Amino acid dipepetide frequency for Rhodopirellula sp. SM50

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.878AlaAla: 11.878 ± 0.106
1.042AlaCys: 1.042 ± 0.026
6.942AlaAsp: 6.942 ± 0.07
6.594AlaGlu: 6.594 ± 0.078
3.339AlaPhe: 3.339 ± 0.038
8.376AlaGly: 8.376 ± 0.091
1.483AlaHis: 1.483 ± 0.032
5.869AlaIle: 5.869 ± 0.058
3.517AlaLys: 3.517 ± 0.063
8.103AlaLeu: 8.103 ± 0.079
2.501AlaMet: 2.501 ± 0.045
2.956AlaAsn: 2.956 ± 0.043
4.188AlaPro: 4.188 ± 0.057
3.054AlaGln: 3.054 ± 0.04
5.351AlaArg: 5.351 ± 0.067
6.862AlaSer: 6.862 ± 0.076
6.288AlaThr: 6.288 ± 0.07
6.961AlaVal: 6.961 ± 0.057
1.273AlaTrp: 1.273 ± 0.027
1.989AlaTyr: 1.989 ± 0.029
0.0AlaXaa: 0.0 ± 0.0
Cys
0.685CysAla: 0.685 ± 0.022
0.216CysCys: 0.216 ± 0.011
0.764CysAsp: 0.764 ± 0.024
0.602CysGlu: 0.602 ± 0.018
0.415CysPhe: 0.415 ± 0.015
0.989CysGly: 0.989 ± 0.03
0.418CysHis: 0.418 ± 0.02
0.396CysIle: 0.396 ± 0.013
0.277CysLys: 0.277 ± 0.012
0.989CysLeu: 0.989 ± 0.026
0.176CysMet: 0.176 ± 0.009
0.284CysAsn: 0.284 ± 0.011
0.53CysPro: 0.53 ± 0.017
0.402CysGln: 0.402 ± 0.014
0.783CysArg: 0.783 ± 0.022
0.646CysSer: 0.646 ± 0.02
0.478CysThr: 0.478 ± 0.018
0.822CysVal: 0.822 ± 0.02
0.161CysTrp: 0.161 ± 0.009
0.281CysTyr: 0.281 ± 0.01
0.0CysXaa: 0.0 ± 0.0
Asp
7.481AspAla: 7.481 ± 0.065
0.571AspCys: 0.571 ± 0.017
5.009AspAsp: 5.009 ± 0.072
4.333AspGlu: 4.333 ± 0.04
2.568AspPhe: 2.568 ± 0.045
6.634AspGly: 6.634 ± 0.137
1.646AspHis: 1.646 ± 0.027
2.379AspIle: 2.379 ± 0.04
1.577AspLys: 1.577 ± 0.031
6.417AspLeu: 6.417 ± 0.074
0.914AspMet: 0.914 ± 0.021
1.778AspAsn: 1.778 ± 0.043
4.293AspPro: 4.293 ± 0.054
3.219AspGln: 3.219 ± 0.036
5.022AspArg: 5.022 ± 0.047
4.359AspSer: 4.359 ± 0.064
2.922AspThr: 2.922 ± 0.066
5.017AspVal: 5.017 ± 0.06
1.126AspTrp: 1.126 ± 0.02
1.732AspTyr: 1.732 ± 0.03
0.0AspXaa: 0.0 ± 0.0
Glu
5.254GluAla: 5.254 ± 0.062
0.481GluCys: 0.481 ± 0.015
2.881GluAsp: 2.881 ± 0.043
2.798GluGlu: 2.798 ± 0.049
2.309GluPhe: 2.309 ± 0.03
3.505GluGly: 3.505 ± 0.044
1.319GluHis: 1.319 ± 0.024
3.496GluIle: 3.496 ± 0.043
2.16GluLys: 2.16 ± 0.045
6.562GluLeu: 6.562 ± 0.073
1.442GluMet: 1.442 ± 0.032
1.973GluAsn: 1.973 ± 0.029
2.746GluPro: 2.746 ± 0.039
2.907GluGln: 2.907 ± 0.046
3.864GluArg: 3.864 ± 0.062
4.722GluSer: 4.722 ± 0.05
4.021GluThr: 4.021 ± 0.042
3.855GluVal: 3.855 ± 0.042
0.61GluTrp: 0.61 ± 0.015
1.253GluTyr: 1.253 ± 0.023
0.0GluXaa: 0.0 ± 0.0
Phe
3.749PheAla: 3.749 ± 0.039
0.45PheCys: 0.45 ± 0.013
3.176PheAsp: 3.176 ± 0.051
2.274PheGlu: 2.274 ± 0.034
1.404PhePhe: 1.404 ± 0.028
3.363PheGly: 3.363 ± 0.045
0.803PheHis: 0.803 ± 0.019
1.35PheIle: 1.35 ± 0.022
0.921PheLys: 0.921 ± 0.021
3.277PheLeu: 3.277 ± 0.04
0.528PheMet: 0.528 ± 0.014
1.166PheAsn: 1.166 ± 0.028
1.554PhePro: 1.554 ± 0.024
1.456PheGln: 1.456 ± 0.022
2.367PheArg: 2.367 ± 0.033
2.484PheSer: 2.484 ± 0.034
2.028PheThr: 2.028 ± 0.052
2.865PheVal: 2.865 ± 0.039
0.519PheTrp: 0.519 ± 0.015
0.907PheTyr: 0.907 ± 0.02
0.0PheXaa: 0.0 ± 0.0
Gly
6.19GlyAla: 6.19 ± 0.082
0.952GlyCys: 0.952 ± 0.028
5.893GlyAsp: 5.893 ± 0.112
4.716GlyGlu: 4.716 ± 0.049
3.125GlyPhe: 3.125 ± 0.046
7.161GlyGly: 7.161 ± 0.146
1.686GlyHis: 1.686 ± 0.028
4.064GlyIle: 4.064 ± 0.055
3.248GlyLys: 3.248 ± 0.05
6.866GlyLeu: 6.866 ± 0.059
1.783GlyMet: 1.783 ± 0.04
3.056GlyAsn: 3.056 ± 0.093
3.235GlyPro: 3.235 ± 0.038
3.418GlyGln: 3.418 ± 0.04
5.381GlyArg: 5.381 ± 0.059
5.337GlySer: 5.337 ± 0.093
4.929GlyThr: 4.929 ± 0.089
5.608GlyVal: 5.608 ± 0.052
1.252GlyTrp: 1.252 ± 0.026
2.052GlyTyr: 2.052 ± 0.027
0.0GlyXaa: 0.0 ± 0.0
His
2.059HisAla: 2.059 ± 0.039
0.333HisCys: 0.333 ± 0.013
1.38HisAsp: 1.38 ± 0.023
1.106HisGlu: 1.106 ± 0.023
0.899HisPhe: 0.899 ± 0.019
1.743HisGly: 1.743 ± 0.027
0.702HisHis: 0.702 ± 0.02
0.686HisIle: 0.686 ± 0.017
0.449HisLys: 0.449 ± 0.014
2.188HisLeu: 2.188 ± 0.035
0.317HisMet: 0.317 ± 0.012
0.599HisAsn: 0.599 ± 0.016
1.448HisPro: 1.448 ± 0.028
1.026HisGln: 1.026 ± 0.023
1.74HisArg: 1.74 ± 0.038
1.29HisSer: 1.29 ± 0.025
0.931HisThr: 0.931 ± 0.019
1.539HisVal: 1.539 ± 0.023
0.446HisTrp: 0.446 ± 0.016
0.624HisTyr: 0.624 ± 0.017
0.0HisXaa: 0.0 ± 0.0
Ile
6.038IleAla: 6.038 ± 0.05
0.539IleCys: 0.539 ± 0.016
4.682IleAsp: 4.682 ± 0.059
3.856IleGlu: 3.856 ± 0.036
1.31IlePhe: 1.31 ± 0.023
4.451IleGly: 4.451 ± 0.048
1.077IleHis: 1.077 ± 0.022
1.752IleIle: 1.752 ± 0.031
1.431IleLys: 1.431 ± 0.029
3.796IleLeu: 3.796 ± 0.047
0.601IleMet: 0.601 ± 0.017
1.648IleAsn: 1.648 ± 0.036
2.403IlePro: 2.403 ± 0.031
1.805IleGln: 1.805 ± 0.028
3.569IleArg: 3.569 ± 0.044
3.002IleSer: 3.002 ± 0.041
2.77IleThr: 2.77 ± 0.06
3.892IleVal: 3.892 ± 0.04
0.611IleTrp: 0.611 ± 0.014
1.078IleTyr: 1.078 ± 0.023
0.0IleXaa: 0.0 ± 0.0
Lys
2.681LysAla: 2.681 ± 0.051
0.257LysCys: 0.257 ± 0.011
1.563LysAsp: 1.563 ± 0.03
1.576LysGlu: 1.576 ± 0.042
0.983LysPhe: 0.983 ± 0.021
1.81LysGly: 1.81 ± 0.033
0.763LysHis: 0.763 ± 0.02
1.656LysIle: 1.656 ± 0.031
1.362LysLys: 1.362 ± 0.034
3.469LysLeu: 3.469 ± 0.053
0.746LysMet: 0.746 ± 0.017
0.969LysAsn: 0.969 ± 0.022
2.006LysPro: 2.006 ± 0.04
1.628LysGln: 1.628 ± 0.035
2.498LysArg: 2.498 ± 0.043
2.12LysSer: 2.12 ± 0.032
2.08LysThr: 2.08 ± 0.035
2.063LysVal: 2.063 ± 0.036
0.483LysTrp: 0.483 ± 0.016
0.742LysTyr: 0.742 ± 0.016
0.0LysXaa: 0.0 ± 0.0
Leu
10.734LeuAla: 10.734 ± 0.097
1.034LeuCys: 1.034 ± 0.027
6.463LeuAsp: 6.463 ± 0.053
5.216LeuGlu: 5.216 ± 0.062
3.273LeuPhe: 3.273 ± 0.038
7.027LeuGly: 7.027 ± 0.055
1.937LeuHis: 1.937 ± 0.034
4.828LeuIle: 4.828 ± 0.047
3.127LeuLys: 3.127 ± 0.049
9.067LeuLeu: 9.067 ± 0.089
1.902LeuMet: 1.902 ± 0.038
2.801LeuAsn: 2.801 ± 0.038
5.248LeuPro: 5.248 ± 0.05
3.684LeuGln: 3.684 ± 0.042
6.435LeuArg: 6.435 ± 0.061
6.692LeuSer: 6.692 ± 0.06
5.612LeuThr: 5.612 ± 0.081
7.038LeuVal: 7.038 ± 0.068
1.148LeuTrp: 1.148 ± 0.024
2.023LeuTyr: 2.023 ± 0.026
0.0LeuXaa: 0.0 ± 0.0
Met
1.914MetAla: 1.914 ± 0.036
0.17MetCys: 0.17 ± 0.009
1.035MetAsp: 1.035 ± 0.022
1.027MetGlu: 1.027 ± 0.023
0.649MetPhe: 0.649 ± 0.016
1.348MetGly: 1.348 ± 0.031
0.448MetHis: 0.448 ± 0.014
1.195MetIle: 1.195 ± 0.025
0.881MetLys: 0.881 ± 0.024
2.164MetLeu: 2.164 ± 0.038
0.565MetMet: 0.565 ± 0.016
0.787MetAsn: 0.787 ± 0.018
1.277MetPro: 1.277 ± 0.024
0.892MetGln: 0.892 ± 0.023
1.416MetArg: 1.416 ± 0.03
1.314MetSer: 1.314 ± 0.026
1.444MetThr: 1.444 ± 0.026
1.428MetVal: 1.428 ± 0.027
0.201MetTrp: 0.201 ± 0.01
0.329MetTyr: 0.329 ± 0.011
0.0MetXaa: 0.0 ± 0.0
Asn
3.09AsnAla: 3.09 ± 0.048
0.311AsnCys: 0.311 ± 0.013
2.267AsnAsp: 2.267 ± 0.059
1.877AsnGlu: 1.877 ± 0.031
1.048AsnPhe: 1.048 ± 0.028
2.762AsnGly: 2.762 ± 0.066
0.756AsnHis: 0.756 ± 0.018
1.201AsnIle: 1.201 ± 0.025
0.698AsnLys: 0.698 ± 0.015
3.006AsnLeu: 3.006 ± 0.041
0.468AsnMet: 0.468 ± 0.013
1.035AsnAsn: 1.035 ± 0.036
2.002AsnPro: 2.002 ± 0.031
1.497AsnGln: 1.497 ± 0.039
2.408AsnArg: 2.408 ± 0.034
1.808AsnSer: 1.808 ± 0.043
1.598AsnThr: 1.598 ± 0.047
2.406AsnVal: 2.406 ± 0.04
0.465AsnTrp: 0.465 ± 0.013
0.77AsnTyr: 0.77 ± 0.019
0.0AsnXaa: 0.0 ± 0.0
Pro
5.21ProAla: 5.21 ± 0.067
0.363ProCys: 0.363 ± 0.014
3.905ProAsp: 3.905 ± 0.045
3.492ProGlu: 3.492 ± 0.045
1.744ProPhe: 1.744 ± 0.023
4.111ProGly: 4.111 ± 0.045
1.091ProHis: 1.091 ± 0.025
2.849ProIle: 2.849 ± 0.034
1.688ProLys: 1.688 ± 0.037
4.449ProLeu: 4.449 ± 0.043
1.113ProMet: 1.113 ± 0.022
1.805ProAsn: 1.805 ± 0.028
3.048ProPro: 3.048 ± 0.052
1.957ProGln: 1.957 ± 0.032
2.749ProArg: 2.749 ± 0.037
3.711ProSer: 3.711 ± 0.044
3.345ProThr: 3.345 ± 0.043
3.756ProVal: 3.756 ± 0.038
0.681ProTrp: 0.681 ± 0.015
1.077ProTyr: 1.077 ± 0.022
0.0ProXaa: 0.0 ± 0.0
Gln
3.948GlnAla: 3.948 ± 0.049
0.389GlnCys: 0.389 ± 0.014
1.89GlnAsp: 1.89 ± 0.025
1.672GlnGlu: 1.672 ± 0.031
1.587GlnPhe: 1.587 ± 0.026
2.426GlnGly: 2.426 ± 0.035
0.93GlnHis: 0.93 ± 0.02
2.366GlnIle: 2.366 ± 0.033
1.156GlnLys: 1.156 ± 0.024
4.486GlnLeu: 4.486 ± 0.049
0.912GlnMet: 0.912 ± 0.022
1.211GlnAsn: 1.211 ± 0.023
2.396GlnPro: 2.396 ± 0.037
2.081GlnGln: 2.081 ± 0.038
3.216GlnArg: 3.216 ± 0.05
3.13GlnSer: 3.13 ± 0.043
2.73GlnThr: 2.73 ± 0.048
2.689GlnVal: 2.689 ± 0.032
0.753GlnTrp: 0.753 ± 0.017
0.949GlnTyr: 0.949 ± 0.023
0.0GlnXaa: 0.0 ± 0.0
Arg
4.917ArgAla: 4.917 ± 0.055
0.793ArgCys: 0.793 ± 0.025
4.359ArgAsp: 4.359 ± 0.046
3.774ArgGlu: 3.774 ± 0.06
3.03ArgPhe: 3.03 ± 0.039
4.502ArgGly: 4.502 ± 0.051
1.597ArgHis: 1.597 ± 0.029
3.676ArgIle: 3.676 ± 0.037
2.172ArgLys: 2.172 ± 0.041
7.285ArgLeu: 7.285 ± 0.081
1.675ArgMet: 1.675 ± 0.033
2.114ArgAsn: 2.114 ± 0.027
3.234ArgPro: 3.234 ± 0.047
3.097ArgGln: 3.097 ± 0.051
5.543ArgArg: 5.543 ± 0.09
4.569ArgSer: 4.569 ± 0.049
3.27ArgThr: 3.27 ± 0.039
4.695ArgVal: 4.695 ± 0.044
1.313ArgTrp: 1.313 ± 0.028
1.931ArgTyr: 1.931 ± 0.027
0.0ArgXaa: 0.0 ± 0.0
Ser
6.129SerAla: 6.129 ± 0.057
0.59SerCys: 0.59 ± 0.019
4.981SerAsp: 4.981 ± 0.063
3.829SerGlu: 3.829 ± 0.045
2.501SerPhe: 2.501 ± 0.043
6.54SerGly: 6.54 ± 0.113
1.324SerHis: 1.324 ± 0.025
3.683SerIle: 3.683 ± 0.047
1.96SerLys: 1.96 ± 0.036
6.581SerLeu: 6.581 ± 0.064
1.443SerMet: 1.443 ± 0.026
2.118SerAsn: 2.118 ± 0.048
3.703SerPro: 3.703 ± 0.052
2.51SerGln: 2.51 ± 0.037
4.092SerArg: 4.092 ± 0.049
4.374SerSer: 4.374 ± 0.093
3.77SerThr: 3.77 ± 0.075
5.122SerVal: 5.122 ± 0.058
0.86SerTrp: 0.86 ± 0.018
1.412SerTyr: 1.412 ± 0.026
0.0SerXaa: 0.0 ± 0.0
Thr
5.785ThrAla: 5.785 ± 0.06
0.475ThrCys: 0.475 ± 0.014
3.905ThrAsp: 3.905 ± 0.071
3.138ThrGlu: 3.138 ± 0.044
2.192ThrPhe: 2.192 ± 0.048
4.963ThrGly: 4.963 ± 0.088
1.125ThrHis: 1.125 ± 0.019
3.419ThrIle: 3.419 ± 0.066
1.644ThrLys: 1.644 ± 0.029
6.123ThrLeu: 6.123 ± 0.071
1.108ThrMet: 1.108 ± 0.023
1.786ThrAsn: 1.786 ± 0.048
3.609ThrPro: 3.609 ± 0.044
1.92ThrGln: 1.92 ± 0.029
3.348ThrArg: 3.348 ± 0.041
3.737ThrSer: 3.737 ± 0.085
3.581ThrThr: 3.581 ± 0.107
4.549ThrVal: 4.549 ± 0.08
0.781ThrTrp: 0.781 ± 0.018
1.359ThrTyr: 1.359 ± 0.036
0.0ThrXaa: 0.0 ± 0.0
Val
7.718ValAla: 7.718 ± 0.061
0.867ValCys: 0.867 ± 0.022
5.355ValAsp: 5.355 ± 0.07
4.227ValGlu: 4.227 ± 0.04
2.705ValPhe: 2.705 ± 0.039
5.438ValGly: 5.438 ± 0.047
1.409ValHis: 1.409 ± 0.026
3.825ValIle: 3.825 ± 0.04
2.036ValLys: 2.036 ± 0.034
6.619ValLeu: 6.619 ± 0.055
1.533ValMet: 1.533 ± 0.029
2.16ValAsn: 2.16 ± 0.043
3.449ValPro: 3.449 ± 0.037
2.488ValGln: 2.488 ± 0.032
4.691ValArg: 4.691 ± 0.041
4.957ValSer: 4.957 ± 0.062
4.732ValThr: 4.732 ± 0.09
5.819ValVal: 5.819 ± 0.065
0.982ValTrp: 0.982 ± 0.023
1.671ValTyr: 1.671 ± 0.032
0.0ValXaa: 0.0 ± 0.0
Trp
1.023TrpAla: 1.023 ± 0.021
0.177TrpCys: 0.177 ± 0.009
0.836TrpAsp: 0.836 ± 0.021
0.666TrpGlu: 0.666 ± 0.017
0.564TrpPhe: 0.564 ± 0.015
0.866TrpGly: 0.866 ± 0.02
0.39TrpHis: 0.39 ± 0.012
0.908TrpIle: 0.908 ± 0.02
0.603TrpLys: 0.603 ± 0.019
1.531TrpLeu: 1.531 ± 0.03
0.415TrpMet: 0.415 ± 0.013
0.558TrpAsn: 0.558 ± 0.014
0.652TrpPro: 0.652 ± 0.019
0.714TrpGln: 0.714 ± 0.018
0.985TrpArg: 0.985 ± 0.022
1.016TrpSer: 1.016 ± 0.02
0.89TrpThr: 0.89 ± 0.021
0.914TrpVal: 0.914 ± 0.02
0.245TrpTrp: 0.245 ± 0.012
0.355TrpTyr: 0.355 ± 0.011
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.083TyrAla: 2.083 ± 0.026
0.303TyrCys: 0.303 ± 0.011
1.711TyrAsp: 1.711 ± 0.041
1.384TyrGlu: 1.384 ± 0.024
0.993TyrPhe: 0.993 ± 0.022
1.938TyrGly: 1.938 ± 0.033
0.602TyrHis: 0.602 ± 0.016
0.713TyrIle: 0.713 ± 0.018
0.548TyrLys: 0.548 ± 0.016
2.314TyrLeu: 2.314 ± 0.029
0.34TyrMet: 0.34 ± 0.013
0.669TyrAsn: 0.669 ± 0.019
1.124TyrPro: 1.124 ± 0.024
1.163TyrGln: 1.163 ± 0.023
2.09TyrArg: 2.09 ± 0.03
1.391TyrSer: 1.391 ± 0.027
1.129TyrThr: 1.129 ± 0.035
1.642TyrVal: 1.642 ± 0.029
0.396TyrTrp: 0.396 ± 0.014
0.66TyrTyr: 0.66 ± 0.02
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 6414 proteins (2788521 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski