Gregarina niphandrodes (Septate eugregarine)

Taxonomy: cellular organisms; Eukaryota; Sar; Alveolata; Apicomplexa; Conoidasida; Gregarinasina; Eugregarinorida; Gregarinidae; Gregarina

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6341 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A023B553|A0A023B553_GRENI PLD-like domain protein OS=Gregarina niphandrodes OX=110365 GN=GNI_095850 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 10.5CLGAFFASVVLVSGGLSTYY21 pKa = 11.01SDD23 pKa = 3.69TCFLPIDD30 pKa = 4.16LLLVQDD36 pKa = 3.99TTGSFMDD43 pKa = 4.84DD44 pKa = 3.5LPNVSKK50 pKa = 10.99AIPTLVSSVLEE61 pKa = 4.21NNPGSHH67 pKa = 6.84FGAVEE72 pKa = 4.43FKK74 pKa = 10.71DD75 pKa = 3.51KK76 pKa = 10.56PYY78 pKa = 10.75RR79 pKa = 11.84DD80 pKa = 3.79LGEE83 pKa = 5.03PDD85 pKa = 3.59DD86 pKa = 3.89FCYY89 pKa = 10.4RR90 pKa = 11.84MASALTGDD98 pKa = 3.3VATFQKK104 pKa = 10.83AWDD107 pKa = 3.72TLYY110 pKa = 11.32ASGGGDD116 pKa = 3.36LPEE119 pKa = 4.43VQLQALIDD127 pKa = 3.98AAQDD131 pKa = 3.43PTVGWRR137 pKa = 11.84VIDD140 pKa = 4.08NSIQSGDD147 pKa = 3.78DD148 pKa = 3.32VSSVVGARR156 pKa = 11.84LIVLSTDD163 pKa = 3.72AVPHH167 pKa = 7.06DD168 pKa = 4.25PLDD171 pKa = 3.75YY172 pKa = 10.98DD173 pKa = 4.75LYY175 pKa = 11.25LEE177 pKa = 4.84EE178 pKa = 5.33PLPEE182 pKa = 4.81PPLPPSFMPPFPDD195 pKa = 2.9NSVGVTPDD203 pKa = 3.76FQCRR207 pKa = 11.84IYY209 pKa = 10.38DD210 pKa = 4.0YY211 pKa = 11.23PSMAQAANVLKK222 pKa = 9.32TNNVMVVFLVPEE234 pKa = 4.22IEE236 pKa = 4.12TDD238 pKa = 4.61AVAKK242 pKa = 9.69WSEE245 pKa = 4.08FNTEE249 pKa = 4.08YY250 pKa = 10.85LGQPANFLQYY260 pKa = 10.3ISSDD264 pKa = 3.11SSDD267 pKa = 2.71IVQVVLQAVSAVSQYY282 pKa = 11.22VCFF285 pKa = 4.38

Molecular weight: 30.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A023BA32|A0A023BA32_GRENI C2 domain-containing protein OS=Gregarina niphandrodes OX=110365 GN=GNI_042550 PE=4 SV=1
KKK2 pKa = 10.1ATLQGLAHHH11 pKa = 6.63ALCKKK16 pKa = 6.11HHH18 pKa = 7.17KK19 pKa = 10.43LRR21 pKa = 11.84QNAAHHH27 pKa = 6.87PVVPIPHHH35 pKa = 7.38SRR37 pKa = 11.84RR38 pKa = 11.84PPTLPGPRR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84ALLHHH53 pKa = 6.22PQMPIHHH60 pKa = 6.15HH61 pKa = 6.54VFPSPHHH68 pKa = 6.44LRR70 pKa = 11.84FRR72 pKa = 11.84LRR74 pKa = 11.84FASHHH79 pKa = 6.99LRR81 pKa = 11.84FRR83 pKa = 11.84LRR85 pKa = 11.84FASHHH90 pKa = 6.87FRR92 pKa = 11.84FRR94 pKa = 11.84LQFASRR100 pKa = 11.84FGHHH104 pKa = 5.19RR105 pKa = 11.84

Molecular weight: 12.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6341

0

6341

2913775

23

5952

459.5

50.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.541 ± 0.035

1.927 ± 0.015

5.773 ± 0.022

6.571 ± 0.03

3.196 ± 0.016

7.034 ± 0.033

2.319 ± 0.012

4.067 ± 0.026

4.657 ± 0.029

9.476 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.071 ± 0.012

3.416 ± 0.02

5.748 ± 0.032

4.017 ± 0.02

6.567 ± 0.026

7.697 ± 0.031

5.978 ± 0.025

6.966 ± 0.022

1.382 ± 0.011

2.595 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski