Ralstonia phage Anchaing

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Anchaingvirus; Ralstonia virus Anchaing

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G5B8D8|A0A7G5B8D8_9CAUD Uncharacterized protein OS=Ralstonia phage Anchaing OX=2759719 GN=A1_00041 PE=4 SV=1
MM1 pKa = 7.34RR2 pKa = 11.84RR3 pKa = 11.84GYY5 pKa = 10.82EE6 pKa = 3.9VMPSEE11 pKa = 4.52LVGMMIRR18 pKa = 11.84EE19 pKa = 3.96ADD21 pKa = 3.81FNDD24 pKa = 4.0DD25 pKa = 3.54GEE27 pKa = 4.51LVLRR31 pKa = 11.84CQDD34 pKa = 3.18QFGVHH39 pKa = 5.96HH40 pKa = 6.94SYY42 pKa = 11.17VEE44 pKa = 4.01EE45 pKa = 4.86GGLLYY50 pKa = 10.53HH51 pKa = 6.65VEE53 pKa = 4.09EE54 pKa = 4.57RR55 pKa = 11.84RR56 pKa = 3.52

Molecular weight: 6.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G5B8F0|A0A7G5B8F0_9CAUD Uncharacterized protein OS=Ralstonia phage Anchaing OX=2759719 GN=A1_00053 PE=4 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84KK3 pKa = 9.49HH4 pKa = 6.4SAQQPTTRR12 pKa = 11.84PTRR15 pKa = 11.84NVGAAILTAVTRR27 pKa = 11.84HH28 pKa = 5.52SPAFVANRR36 pKa = 11.84ALASALARR44 pKa = 11.84AVGLTKK50 pKa = 10.74

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

13484

46

1588

254.4

27.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.718 ± 0.592

0.86 ± 0.183

5.77 ± 0.186

5.607 ± 0.299

3.337 ± 0.19

8.069 ± 0.338

2.091 ± 0.156

4.309 ± 0.215

4.821 ± 0.277

8.447 ± 0.325

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.536 ± 0.151

4.042 ± 0.27

4.316 ± 0.233

4.324 ± 0.302

6.541 ± 0.397

5.725 ± 0.285

6.118 ± 0.302

6.689 ± 0.371

1.632 ± 0.112

3.048 ± 0.244
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski