Synechococcus phage S-B05

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 268 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y5P0R0|A0A4Y5P0R0_9CAUD Tail fiber-like protein OS=Synechococcus phage S-B05 OX=2484637 PE=4 SV=1
MM1 pKa = 7.67FIITVAGKK9 pKa = 9.43EE10 pKa = 4.01KK11 pKa = 10.08EE12 pKa = 4.23GAYY15 pKa = 10.35SVVDD19 pKa = 3.77EE20 pKa = 4.55QGEE23 pKa = 4.13QVLYY27 pKa = 10.28IFCEE31 pKa = 3.75EE32 pKa = 4.56DD33 pKa = 3.14DD34 pKa = 3.99AEE36 pKa = 4.39RR37 pKa = 11.84YY38 pKa = 9.63AIQLEE43 pKa = 4.19EE44 pKa = 4.12LDD46 pKa = 4.01YY47 pKa = 11.7PEE49 pKa = 4.55MNVLEE54 pKa = 4.41VDD56 pKa = 3.78DD57 pKa = 5.46EE58 pKa = 4.93IMIKK62 pKa = 8.12TCEE65 pKa = 4.14MHH67 pKa = 6.23DD68 pKa = 3.29HH69 pKa = 7.43RR70 pKa = 11.84YY71 pKa = 7.38TVITSDD77 pKa = 5.44DD78 pKa = 3.3IVIPPDD84 pKa = 3.31SEE86 pKa = 3.95YY87 pKa = 11.5DD88 pKa = 3.68SFQKK92 pKa = 10.81DD93 pKa = 2.91

Molecular weight: 10.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y5P116|A0A4Y5P116_9CAUD Endosialidase OS=Synechococcus phage S-B05 OX=2484637 PE=4 SV=1
MM1 pKa = 6.32TQKK4 pKa = 9.9MMRR7 pKa = 11.84LFNTVTEE14 pKa = 4.11ARR16 pKa = 11.84SYY18 pKa = 11.44LRR20 pKa = 11.84TNHH23 pKa = 5.57GMTIARR29 pKa = 11.84AKK31 pKa = 10.41QYY33 pKa = 10.95VDD35 pKa = 2.65NHH37 pKa = 4.39TTMKK41 pKa = 10.44NGNKK45 pKa = 9.0VWVVLPP51 pKa = 4.15

Molecular weight: 5.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

268

0

268

64229

40

4024

239.7

26.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.958 ± 0.194

0.895 ± 0.082

6.436 ± 0.114

6.128 ± 0.26

4.378 ± 0.126

8.031 ± 0.256

1.543 ± 0.105

6.411 ± 0.128

5.98 ± 0.293

7.072 ± 0.135

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.136 ± 0.129

6.109 ± 0.162

3.76 ± 0.102

3.66 ± 0.083

4.095 ± 0.129

7.451 ± 0.187

7.59 ± 0.338

6.956 ± 0.208

1.081 ± 0.082

4.328 ± 0.125
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski