKa = 10.14 DD372 pKa = 3.78 YY373 pKa = 11.29 LQHH376 pKa = 6.98 LVKK379 pKa = 9.86 TKK381 pKa = 9.44 SARR384 pKa = 11.84 TVQEE388 pKa = 4.28 LVNKK392 pKa = 10.41 LDD394 pKa = 3.88 DD395 pKa = 4.09 EE396 pKa = 4.77 EE397 pKa = 5.64 YY398 pKa = 9.93 KK399 pKa = 10.68 QLWTRR404 pKa = 11.84 TRR406 pKa = 11.84 GQYY409 pKa = 9.99 KK410 pKa = 10.45 DD411 pKa = 3.35 KK412 pKa = 11.13 LRR414 pKa = 11.84 GILTYY419 pKa = 11.05 YY420 pKa = 10.42 NNKK423 pKa = 9.52 KK424 pKa = 10.26 KK425 pKa = 10.87 SNQSQLSLITNLQNISKK442 pKa = 10.41 RR443 pKa = 11.84 KK444 pKa = 8.29 PDD446 pKa = 3.96 YY447 pKa = 11.73 DD448 pKa = 3.19 NMQWIKK454 pKa = 11.46 YY455 pKa = 7.86 MLANNDD461 pKa = 2.63 IRR463 pKa = 11.84 VPEE466 pKa = 3.87 ILAWIIIVADD476 pKa = 3.85 KK477 pKa = 11.04 KK478 pKa = 10.74 LDD480 pKa = 4.15 KK481 pKa = 11.06 INTLVLQGPTGTGKK495 pKa = 10.59 SLTIGALLGKK505 pKa = 10.23 LNTGLVTRR513 pKa = 11.84 TGDD516 pKa = 3.32 SNTFHH521 pKa = 6.75 LQNLIGKK528 pKa = 9.11 SYY530 pKa = 11.56 ALFEE534 pKa = 4.41 EE535 pKa = 4.68 PRR537 pKa = 11.84 ISQITVDD544 pKa = 4.01 DD545 pKa = 4.36 FKK547 pKa = 11.82 LLFEE551 pKa = 4.99 GSDD554 pKa = 3.38 LEE556 pKa = 4.9 VNIKK560 pKa = 9.77 HH561 pKa = 5.57 QEE563 pKa = 3.88 SEE565 pKa = 3.78 IMGRR569 pKa = 11.84 IPIFISTNKK578 pKa = 10.59 DD579 pKa = 2.13 IDD581 pKa = 3.87 YY582 pKa = 8.44 WVPPADD588 pKa = 3.56 GKK590 pKa = 10.96 ALQTRR595 pKa = 11.84 TKK597 pKa = 9.22 TFHH600 pKa = 6.65 LTRR603 pKa = 11.84 QIKK606 pKa = 10.09 GLSDD610 pKa = 3.52 RR611 pKa = 11.84 MNSQYY616 pKa = 11.04 DD617 pKa = 3.63 INPPPDD623 pKa = 5.19 KK624 pKa = 10.18 ITSDD628 pKa = 3.66 DD629 pKa = 3.76 FLGLFQEE636 pKa = 4.62 YY637 pKa = 9.77 EE638 pKa = 4.23 KK639 pKa = 10.94 EE640 pKa = 3.65 IDD642 pKa = 4.68 DD643 pKa = 5.48 IIDD646 pKa = 3.14 NHH648 pKa = 5.65 VRR650 pKa = 11.84 RR651 pKa = 11.84 FNKK654 pKa = 10.1 SKK656 pKa = 10.22 PKK658 pKa = 10.48 EE659 pKa = 4.2 KK660 pKa = 10.03 IQEE663 pKa = 4.24 GCTT666 pKa = 3.34
Molecular weight: 75.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.975
IPC2_protein 5.881
IPC_protein 5.944
Toseland 6.376
ProMoST 6.275
Dawson 6.198
Bjellqvist 6.211
Wikipedia 6.211
Rodwell 6.186
Grimsley 6.561
Solomon 6.198
Lehninger 6.198
Nozaki 6.465
DTASelect 6.649
Thurlkill 6.664
EMBOSS 6.634
Sillero 6.561
Patrickios 4.507
IPC_peptide 6.211
IPC2_peptide 6.504
IPC2.peptide.svr19 6.551
Protein with the highest isoelectric point:
>tr|Q9IBP6|Q9IBP6_IHHNV Non-structural protein OS=Infectious hypodermal and hematopoietic necrosis virus OX=1513224 PE=4 SV=1
MM1 pKa = 7.45 CADD4 pKa = 3.67 STRR7 pKa = 11.84 ASPRR11 pKa = 11.84 KK12 pKa = 9.04 RR13 pKa = 11.84 SRR15 pKa = 11.84 RR16 pKa = 11.84 DD17 pKa = 2.94 AHH19 pKa = 7.48 NEE21 pKa = 3.86 DD22 pKa = 4.02 EE23 pKa = 4.32 EE24 pKa = 4.36 HH25 pKa = 7.23 AEE27 pKa = 4.38 GSSGPDD33 pKa = 3.04 PHH35 pKa = 7.45 RR36 pKa = 11.84 CLQFNTGDD44 pKa = 3.93 SIHH47 pKa = 6.5 ITFQTRR53 pKa = 11.84 RR54 pKa = 11.84 YY55 pKa = 10.23 FEE57 pKa = 4.23 FDD59 pKa = 2.97 AANDD63 pKa = 3.83 GNFDD67 pKa = 4.09 GKK69 pKa = 10.31 NLYY72 pKa = 9.9 CLPLHH77 pKa = 6.52 WMNLYY82 pKa = 10.75 LYY84 pKa = 9.21 GLKK87 pKa = 10.61 SSDD90 pKa = 3.18 SSATEE95 pKa = 3.65 TQRR98 pKa = 11.84 YY99 pKa = 9.58 KK100 pKa = 10.15 MVKK103 pKa = 10.47 SMMKK107 pKa = 7.99 TYY109 pKa = 9.6 GWKK112 pKa = 8.21 VHH114 pKa = 5.67 KK115 pKa = 10.34 AGVVMHH121 pKa = 6.44 SMVPLMKK128 pKa = 10.15 DD129 pKa = 3.46 LKK131 pKa = 10.83 VSGGTSFEE139 pKa = 4.36 TLTFTDD145 pKa = 3.65 TPYY148 pKa = 11.48 LEE150 pKa = 4.37 IFKK153 pKa = 9.51 DD154 pKa = 3.47 TTGLHH159 pKa = 5.71 NQLSTKK165 pKa = 10.01 EE166 pKa = 3.94 ADD168 pKa = 3.15 VTLAKK173 pKa = 9.98 WIQNPQLVTVQSTAANYY190 pKa = 8.02 EE191 pKa = 4.03 DD192 pKa = 6.0 PIQQFGFMEE201 pKa = 3.91 QMRR204 pKa = 11.84 TGDD207 pKa = 3.1 RR208 pKa = 11.84 KK209 pKa = 10.66 AYY211 pKa = 8.23 TIHH214 pKa = 7.06 GDD216 pKa = 3.05 TRR218 pKa = 11.84 NWYY221 pKa = 9.88 GGEE224 pKa = 4.04 IPTTGPTFIPKK235 pKa = 9.32 WGGQLKK241 pKa = 9.31 WDD243 pKa = 4.17 KK244 pKa = 10.63 PSLGNLVYY252 pKa = 10.1 PADD255 pKa = 3.48 HH256 pKa = 6.75 HH257 pKa = 6.6 TNDD260 pKa = 2.99 WQQIFMRR267 pKa = 11.84 MSPIKK272 pKa = 10.47 GPNGDD277 pKa = 4.13 EE278 pKa = 4.3 LKK280 pKa = 10.86 LGCRR284 pKa = 11.84 VQADD288 pKa = 4.32 FFLHH292 pKa = 6.57 LEE294 pKa = 4.12 VRR296 pKa = 11.84 LPPQGCVASLGMLQYY311 pKa = 11.12 LHH313 pKa = 7.0 APCTGQLNKK322 pKa = 10.32 CYY324 pKa = 10.26 IMHH327 pKa = 6.7 TNN329 pKa = 3.14
Molecular weight: 37.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.602
IPC2_protein 6.634
IPC_protein 6.766
Toseland 6.839
ProMoST 7.176
Dawson 7.278
Bjellqvist 7.22
Wikipedia 7.249
Rodwell 7.263
Grimsley 6.956
Solomon 7.307
Lehninger 7.322
Nozaki 7.556
DTASelect 7.571
Thurlkill 7.614
EMBOSS 7.629
Sillero 7.702
Patrickios 4.101
IPC_peptide 7.307
IPC2_peptide 7.176
IPC2.peptide.svr19 7.018
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
1358
329
666
452.7
51.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.535 ± 0.581
1.105 ± 0.411
6.112 ± 0.842
7.216 ± 1.054
3.019 ± 0.43
6.112 ± 1.332
2.946 ± 0.493
5.817 ± 0.95
6.48 ± 0.197
8.689 ± 0.523
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.135 ± 0.814
5.67 ± 0.386
5.744 ± 0.622
5.302 ± 0.385
6.038 ± 0.652
6.48 ± 0.485
9.352 ± 0.507
3.756 ± 0.521
1.694 ± 0.427
2.798 ± 0.675
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here