Feline foamy virus (FFV) (Feline syncytial virus)
Average proteome isoelectric point is 6.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|O93209|POL_FFV Pro-Pol polyprotein OS=Feline foamy virus OX=53182 GN=pol PE=3 SV=1
MM1 pKa = 7.35 ASKK4 pKa = 10.68 YY5 pKa = 9.96 PEE7 pKa = 4.47 EE8 pKa = 4.82 GPITEE13 pKa = 4.32 GVEE16 pKa = 3.9 EE17 pKa = 4.87 DD18 pKa = 4.31 FNSHH22 pKa = 5.06 STSGLDD28 pKa = 3.29 LTSVGKK34 pKa = 10.26 NPEE37 pKa = 3.76 HH38 pKa = 7.09 PRR40 pKa = 11.84 RR41 pKa = 11.84 ILLVLQHH48 pKa = 6.71 LIAYY52 pKa = 9.96 AEE54 pKa = 4.07 ATIKK58 pKa = 10.48 KK59 pKa = 9.43 QDD61 pKa = 3.46 VPGPLLPILSPYY73 pKa = 8.82 VMAWDD78 pKa = 3.9 NPQNVVTRR86 pKa = 11.84 LVNLGEE92 pKa = 4.14 SWKK95 pKa = 10.62 KK96 pKa = 10.65 YY97 pKa = 10.12 LLSPGWKK104 pKa = 9.86 DD105 pKa = 3.19 CGEE108 pKa = 4.43 RR109 pKa = 11.84 DD110 pKa = 3.29 LTMLTRR116 pKa = 11.84 EE117 pKa = 4.4 LLVPGIGLVQIAATLTKK134 pKa = 10.08 TYY136 pKa = 11.53 VLMCNGRR143 pKa = 11.84 CITGSRR149 pKa = 11.84 TDD151 pKa = 4.09 PDD153 pKa = 4.59 CDD155 pKa = 3.64 PLFCKK160 pKa = 10.36 LLCWKK165 pKa = 10.5 QNIQDD170 pKa = 3.66 PRR172 pKa = 11.84 EE173 pKa = 4.22 CNLEE177 pKa = 3.96 EE178 pKa = 3.82 WCLYY182 pKa = 10.97 SLDD185 pKa = 4.64 PEE187 pKa = 5.24 HH188 pKa = 7.78 DD189 pKa = 4.22 PLWDD193 pKa = 3.18 PKK195 pKa = 10.5 MIVRR199 pKa = 11.84 RR200 pKa = 11.84 HH201 pKa = 5.87 RR202 pKa = 11.84 NLLPYY207 pKa = 10.41 CMRR210 pKa = 11.84 PFLIWMNYY218 pKa = 8.54 ISHH221 pKa = 6.89 NPLTQQCIMMKK232 pKa = 8.51 TLNMLWRR239 pKa = 11.84 AQADD243 pKa = 3.93 DD244 pKa = 4.37 PSDD247 pKa = 3.67 VASLYY252 pKa = 10.33 PRR254 pKa = 11.84 VKK256 pKa = 10.34 VFKK259 pKa = 10.34 ASHH262 pKa = 6.08 FDD264 pKa = 3.25 IFGSASGNSEE274 pKa = 4.33 EE275 pKa = 4.11 RR276 pKa = 11.84 VSWAKK281 pKa = 10.44 EE282 pKa = 3.45 NSHH285 pKa = 6.72 RR286 pKa = 11.84 GEE288 pKa = 4.06 YY289 pKa = 10.47 SLLPSSDD296 pKa = 3.93 DD297 pKa = 3.62 EE298 pKa = 4.32 EE299 pKa = 5.66 EE300 pKa = 4.2 EE301 pKa = 3.88 MSEE304 pKa = 4.9 RR305 pKa = 11.84 EE306 pKa = 3.99 EE307 pKa = 4.05 LLCHH311 pKa = 7.32 INQCQQKK318 pKa = 9.64 LFYY321 pKa = 10.34 PGGTTDD327 pKa = 3.53 VLGMEE332 pKa = 4.57 SNVWLTKK339 pKa = 9.95 FVNIKK344 pKa = 9.77 FPKK347 pKa = 7.17 GTKK350 pKa = 9.96 VILPDD355 pKa = 3.17 GRR357 pKa = 11.84 KK358 pKa = 9.46 FIACDD363 pKa = 3.68 PEE365 pKa = 4.86 LKK367 pKa = 10.38 PLLQEE372 pKa = 4.94 LKK374 pKa = 10.49 FLDD377 pKa = 3.98 RR378 pKa = 11.84 ATSEE382 pKa = 4.33 SSDD385 pKa = 3.71 SEE387 pKa = 4.27
Molecular weight: 44.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.148
IPC2_protein 5.219
IPC_protein 5.181
Toseland 5.194
ProMoST 5.283
Dawson 5.219
Bjellqvist 5.347
Wikipedia 5.105
Rodwell 5.156
Grimsley 5.143
Solomon 5.219
Lehninger 5.181
Nozaki 5.372
DTASelect 5.512
Thurlkill 5.245
EMBOSS 5.194
Sillero 5.448
Patrickios 3.923
IPC_peptide 5.232
IPC2_peptide 5.448
IPC2.peptide.svr19 5.389
Protein with the highest isoelectric point:
>sp|O56861|ENV_FFV Envelope glycoprotein gp130 OS=Feline foamy virus OX=53182 GN=env PE=1 SV=1
MM1 pKa = 7.42 ARR3 pKa = 11.84 EE4 pKa = 4.23 LNPLQLQQLYY14 pKa = 10.05 INNGLQPNPGHH25 pKa = 6.78 GDD27 pKa = 3.61 IIAVRR32 pKa = 11.84 FTGGPWGPGDD42 pKa = 2.77 RR43 pKa = 11.84 WARR46 pKa = 11.84 VTIRR50 pKa = 11.84 LQDD53 pKa = 3.4 NTGQPLQVPGYY64 pKa = 9.02 DD65 pKa = 4.03 LEE67 pKa = 5.24 PGIINLRR74 pKa = 11.84 EE75 pKa = 4.75 DD76 pKa = 3.22 ILIAGPYY83 pKa = 9.54 NLIRR87 pKa = 11.84 TAFLDD92 pKa = 3.77 LEE94 pKa = 4.41 PARR97 pKa = 11.84 GPEE100 pKa = 3.59 RR101 pKa = 11.84 HH102 pKa = 6.28 GPFGDD107 pKa = 3.98 GRR109 pKa = 11.84 LQPGDD114 pKa = 3.59 GLSEE118 pKa = 4.43 GFQPITDD125 pKa = 3.89 EE126 pKa = 4.63 EE127 pKa = 4.24 IQAEE131 pKa = 4.39 VGTIGAARR139 pKa = 11.84 NEE141 pKa = 3.61 IRR143 pKa = 11.84 LLRR146 pKa = 11.84 EE147 pKa = 3.58 ALQRR151 pKa = 11.84 LQAGGVGRR159 pKa = 11.84 PIPGAVLQPQPVIGPVIPINHH180 pKa = 6.27 LRR182 pKa = 11.84 SVIGNTPPNPRR193 pKa = 11.84 DD194 pKa = 3.24 VALWLGRR201 pKa = 11.84 STAAIEE207 pKa = 4.46 GVFPIVDD214 pKa = 3.25 QVTRR218 pKa = 11.84 MRR220 pKa = 11.84 VVNALVASHH229 pKa = 7.41 PGLTLTEE236 pKa = 4.26 NEE238 pKa = 4.09 AGSWNAAISALWRR251 pKa = 11.84 KK252 pKa = 8.92 AHH254 pKa = 6.16 GAAAQHH260 pKa = 5.97 EE261 pKa = 4.53 LAGVLSDD268 pKa = 3.44 INKK271 pKa = 9.9 KK272 pKa = 10.51 EE273 pKa = 4.34 GIQTAFNLGMQFTDD287 pKa = 4.5 GNWSLVWGIIRR298 pKa = 11.84 TLLPGQALVTNAQSQFDD315 pKa = 4.18 LMGDD319 pKa = 4.13 DD320 pKa = 3.42 IQRR323 pKa = 11.84 AEE325 pKa = 4.01 NFPRR329 pKa = 11.84 VINNLYY335 pKa = 9.96 TMLGLNIHH343 pKa = 5.95 GQSIRR348 pKa = 11.84 PRR350 pKa = 11.84 VQTQPLQTRR359 pKa = 11.84 PRR361 pKa = 11.84 NPGRR365 pKa = 11.84 SQQGQLNQPRR375 pKa = 11.84 PQNRR379 pKa = 11.84 ANQSYY384 pKa = 9.76 RR385 pKa = 11.84 PPRR388 pKa = 11.84 QQQQHH393 pKa = 5.87 SDD395 pKa = 3.42 VPEE398 pKa = 3.82 QRR400 pKa = 11.84 DD401 pKa = 3.4 QRR403 pKa = 11.84 GPSQPPRR410 pKa = 11.84 GSGGGYY416 pKa = 8.6 NFRR419 pKa = 11.84 RR420 pKa = 11.84 NPQQPQRR427 pKa = 11.84 YY428 pKa = 7.97 GQGPPGPNPYY438 pKa = 10.03 RR439 pKa = 11.84 RR440 pKa = 11.84 FGDD443 pKa = 3.53 GGNPQQQGPPPNRR456 pKa = 11.84 GPDD459 pKa = 3.16 QGPRR463 pKa = 11.84 PGGNPRR469 pKa = 11.84 GGGRR473 pKa = 11.84 GQGPRR478 pKa = 11.84 NGGGSAAAVHH488 pKa = 5.29 TVKK491 pKa = 10.86 ASEE494 pKa = 4.19 NEE496 pKa = 4.34 TKK498 pKa = 10.65 NGSAEE503 pKa = 4.0 AVDD506 pKa = 3.95 GGKK509 pKa = 10.3 KK510 pKa = 9.93 GGKK513 pKa = 9.22 DD514 pKa = 2.99
Molecular weight: 55.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.289
IPC2_protein 9.355
IPC_protein 10.218
Toseland 10.204
ProMoST 10.028
Dawson 10.394
Bjellqvist 10.145
Wikipedia 10.643
Rodwell 10.452
Grimsley 10.482
Solomon 10.496
Lehninger 10.452
Nozaki 10.175
DTASelect 10.145
Thurlkill 10.262
EMBOSS 10.628
Sillero 10.321
Patrickios 9.955
IPC_peptide 10.496
IPC2_peptide 8.96
IPC2.peptide.svr19 8.715
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
1
6
3561
209
1156
593.5
67.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.521 ± 0.578
1.91 ± 0.613
4.802 ± 0.357
6.094 ± 0.541
3.061 ± 0.249
6.712 ± 1.555
2.443 ± 0.19
6.403 ± 0.718
6.375 ± 1.129
9.997 ± 0.595
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.825 ± 0.357
5.392 ± 0.312
6.712 ± 0.807
5.336 ± 0.826
4.999 ± 0.846
6.15 ± 0.634
5.981 ± 0.544
5.532 ± 0.378
2.19 ± 0.224
3.566 ± 0.511
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here