Merismopedia glauca CCAP 1448/3
Average proteome isoelectric point is 6.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4638 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2T1C4B0|A0A2T1C4B0_9CYAN TonB_C domain-containing protein OS=Merismopedia glauca CCAP 1448/3 OX=1296344 GN=C7B64_10150 PE=4 SV=1
MM1 pKa = 8.14 DD2 pKa = 5.01 DD3 pKa = 3.05 WSKK6 pKa = 11.16 EE7 pKa = 3.59 FWQAIDD13 pKa = 3.86 DD14 pKa = 5.13 AILAVDD20 pKa = 3.99 RR21 pKa = 11.84 VFTGVATTLEE31 pKa = 4.28 TAVDD35 pKa = 3.94 DD36 pKa = 4.9 LLMEE40 pKa = 4.15 VATNVEE46 pKa = 4.26 STVADD51 pKa = 3.53 VQKK54 pKa = 10.69 IIVSEE59 pKa = 3.9 LDD61 pKa = 3.33 EE62 pKa = 5.01 NVPEE66 pKa = 3.93 WRR68 pKa = 11.84 EE69 pKa = 3.85 WLDD72 pKa = 3.53 GDD74 pKa = 5.79 DD75 pKa = 6.07 DD76 pKa = 5.07 LFPDD80 pKa = 3.59 MEE82 pKa = 4.62 EE83 pKa = 4.54 FNHH86 pKa = 5.72 HH87 pKa = 5.06 TEE89 pKa = 4.49 FYY91 pKa = 10.61 DD92 pKa = 3.57 VYY94 pKa = 10.53 PVFPSAEE101 pKa = 4.08 NNPACIGCRR110 pKa = 11.84 NYY112 pKa = 10.38 HH113 pKa = 4.62 GQVYY117 pKa = 10.22 NGNILVCGMHH127 pKa = 6.3 PSGWEE132 pKa = 3.98 DD133 pKa = 3.48 EE134 pKa = 4.65 KK135 pKa = 11.71 CPDD138 pKa = 3.36 WEE140 pKa = 4.84 SNN142 pKa = 3.31
Molecular weight: 16.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.707
IPC2_protein 3.77
IPC_protein 3.757
Toseland 3.554
ProMoST 3.91
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.668
Rodwell 3.579
Grimsley 3.465
Solomon 3.732
Lehninger 3.681
Nozaki 3.859
DTASelect 4.062
Thurlkill 3.605
EMBOSS 3.681
Sillero 3.872
Patrickios 1.036
IPC_peptide 3.732
IPC2_peptide 3.859
IPC2.peptide.svr19 3.787
Protein with the highest isoelectric point:
>tr|A0A2T1C8W3|A0A2T1C8W3_9CYAN Uncharacterized protein OS=Merismopedia glauca CCAP 1448/3 OX=1296344 GN=C7B64_02525 PE=4 SV=1
MM1 pKa = 7.29 TRR3 pKa = 11.84 RR4 pKa = 11.84 TLEE7 pKa = 3.73 GTNRR11 pKa = 11.84 KK12 pKa = 7.62 QKK14 pKa = 8.83 RR15 pKa = 11.84 TSGFRR20 pKa = 11.84 ARR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 NGRR29 pKa = 11.84 KK30 pKa = 8.47 VIKK33 pKa = 10.05 ARR35 pKa = 11.84 RR36 pKa = 11.84 VKK38 pKa = 10.19 GRR40 pKa = 11.84 YY41 pKa = 9.18 RR42 pKa = 11.84 LTVV45 pKa = 2.95
Molecular weight: 5.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.371
IPC2_protein 10.935
IPC_protein 12.369
Toseland 12.53
ProMoST 13.027
Dawson 12.53
Bjellqvist 12.53
Wikipedia 13.013
Rodwell 12.193
Grimsley 12.574
Solomon 13.027
Lehninger 12.925
Nozaki 12.53
DTASelect 12.53
Thurlkill 12.53
EMBOSS 13.027
Sillero 12.53
Patrickios 11.93
IPC_peptide 13.027
IPC2_peptide 12.018
IPC2.peptide.svr19 9.094
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4638
0
4638
1433816
25
3642
309.1
34.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.995 ± 0.035
1.022 ± 0.013
4.882 ± 0.025
6.28 ± 0.036
3.834 ± 0.023
6.643 ± 0.041
1.822 ± 0.018
6.881 ± 0.026
4.948 ± 0.03
11.057 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.789 ± 0.016
4.27 ± 0.029
4.787 ± 0.027
5.3 ± 0.03
5.099 ± 0.029
6.702 ± 0.029
5.622 ± 0.028
6.497 ± 0.027
1.485 ± 0.016
3.086 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here