Ovine gammaherpesvirus 2
Average proteome isoelectric point is 6.5
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 74 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q2VSJ2|Q2VSJ2_9GAMA ORF49 OS=Ovine gammaherpesvirus 2 OX=10398 GN=OvHV-2gp47 PE=4 SV=1
MM1 pKa = 7.86 RR2 pKa = 11.84 FPHH5 pKa = 7.06 KK6 pKa = 10.6 LLLLEE11 pKa = 4.23 ILCVLAALNICITSFTLGCFALYY34 pKa = 9.82 RR35 pKa = 11.84 QLVHH39 pKa = 7.97 ISIGNLSFPHH49 pKa = 6.92 RR50 pKa = 11.84 DD51 pKa = 2.8 GDD53 pKa = 3.94 EE54 pKa = 4.34 VVRR57 pKa = 11.84 LMYY60 pKa = 10.21 IPPLNDD66 pKa = 2.93 SVEE69 pKa = 4.14 FSYY72 pKa = 10.96 PPTVFPTEE80 pKa = 4.37 EE81 pKa = 3.99 EE82 pKa = 4.07 DD83 pKa = 4.37 LDD85 pKa = 4.13 YY86 pKa = 11.52 DD87 pKa = 4.26 SDD89 pKa = 3.73 EE90 pKa = 4.45 LLYY93 pKa = 10.68 EE94 pKa = 4.79 DD95 pKa = 5.49 EE96 pKa = 4.81 YY97 pKa = 11.66 SDD99 pKa = 3.57 PTVEE103 pKa = 4.12 PWLDD107 pKa = 3.48 YY108 pKa = 11.25 YY109 pKa = 11.15 DD110 pKa = 4.44 YY111 pKa = 11.01 EE112 pKa = 6.05 SEE114 pKa = 4.26 DD115 pKa = 2.8 SWYY118 pKa = 10.81 YY119 pKa = 11.27 DD120 pKa = 3.13 SS121 pKa = 5.61
Molecular weight: 14.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.884
IPC2_protein 3.808
IPC_protein 3.783
Toseland 3.579
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.694
Rodwell 3.605
Grimsley 3.49
Solomon 3.745
Lehninger 3.706
Nozaki 3.884
DTASelect 4.075
Thurlkill 3.63
EMBOSS 3.694
Sillero 3.897
Patrickios 0.057
IPC_peptide 3.745
IPC2_peptide 3.872
IPC2.peptide.svr19 3.816
Protein with the highest isoelectric point:
>tr|Q2VSN4|Q2VSN4_9GAMA Ov3.5 OS=Ovine gammaherpesvirus 2 OX=10398 GN=Ov3.5 PE=4 SV=1
MM1 pKa = 6.91 FQSILSQRR9 pKa = 11.84 PICALSSLPAKK20 pKa = 10.27 RR21 pKa = 11.84 KK22 pKa = 9.09 VAGLNAHH29 pKa = 6.28 RR30 pKa = 11.84 KK31 pKa = 8.91 VYY33 pKa = 9.24 KK34 pKa = 10.27 QLMLYY39 pKa = 10.01 TSFGKK44 pKa = 10.39 VNKK47 pKa = 9.83 FLGLQHH53 pKa = 6.92 ICPKK57 pKa = 9.4 KK58 pKa = 9.8 VKK60 pKa = 9.83 YY61 pKa = 10.4 KK62 pKa = 10.98 LFFEE66 pKa = 4.74 VSLGPRR72 pKa = 11.84 IADD75 pKa = 3.51 IIVLASQGEE84 pKa = 3.85 QRR86 pKa = 11.84 MCYY89 pKa = 9.71 IIEE92 pKa = 4.52 LKK94 pKa = 9.51 TCLGAHH100 pKa = 6.32 FVPNPIKK107 pKa = 10.48 LAQRR111 pKa = 11.84 SQGLCQLHH119 pKa = 7.5 DD120 pKa = 3.69 STKK123 pKa = 9.85 YY124 pKa = 10.34 LCNNAPRR131 pKa = 11.84 GEE133 pKa = 4.21 EE134 pKa = 3.58 RR135 pKa = 11.84 WVVQAHH141 pKa = 6.92 LLFKK145 pKa = 10.84 SQVSLKK151 pKa = 8.68 TLHH154 pKa = 6.55 SEE156 pKa = 3.93 HH157 pKa = 6.69 PTFPFALIQSKK168 pKa = 10.72 EE169 pKa = 3.96 EE170 pKa = 3.81 ALSAFLRR177 pKa = 11.84 LRR179 pKa = 11.84 EE180 pKa = 4.09 DD181 pKa = 3.82 AEE183 pKa = 4.43 FKK185 pKa = 10.45 QVLRR189 pKa = 11.84 QGCPPAEE196 pKa = 4.26 VAKK199 pKa = 10.49 GMRR202 pKa = 11.84 LLGPKK207 pKa = 8.36 PQEE210 pKa = 3.73 RR211 pKa = 11.84 ARR213 pKa = 11.84 YY214 pKa = 8.63 KK215 pKa = 9.85 PPKK218 pKa = 9.48 VPRR221 pKa = 11.84 AQAQRR226 pKa = 11.84 LALPKK231 pKa = 10.57 ARR233 pKa = 11.84 GRR235 pKa = 11.84 KK236 pKa = 8.88 QNASHH241 pKa = 7.12 KK242 pKa = 10.31 KK243 pKa = 9.06 GAVKK247 pKa = 10.68 NGAA250 pKa = 3.54
Molecular weight: 28.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.246
IPC2_protein 9.575
IPC_protein 9.648
Toseland 10.584
ProMoST 10.087
Dawson 10.672
Bjellqvist 10.277
Wikipedia 10.789
Rodwell 11.228
Grimsley 10.701
Solomon 10.701
Lehninger 10.687
Nozaki 10.57
DTASelect 10.262
Thurlkill 10.555
EMBOSS 10.95
Sillero 10.584
Patrickios 10.935
IPC_peptide 10.716
IPC2_peptide 9.077
IPC2.peptide.svr19 8.551
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
74
0
74
35095
61
2624
474.3
52.89
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.106 ± 0.23
2.499 ± 0.185
4.36 ± 0.127
5.759 ± 0.411
4.499 ± 0.228
5.271 ± 0.506
2.764 ± 0.095
4.79 ± 0.163
4.739 ± 0.17
10.794 ± 0.287
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.117 ± 0.104
4.277 ± 0.171
6.517 ± 0.437
4.251 ± 0.188
4.502 ± 0.172
8.619 ± 0.26
5.927 ± 0.182
6.585 ± 0.206
1.091 ± 0.056
3.522 ± 0.139
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here