Serratia nematodiphila DZ0503SBS1
Average proteome isoelectric point is 6.89
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2715 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A086G9A7|A0A086G9A7_9GAMM Uncharacterized protein OS=Serratia nematodiphila DZ0503SBS1 OX=1449981 GN=AK51_25390 PE=3 SV=1
MM1 pKa = 7.62 FDD3 pKa = 2.9 VLMYY7 pKa = 11.04 LFEE10 pKa = 4.53 TYY12 pKa = 9.29 IHH14 pKa = 6.7 NEE16 pKa = 3.36 PEE18 pKa = 3.83 MRR20 pKa = 11.84 VDD22 pKa = 4.17 QDD24 pKa = 3.69 QLTDD28 pKa = 4.06 DD29 pKa = 4.58 LAQAGFHH36 pKa = 6.64 QDD38 pKa = 3.72 DD39 pKa = 4.92 IYY41 pKa = 11.84 NALNWLEE48 pKa = 4.3 KK49 pKa = 10.72 LADD52 pKa = 3.84 LQEE55 pKa = 4.58 GQNAPYY61 pKa = 10.61 FMDD64 pKa = 4.97 ADD66 pKa = 4.08 PLAMRR71 pKa = 11.84 IYY73 pKa = 10.25 TEE75 pKa = 4.22 EE76 pKa = 3.69 EE77 pKa = 3.8 NARR80 pKa = 11.84 LDD82 pKa = 3.63 AGCRR86 pKa = 11.84 GFLLFLEE93 pKa = 4.79 QIQVLNLEE101 pKa = 4.22 TRR103 pKa = 11.84 EE104 pKa = 3.96 MVIDD108 pKa = 3.93 RR109 pKa = 11.84 VMALDD114 pKa = 3.52 NAEE117 pKa = 4.78 FDD119 pKa = 4.9 LEE121 pKa = 4.12 DD122 pKa = 4.22 LKK124 pKa = 11.14 WVVLMVLFNIPGYY137 pKa = 10.27 EE138 pKa = 3.8 SAYY141 pKa = 10.17 QQMEE145 pKa = 4.03 EE146 pKa = 4.66 LLFEE150 pKa = 4.54 VNEE153 pKa = 5.12 GYY155 pKa = 10.8 LHH157 pKa = 7.0
Molecular weight: 18.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.757
IPC2_protein 3.872
IPC_protein 3.846
Toseland 3.643
ProMoST 3.973
Dawson 3.808
Bjellqvist 3.973
Wikipedia 3.719
Rodwell 3.668
Grimsley 3.554
Solomon 3.808
Lehninger 3.757
Nozaki 3.923
DTASelect 4.101
Thurlkill 3.681
EMBOSS 3.732
Sillero 3.961
Patrickios 1.875
IPC_peptide 3.808
IPC2_peptide 3.935
IPC2.peptide.svr19 3.857
Protein with the highest isoelectric point:
>tr|A0A1W9F104|A0A1W9F104_9GAMM Propionate catabolism operon regulatory protein OS=Serratia nematodiphila DZ0503SBS1 OX=1449981 GN=AK51_28565 PE=4 SV=1
CC1 pKa = 7.85 ILVCIFGSVYY11 pKa = 10.74 CLFSPRR17 pKa = 11.84 NPRR20 pKa = 11.84 HH21 pKa = 5.1 VATFGHH27 pKa = 6.7 LFGRR31 pKa = 11.84 LSTLFGIKK39 pKa = 10.14 VEE41 pKa = 4.31 TRR43 pKa = 11.84 VPADD47 pKa = 3.03 AANNGNCIYY56 pKa = 10.43 IANHH60 pKa = 4.79 QNNYY64 pKa = 11.15 DD65 pKa = 3.46 MVTASNIVQPRR76 pKa = 11.84 TVTVGKK82 pKa = 10.39 KK83 pKa = 10.21 SGVDD87 pKa = 3.3 PVFGPLYY94 pKa = 9.71 WLTGNLLIDD103 pKa = 3.8 RR104 pKa = 11.84 DD105 pKa = 3.87 NRR107 pKa = 11.84 AKK109 pKa = 9.72 RR110 pKa = 11.84 TAPSPRR116 pKa = 11.84 WRR118 pKa = 11.84 SSSKK122 pKa = 10.43 RR123 pKa = 11.84 KK124 pKa = 7.61 TFRR127 pKa = 11.84 SGCSRR132 pKa = 11.84 KK133 pKa = 10.13 DD134 pKa = 3.28 PQPRR138 pKa = 11.84 SWW140 pKa = 3.21
Molecular weight: 15.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.35
IPC2_protein 9.663
IPC_protein 10.35
Toseland 10.555
ProMoST 10.248
Dawson 10.687
Bjellqvist 10.394
Wikipedia 10.877
Rodwell 10.935
Grimsley 10.73
Solomon 10.76
Lehninger 10.73
Nozaki 10.584
DTASelect 10.379
Thurlkill 10.57
EMBOSS 10.95
Sillero 10.613
Patrickios 10.672
IPC_peptide 10.774
IPC2_peptide 9.648
IPC2.peptide.svr19 8.472
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2715
0
2715
493119
31
1074
181.6
20.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.829 ± 0.062
1.247 ± 0.02
5.009 ± 0.045
5.301 ± 0.051
3.674 ± 0.036
7.503 ± 0.056
2.317 ± 0.033
5.299 ± 0.043
3.689 ± 0.046
11.199 ± 0.074
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.715 ± 0.028
3.329 ± 0.033
4.723 ± 0.041
4.847 ± 0.051
6.499 ± 0.052
5.786 ± 0.045
4.986 ± 0.037
6.859 ± 0.045
1.473 ± 0.024
2.717 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here