Yersinia phage PY54
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q7Y3W7|Q7Y3W7_9CAUD Uncharacterized protein OS=Yersinia phage PY54 OX=172667 PE=4 SV=1
MM1 pKa = 7.06 SCGYY5 pKa = 9.85 QGYY8 pKa = 8.9 EE9 pKa = 3.83 FGGGYY14 pKa = 10.14 LDD16 pKa = 3.88 SQCIDD21 pKa = 3.21 GFLWDD26 pKa = 4.3 MDD28 pKa = 4.11 SGGVDD33 pKa = 3.38 SKK35 pKa = 11.86 GDD37 pKa = 3.36 TYY39 pKa = 11.67 LDD41 pKa = 3.6 FGGEE45 pKa = 4.28 IPCPDD50 pKa = 3.93 CNKK53 pKa = 9.32 NAWMAHH59 pKa = 3.99 YY60 pKa = 9.68 RR61 pKa = 11.84 ADD63 pKa = 3.69 IIEE66 pKa = 4.44 IGQEE70 pKa = 3.51 QGFCGKK76 pKa = 9.3 PPKK79 pKa = 10.45 VLMYY83 pKa = 10.3 GGYY86 pKa = 9.42 PEE88 pKa = 4.27 VVRR91 pKa = 11.84 IDD93 pKa = 4.34 LEE95 pKa = 4.46 LEE97 pKa = 4.02 LAA99 pKa = 4.15
Molecular weight: 10.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.917
IPC2_protein 3.999
IPC_protein 3.935
Toseland 3.732
ProMoST 4.088
Dawson 3.923
Bjellqvist 4.075
Wikipedia 3.859
Rodwell 3.757
Grimsley 3.643
Solomon 3.897
Lehninger 3.859
Nozaki 4.037
DTASelect 4.253
Thurlkill 3.783
EMBOSS 3.859
Sillero 4.05
Patrickios 0.21
IPC_peptide 3.897
IPC2_peptide 4.024
IPC2.peptide.svr19 3.945
Protein with the highest isoelectric point:
>tr|Q7Y3X6|Q7Y3X6_9CAUD Prophage repressor OS=Yersinia phage PY54 OX=172667 GN=cI PE=4 SV=1
MM1 pKa = 7.25 GRR3 pKa = 11.84 SNSLANLKK11 pKa = 10.18 ARR13 pKa = 11.84 SKK15 pKa = 11.2 AGIALEE21 pKa = 4.26 FSRR24 pKa = 11.84 CEE26 pKa = 3.94 TPALVIPTRR35 pKa = 11.84 SANALTEE42 pKa = 3.93 NLFLSRR48 pKa = 11.84 ALSSSRR54 pKa = 11.84 RR55 pKa = 11.84 SFSQSPRR62 pKa = 11.84 FFIVEE67 pKa = 4.49 IIDD70 pKa = 3.68 HH71 pKa = 6.03 FCIAGYY77 pKa = 8.36 TPQKK81 pKa = 10.84
Molecular weight: 8.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.35
IPC2_protein 9.458
IPC_protein 10.131
Toseland 10.613
ProMoST 10.423
Dawson 10.701
Bjellqvist 10.409
Wikipedia 10.891
Rodwell 10.877
Grimsley 10.73
Solomon 10.833
Lehninger 10.804
Nozaki 10.628
DTASelect 10.394
Thurlkill 10.613
EMBOSS 11.008
Sillero 10.643
Patrickios 10.716
IPC_peptide 10.833
IPC2_peptide 9.663
IPC2.peptide.svr19 8.578
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
67
0
67
14821
54
1330
221.2
24.47
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.675 ± 0.536
1.39 ± 0.162
5.715 ± 0.235
6.086 ± 0.259
3.563 ± 0.237
6.848 ± 0.308
1.808 ± 0.159
6.538 ± 0.282
5.378 ± 0.282
8.596 ± 0.234
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.672 ± 0.16
4.453 ± 0.204
4.284 ± 0.224
4.17 ± 0.374
5.499 ± 0.264
7.118 ± 0.334
5.917 ± 0.226
5.958 ± 0.25
1.376 ± 0.127
2.955 ± 0.158
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here