Wuhan pillworm virus 3

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KF15|A0A1L3KF15_9VIRU RNA-directed RNA polymerase OS=Wuhan pillworm virus 3 OX=1923746 PE=4 SV=1
MM1 pKa = 8.0RR2 pKa = 11.84DD3 pKa = 3.36SDD5 pKa = 4.5PPSKK9 pKa = 10.84DD10 pKa = 2.74IARR13 pKa = 11.84EE14 pKa = 3.82ALFRR18 pKa = 11.84TLSDD22 pKa = 3.4FRR24 pKa = 11.84HH25 pKa = 5.75LQRR28 pKa = 11.84DD29 pKa = 4.31CDD31 pKa = 4.07PEE33 pKa = 4.25WYY35 pKa = 10.08SDD37 pKa = 3.36EE38 pKa = 4.72NMDD41 pKa = 5.26RR42 pKa = 11.84VINSLNRR49 pKa = 11.84NASPGNPFCWVANNGQLIDD68 pKa = 3.53KK69 pKa = 10.28HH70 pKa = 7.49RR71 pKa = 11.84EE72 pKa = 3.74LLKK75 pKa = 9.68TLVRR79 pKa = 11.84QRR81 pKa = 11.84LNGASAYY88 pKa = 9.36PIRR91 pKa = 11.84LFVKK95 pKa = 9.98PEE97 pKa = 3.41WHH99 pKa = 6.56KK100 pKa = 10.79KK101 pKa = 9.48SKK103 pKa = 10.39IEE105 pKa = 3.77EE106 pKa = 3.85GRR108 pKa = 11.84YY109 pKa = 8.89RR110 pKa = 11.84LIWSVSVVDD119 pKa = 4.76QIIDD123 pKa = 3.35KK124 pKa = 9.87MLLGDD129 pKa = 4.53YY130 pKa = 10.68LDD132 pKa = 4.22QEE134 pKa = 4.17PDD136 pKa = 2.48NWIFHH141 pKa = 6.12PSKK144 pKa = 11.02VGWSYY149 pKa = 11.81LKK151 pKa = 10.92GGWKK155 pKa = 9.76YY156 pKa = 10.1VPKK159 pKa = 10.78GVGTDD164 pKa = 4.13FSAWDD169 pKa = 3.4MTAQQWLLDD178 pKa = 4.19LLCEE182 pKa = 4.7FYY184 pKa = 10.68INQHH188 pKa = 6.09RR189 pKa = 11.84NPTAYY194 pKa = 8.86WISWVRR200 pKa = 11.84RR201 pKa = 11.84RR202 pKa = 11.84FGEE205 pKa = 4.35LYY207 pKa = 10.55HH208 pKa = 7.2GGALVQLSDD217 pKa = 3.64GTLLEE222 pKa = 4.01QLFGGIQKK230 pKa = 10.56SGTLYY235 pKa = 9.89TLSGNSIMQVILHH248 pKa = 5.95HH249 pKa = 6.19VICLSLNYY257 pKa = 10.46DD258 pKa = 3.68SVPWLWVLGDD268 pKa = 4.28DD269 pKa = 4.4RR270 pKa = 11.84LQEE273 pKa = 4.26RR274 pKa = 11.84VDD276 pKa = 3.63KK277 pKa = 10.75EE278 pKa = 3.86YY279 pKa = 11.52ADD281 pKa = 3.62ALKK284 pKa = 10.44RR285 pKa = 11.84WVVLKK290 pKa = 10.54DD291 pKa = 2.74ISYY294 pKa = 11.02NEE296 pKa = 4.35FCGMHH301 pKa = 6.03YY302 pKa = 10.81GSTIEE307 pKa = 3.48PAYY310 pKa = 10.27RR311 pKa = 11.84SKK313 pKa = 11.16HH314 pKa = 5.39LLNLAADD321 pKa = 4.15PTPEE325 pKa = 4.05RR326 pKa = 11.84LMSYY330 pKa = 9.99QLLYY334 pKa = 10.27RR335 pKa = 11.84HH336 pKa = 6.15SKK338 pKa = 10.2FYY340 pKa = 9.9PALVRR345 pKa = 11.84FIRR348 pKa = 11.84SLNVQPAHH356 pKa = 6.5EE357 pKa = 4.19IWVDD361 pKa = 3.98EE362 pKa = 4.29VWGG365 pKa = 3.85

Molecular weight: 42.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KF15|A0A1L3KF15_9VIRU RNA-directed RNA polymerase OS=Wuhan pillworm virus 3 OX=1923746 PE=4 SV=1
MM1 pKa = 7.38NAQTKK6 pKa = 10.29KK7 pKa = 8.92ITCHH11 pKa = 5.17ACGRR15 pKa = 11.84RR16 pKa = 11.84FNTQQALHH24 pKa = 5.15QHH26 pKa = 4.8QQAVHH31 pKa = 6.07GAANQRR37 pKa = 11.84VPRR40 pKa = 11.84GAGRR44 pKa = 11.84GGRR47 pKa = 11.84GRR49 pKa = 11.84RR50 pKa = 11.84NGQNNQGPARR60 pKa = 11.84QGTAQSVTSRR70 pKa = 11.84ATPNQGVSVSGFEE83 pKa = 4.07RR84 pKa = 11.84LGSIDD89 pKa = 3.22IVNAASASWQVNAWMTPRR107 pKa = 11.84LQAMSSAFQRR117 pKa = 11.84IHH119 pKa = 5.95YY120 pKa = 10.12LSLKK124 pKa = 9.14VNVVAFGSAIGTGGYY139 pKa = 10.37VIGFVADD146 pKa = 4.17PSDD149 pKa = 3.75ATPTISQLQSQAGARR164 pKa = 11.84TCKK167 pKa = 10.05SWEE170 pKa = 4.04NCDD173 pKa = 3.38VPLVVPGTLYY183 pKa = 9.48YY184 pKa = 10.2TSRR187 pKa = 11.84DD188 pKa = 3.61NEE190 pKa = 4.11PRR192 pKa = 11.84WYY194 pKa = 10.01SPGRR198 pKa = 11.84IVILVDD204 pKa = 4.9GIASAGGKK212 pKa = 9.59LVINLHH218 pKa = 5.08WSVRR222 pKa = 11.84LSVPTATPPTVLKK235 pKa = 10.78DD236 pKa = 3.29IFLSEE241 pKa = 4.28HH242 pKa = 6.79LYY244 pKa = 10.73VIPGKK249 pKa = 10.39NILGWKK255 pKa = 10.12DD256 pKa = 3.03GTTFRR261 pKa = 11.84GNISKK266 pKa = 10.16FLSTPVPGDD275 pKa = 2.79SWLSVPTFGIEE286 pKa = 3.98YY287 pKa = 10.22NEE289 pKa = 4.4GVGDD293 pKa = 3.57TGTILIHH300 pKa = 4.89YY301 pKa = 7.62LKK303 pKa = 10.32YY304 pKa = 10.52NSSEE308 pKa = 4.01GSLTCSNDD316 pKa = 3.06GEE318 pKa = 4.49KK319 pKa = 10.75VVTQVWQASVAAQVVVPRR337 pKa = 11.84NTILTIVDD345 pKa = 3.56YY346 pKa = 11.19NKK348 pKa = 9.44PAKK351 pKa = 9.75PAVSDD356 pKa = 4.41FIQPLVSNFNNVGIDD371 pKa = 3.31SSRR374 pKa = 11.84NSTTPSAGEE383 pKa = 4.1TGTDD387 pKa = 3.63PPYY390 pKa = 10.2STYY393 pKa = 11.26LEE395 pKa = 4.05LLRR398 pKa = 11.84RR399 pKa = 11.84QSALDD404 pKa = 3.54SKK406 pKa = 10.73ISRR409 pKa = 11.84IEE411 pKa = 3.87LQLSKK416 pKa = 9.99MVKK419 pKa = 9.83VLMPKK424 pKa = 10.38DD425 pKa = 3.56QDD427 pKa = 3.54SSQSSRR433 pKa = 11.84SRR435 pKa = 11.84SPSKK439 pKa = 10.45DD440 pKa = 3.01TTKK443 pKa = 10.77LDD445 pKa = 3.77SKK447 pKa = 10.8GTT449 pKa = 3.68

Molecular weight: 48.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1319

365

505

439.7

49.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.534 ± 0.697

1.061 ± 0.14

5.914 ± 0.646

3.563 ± 0.552

3.487 ± 0.573

7.354 ± 0.611

2.35 ± 0.339

5.838 ± 0.44

5.459 ± 0.647

9.249 ± 1.103

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.592 ± 0.209

4.397 ± 0.479

5.004 ± 0.349

5.08 ± 0.373

5.838 ± 0.398

9.325 ± 0.841

5.762 ± 1.09

6.596 ± 0.894

2.957 ± 0.58

3.639 ± 0.501
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski